All Questions
Tagged with antibody biochemistry
15 questions
2
votes
1
answer
64
views
Improving contrast between dot and paper in dot blot
Currently using dot blot to attempt to determine if a serum contains antibodies for some reagents I am testing.
I pipetted samples as 10-5ul dots on whatman paper.
Incubation steps were for 1 hour ...
- 21
0
votes
1
answer
30
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Why do monoclonal and polyclonal antibodies behave differently as labeled secondary antibodies?
According to the MBL guide to generating antibodies, the differences between monoclonal and polyclonal antibody extend beyond the fact that the latter is heterogeneous, binding to various epitopes for ...
- 3
1
vote
0
answers
71
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Antibody structural determinants of epitope size
I am curious whether there are structural correlates in antibodies that relate not to epitope sequence but to epitope size. Specifically, I imagine that the antibody-epitope interface size is ...
- 10.8k
0
votes
0
answers
30
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PEGylation safety and hybridomas
PEGylation is the covalent attachment of PEG (PolyEthylene Glycol) to molecules (e.g. proteins). It states in wikipedia ("PEGylation"):
The covalent attachment of PEG to a drug or ...
- 141
4
votes
1
answer
552
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How does one derive a KD from an equilibrium titration experiment?
If I have an antibody A and a target B, and experimentally titrate the antibody against a single concentration of B, and then measure the % of B that is bound after the solutions reach equilibrium, I ...
- 49
0
votes
2
answers
42
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Detecting multiple compounds on a lateral flow immunoassay – is this a case of monoclonal vs polyclonal antibodies?
I am working on developing lateral flow immunoassays for drugs of abuse and needed some advice on detecting multiple drug compounds in one immunoassay.
As an example, an existing LFIA on the market ...
- 113
3
votes
2
answers
776
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What does it mean to be a fully human monoclonal antibody?
I somewhat understand that some monoclonal antibodies are developed from the cells of mice, or a fusion of human and mice genes.
When something is a fully human monoclonal antibody does that mean it ...
- 283
2
votes
1
answer
10k
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Meaning of units in ELISA based tests?
For some ELISA based antibody tests (e.g. h-tTg antibody test), labs report units as RU/mL or U/mL. Also different labs have different cut off (normal range) values.
I understand that different kit ...
- 139
5
votes
2
answers
117
views
Antibody staining
I am staining tissue sections and I did a mistake, I was supposed to have mixed 3 primary antibodies but I stained only with one of them. After the 1h incubation I washed 10 min with PBS and then I ...
- 237
15
votes
2
answers
5k
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Is antivenom fatal?
Antivenom is used for curing snake bites. Is it fatal when used without being bitten by a snake?
- 167
0
votes
2
answers
58
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Will a polyclonal antibody attach to proteins of different kDA?
If I have a GST 26kDa polyclonal antibody, will it bind to the GST 28kDa protein as well?
2
votes
2
answers
443
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What are the typical values of probabilities for nonspecific binding to an antibody?
I'm physicist by training, so please excuse me if I don't use the proper terminology.
I think there is a way to make a sensor that detects if a single antibody has caught something from the flow. So ...
- 123
4
votes
1
answer
3k
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Dimerization of Immunoglobulin G
I would like to know the specific determinants for formation of IgG dimers. My understanding is the stem of the antibody is a homodimer of two heavy chains, covalently bonded through two disulfide ...
user560
5
votes
2
answers
1k
views
Isotype control antibodies in Flow Cytometry
In a Flow Cytrometry, one can add an Isotype Control Antibody to allow another antibody to bind more specific to the cells.
My question is, how can the Isotype Control Antibody add specificity to the ...
Jeffrey
3
votes
1
answer
184
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Structure of RAP Antibodies (Specifically RAP-5)
[EDIT] - Have just found not one but two papers that address my structure problem. However they concern RAP-1A, so I guess my question is now what is the difference in structure and function of RAP-...
- 41