Questions tagged [antibody]
A protein produced by the B-cells of the immune system which binds to a particular antigen, a foreign substance to the body.
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Recommendation for ChIP-seq antibody (CEBPa) in mouse
I am looking for a recommendation for a ChIP-seq antibody towards CEBPa in mice. Currently using the D-5 from Santa Cruz (sc-365318) but yields after pulldown are more than modest. Published data were ...
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What does it mean to be a fully human monoclonal antibody?
I somewhat understand that some monoclonal antibodies are developed from the cells of mice, or a fusion of human and mice genes.
When something is a fully human monoclonal antibody does that mean it ...
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1answer
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Where does the number 67 in the nuclear protein/antigen Ki-67 come from? Why not 66 or 68?
I have read in in the original paper that in the year 1983 a research group in Kiel, Germany (that's where the Ki- in the name comes from) developed monoclonal mouse antibodies against Hodgkin ...
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Protein A-antibody SDS-PAGE
I wanted to know whether the boiling of Protein A- antibody complex for SDS-PAGE analysis will disrupt the interaction? and result in dissociation of protein A and antibody?
Thank you
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Difference between scFV vs scTCR
Both scFv and scTCR consist of 2 variable regions joined by a linker loop. The 2 variable regions both consist of 3 CDR regions encompassed by 4 framework regions. So what's the difference ...
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1answer
62 views
Chirality of anti-bodies
Do anti-bodies in the blood stream present chirality?
I am interested in this question from a mathematical and physical point of view. A person allowed to travel through a fourth spatial dimension is ...
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Presence of anti-drug-antibodies in patients prior to drug exposure (Mabs)?
How do we explain the presence of anti-drug-antibodies in patients prior to exposure to a drug - specifically for monoclonal antibodies (Mabs)?
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Meaning of units in ELISA based tests?
For some ELISA based antibody tests (e.g. h-tTg antibody test), labs report units as RU/mL or U/mL. Also different labs have different cut off (normal range) values.
I understand that different kit ...
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1answer
38 views
Can the body always find the right antibodies?
If the body produces random antibodies in order to fight a virus, is there a small chance that the specific antibody needed to fight the virus just won't be randomly generated? Even though it is a ...
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Can monoclonal antibodies work only if the target of it is located in the cell's surface?
Can monoclonal antibodies work only if the target of it is located in the cell's surface?
or it can be used to targeting a protein located inside the cell?
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What is the advantage of producing IgY bird's antibody instead of IgG? [closed]
Usually IgG antibodies are produced in mice. What is the advantage of producing IgY bird's antibody instead of IgG?
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Re-stain slice using a different secondary antibody?
I stained a free-floating ~300uM brian slice using 4 different 1º antibodies (rabbit, chick, mouse, rat).
For the mouse primary, I mistakenly used an Alexa-350 secondary, when I should have used 594.
...
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2answers
722 views
What is a “pan-specific” antibody?
I am new to biology. I searched a lot to find an article that explains what "pan-specific" antibody is but I could not find anything substantial that would help me understand what it is.
An example ...
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PU.1 ChIP-grade antibody
We have been using a ChIP-grade antibody for PU.1 ChIP-seq (human/mouse) from Santa Cruz that has now been discontinued. Has anyone a current and hands-on-based (...
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1answer
59 views
How are primary monoclonal antibodies for screening mutant cells made, physically?
I'm working with a fairly common protein expressed in a large numbers of organisms, let's say a keratin-associated beta-protein. I'm trying to develop a process which requires primary-secondary ...
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Are there multiple IgY subclasses?
I know that there are several subclasses of IgG, but can't track down anything on whether or not IgY similarly has subclasses.
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1answer
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Can multiple antibodies bind the same antigen?
Given the size difference between small molecule antigens and antibodies is it ever possible for multiple antibodies to bind to the same antigen if they recognize different domains on that antigen? ...
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Antibody staining
I am staining tissue sections and I did a mistake, I was supposed to have mixed 3 primary antibodies but I stained only with one of them. After the 1h incubation I washed 10 min with PBS and then I ...
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How to know which residues belong to the variable or constant regions of a Fab (fragment antibody)?
I have a fragment of antibody (Fab) structure, and I know its amino acid sequences of its heavy chain and light chain. As each chain of Fab can be divided into variable and constant regions, how can I ...
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Is antivenom fatal?
Antivenom is used for curing snake bites. Is it fatal when used without being bitten by a snake?
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Why does Anti-A antibodies make type-A blood type clump?
A-type blood has B-antibodies; it also clumps anti-A antibodies are inserted. Why is that? The blood has no antibodies against the A-antibodies to make clump in this way.
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Number of different clones of B lymphocytes
My professor told us that there are about a million different B cells based on their surface receptor.
I have read that we have about 30000 genes in all. Since receptors are proteins how do these ...
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Do antibodies to an arbovirus in a bloodmeal reduce vector competence?
When we conduct mosquito infection studies we typically mix blood and virus rather than using viraemic blood from naturally infected hosts. I'm looking for a paper I read some years ago which ...
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81 views
How does antibody structure relate to function? [closed]
I know that antibodies have 2 binding sites (as Y shape) so they can bind to 2 pathogens for agglutination. They have a hinge, heavy and light chains, constant and variable sections on these chains.
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1answer
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IgA-s in an immune system vaccined intramuscularly against Hepatisis A
As IgA are immunoglobulins associated with secretion and mucosis membranes, I am interested whether after intramuscular vaccination with Hepatitis A vaccine IgA antibodies will be produced by the ...
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What do memory cells actually do?
So I know that memory cells "remember" the most efficient way to kill a pathogen should it show up again... but what is the mechanism by which memory cells become activated by the second contact with ...
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How do I name a binding antibody? “Binding antibody to”, “binding antibody against”, or “anti-[antigen] binding antibody”?
I'm translating a text that describes how an immunogenicity of a drug is measured by assaying the levels of binding antibodies to the drug. Or is it "against the drug"? I'm wondering how to name these ...
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Will a sandwich assay work with a GST 26 antibody/protein?
I have a GST 26 kda antibody, and want to use it in a sandwich lateral flow assay. Are there enough epitope regions for both antibodies to bind to the protein?
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Will a polyclonal antibody attach to proteins of different kDA?
If I have a GST 26kDa polyclonal antibody, will it bind to the GST 28kDa protein as well?
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355 views
How do scientists discover a new antigen and its epitope?
I've found some database on the internet that list all discovered antigens and their epitopes.
So how do scientists discover a new antigen? Do they try to inject them into the body to see if it ...
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1answer
77 views
Detect which antigen binds to IgE
Assume that a patient has chronic hives (itchy) on the skin. I understand that he has an antigen, which binds to IgE and eventually triggers histamine release, causing an allergic reaction. So is ...
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1answer
78 views
Rationale behind washing off unbound protein antigen in indirect ELISA
My question is with regards to the usability of the indirect ELISA in measuring protein concentration. Suppose we want to quantify the concentration of a protein. The indirect ELISA method would ...
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1answer
62 views
Difference between ‘tagging’ and ‘conjugating’ a fluorochrome to an antibody?
The Wikipedia entry on fluorescence repeatedly states that “a fluorochrome must be tagged or conjugated to the antibody”.
How is tagged or conjugated different? Is this a mistake or are these indeed ...
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1answer
90 views
How can antibodies in rapid antibody tests be kept at room temperature without denaturing?
The normal temperature for long term antibody storage is around -20 degrees Celsius. However, when developed into rapid antibody tests, they can be kept at room temperature for prolonged periods. How ...
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Why don't phagocytes eliminate mutualistic foregin organisms residing in our body?
Many organisms residing in our body and have a symbiotic mutualistic relationship with our body e.g. organisms in our small intestines. How come our body does not activate an immune response against ...
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Does the saliva of a person just recovered from an infectious disease help to cure another persons having the same disease?
I have always had this 'weird' thought (But could never quite test it...). Would the saliva of a just recovered person contains antibodies, or other immuno-boosting substances that can help fight off ...
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About sandwich ELISA
In sandwich ELISA the Fc region of primary antibody bind to the polystyrene coated well. But what are the specific interactions (like 'hydrophobic interaction' or 'van der waals force') happen between ...
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1answer
841 views
How does our body produce new antibodies?
My professor said that in human body nearly 10$^9$-10$^1$$^2$ antibodies are produced by VDJ recombination and all the antigens that a person encounters in his lifetime are dealt by antibodies ...
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How are antibodies extracted from donor blood?
When blood is donated, the antibodies within it are extracted, but how exactly do they do it? How do they take out the antibodies within the blood, what process do they go through?
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1answer
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After getting fully vaccinated for HBV why does the antiHBs titer last for so long?
When a newborn child is fully vaccinated with HBV and let's assume he's responsive, his antiHBs titers will be high. But since the antigenic stimulus is withdrawn, shouldn't the titre fall to nill in ...
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What is the use of an antibody that is non-neutralizing?
When we generally speak of the immune response to viral infections, we talk of neutralising antibodies. These are antibodies that can neutralise the effect of the virus and reduce its load.
My ...
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1answer
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Meaning of “Antibody is 50% w/w pure of interfering proteins”
From Google Patents, "Preparing antibodies from cho cell cultures for conjugation":
An isolated antibody or ADC is typically at least 50% w/w pure of interfering proteins and other contaminants ...
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Does denaturing proteins lead to loss of epitopes?
I am doing an experiment where I have to do both Immunohistochemistry and SDS-PAGE. I am assuming that the native conformation of the protein is maintained in the IHC. But during the blot, we heat the ...
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1answer
719 views
What is the rationale behind IgM being the default antibody?
I know that the$\ C _\mu $ gene appears first in line for class switching and hence the IgM is the default antibody. But what is the rationale for it being so? There must be some advantage (...
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Using only one antibody to detect COX-2 enzyme
If I wanted to detect COX-2 from a western blot test, would only a primary antibody work? I'm on a budget so does anyone know of relatively cheap COX-2 antibodies?
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B-cell antibody production
I've just learned about B cells in immunology lectures and some things are not clear to me.
Here's what I know:
1) Apparently, each B cell produces a specific antibody, determined randomly at the ...
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1answer
817 views
What is A in IgA? [duplicate]
What is the significance of letter A in immunoglobulinA?
What are the significance of other letters D, M , G, E in diffeternt types of antibodies?
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398 views
Blood antigens and immune response
In my textbook, the definition of an antigen is written as follows:
Antigen: A substance that the body recognises as foreign and that can evoke an immune response
The following image also confused ...
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2answers
103 views
Can ELISA be used to detect a plant enzyme? Creating assay for a new enzyme
If the goal is to generate a rapid assay for an enzyme of plant source what are the typical options?
i.e. Could one do something like: Generate an antibody to the enzyme and then use it to create an ...
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Why does the blood not clump and result in death?
In this question's accepted answer, it is said that the blood type will slowly change to that of the donor's.
When the blood in the person is about 50% his own and 50% that of the donor's.
i.e, 50% A ...
