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Questions tagged [bacteriology]

A subdivision of microbiology dedicated to the study of bacteria.

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How do I grow 5 liters of purple non-sulfur bacteria?

I am running experiments for a project that requires large amounts of purple non-sulfur, Cyanobacteria and nitrogen fixing bacteria. I plan on growing all the species in separate tanks. The species' I ...
Aakarsh Tathachar's user avatar
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What is a reference strain in DNA-DNA hybridization DNA groups?

Results DNA groups identified: All intra-group relatedness values are shown in Table I. By means of reference strains, most of the DNA groups could be identified as groups described by Bouvet & ...
Freezing Soul's user avatar
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Dataset of bacterial growth according to time from an experiment with\without antibiotics [closed]

I'm looking for a dataset about the growth of a bacteria according to time if possible the name of the bacteria and the condition (the protocol). Also if possible I would like to have this sort of ...
diplodocass's user avatar
5 votes
1 answer
300 views

Where does Actino- come from in the genus Actinomycetota?

This comment under Bacteria trapped in crystal inclusions found 'alive' after 50,000 years - what were they eating all that time? links to Quintana, Badillo ann Maldonado (2013) ...
uhoh's user avatar
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What is the largest culturable bacterium?

E. coli is easy to grow in the lab. It has a volume around $0.6 \mu m^3$. Source T. namibiensis can be much larger, with a volume on the order of ${10^6}\mu m^3$. Source However, T. namibiensis is not ...
julianstanley's user avatar
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Does heterogeneous resistance have an impact on the zone diameter observed in disk diffusion susceptibility testing?

Question Does the presence of small, discrete colonies within the zone of inhibition, suggesting heterogeneous resistance, impact the zone diameter in disk diffusion susceptibility testing? "Help ...
Freezing Soul's user avatar
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Can bacterial DNA solutions, which include other cellular components, be stored without compromising DNA stability?"

Question Can bacterial DNA solutions, which include other cellular components, be stored without compromising DNA stability?" Details I'm using the boiling extraction method to extract ...
Freezing Soul's user avatar
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2 answers
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How is the zone of inhibition formed in disk diffusion?

Question How is the zone of inhibition formed in disk diffusion? What I mean by the formation of the zone of inhibition is not the ultimate visibility of such zone but the circumstances that result in ...
Freezing Soul's user avatar
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What's pooling in the spread plate technique?

"Distributing the organisms by rotating the spreader rather than the plate tends to cause more pooling of the inoculum." https://asm.org/ASM/media/Protocol-Images/Preparing-Spread-Plates-...
Freezing Soul's user avatar
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Does inoculum volume affect spread plate outcome?

Background The spread plate method is a technique used in microbiology to enumerate and isolate bacteria or other microorganisms present in a sample. It involves evenly spreading a liquid sample ...
Freezing Soul's user avatar
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Did operon evolve after endosymbiosis and specialisation of mitochondria?

We know that all extant bacteria use the operon system as their mode of regulating gene expression, in contrast to eukaryotes, which use individual gene promoters as well as epigenetic mechanisms, ...
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Sourcing sulfur-oxidizing bacteria

For a research project, I need a species of sulfur-oxidizing bacteria but I cannot find a biological company that sells anything like that. I know ATCC sells some species of sulfur-oxidizing bacteria ...
Aakarsh Tathachar's user avatar
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Can placing a culture plate upright introduce contaminants from the lid?

Discussion I have developed a habit of keeping culture plates always inverted, especially those of non-selective media. This precaution is followed after the poured plate is placed inverted in the ...
Freezing Soul's user avatar
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Why did a resazurin broth bottle turn pink immediately after being inoculated?

I have lawn cultures of aerobic bacteria kept at (2-8 °C), which I am trying to revive since they were kept longer than recommended. First, I tried to incubate a broth culture inoculated with a ...
Freezing Soul's user avatar
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Is there a way to isolate bacteria from a bacteria/phage plaque in order to PCR bacteria only?

I am writing a paper for a class that involves a mock grant proposal. I am looking at an experiment which requires sequencing of host DNA from a virus/host model, and am having a hard time finding a ...
Erin Marble's user avatar
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Can Gravity convection incubators negatively affect the growth of strict aerobic bacteria?

Background Gravity incubators rely on natural convection for heat distribution. There is no fan or blower system, and the heat rises from the heating element at the bottom of the incubator. https://...
Freezing Soul's user avatar
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Can a gene transfer from a plasmid to a bacterial chromosome?

Plasmids are involved in providing resistance to antibiotics. So, can that gene or any other gene transfer from the plasmid to the bacterial chromosome? Is there any chance where antibiotic resistance ...
Amit Kumar's user avatar
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How can Clostridium tetani proliferate in relatively minor wounds?

It is often reported (NIH) that some of the most common infections by Clostridium tetani are in minor wounds where, in theory, blood (hence oxygen) supply should not be completely disrupted. How can ...
AlexanderCar's user avatar
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Storage of bacteria in MgCl2 at 4 degrees

I have a small question to which I am unable to find a proper answer. Will bacteria in a MgCl2 buffer (or similar solution) at +4 degrees Celsius survive storage for 1-2 days? Or will they die? What ...
Dunois's user avatar
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How can one store non-sterile culture media in dissolved form in the refrigerator?

How do I store non-sterile culture media in dissolved form in the fridge in a clean but non-sterile container? The powder of the media (Broth, Nutrient agar, MAC, or MH agar) will be put in a glass ...
Freezing Soul's user avatar
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1 answer
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How do i create sustainable readily available stock cultures to act as Gram stain controls?

My investigation on the matter Ref #1 Staining known Gram-positive and Gram-negative organisms on either side of your unknown organism act as positive controls for your technique. (Microbiology ...
Freezing Soul's user avatar
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1 answer
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Can a non-pure culture be used for reliable rapid phenotypic diagnostic tests?

Can a non-pure culture be used for reliable rapid phenotypic diagnostic tests (e.g. catalase, oxidase & gram stain)? "Slide Test Method Obtain a pure culture of the organism to be tested. ...
Freezing Soul's user avatar
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Ames Assay Confusion: Aren't the odds of spontaneous revertants too low to be able to accurately test the mutagenicity of certain compounds?

I am a student conducting a test with the Ames Assay. This assay uses a strain of bacteria that has a mutation in an amino acid synthesizing operon, which doesn't allow it to synthesize its protein. ...
Kyotiq's user avatar
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3 votes
1 answer
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Why are my bacterial smears disappearing?

I'm trying to inspect simple stained bacterial smears. But my smear suddenly disappears after a successful inspection with the oil immersion lenses. The background can become too red (the color of the ...
Freezing Soul's user avatar
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What could be the cause of strange brown discoloration around some bacterial strains' colonies on macconkey agar?

Acinetobacter Unknown (Yellow TSI, Oxidase negative, Catalase positive) Unseeded plate My only guess is that the initial pH of the poured agar is too acidic (too red), so any alkalinity is more ...
Freezing Soul's user avatar
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1 answer
159 views

How do microbiologists decide whether two morphotypes on the same plate belong to the same strain?

Background Colony: A colony, in microbiology, is a visible cluster or mass of microorganisms (such as bacteria or fungi) that has grown and multiplied on a solid agar medium. Each colony represents a ...
Freezing Soul's user avatar
1 vote
1 answer
151 views

Is there a reliable method to determine if heat fixation was inadequate before proceeding with further staining steps?

Background Heat Fixation: Heat fixation is a technique used in bacteriology and other biological sciences to affix bacterial cells or other microorganisms to a microscope slide. This is achieved by ...
Freezing Soul's user avatar
1 vote
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Carbon dioxide concentration in alkaline media

I am running a batch experiment using penicillin bottles where I have bacteria growing for 21 days in a highly alkaline media (pH 10). I am measuring the CO2 and O2 content in the headspace over time, ...
Franco Grosso's user avatar
1 vote
1 answer
99 views

Does cooking kill all bacteria?

So I know that the general rules is that pathogen prone foods such as chicken shouldn't be consumed after 2 hours at room temperature. Cooking meat such as chicken is necesary as it kills the ...
Ethan's user avatar
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How do the qualitative tests for E.Coli work, which chemicals are used?

There are test kits that to detect E. Coli in water, by mixing a small sample of water with a certain chemical. After a certain time, the sample changes color depending on the presence of E. Voli ...
mart's user avatar
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How can one use motility testing as a practically reliable test for organisms identification in bacteriology?

Background information: The motility test is used to determine whether an organism is motile or non-motile. There are three types of bacterial motility *: Active movement: true motility that we are ...
Freezing Soul's user avatar
1 vote
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Can single-Use Gloves carry bacteria on their outer surface?

In bacteriology, can I touch items (e.g. oxidase discs) with my gloves not fearing to contaminate these items? Are these gloves (e.g. Disposable nitrile gloves) made so that bacteria can't adhere to ...
Freezing Soul's user avatar
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What's the grade of D-glucose that can be used in glucose assimilation test in bacteriology?

Background information Carbon assimilation test is based on the ability of a bacterial species to utilize an organic compound (e.g. D-glucose) as a sole carbon source. This means that for carbon ...
Freezing Soul's user avatar
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1 answer
57 views

Can I have LAMP on the bench / in the field?

I am interested in LAMP for detecting small amounts of DNA (loop-mediated isothermal amplification and yes, I know the initials don't match). I am trying to figure out exactly how clean/(sterile?) ...
Laura's user avatar
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What is the mechanism behind antibiotic resistance and how can it be mitigated or prevented? [closed]

What causes antibiotic resistance, and how can we combat it? I am interested in the mechanisms behind antibiotic resistance and strategies to prevent it in bacteria.
nane albert's user avatar
1 vote
1 answer
57 views

A DNA extraction protocol that is equipment-less for bacterial metagenomes?

I am going into the field soon to sample a very remote environment. For reasons that are unimportant here, I would like to extract bacterial DNA from these samples in the field. I will have access to ...
donkey's user avatar
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1 vote
1 answer
5k views

Why do bacteria in a urinary tract infection (UTI) cause bleeding?

It seems that when a urinary tract infection happens, bacteria will cause inflammation to the lining of the urinary tract, and this will cause bleeding. What is the exact process for the inflammation ...
flen's user avatar
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Endospores contact with surfaces

I am currently studying endospores-forming bacteria. To be specific B.subtilis. I noticed that the formed spores have strong contact "grip" with the surface it is forming on. For example, ...
Anwar Elhadad's user avatar
5 votes
1 answer
99 views

Why isn't bacterial colony a monolayer?

I am an engineer entering the field of microbiology. I was watching bacterial colonies (lab strain E. coli to be specific) being grown on agar plates. I was surprised that the bacterial colony was not ...
Deep's user avatar
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2 votes
1 answer
48 views

How can a layperson get their hands on a specific strain that's not readily available commercially?

I'm mostly interested to get my hands on L.plantarum D-103, but there are a few other strains as well. D-103 is e.g. mentioned here https://meridian.allenpress.com/jfp/article/80/10/1682/199965/...
Anton Rodenhauser's user avatar
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1 answer
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How to figure out growth/culture conditions for a specific strain?

Specifically, I'd like to find out how to grow ME-3 L.Fermentum (DSM 14241) at home without any fancy equipment. What food does it need? Sugar? Lactose? Something else? At what temperature does it ...
Anton Rodenhauser's user avatar
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55 views

Methods of denaturing BL21 E.coli other than using lysis buffer and sonication

The goal is to try different denaturing methods for BL21 E.coli. However, finding detailed paper on this is challenging as most articles do not seem to include the details of their denaturing ...
Pumla 's user avatar
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Superbugs in eusocial animals

I am not a biologist, and I'm interested in eusocial animals, and the porous habitats in which they live. I know few facts about diseases in these animals: I know some interesting instances of viruses,...
user71674's user avatar
2 votes
1 answer
45 views

What is the easiest way to determine the species of non-tuberculous mycobacteria?

We have isolated a mycobacterium strain from patient sputum using an NTM Elite plate. When inoculated into an MGIT tube, we observe fluorescence when observed under UV light, which means it is highly ...
Johan Wikström's user avatar
5 votes
1 answer
103 views

What's the purpose of using more than one culture medium for bacterial isolation?

What's the purpose of using more than culture medium for bacterial isolation? Why not use just one? If this is not generally used in isolation of various types of bacteria, I'm concerned especially ...
Freezing Soul's user avatar
1 vote
0 answers
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How are spacer sequences created in a prokaryotic genome?

The CRISPR/Cas defense mechanism uses spacer sequences between palindromic repeats to search for the sequence to cut by an endonuclease. But how are these spacers created? Let's take Bacteriophages, ...
Void's user avatar
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7 votes
1 answer
426 views

Has there been evidence that there has been coevolution in E. Coli strains with the T4 bacteriophage?

According to Evolution of T4-related phages, there have been multiple instances of evolution in the T4 coliphages. Per the article, there are many T-even phages that have insertions from other ...
spaik's user avatar
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4 votes
1 answer
551 views

Doesn't pasteurization kill gut bacteria such as Akkermansia muciniphila?

I've recently watched two presentations on YouTube (this and this) about Akkermansia municiphila, a commensal bacteria that feeds on the mucus of the gut. What I find confusing is their claim about ...
Paul Razvan Berg's user avatar
1 vote
1 answer
91 views

Naming the Rings of Flagellar Structure

I will apologise ahead of my very basic question. I just read about Flagella Structure and learned that there are 4 rings, namely "L Ring", "P Ring", "MS Ring", "C ...
green onion's user avatar
5 votes
1 answer
760 views

Why does adding calcium chloride aid in bacteriophage infection?

We are attempting to isolate bacteriophages from environmental soil samples and most protocols recommend adding CaCl2 to the media to aid bacteriophage infection, even though they all recommend ...
Johan Wikström's user avatar

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