All Questions
Tagged with antibody protein-binding
4 questions
4
votes
1
answer
552
views
How does one derive a KD from an equilibrium titration experiment?
If I have an antibody A and a target B, and experimentally titrate the antibody against a single concentration of B, and then measure the % of B that is bound after the solutions reach equilibrium, I ...
- 49
1
vote
1
answer
1k
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Can multiple antibodies bind the same antigen?
Given the size difference between small molecule antigens and antibodies is it ever possible for multiple antibodies to bind to the same antigen if they recognize different domains on that antigen? ...
- 49
2
votes
0
answers
282
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Does antibody staining / immunolabeling block or inhibit protein function?
In dissociated live cells, does staining them with an antibody block the protein's function?
I am sorting cells based on their expression of a few marker genes, and culturing them post-sort for ...
- 321
2
votes
2
answers
98
views
How to bind antibodies to study their properties?
I want to experimentally look at the behavior of antibodies. To do so, I need to be able to bind these antibodies to a substrate.
Eventually, I want to study the specificity of antibodies to know to ...
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