Questions tagged [bacteriology]

A subdivision of microbiology dedicated to the study of bacteria.

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How effective is rinsing in removing microbes from surfaces

Microbes including viruses stay alive on surfaces depending upon the type of the surface like steel, wood, plastic, glass, marble etc. How effective is rinsing these surfaces with tap water in ...
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How quicky does dental pH change after eating?

I would like to know how quickly after eating I can expect the pH do change in my mouth, and how quickly this will cause dental damage. Obviously any answer to this will have plenty of caveats (diet, ...
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How is minimum infectious dose defined?

I'm a bit confused by the concept of a minimum infectious dose (MID). It seems from what my research so far has turned up, that while any dose greater than 0 could potentially cause an infection, for ...
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Can I find out what resistance bacteria has based on strain? where do I look for that info?

I have a list of strains across various organisms for example: Escherichia coli - ATCC 25922, CGSC 6152, W3110RL. Pseudomonas aeruginosa - ATCC 27853, PAO1, APAE1111. I'm failing to find any ...
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characterising bacterial growth characteristics, recommendations?

To characterise a bacterias growth habits, we can obviously grow it in various medias and check the optical density at certain time intervals (with parallel). I understand this, and have no problems ...
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Optimal bacterial vegetative cell inactivation method(s) after subtilis spores germinated under pressure

I am writing a PhD research proposal and being a newbie in the field I am lost a little bit in the bacterial vegetative cell inactivation methods in foods. I should propose two main methods to my ...
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Is there a bacterium that became a virus?

Is it possible for a virus to be a descendant of a bacterium that was not through horizontal gene transfer? How I think this could happen: Suppose a species of bacteria lives in an environment where ...
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What are the methods to quantify the bacterial adhesion rate for the biofilms grown on coupons in CDC or annular bioreactor, non-invasively?

I'm interested to know that if I grow the biofilm on coupons in the CDC bioreactor at a particular shear stress, and after say 48 hours, I take out the coupon from the bioreactor to quantify the ...
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What is the protein composition of complete Freund's adjuvant?

The complete form of this adjuvant uses heat inactivated and dried mycobacteria. However, to rule out cross-reactivity with an antibody, I would like to know which proteins are left in the adjuvant. ...
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What is the principle behind Microagglutination test (MAT)?

Could some one please help me understand the principle behind the MAT. Also, is it only used to detect leptospira or can it be used to detect other pathogens as well? Kindly share links to papers if ...
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Is there a vaccine against the plague (Yersinia pestis)?

There seems to be recurrent events of infections of the plage (Yersinia pestis), from the well known Justinian plague to the Black Death and to recent years. In fact, two cases were reported in China ...
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How antibodies are produced in our body against intracellular proteins of infectious bacteria?

When an infectious agent invades our body, then surface antigens of the infectious agent are detected by our immune system and B-cells get activated. However, we do have antibodies in our blood ...
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Testing the affects of electro magnetic resonance on bacteria and viruses

I'm a student interested in testing the effects of electromagnetic resonance on bacteria and viruses (as a personal project). Specifically, if EMF induced resonance can be used to kill bacteria and ...
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Is there a critical size for a bacteria colony forming?

I am a maths student doing a project based on modelling bacteria and phages. In one of my models the bacteria population reaches a population size of ~450 cells. Would a colony of bacteria this size ...
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1answer
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Growing Mouth Bacteria in a bowl of sugar water

I want to teach my nephew why brushing in necessary and how sugar in mouth reduced by bacteria to acid. I plan to put sugar mixed water in bowl and take swab from his mouth and dip in bowl, and test ...
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Why doesn't infection with Clostridium Tetani immunize you?

What Immunity to COVID-19 Really Means - Scientific American It is less clear what those antibody tests mean for real life, however, because immunity functions on a continuum. With some pathogens, ...
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I need to identify the genus of this colony just by its morphology, can somebody help me?

it was grown on TSA it's a mesophilic bacteria. (it was contaminated by E. coli, so ignore the clearer and smoother colonies).
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How to prepare bacterin using formalin(formaldehyde)

I am finding how much formalin I have to use to make bacterins for vaccine experiments. I searched for it in google but I can't find about specific procedures like the concentration of formalin and ...
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Can far-UVC light be safely used as germicide? (help understanding a paper)

I've been trying to familiarize myself with the literature on far UVC light as a germicide. My question mostly pertains to figure 4 of this paper. The paper investigates the efficacy of 207 nm light ...
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Is Klebsiella pneumoniae found in the throat of healthy people? [closed]

Is Klebsiella pneumoniae found in the throat of healthy people?
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Can virus and bacteria move on the surface? [duplicate]

Do viruses/bacteria have some motive mechanism? I mean if the virus/bacteria reach some surface by touching, can it move on that surface? Life example: suppose the cooler is not so clean and has ...
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How to convert AU/ml of bacteriocin into zone of inhibition in mm?

My questions is about measuring antimicrobial activity against bacteria on petri plate. Is it possible to convert or somehow get zone of inhibition in millimetre from AU/ml?
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Can viruses stabilize and increase the shelf life of wine and other foods?

When you make wine, you use the yeast Saccharomyces cerevisiae to ferment sugary juices. After you're done, you want to avoid them doing anything by using some chemicals etc. There are also other ...
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Relationship between bacteria size and temperature?

I'm, doing a research in a TOC (total organic carbon) degradation in a BAC (biologically active carbon) filter for the grey-water treatment. Recently, I started to wonder if there is a relation ...
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A question about microbes growing media

I was told in my microbiology course that blood agar can be put in GasPak system (anearobic) to grow strict anaerobes, but why would someone use blood-dependent bacteria in this experiment as these ...
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Salt water swish for dental health [duplicate]

I was wondering as to why warm water is told for salt water gargle and not cold water, does warm water has more therapeutic effect? Also does salt really work, excess salty water might kill bacteria ...
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Why do gram-negative bacteria attack the digestive system more than gram-positive ones?

I was researching for a biology project on the subject of contagious infections of the digestive system (mainly the intestines) and almost all of the bacteria that came up (E.coli, Shigella, Cholera, ...
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small airborne microorganisms, are in many instances attached to bigger particulate substances?

From https://biology.stackexchange.com/a/90193/56476 very small airborne microorganisms, are in many instances attached to bigger particulate substances in the air, whether liquid micro droplets or ...
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From a hosts point of view, which comes first, effect of toxins on pathways or identification of toxins by immune system?

From a pure biology perspective, when a pathogen (gram-negative bacteria) affects a host by secreting toxins, what comes first, the effect of the toxin on the pathways or identification of the toxin ...
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Does parasitism, one of Bacteria's lifestyle?

The parasite is an organism that lives in or on a host. It depends on its host for survival. Bacteria lives in decaying organic matter, within human organism (colon, oral cavity). It can be a ...
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Is bacteria capable to learn?

Are bacteria chemicaly guided or there is some kind of complex electronic system that let them learn the envirement?Thanks.
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Will all bacteria become resistant against all antibiotics in the long term?

(I asked the same at medicalsciences beta, but I expect a quite different perspective on it here) This is about bacteria that can infect humans, and their multi-resistance. The evolution of bacteria ...
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Air purifier agains bacteria and viruses?

We would buy a mobile air purifier in our home. Against the smoke of the city, etc. It would have a HEPA + activated carbon filter. They are marketing it as: "filtration process captures 99.97%...
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How can I propagate a community of anaerobic bacteria sampled from mud in a bog?

I'm doing research on anaerobic bacteria. In the fall I took mud samples from a bog and have been working with them but I didn't take enough. Now winter has rolled around (Im in Canada) and all the ...
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Could Cyanobacteria farms help dilute pollutants in the atmosphere

If I understand correctly, roughly ~2.8 billion years ago cyanobacteria started pumping large amounts of oxygen into the atmosphere. Using modern industrial processes could this be emulated by ...
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Can bacteria pick up lethal plasmids?

I am sorry if this question is too general, and does not have any concrete answer. I was explaining to my non-biology-background friend about plasmids and how they are picked up by bacteria from the ...
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How do scientists kill the bacteria they themselves made resistant?

I was reading this article on researching bacteria resistance to silver by removing some of their genes. Researchers then used "colony-scoring" software to measure the differences in growth and ...
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How does a “chromogenic mixture” produce such pronounced color differences in this Agar Art?

The Forbes.com article Bacteria Become Art Tools In Annual Agar Art Competition says: ...People’s choice winner Zita Pöstényi, a microbiologist at SYNLAB Hungary Ltd, used three agar plates and ...
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Why is an antibiotic-resistance gene required during transformation of recombinant plasmid?

In my biology textbook, it said that in transformation, the recombinant plasmid containing an antibiotic-resistance gene act as a 'selective marker'. That means that plasmid in transformed bacteria ...
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A theory about the possible connection between protists and first animalia

I learnt that organisms within Kingdom Animalia can be either microanimals or (nonmicro)animals. a microanimal is any Kingdom Animalia organism that in general cannot be seen by a human eye without ...
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What are the sizes of individual cells of the cyanobacterium Nostoc spheroides?

In this article Nostoc spheroids are described as edible blue-green algae, but they are mostly named cyanobacteria, which are prokaryotes. In the above and other articles about Nostoc spheroids only ...
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What's the meaning of the label MRE and a number like 162 or 600 in the names of bacteria?

I've stumbled across labels like E. coli MRE 162 or E. coli MRE 600 for different kinds of E. coli bacteria. What's the meaning of MRE and the numbers like 162 or 600 in this context?
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What is the simplest way to demonstrate the germicidal property of UV light?

This is intended for a high school project. I am familiar with the one that studies growth of e coli over time in a petri dish but I a bit concerned about as to how safe a demonstration of this nature ...
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Ideas for microbiology lab. Antimicrobial properties of cutting boards

I am an undergraduate student in Biology and I am trying to design a microbiology lab that will takes 3 weeks to carry out (1-2 day each week). I am going to examine the antimicrobial properties of ...
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Are controls needed for urine cultures?

If testing for bacteria (gram-negative) in a urine culture, are negative or positive cultures needed? I could not find anything that says controls are needed unless results seem to be contaminated. ...
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Neisseria meningitidis transformation into a pathogen

I read the wikipedia article about Neisseria meningitidis, and it says that N. meningitidis is a part of the normal nonpathogenic flora in the nasopharynx of up to 5–15% of adults In some cases ...
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Scientifically testing when milk goes bad

How could I test if milk has gone bad scientifically? Performing a taste test on the milk yields a subjective interpretation as to whether the milk is good or not. Is there any objective metric that I ...
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Which bacteria takes the longest to double?

Currently I am writing a lab report on the enumeration of bacteria. E.coli takes about 30 mins to double. That is going from 1 bacteria to 2 bacteria. I want to know which bacteria takes the ...
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How can Haemophilus influenzae survive and grow alone in the human body?

Haemophilus influenzae gets its name from the fact, that it requires nutrients from blood in order to grow. More specifically, I see it mentioned that in vitro they often exhibit the pheomenon that ...
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Why is synthesis of tetanospasmin advantageous to C. tetani?

The tetanospasmin is a neurotoxin synthesised by some strains of C. tetani. It is the factor causing tetanus, but what is its role for the bacteria itself? I do not believe the main objective of C. ...

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