Questions tagged [bacteriology]
A subdivision of microbiology dedicated to the study of bacteria.
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What determines if a bacteria has an F-plasmid? Are F-plasmids the only plasmids shared between bacteria?
If I’m designing an expierment to study bacterial concergation (while adding a plasmid of my choice to the bacteria), how do I know the bacteria I’m using has an F-plasmid (or can transfer my ...
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What happens to Bacteria at a low pH?
I am conducting an experiment on how different pH levels effect the growth of E.coli. After researching, it is predicted that the most acidic pH level (pH 2) will be most effective at killing (or at ...
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Use of bacteriophages against viral infections [on hold]
I know that bacteriophages kill cell by essentially blowing up, and I also know that viruses are difficult to treat because they aren’t necessarily alive. Could bacteriophages be modified to attack ...
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Is there a glossary about which infrared (IR) colors which body parts reflect?
I am doing medical research and I'm trying to find parts of the body that look about the same to the human eye, but reflect different "colors" in IR, so that using a light source and a camera of that ...
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How does the symbiotic bacteria reaches a human caecum
My thought on this matter is that we have got a very high pH of about 1.8 in our stomach.So,most of bacteria who came in through food or just open mouth should be killed by it.So,how does a bacterial ...
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What happens to Bacterial Cells stored at 4 degrees?
4C is when water is most dense and is not so low as to cause ice crystal formation.
For short term storage bacterial cultures are often simply kept in a fridge at 4C. I guess that this significantly ...
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Thermophilic Total plate count with PCA with Triphenyl tetrazoliumchloride as supplement
Hello I'm am a fourth year microbiology analyst and I'm busy with finishing my final internship. During my final internship I have to complete a own assignment. the assignment I got was to find out if ...
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Loss of antibiotic resistance due to long term storage
How possible is it for antibiotic resistant bacteria stored for a long time to lose such resistance?
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How reliable is the life cycle information on PhagesDB?
If I'm understanding this correctly, PhagesDB users choose the Cluster/Subcluster during the upload, and the life cycle type is assigned solely based on this data. How reliable is that information? ...
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1answer
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Killing microorganisms on agar plates?
If I grow microorganisms on agar plates and I expose them to UV light. How exactly will I know that the microorganisms have been killed? I was watching a video that showed spots in the petri dish ...
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Could bacteria theoretically evolve to survive in space? What is the maximum altitude in the atmosphere that anaerobic bacteria can survive?
There is no edge to the atmosphere, and therefor I expect there would be a gradual reduction of concentrations of bacteria as we ascend up into space. Logically anaerobic bacteria should be able to ...
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Home bacteria culture at home?
Can simple bacterial culture be done at home using food agar as the medium (with some additives found in the store)?
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1answer
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E. Coli growth inhibited in Powdered Honey
I ran into problem with my method validation since the E. Coli that I've spiked into my Honey Powder sample can't grow in the media. The media I've used is Merck CCA (Chromogenic Coliform Agar). I've ...
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1answer
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Are the bacteria doing the same photosynthesis as plants do?
I mean Plants during photosynthesis produce oxygen but Do bacteria also produce oxygen during photosynthesis or produce other gases?
If yes , how do these bacteria process photosynthesis by ...
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1answer
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Why is there such in interest in antibiotics against Pseudomonas?
I keep finding information on anti-pseudomonal antibiotics, or information specifying the antibiotic has anti-pseudomonal activity. Is there anything peculiar to this bacterium when compared with ...
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1answer
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How can i count my E. coli colonies?
I did a test which involved growing e. coli on a petri dish. I did not dilute the sample, and this resulted in growing hundreds of small, overlapping colonies. However, they still can be counted, but ...
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Why do we have an immune system?
My daughter asked a question that I could not answer.
If you have a cold, you have symptoms such as fever, cold and sore throat. These symptoms, however, are the reactions of the immune system to ...
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1answer
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What happens when the bacteria in the petri dish lack nutrients for long time?
If I put some E.coli in a petri dish filled with limited nutrition in room temperature and forget them for months or years. What will happen in the final after they overgrowth and used all nutrition ...
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What is the difference between transport proteins and effector proteins in gram-negative bacteria?
When considering pathogenic gram-negative bacteria, is there any difference between the function of transport proteins and effector proteins? Or are they of the same functionality? Any reference ...
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Can a plastic degrading bacteria project be recreated in a school lab environment?
I am looking for a science fair project and am wondering if this could be a good idea. I am in the 10th grade. I am not exactly sure of the materials needed since this is just the beginning stages of ...
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Can a plastic degrading bacteria science fair project be recreated in a school lab?
i am just looking for a science fair project and am fascinated by this topic. i was wondering if a project concerning the isolation of plastic degrading bacteria could be done in a school lab. I am in ...
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3answers
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can a bacterium cell become a cancer?
I don't mean if a bacterium can be the cause of cancer inside a human. But can actually a bacterium changes in the way as normal cells change into tumor cells?
So gaining such characteristics of a ...
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1answer
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Psychrotrophic Bacteria
I want to know what gene or protein makes bacteria psychrotrophic, as opposed to regular bacteria that prefers warmer conditions. If you don’t know exactly, and you have suggestions as to where I can ...
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A Hadeeth on the fly
I think most biologists here on this site will not consider my question below to be unfit for posting on Biology SE.
I came across this article about the hadeeth (a hadeeth is a saying of the ...
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What is the difference between enrichment media and selective media?
Enrichment media
These media are used to suppress commensal bacteria while allowing the pathogen to remain viable and grow.
[Source: Ananthnarayan and Paniker's textbook of Microbiology.]
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1answer
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Searching a fructose degrading bacteria [closed]
my question is do you know a fructose degrading bacteria?
Wich is straitforward to use and cultivate?
Wich shouldn't create excrements, that can't be removed simple.
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How does anaerobic bacteria produce ATP for it's own cellular activities?
Anaerobic bacteria utilise glycolysis:
Glucose + 2 P + 2 NAD+ => 2 ATP + 2 H + 2 NADH + 2 H2O + 2 Pyruvate
followed by fermentation:
Pyruvate + NADH => Lactate + NAD+
The resultant NAD+ formed ...
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What causes bacteria to produce a mixture of L-lactic acid and D-lactic acid?
Lactic acid is an organic compound with the formula CH3CH(OH)COOH. Lactic acid is chiral, consisting of two optical isomers. One is known as L-(+)-lactic acid or (S)-lactic acid and the other, its ...
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How do the bacteria producing bacteriocins protect themselves from it?
Whenever there is Col plasmid in a bacteria it will produce bacteriocins which will kill other bacteria around it. Why will the bacteriocin not kill the bacteria which has produced it? How does the ...
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1answer
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Why reaginic antibodies are absent in these types of syphilis?
According to Textbook of Microbiology and Immunology
2e, Subhash Chandra Parija, pg.no; 375
These(reaginic) antibodies do not appear in early primary syphilis, latent acquired syphilis of long ...
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2answers
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How to grow bacteria?
We have this science experiment on how to grow a bacteria, our teacher told us to use gelatin, beef stock, and sugar as an alternative for agar. I am confused on why we need gelatin, beef stock, and ...
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1answer
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Vibrio vulnificus from liquid soap
At some point between 2002 and 2006 I attended a departmental seminar in the UK on Vibrio infections, and particularly on the (then relatively unheard of) V. vulnificus, which causes necrotizing wound ...
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4answers
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Why aren't all infections immune-system resistant?
It's been less than a century since the widespread use of antibotics started, and already we're seeing bacteria that have evolved immunities to the antibotics we use.
On the other hand, we've been ...
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Is it possible to prove that a hand sanitizer can kill 100% of a person's germs?
We have a project for Economics, and we need to advertise something that consumers will be willing to buy. My group thought of "improved" hand sanitizer. So, instead of it killing 99.9% germs, we ...
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Can the human gut work with other type of gut bacteria?
I was wondering if the human body would be capable of functioning with, say, cellulolytic bacteria in their gut (instead of or in addition of their current bacteria), like that of ruminants and if ...
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why don't antibiotics reduce our weight?
I know that a significant weight of the human body (in Kgs) is contributed by microbiota. I also know that antibiotics can often be broad spectrum and kill all the bacterias regardless of them being ...
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1answer
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Why is AMR not widespread?
we know that resistant strains (MDR, XDR, PDR) have arisen in single incidents around the globe but why are these bacteria not common and not spreading quickly as humans keep using antibiotics and ...
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1answer
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Technique to test antibiotic resistance in a bacteria in presence of another compound
so I have to 'quantitatively' assess a bacterial strain for its antibiotic resistance property (it is a specific antibiotic) in the presence and absence of another compound alongside antibiotic. What ...
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Is it true that it is mostly impossible, for any kind of antibiotic, to succeed in killing off every bacteria in a very rich environment?
It is commonly believed that the resistance to antibiotics by micro-organisms is truly evolution at work, and that the recent surge in superbugs may very well be attributed to it.
When we refer to a ...
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Is it easier or more difficult for bacteria to grow in my water bottle with ice?
I have a water bottle that I use everyday at work. It is a wide mouth 1000ml water bottle. I clean it with soap and hot water about once a week.
Normally I fill it with room temp water, but I was ...
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largest and smallest gram positive, gram negative and fungal pathogens
As I stumbled through this question:
A 16-year-old female patient presented to her family physician
with a complaint of an abnormal vaginal discharge and pruritus
(itching). The patient ...
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1answer
119 views
Can E. coli make pyruvate from acetate?
What pathway would E. coli use to make pyruvate from acetate? I have found several papers that refer to a possible mechanism that could move acetate to pyruvate via a three-step process:
Step 1: ...
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1answer
439 views
Why do bacteria use formylated methionine in the initiator tRNA, while eukaryotes do not?
Could anyone suggest an explanation for the evolution of this trait in bacteria? Does it confer any advantage?
It is also exploited by immunity receptors of some eukaryotes for the recognition of ...
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1answer
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Spoiled 1% milk
A friend of mine gave me a jug of 1% milk and when I got home to put it in my fridge I noticed that it was already out of date so I set it on the counter next to our trash can and at the time we didn'...
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1answer
201 views
Heat shock vs electroporation
I've been transforming E. coli via heat shock in order to insert oligonucleotides (around 50 nt); however, none of my experiments have given positive results so far. I begin to question the efficiency ...
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Which among the seven secretion systems in gram-negative bacteria are pathogenic?
Among the 7 secretion systems of a gram-negative bacteria, do all the systems secrete pathogenic proteins that harm the host into which the protein is secreted? Or is it the type 3, type 4 and type 6 ...
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Measuring gene similarity in two bacteria models
I'm currently working with some computational models of bacteria. The bacterium I am considering is an Ecoli K-12 which has 139 genes. I have several models of the same bacterium with different ...
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2answers
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Why was Achromobacter xerosis removed from the NCBI taxonomy?
The Global Catalogue of Microorganisms lists a bacterium called Achromobacter xerosis which is mentioned in several papers and patents. It once existed in the NCBI taxonomy database, with ID 216898. ...
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Why is there an increase of diagnoses of Lyme disease?
On the Center for Disease Control page for Lyme disease, you can see the progression of reported cases of Lyme disease from 2001 until 2016 and see that over time, there are more reported cases of ...
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How does gram-negative bacteria use high molecular weight starch if the membrane is only permeable to small molecular weights?
So one of my question is:
The outer membrane of a gram negative bacterium contains pores that allow only molecules with a molecular weight less than 1,000 to cross.
What does this bacterium need in ...
