Questions tagged [antibody]
A protein produced by the B-cells of the immune system which binds to a particular antigen, a foreign substance to the body.
142
questions
5
votes
0answers
57 views
Are autoantibodies against intracellular proteins “functional”?
Autoantibodies against intracellular proteins have been detected in some autoimmune diseases (For example, TRIM21 in Sjögren's syndrome and NALP5 in Autoimmune Polyendocrine Syndrome Type 1).
My ...
-1
votes
2answers
28 views
Detecting multiple compounds on a lateral flow immunoassay – is this a case of monoclonal vs polyclonal antibodies?
I am working on developing lateral flow immunoassays for drugs of abuse and needed some advice on detecting multiple drug compounds in one immunoassay.
As an example, an existing LFIA on the market ...
2
votes
1answer
110 views
How long after taking a vaccine shot (Oxford–AstraZeneca) could an individual test negative for antibody (IgM)?
What I know so far about the way this vaccine works is that by encoding virus' biological signature into the vaccine and injecting it into a subject, the subject's immune system would learn to ...
1
vote
1answer
30 views
How to identify plasma cells that only produce monoclonal antibodies?
I am studying the procedures of forming hybridoma cells for generating a large number of monoclonal antibodies.
Before the procedure of fusion (with multiple myeloma cells) happens, I would like to ...
-2
votes
1answer
52 views
Why is the antibody light chain essential for function?
Humans and most other mammals produce antibodies consisting of two heavy chains, each linked to a light chain. Both heavy and light chain contribute to the variable region, and thus the antigen ...
3
votes
0answers
98 views
Do COVID-19 vaccines produce more spike protein than natural infection?
The SARS-CoV-2 spike protein has been shown to be harmful on its own.
However, a news article quoted an "expert" as saying
The spike protein components of the vaccine are not produced in ...
5
votes
2answers
70 views
Why do T cells have MHC II receptors?
I have seen the answer to this question which says that T cells do not express MHC II proteins which would make sense.
However, my textbook "The immune system" by Peter Parham disagrees. It ...
3
votes
2answers
68 views
Why is there not an immune response to injected immunoglobulins?
When you inject immunoglobulins as a treatment for certain diseases, the immunoglobulins are a foreign substance.
I can appreciate that maybe the constant region would be similar to the hosts as it is ...
37
votes
1answer
4k views
Are the antibodies developed by differing vaccines still the same?
If one person gets an mRNA vaccine that teaches their body to deal with a specific virus, and another person gets a similar but different mRNA vaccine, and another person gets a more "classic&...
1
vote
1answer
39 views
Do phagocytes need antibodies to be able to engulf pathogens (to function)?
I recently saw a question about monoclonal antibodies, that are specific to a certain virus, being split (into their constant and variable regions via an enzyme), and the question asked whether some ...
1
vote
0answers
41 views
What differentiates bound antibodies from unbound ones?
When an antibody is bound to an antigen, it can then stimulate a FcR receptor on a phagocyte etc. to respond to the threat. What stops 'free' antibodies from spuriously activating an immune response?
...
1
vote
1answer
42 views
Are antibodies removed before blood transfusion
I am an O blood group person meaning, I can donate my blood to all as I don't have any Antigen A and B. But my body does contain antibodies A and B right?
If they were along with the donor blood, ...
7
votes
2answers
43 views
Meaning of “stripped” in “stripped antibody-depleted sera”
From a research paper:
FOLR1 autoantibody detection
The assay for identification of the presence and relative quantification of FOLR1 autoantibodies in serum samples was performed as previously ...
-1
votes
2answers
147 views
How can mutation of viruses lead to loss of fit to antibodies without loss of fit to antigen of cells they infect?
Viruses are known to mutate, thereby escaping immune cells and evading vaccination. Given that there is one and the same specificity of the key to both the receptor on the infected cell causing the ...
0
votes
2answers
84 views
Sequence structure of antibodies
It is said that
"When a virus or a pathogen enters the body, the immune-response produces Y-shaped proteins called antibodies to bind to the pathogen or virus".
"Also it is possible to ...
1
vote
0answers
19 views
Blood serum transfusions - could vaccines against the anti-A/anti-B etc. antibodies be derived, at least in theory?
People with the AB-positive blood type have all three of the A, B and RhD antigens present on their red blood cells, and no antibodies to these in their blood serum (aka blood plasma.)
This means that ...
0
votes
0answers
8 views
Does germinal centre interaction with environment contributes by any means to the heterogeneity of the SHM-resulted antibody repertoire?
B-cells undergo a process of rapid mutagenesis and division which is followed by selection. Somatic hypermutation (SHM) produces many different antibody (Ab) genes that can potentially be useful ...
3
votes
2answers
48 views
What is a function-triggering antibody?
I am reading a journal paper on the effect of a neural cell adhesion molecule on neuronal development and in the abstract of this paper I have come across the following:
The autophosphorylation and ...
5
votes
0answers
65 views
Can any molecule become a hapten?
Hapten are small-molecules, that can only become immunogenic when conjugated with a carrier protein. I was wondering if all small-molecules can become haptens (eg. by synthetic conjugation).
Given ...
0
votes
0answers
24 views
Problem with epoxy silane (Random results in glass silanization). What are crucial points to look at?
I`m trying to immobilize an antibody onto the glass surface by means of GPTOMS (glycidoxypropyl trimetoxy silane). Using the same protocol (silanization in toluene or acidified ethanol (see below)) I ...
1
vote
1answer
27 views
What does the decline of serum antibodies after infection mean for B cell immunity, exactly?
About a month ago there was a small media blip about a report in the New England Journal of Medicine that neutralizing antibodies to SARS-CoV-2 decline significantly within a matter of months. ...
2
votes
0answers
11 views
What is the mechanism responsible for long-lasting detectable antibody titers?
As I understand, when naive B cell encounters antigen matching its receptors and is activated by a T helper cell, it can either differentiate into 4 plasma cells, produce a lot of antibodies and ...
1
vote
1answer
26 views
TMB vs ECL for ELISA: which detection method is more sensitive?
I failed to find a comparison, does anyone know which ELISA detection method is more sensitive of these two: ECL, TMB?
Many thanks!
-3
votes
2answers
100 views
Can we develop a virus which is amicable for us but deadly for SARS-CoV-2 and HIV?
I am not biologist and do not have special education in it etc.
It is know that in the wild, some species have their deadly enemies.
What I am suggesting is can we humans find/create virus that is ...
0
votes
1answer
49 views
Easy and cheap antigen/antibody couple
For an application I need to find a cheap antigen and cheap correspondent antibody. The antigen can be literally any molecule that is cheap and potentially easy to produce and with a correspondent ...
11
votes
1answer
633 views
Why aren't the antibodies in the mother's milk digested by the infant's digestive system?
Why aren't the antibodies in the mother's milk digested by the infant's digestive system?
Enzymes like pepsin have been found to be present in infant's digestive system(1). Though the pH in the ...
3
votes
1answer
47 views
Can I use fluorophore conjugated antibody as neutralizing antibody?
I need to order a neutralizing antibody to block a protein of interest.
The pure grade functioning antibody (no conjugates) is substantially more expensive than the fluorophore conjugatated ones.
Can ...
1
vote
1answer
54 views
Are all antibodies against a common antigen identical? [duplicate]
I understand that when some antigen (e.g., virus, bacteria, etc.) is recognized in the body, antibodies specific to this antigen are produced that, in turn, bind to the antigen and effectively ...
3
votes
1answer
190 views
Why are antibodies Y-shaped?
I generally know how antibodies work by binding to antigens, but what is the specific purpose to being Y-shaped, as opposed to any other shape? Does this aid their function? Thank you.
1
vote
1answer
36 views
How does the choice of blood draw site influence the possible specificity of a serological test?
The news has reported that a new serological test for the presence of anti-SARS-CoV-2 antibodies has received an emergency use authorization from the FDA, and notably has a higher specificity than ...
0
votes
0answers
18 views
How antibodies are produced in our body against intracellular proteins of infectious bacteria?
When an infectious agent invades our body, then surface antigens of the infectious agent are detected by our immune system and B-cells get activated. However, we do have antibodies in our blood ...
0
votes
0answers
29 views
Why do antibody tests target different antibodies?
I've noticed in various COVID-19 lateral flow assays, they seem to target different antibodies (S1, S2, NP). What are the benefits or downsides of choosing different antibodies? I know that some have ...
1
vote
0answers
43 views
To what exten do children produce antibodies against covid-19?
Here in Sweden all kindergartens and schools up to the month of June the year people turn 16 remain open. In almost all other European country schools are closed.
I do not really know why our schools ...
3
votes
1answer
109 views
What determines if a small protein / large peptide is immunogenic?
I'm wondering if there is some threshold in size or a specific structural property that determines if a small protein or large peptide would cause an immune reaction.
Context: there are a number of ...
-2
votes
1answer
38 views
Can someone donate antibodies? [closed]
Consider the following scenario:
Person A has been infected with some virus (for example, SARS-CoV-2)
Person A has recovered
From this scenario, I assume Person A has managed to develop antibodies ...
1
vote
1answer
34 views
How to filter out the antibody light chains into kappa and lambda types on the PDB website?
The antibody light chains can either be kappa or lambda types. I am searching homologous sequences on the RCSB PDB website. Is there a way to filter out these two types?
1
vote
1answer
54 views
How are antibodies specific for a disease detected in the blood if everybody produces a different antibody for the same antigen?
To break the title down into parts:
There exist serology tests that detect the amount of an antibody (Ab)
against a specific pathogen/antigen.
Every human produces their own Ab for a specific ...
0
votes
0answers
60 views
How does the immune system find the DNA code for antibodies
There are millions of possible antigens. To respond to each antigen, the immune system must quickly produce an antibody by modifying the DNA of a B cell.
I have no idea how this process works, but ...
2
votes
0answers
42 views
Does antibodies get produced in female body against sperms?
Our immune system produces antibodies against any foreign particles entering our body.In female body sperms are a foreign particle .Does females produce antibodies or some sort of chemical resistance ...
3
votes
2answers
281 views
What does it mean to be a fully human monoclonal antibody?
I somewhat understand that some monoclonal antibodies are developed from the cells of mice, or a fusion of human and mice genes.
When something is a fully human monoclonal antibody does that mean it ...
2
votes
1answer
61 views
Where does the number 67 in the nuclear protein/antigen Ki-67 come from? Why not 66 or 68?
I have read in in the original paper that in the year 1983 a research group in Kiel, Germany (that's where the Ki- in the name comes from) developed monoclonal mouse antibodies against Hodgkin ...
1
vote
1answer
28 views
Protein A-antibody SDS-PAGE
I wanted to know whether the boiling of Protein A- antibody complex for SDS-PAGE analysis will disrupt the interaction? and result in dissociation of protein A and antibody?
Thank you
-5
votes
1answer
159 views
Chirality of anti-bodies
Do anti-bodies in the blood stream present chirality?
I am interested in this question from a mathematical and physical point of view. A person allowed to travel through a fourth spatial dimension is ...
2
votes
1answer
3k views
Meaning of units in ELISA based tests?
For some ELISA based antibody tests (e.g. h-tTg antibody test), labs report units as RU/mL or U/mL. Also different labs have different cut off (normal range) values.
I understand that different kit ...
2
votes
1answer
44 views
Can the body always find the right antibodies?
If the body produces random antibodies in order to fight a virus, is there a small chance that the specific antibody needed to fight the virus just won't be randomly generated? Even though it is a ...
-2
votes
1answer
37 views
Can monoclonal antibodies work only if the target of it is located in the cell's surface?
Can monoclonal antibodies work only if the target of it is located in the cell's surface?
or it can be used to targeting a protein located inside the cell?
0
votes
0answers
19 views
Re-stain slice using a different secondary antibody?
I stained a free-floating ~300uM brian slice using 4 different 1º antibodies (rabbit, chick, mouse, rat).
For the mouse primary, I mistakenly used an Alexa-350 secondary, when I should have used 594.
...
1
vote
2answers
3k views
What is a “pan-specific” antibody?
I am new to biology. I searched a lot to find an article that explains what "pan-specific" antibody is but I could not find anything substantial that would help me understand what it is.
An example ...
2
votes
1answer
68 views
How are primary monoclonal antibodies for screening mutant cells made, physically?
I'm working with a fairly common protein expressed in a large numbers of organisms, let's say a keratin-associated beta-protein. I'm trying to develop a process which requires primary-secondary ...
1
vote
1answer
700 views
Can multiple antibodies bind the same antigen?
Given the size difference between small molecule antigens and antibodies is it ever possible for multiple antibodies to bind to the same antigen if they recognize different domains on that antigen? ...
