Questions tagged [bacteriology]

A subdivision of microbiology dedicated to the study of bacteria.

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Will there be less actinobacteria spores in Arizona compared to next to a lake in South Carolina?

Question is in the title. My guess is that there would be less bad bacteria in a home in Arizona than in South Carolina next to a lake. Is that correct? Also, how can one measure the amount of "bad ...
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Bacterial Growth Formula Values

In the equation to calculate the growth rate constant (k), LnN2 = kt + LnN1, where do the values of of N1 and N2 come from? Would they be the colony numbers at time 0 and beginning of log phase?
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official authoritative source for bacterial shape classification

I'm a bit disappointed but after several hours of search, I could not find an official authoritative source for bacterial shape classification. I find a lot, I mean a lot of links but no original ...
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What kind of Phosphate is used for fermentors?

Do biotechnology companies buy SSP, TSP, or some other purer phosphate for bioreactors?
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Bacterial Growth Formula

What are the units of $N$: the colonies of bacteria or the viable cell count? This is in regards to the bacterial growth formula used during serial dilutions, $$ N =N(0) e^{kt} \quad . $$
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Are PVC&PET bacteria found inside homes?

I am interested in weather PVC and PET eating bacteria can be found inside people's homes. The PET consuming ones were found in or near plastic waste dumps or in the ocean, but would they also have ...
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Are most pathogenic bacteria facultative anaerobes?

I know that S. aureus and most gram-negative rods are facultative anaerobes. In terms of number of species, are most human pathogen-associated bacteria facultative anaerobes?
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How many types of bacteria?

Bacteria are classifed on the base of food. Two types of bacteria, autotrophic and heterotrophic bacteria. Autotrophic bacteria that prepare own food or energy. Heterotrophic which cannot prepare own ...
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How do parasites “reprogram” brains?

Dicrocoelium dendriticum is a parasite that uses ants as a second intermediate host (which means that ants aren't its primary host/target, but are rather a temporary host to help reach their main/...
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What disease does Saccharopolyspora erythraea cause?

For an examination assignment I have to find a disease caused by the bacterial species Saccharopolyspora erythraea, but I have searched the internet and have found no report of patients being infected ...
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E. coli & aspartate

I am familiar with the linkage between aspartate/ligand binding, receptor methylation, and flagellar behavior re. style of locamotion, but I do not know what the bacterium does with the aspartate it ...
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51 views

Is bacillus strain 2-9-3 really 250 million year old?

I've read that in the last decade (I believe) some scientists discovered a microorganism, which they dubbed bacillus strain 2-9-3, in a crystal of salt that they think is 250 million years old and ...
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Are there completely self-sufficient autotrophs?

Are there completely self-sufficient autotrophs which can survive and reproduce indefinitely by consuming exclusively non-organic matter (plus energy from sunlight or thermal vents etc.)? I'm asking ...
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Do bacteria exist which live exclusively on non-organic matter?

Are there bacteria which consume exclusively non-organic matter? Meaning that they do not require organic matter from other organisms to stay alive and reproduce? A completely pacifistic bacterium. ...
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How long can Legionella survive at 60°C?

I'm coding some stuff for water heaters and one of the functions is anti-Legionella. Anyway, I've read on wikipedia that 90% Legionella dies in first two minutes if the water temperature is 60°C, but ...
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What enzyme or bacteria could dissolve plastic?

What enzyme or bacteria could dissolve plastic aside from acetone? Preferably, low-cost and environmentally friendly. Any suggestions would be helpful. I'm targeting for thin plastic (low-density ...
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Why is it possible to make yoghourt with lactose free milk?

The function of the Lactobacillus bulgaricus and Streptococcus thermophilus cultures is to ferment lactose to produce lactic acid. The increase in lactic acid decreases pH and causes the milk to clot,...
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Fermenting bacterial growth with potato dextrose broth

I am trying to propagate some strains of bacteria (L.Plantarum, B.Subtilis, L.Rhamnosus, B.Longhum, B.Bifidum) in potato dextrose suspension for my own consumption. I would like some guidance, ...
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Salt Bridge for Microbial Fuel Cell: Full or Partly Full?

I'm making a microbial fuel cell out of benthic mud and salt water in containers. My salt bridge will be made of agar and table salt solidified in PVC pipes. Does the salt bridge connecting the anode (...
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What are the survival conditions for Clostridium botulinum?

I notice that the incidence of poisoning from the activity of Clostridium botulinum is quite rare. For example, in 2015 there were only 7 incidents of foodborne botulism poisoning identified even ...
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Are there any non-pathogenic gram-positive bacteria in human gut?

I wish to know if there are any non-pathogenic (not even opportunistic pathogen) gram-positive bacteria in the human gut? Is there any reference that shows there exists a non-pathogenic gram-positive ...
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Immune response to IgA positive bacteria

If certain bacteria can be coated with IgA in vitro, does that mean they are likely to elicit an IgA immune response? Edit I'm working on a project that involves IgA-Seq analysis. Bacteria are coated ...
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Can you design a bacteriophage that attacks the part of the bacteria that makes them antibiotics resistant?

I read in a newspaper about a case where they had found a virus that could attack a specific form of antibiotics-resistant bacteria and managed to save a girl from a certain death by "infecting her" ...
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what are best methods for mrsa screening in hospital?

methods for screening MRSA are based on PCR (mecA detection) and MRSA latex test and culture. I need to know sensitive, specific and cost-effective methods for diagnosis MRSA infection on one work day ...
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Could bacteria and phages be found in tissues?

in the literature, there are several studies of the gut virome and microbiome, for instance Reyes et al. on Nature (https://www.nature.com/articles/nature09199?error=cookies_not_supported&code=...
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Type of Immunity- Bacterial septicaemia

what type of immunity (Non-specific, Anti-body mediated, cell-mediated) is expressed in Bacterial Septicaemia (Bacterial contamination in systemic circulation)?
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Size distribution of all known species in the domain bacteria

I have found the smallest bacteria are around 200nm (e.g Mycoplasma genitalium an ultramicrobacterium) and largest are around 700um (Thiomargarita namibiensis). I am looking for a graph that ...
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How do I homogenise a small pellet of 10 to 50 cells within less than 50 µL?

I have a problem with declumping/ homogenising bacterial cells in small volumes (10 to 50 µL). I know that I can get all of my cells out of the tube, but they are still stuck together and won't form ...
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How to grow Bacillus subtilis spores for making natto?

I enjoy making fermented foods, one of them is natto, which is cooked soybeans fermented with B. subtilis. I usually buy the B.s. spores online, but they are somewhat expensive and often hard to find....
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What is a secretion target family protein?

I came across this link: https://www.genome.jp/dbget-bin/www_bget?btw:BF38_3398 What is this protein, is it a secreted effector protein or is it something else? What is the difference between type ...
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What is the frequency of AUG near-cognates (as start codon) in ‘Leaderless’ mRNA?

In typical bacteria that use SD-dependent translation initiation, the AUG start codon may sometimes be replaced by near-cognates (GUG, UUG, etc.). The frequency of these near-cognates is somewhat ...
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If a bacterium had a protein on its surface that humans also have, would it cause an autoimmune disease?

Suppose that a bacterium happened to have a protein on its surface. This protein can also be found in the human body. If this bacterium were to then infect a human with an otherwise normal immune ...
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What is the role of lactic acid bacteria (such as L. acidophilus and L. bulgaricus) in glycolysis during yogurt fermentation?

I am trying to prove that an increase in monosaccharides (glucose, fructose, galactose) that have been added to milk during fermentation will result in a decreased pH. My current argument is: "The ...
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How did sneezing evolve?

Did we develop the sneezing mechanism to get rid of pathogens like bacteria and viruses in our nose? Or did pathogens develop a way to make us sneeze to propagate themselves? Or did we develop the ...
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What happens to Bacteria at a low pH?

I am conducting an experiment on how different pH levels effect the growth of E.coli. After researching, it is predicted that the most acidic pH level (pH 2) will be most effective at killing (or at ...
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Is there a glossary about which infrared (IR) colors which body parts reflect?

I am doing medical research and I'm trying to find parts of the body that look about the same to the human eye, but reflect different "colors" in IR, so that using a light source and a camera of that ...
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How does the symbiotic bacteria reaches a human caecum

My thought on this matter is that we have got a very high pH of about 1.8 in our stomach.So,most of bacteria who came in through food or just open mouth should be killed by it.So,how does a bacterial ...
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What happens to Bacterial Cells stored at 4 degrees?

4C is when water is most dense and is not so low as to cause ice crystal formation. For short term storage bacterial cultures are often simply kept in a fridge at 4C. I guess that this significantly ...
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Thermophilic Total plate count with PCA with Triphenyl tetrazoliumchloride as supplement

Hello I'm am a fourth year microbiology analyst and I'm busy with finishing my final internship. During my final internship I have to complete a own assignment. the assignment I got was to find out if ...
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Loss of antibiotic resistance due to long term storage

How possible is it for antibiotic resistant bacteria stored for a long time to lose such resistance?
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How reliable is the life cycle information on PhagesDB?

If I'm understanding this correctly, PhagesDB users choose the Cluster/Subcluster during the upload, and the life cycle type is assigned solely based on this data. How reliable is that information? ...
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Killing microorganisms on agar plates?

If I grow microorganisms on agar plates and I expose them to UV light. How exactly will I know that the microorganisms have been killed? I was watching a video that showed spots in the petri dish ...
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Could bacteria theoretically evolve to survive in space? What is the maximum altitude in the atmosphere that anaerobic bacteria can survive?

There is no edge to the atmosphere, and therefor I expect there would be a gradual reduction of concentrations of bacteria as we ascend up into space. Logically anaerobic bacteria should be able to ...
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Home bacteria culture at home?

Can simple bacterial culture be done at home using food agar as the medium (with some additives found in the store)?
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E. Coli growth inhibited in Powdered Honey

I ran into problem with my method validation since the E. Coli that I've spiked into my Honey Powder sample can't grow in the media. The media I've used is Merck CCA (Chromogenic Coliform Agar). I've ...
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Are the bacteria doing the same photosynthesis as plants do?

I mean Plants during photosynthesis produce oxygen but Do bacteria also produce oxygen during photosynthesis or produce other gases? If yes , how do these bacteria process photosynthesis by ...
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Why is there such in interest in antibiotics against Pseudomonas?

I keep finding information on anti-pseudomonal antibiotics, or information specifying the antibiotic has anti-pseudomonal activity. Is there anything peculiar to this bacterium when compared with ...
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How can i count my E. coli colonies?

I did a test which involved growing e. coli on a petri dish. I did not dilute the sample, and this resulted in growing hundreds of small, overlapping colonies. However, they still can be counted, but ...
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Why do we have an immune system?

My daughter asked a question that I could not answer. If you have a cold, you have symptoms such as fever, cold and sore throat. These symptoms, however, are the reactions of the immune system to ...
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What happens when the bacteria in the petri dish lack nutrients for long time?

If I put some E.coli in a petri dish filled with limited nutrition in room temperature and forget them for months or years. What will happen in the final after they overgrowth and used all nutrition ...