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Questions tagged [bacteriology]

A subdivision of microbiology dedicated to the study of bacteria.

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How do I homogenise a small pellet of 10 to 50 cells within less than 50 µL?

I have a problem with declumping/ homogenising bacterial cells in small volumes (10 to 50 µL). I know that I can get all of my cells out of the tube, but they are still stuck together and won't form ...
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How to grow Bacillus subtilis spores for making natto?

I enjoy making fermented foods, one of them is natto, which is cooked soybeans fermented with B. subtilis. I usually buy the B.s. spores online, but they are somewhat expensive and often hard to find....
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What is a secretion target family protein?

I came across this link: https://www.genome.jp/dbget-bin/www_bget?btw:BF38_3398 What is this protein, is it a secreted effector protein or is it something else? What is the difference between type ...
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E. coli (K-12) sample is not dying to isopropyl alcohol

I am doing a high school science project on the effectiveness of household antiseptics and the resistances the bacteria evolve to them. I am using E. coli K-12. In the sample, the bacteria population ...
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What is the frequency of AUG near-cognates (as start codon) in ‘Leaderless’ mRNA?

In typical bacteria that use SD-dependent translation initiation, the AUG start codon may sometimes be replaced by near-cognates (GUG, UUG, etc.). The frequency of these near-cognates is somewhat ...
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83 views

If a bacterium had a protein on its surface that humans also have, would it cause an autoimmune disease?

Suppose that a bacterium happened to have a protein on its surface. This protein can also be found in the human body. If this bacterium were to then infect a human with an otherwise normal immune ...
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What is the role of lactic acid bacteria (such as L. acidophilus and L. bulgaricus) in glycolysis during yogurt fermentation?

I am trying to prove that an increase in monosaccharides (glucose, fructose, galactose) that have been added to milk during fermentation will result in a decreased pH. My current argument is: "The ...
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1answer
45 views

How did sneezing evolve?

Did we develop the sneezing mechanism to get rid of pathogens like bacteria and viruses in our nose? Or did pathogens develop a way to make us sneeze to propagate themselves? Or did we develop the ...
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1answer
45 views

What happens to Bacteria at a low pH?

I am conducting an experiment on how different pH levels effect the growth of E.coli. After researching, it is predicted that the most acidic pH level (pH 2) will be most effective at killing (or at ...
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22 views

Is there a glossary about which infrared (IR) colors which body parts reflect?

I am doing medical research and I'm trying to find parts of the body that look about the same to the human eye, but reflect different "colors" in IR, so that using a light source and a camera of that ...
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45 views

How does the symbiotic bacteria reaches a human caecum

My thought on this matter is that we have got a very high pH of about 1.8 in our stomach.So,most of bacteria who came in through food or just open mouth should be killed by it.So,how does a bacterial ...
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29 views

What happens to Bacterial Cells stored at 4 degrees?

4C is when water is most dense and is not so low as to cause ice crystal formation. For short term storage bacterial cultures are often simply kept in a fridge at 4C. I guess that this significantly ...
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Thermophilic Total plate count with PCA with Triphenyl tetrazoliumchloride as supplement

Hello I'm am a fourth year microbiology analyst and I'm busy with finishing my final internship. During my final internship I have to complete a own assignment. the assignment I got was to find out if ...
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1answer
68 views

Loss of antibiotic resistance due to long term storage

How possible is it for antibiotic resistant bacteria stored for a long time to lose such resistance?
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23 views

How reliable is the life cycle information on PhagesDB?

If I'm understanding this correctly, PhagesDB users choose the Cluster/Subcluster during the upload, and the life cycle type is assigned solely based on this data. How reliable is that information? ...
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1answer
99 views

Killing microorganisms on agar plates?

If I grow microorganisms on agar plates and I expose them to UV light. How exactly will I know that the microorganisms have been killed? I was watching a video that showed spots in the petri dish ...
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Could bacteria theoretically evolve to survive in space? What is the maximum altitude in the atmosphere that anaerobic bacteria can survive?

There is no edge to the atmosphere, and therefor I expect there would be a gradual reduction of concentrations of bacteria as we ascend up into space. Logically anaerobic bacteria should be able to ...
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1answer
33 views

Home bacteria culture at home?

Can simple bacterial culture be done at home using food agar as the medium (with some additives found in the store)?
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1answer
25 views

E. Coli growth inhibited in Powdered Honey

I ran into problem with my method validation since the E. Coli that I've spiked into my Honey Powder sample can't grow in the media. The media I've used is Merck CCA (Chromogenic Coliform Agar). I've ...
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1answer
50 views

Are the bacteria doing the same photosynthesis as plants do?

I mean Plants during photosynthesis produce oxygen but Do bacteria also produce oxygen during photosynthesis or produce other gases? If yes , how do these bacteria process photosynthesis by ...
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1answer
42 views

Why is there such in interest in antibiotics against Pseudomonas?

I keep finding information on anti-pseudomonal antibiotics, or information specifying the antibiotic has anti-pseudomonal activity. Is there anything peculiar to this bacterium when compared with ...
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1answer
40 views

How can i count my E. coli colonies?

I did a test which involved growing e. coli on a petri dish. I did not dilute the sample, and this resulted in growing hundreds of small, overlapping colonies. However, they still can be counted, but ...
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1answer
66 views

Why do we have an immune system?

My daughter asked a question that I could not answer. If you have a cold, you have symptoms such as fever, cold and sore throat. These symptoms, however, are the reactions of the immune system to ...
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1answer
36 views

What happens when the bacteria in the petri dish lack nutrients for long time?

If I put some E.coli in a petri dish filled with limited nutrition in room temperature and forget them for months or years. What will happen in the final after they overgrowth and used all nutrition ...
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1answer
41 views

What is the difference between transport proteins and effector proteins in gram-negative bacteria?

When considering pathogenic gram-negative bacteria, is there any difference between the function of transport proteins and effector proteins? Or are they of the same functionality? Any reference ...
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29 views

Can a plastic degrading bacteria project be recreated in a school lab environment?

I am looking for a science fair project and am wondering if this could be a good idea. I am in the 10th grade. I am not exactly sure of the materials needed since this is just the beginning stages of ...
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Can a plastic degrading bacteria science fair project be recreated in a school lab?

i am just looking for a science fair project and am fascinated by this topic. i was wondering if a project concerning the isolation of plastic degrading bacteria could be done in a school lab. I am in ...
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3answers
231 views

can a bacterium cell become a cancer?

I don't mean if a bacterium can be the cause of cancer inside a human. But can actually a bacterium changes in the way as normal cells change into tumor cells? So gaining such characteristics of a ...
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1answer
64 views

Psychrotrophic Bacteria

I want to know what gene or protein makes bacteria psychrotrophic, as opposed to regular bacteria that prefers warmer conditions. If you don’t know exactly, and you have suggestions as to where I can ...
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3answers
161 views

A Hadeeth on the fly

I think most biologists here on this site will not consider my question below to be unfit for posting on Biology SE. I came across this article about the hadeeth (a hadeeth is a saying of the ...
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0answers
1k views

What is the difference between enrichment media and selective media?

Enrichment media These media are used to suppress commensal bacteria while allowing the pathogen to remain viable and grow. [Source: Ananthnarayan and Paniker's textbook of Microbiology.] ...
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1answer
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Searching a fructose degrading bacteria [closed]

my question is do you know a fructose degrading bacteria? Wich is straitforward to use and cultivate? Wich shouldn't create excrements, that can't be removed simple.
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1answer
73 views

How does anaerobic bacteria produce ATP for it's own cellular activities?

Anaerobic bacteria utilise glycolysis: Glucose + 2 P + 2 NAD+ => 2 ATP + 2 H + 2 NADH + 2 H2O + 2 Pyruvate followed by fermentation: Pyruvate + NADH => Lactate + NAD+ The resultant NAD+ formed ...
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What causes bacteria to produce a mixture of L-lactic acid and D-lactic acid?

Lactic acid is an organic compound with the formula CH3CH(OH)COOH. Lactic acid is chiral, consisting of two optical isomers. One is known as L-(+)-lactic acid or (S)-lactic acid and the other, its ...
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How do the bacteria producing bacteriocins protect themselves from it?

Whenever there is Col plasmid in a bacteria it will produce bacteriocins which will kill other bacteria around it. Why will the bacteriocin not kill the bacteria which has produced it? How does the ...
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1answer
85 views

Why reaginic antibodies are absent in these types of syphilis?

According to Textbook of Microbiology and Immunology 2e, Subhash Chandra Parija, pg.no; 375 These(reaginic) antibodies do not appear in early primary syphilis, latent acquired syphilis of long ...
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2answers
111 views

How to grow bacteria?

We have this science experiment on how to grow a bacteria, our teacher told us to use gelatin, beef stock, and sugar as an alternative for agar. I am confused on why we need gelatin, beef stock, and ...
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1answer
39 views

Vibrio vulnificus from liquid soap

At some point between 2002 and 2006 I attended a departmental seminar in the UK on Vibrio infections, and particularly on the (then relatively unheard of) V. vulnificus, which causes necrotizing wound ...
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4answers
171 views

Why aren't all infections immune-system resistant?

It's been less than a century since the widespread use of antibotics started, and already we're seeing bacteria that have evolved immunities to the antibotics we use. On the other hand, we've been ...
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3answers
81 views

Is it possible to prove that a hand sanitizer can kill 100% of a person's germs?

We have a project for Economics, and we need to advertise something that consumers will be willing to buy. My group thought of "improved" hand sanitizer. So, instead of it killing 99.9% germs, we ...
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0answers
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Can the human gut work with other type of gut bacteria?

I was wondering if the human body would be capable of functioning with, say, cellulolytic bacteria in their gut (instead of or in addition of their current bacteria), like that of ruminants and if ...
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2answers
111 views

why don't antibiotics reduce our weight?

I know that a significant weight of the human body (in Kgs) is contributed by microbiota. I also know that antibiotics can often be broad spectrum and kill all the bacterias regardless of them being ...
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1answer
58 views

Why is AMR not widespread?

we know that resistant strains (MDR, XDR, PDR) have arisen in single incidents around the globe but why are these bacteria not common and not spreading quickly as humans keep using antibiotics and ...
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1answer
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Technique to test antibiotic resistance in a bacteria in presence of another compound

so I have to 'quantitatively' assess a bacterial strain for its antibiotic resistance property (it is a specific antibiotic) in the presence and absence of another compound alongside antibiotic. What ...
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2answers
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Is it true that it is mostly impossible, for any kind of antibiotic, to succeed in killing off every bacteria in a very rich environment?

It is commonly believed that the resistance to antibiotics by micro-organisms is truly evolution at work, and that the recent surge in superbugs may very well be attributed to it. When we refer to a ...
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369 views

largest and smallest gram positive, gram negative and fungal pathogens

As I stumbled through this question: A 16-year-old female patient presented to her family physician with a complaint of an abnormal vaginal discharge and pruritus (itching). The patient ...
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1answer
156 views

Can E. coli make pyruvate from acetate?

What pathway would E. coli use to make pyruvate from acetate? I have found several papers that refer to a possible mechanism that could move acetate to pyruvate via a three-step process: Step 1: ...
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1answer
625 views

Why do bacteria use formylated methionine in the initiator tRNA, while eukaryotes do not?

Could anyone suggest an explanation for the evolution of this trait in bacteria? Does it confer any advantage? It is also exploited by immunity receptors of some eukaryotes for the recognition of ...
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1answer
37 views

Spoiled 1% milk

A friend of mine gave me a jug of 1% milk and when I got home to put it in my fridge I noticed that it was already out of date so I set it on the counter next to our trash can and at the time we didn'...
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1answer
249 views

Heat shock vs electroporation

I've been transforming E. coli via heat shock in order to insert oligonucleotides (around 50 nt); however, none of my experiments have given positive results so far. I begin to question the efficiency ...