All Questions
4 questions
1
vote
1
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390
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TMB vs ECL for ELISA: which detection method is more sensitive?
I failed to find a comparison, does anyone know which ELISA detection method is more sensitive of these two: ECL, TMB?
Many thanks!
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0
votes
1
answer
153
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Rationale behind washing off unbound protein antigen in indirect ELISA
My question is with regards to the usability of the indirect ELISA in measuring protein concentration. Suppose we want to quantify the concentration of a protein. The indirect ELISA method would ...
- 163
3
votes
1
answer
284
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About sandwich ELISA
In sandwich ELISA the Fc region of primary antibody bind to the polystyrene coated well. But what are the specific interactions (like 'hydrophobic interaction' or 'van der waals force') happen between ...
- 33
3
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2
answers
120
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Can ELISA be used to detect a plant enzyme? Creating assay for a new enzyme
If the goal is to generate a rapid assay for an enzyme of plant source what are the typical options?
i.e. Could one do something like: Generate an antibody to the enzyme and then use it to create an ...
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