Questions tagged [bacteriology]

A subdivision of microbiology dedicated to the study of bacteria.

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Do bacteria select for "soap resistance"?

When people wash their hands with soap, this limits the spread of bacterial infections. That much, I am pretty convinced of. Duh. What I don't get is how this doesn't lead to evolutionary pressure on ...
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How typhoid fever severe case's intestine perforation occur? (non trauma)

In typhoid severe case, intestinal perforation occurs. As stated here [4, 6]. Intestinal perforation is a serious complication of typhoid fever My question here is, how does the bacteria (Salmonella ...
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How to measure the pH of a bacterial species?

I would like to calculate the pH of a certain bacteria species before after an experiment. I was reading about the pH cell of bacteria and I found out about Bacterial Intracellular pH which I ...
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85 views

Mutations in a Petri dish overnight

How long does it take for a bacterial culture in a Petri dish to experience all possible single base pair mutations? Can 12 hours be enough? I want to get an intuition for whether a given mutation is ...
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What is sigma factor PvdS?

I am using MEME suite tool for de novo motif discovery and when I use TomTom to compare my motifs with known motif database, I find that my genes could potentially be upregulated or downregulated by ...
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Is there any alternative to peptone and beef or yeast extract?

I am trying to start a small microbiology project and I need a growth culture for bacteria. I do not want to use the costly nutrient agars you can buy online. I would like to create my own nutrient ...
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Will proper autoclave treatment really "inactivate all resistant bacterial spores"? If not, how widespread are autoclave-resistant bacterial spores?

Wikipedia's says: A widely used method for heat sterilization is the autoclave, sometimes called a converter or steam sterilizer. Autoclaves use steam heated to 121–134 °C (250–273 °F) under ...
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What is the structure of PduK in the Pdu BMC?

My lab is creating 1,2-propanediol utilization bacterial microcompartments (Pdu BMCs) with an operon containing genes for PduA, PduB, PduJ, PduK, PduN, PduU, and PduT. According to Mayer et al., all ...
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Is it known how some heat-resistance Bacillus spores repair their DNA after having been heated to 420 °C?

Background Discussions below several recent posts in Space SE (links below) indicate that bacterial spores are a serious problem when considering how to prevent a future spacecraft mission to ...
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Disposing biohazardous material

I had conducted some disc diffusion tests with E.coli and S.epidermidis. Now, I wanna dispose these petri dishes and was wondering the correct way to do it. Many websites mention adding bleach to the ...
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Why dehydration is linked with heat resistance in bacteria and fungi?

According to wikepedia Dipicolinic acid results in heat resistance of endospore by doing dehdration of the endospore Dipicolinic acid forms a complex with calcium ions within the endospore core. This ...
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How to create long term stock for Bacillus subtilis?

I am trying to create a long-term stock for Bacillus subtilis (Bacterial glycerol stocks). I have looked online for guides and step-by-step tutorials and I found that they don't say where the sample ...
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When Bacillus subtilis form spores vs die?

In my understanding: sporulation in Bacillus subtilis involves the differentiation into two cell types, the endospore, and the mother cell. The endospore coat is a multilayered shell that protects the ...
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Cyanobacteria and the ratio of Iron to Phosphorous

In listening to a recorded lecture that touched on the Great Oxidation Event, a casual remark was made to the effect that the shifting of the Earth's constituents to more availability of phosphorous ...
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Methods for getting clean C. difficile spore preps without spores clumping together

I'm a microbiologist recently who recently started working on an ongoing C. difficile project for the first time. One of the things I need to do is verify the lower detection limit of my qPCR ...
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Does Tryptic Soy Agar (TSA) contain L-cysteine? (Question about Legionella)

I ask because Legionella sp. requires the presence of cysteine. If TSA does contain L-cysteine, is it sufficient to grow Legionella?
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Is there a specific English term for this vessel for microbial sampling?

In Russian, such a container (see pictures) for microbial sampling is called бакпечатка (bakpechatka), as I learned just now. I've no idea about the etimology of the Russian term. Probably "bak&...
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Do E. coli change color (from white/grey to yellow) when they grow older?

In my experiment, I had white colonies initially and they turned yellow. So I thought it´s natural that mature E. coli colonies are yellow and young are white. But it could be that I had two types of ...
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What determines the physical appearance of a tangible amount of bacteria or viruses?

As in the title, I was wondering how would feel an amount of one type of bacteria or virus, big enough to have a tangible size of the sample. What color, state (liquid, solid), textures, smell ecc. of ...
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What are the causes of gene amplification in archaea?

I was studying this article about Genome Sequencing of a Genetically Tractable Pyrococcus furiosus Strain Reveals a Highly Dynamic Genome in order to try to extrapolate some features that could ...
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Co-culture models of bacterial invasion: where to find them?

I am interested in models showing how pathogenic bacteria (namely Escherichia coli O157) can overcome commensal species (thus, causing a disease). This is a basic concept in biology but I can't find a ...
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Novel bacterial strains of bacteria first isolated on the International Space Station, did the space environment lead to these genetic changes?

Question Methylobacterium ajmalii sp. nov., Isolated From the International Space Station (Bijlani et al. Frontiers in Microbiology, 12, p. 534, 2021) is a thorough analysis of "novel strains&...
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In what stage of bacterial growth can penicillin inhibit the growth of the cellular wall?

The bacterial growth has 3 main stages: lag-phase, log-phase and stationary phase. I was wondering in which one of them penicillin can inhibit the growth of the cellular wall of bacteria and why. I ...
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What is this species of bacteria?

Can I ask what is the species of this bacteria? A comment there says that it is probably E. coli. Disclaimer: I don't have an idea about the given video of bacteria as we are asked to solve for the ...
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Bacterial endosymbiosis of bacteria

Endosymbiosis was crucial to the development of complex life and it continues to happen to this day. The origin of mitochondria see bacterial guests in archaea hosts, some of which later took in ...
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Alternative dyes for Gram staining

In the Gram stain, is there any replacement for primary stain, secondary stain, decolourizer and mordant? What results will the replacements produce? I found that crystal violet can be replaced with ...
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Calcium ions and bacterial transformation

What is the mechanism by which calcium ions and heat treatment allow the bacterial membrane to become permeable, allowing the uptake of plasmids?
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Are there phage-eating bacteria?

If phages (bacterium-eating viruses) prowl on bacteria, are there bacteria (or other micro-organisms) that hunt phages for food? They are rich in proteins anyway... Are there studies on this subject? ...
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What fraction of bacterial proteins are membrane bound?

Almen et al. showed that around 27% of the human coding proteins are membrane bound. How does this compare with bacteria such as E.coli or B.subtilis (or any other bacteria)? Is there a paper out ...
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Skeletisation in space

A space pod containing an astronaut drifts out to space and cannot be rescued. The space pod contains no insects, and the only bacteria present are those originally brought in by the astronaut. There ...
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Will wiping a phone down with isopropyl alcohol daily breed bacteria resistant to disinfectants?

The title says it all. If you disinfect something more regularly, aren't you just selecting for bacteria which are most robust to that method of disinfection? I've heard it said about some medications ...
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Does probiotics bacteria from yogurt live in the mouth too? Does it populate the mouth/throat or just the gut?

Yes I was basically just wondered this as I've taken probiotics in a pill form but those only go to the gut. I guessed the mouth needs probiotics too and I wondered if yogurt can accomplish that. I ...
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How does lactate produced in the intestines get transported to the rest of the body?

Some gut bacteria known as lactic acid bacteria(LAB) are known to produce lactate in the intestines and they confer beneficial effects such as enhancement of the immune system, but I assume not all of ...
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Nucleotide Sequence

So i am from an IT and my sis who is doing medicine asked for my help on this question i have no clue where to start actually. Please Help. So the question is: What is the nucleotide sequence for ...
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Why do some vaccines lose their efficacy with time?

Why do some vaccines lose with time their efficacy? The two obvious examples that I have in mind are influenza and tetanus. The former case is clear, as influenza virus undergoes frequent gene ...
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Questions regarding transformation in bacterial cells

First off, in transformation the donor DNA aligns itself with the complementary bases in the recipient DNA. Now a "perfect" alignment of the donor DNA ( Sorry if my terminologies are ...
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How to attract Cyanobacteria?

I would like to attract cyanobacteria in one spot on an object (e.g. cloth,) instead of having it swim in the media. my current method is to pour it on the object in a beaker and wait for some of them ...
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Why does touch the face make more likely to be infected?

Why is it risky to touch the face although there is no direct contact with into the nose, eyes and mouth? Is there any possibility that pathogens infect us from our facial skin? Suppose we have ...
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What is the rate of bacterial cell death due to viruses on land?

"The rate of viral infection in the oceans stands at 1 × 10^23 infections per second, and these infections remove 20–40% of all bacterial cells each day." - https://www.nature.com/articles/...
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What compounds are consumed/produced by Staph. aureus grown on Mueller Hinton agar over time?

I wonder what are the essential components on Mueller Hinton agar that S. aureus consumes and produces. I imagine there are preferred energy sources and as soon as those compounds run out the organism ...
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How to get Bacteria to attach to an Object?

I have cultured Cyanobacteria (in a media) in a beaker. I want that bacteria to attach to a cloth (e.g. Carbon). My current method is using a syringe to slowly push the bacteria to one side of a ...
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Can bacteria be killed by purely physical trauma?

In a question over on Skeptics Stack Exchange, I half-jokingly listed "blunt force trauma" as a means by which a bacterium could be killed. That makes me wonder if it really is only a joke ...
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How can life survive in the water under the Antarctic ice? [closed]

If there is life in the lakes under the ice of Antarctica then how can those bacteria survive? Where do they get energy from? https://www.google.com/amp/s/www.livescience.com/amp/64501-buried-lake-...
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Can a bacterium infect another bacterium?

I researched about it by searching on Google and reading some bacteriological articles, but I did not get any answer. I also asked some of my teachers, and they were also a bit confused. Some said ...
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"Bacteria don't have membrane-bound organelles." Are sulfur bacteria or Cyanobacteria exceptions?

Can bacteria have membrane-bound organelles? I read this many textbooks: Bacteria cells are simple cells that do not contain a nucleus or other membrane-bound organelles. However, they do contain ...
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Low-cost simple water testing for faecal contamination

I live in Indonesia and there are lots of sewage pipes discharging directly from homes/restaurants/etc directly into the river. It's also common to wash clothes and so on, so there is likely detergent ...
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Do most bacteria have plasmids?

Do most bacteria have plasmids? I googled that and I found this Yes, Plasmids naturally exist in all bacterial cells. Here But I know some of them don't! Is this sentence scientifically correct? ...
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Why does vacuum sealing of foods prevent spoilage from anaerobic bacteria?

I have noted that many purchased food items in containers only require refrigeration after they have been opened, thus eliminating the vacuum seal. Air contains bacteria and fungus spores that enter ...
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Are diplobacillus physically connected?

I know that a diplobacillus is a bacillus bacteria that has a buddy 'attached' to it. What I haven't been able to determine is exactly how is it 'attached'? Are their cell membranes fused - are they ...
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Horizontal gene transfer in a bacteria

I encountered an old examination question: Which of the following is not required in Bacteria for horizontal gene transfers? Infection 2. Translation 3. Transformation 4. Conjugation 5. Transduction ...

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