Questions tagged [bacteriology]

A subdivision of microbiology dedicated to the study of bacteria.

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6
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1answer
178 views

Why are O-antigens and H-antigens on (certain) bacteria called O and H?

Somehow, despite extensive searching, I cannot find an answer.... Does O stand for oxygen and H signify hydrogen?
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Why are bacteria/viruses not a common issue to plants? [closed]

I like growing a few plants in my living room. In some videos I watched about gardening, it's recommended to cut the plant for various reasons. Very often, it is recommended to sterilize the knife. ...
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Can antibiotic resistance cause problems for the microorganisms producing them? [duplicate]

My understanding is that antibiotics are naturally produced by microorganisms as a defensive measure against certain kinds of bacteria. For example, some Penicillium molds produce penicillin. Clearly, ...
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1answer
39 views

How to take professional SEM images of bacteria?

I am new to using SEM microscope I would like to take clear pictures of Bacillus subtilis. My sample is dropped on a piece of paper and carbon-coated. The picture is showing crumbs of something (maybe ...
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2answers
134 views

Why do bacteriophages look like tiny little robots? [closed]

I get it, evolution is funny and works in mysterious ways. But come on. You can't tell me this thing doesn't look like a nanobot. Why do bacteriophages look this way? With their cylindrical heads and ...
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If brine salt percentage is between 1 and 2 percent, can Clostridium botulinum growth prevail over Lactobacillus acidophilus?

After searching in various websites, I found mostly that Clostridium botulinum doesn't grow easily in a salty environment, and that it needs a pH from 4.6 to up; as I am lactic fermenting some bell ...
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31 views

Doubts about what is growing in my bacteriological cultivation

Foreword: I don't belong to the field at all, I am a high school student with a passion for science so I apologise in advance if I formulate the question incorrectly or make some mistakes. Out of ...
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2answers
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Do bacteria produce proteins other than ribosomal proteins when grown in wrong culture media?

Someone I know has done an experiment using pure culture and given it to me for rna-seq analysis. But there are a few problems the bacteria she gave me as reference was initially a Staphylococcus sp. ...
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How does Propionibacterium acnes survive the antibacterial effects of sebum?

Sebum has antibacterial properties due to sapienic acid and oleic acid according to this article on sebum. According to the same article, desaturation of fatty acids increases acne development. ...
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0answers
65 views

Do bacteria select for "soap resistance"?

When people wash their hands with soap, this limits the spread of bacterial infections. That much, I am pretty convinced of. Duh. What I don't get is how this doesn't lead to evolutionary pressure on ...
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55 views

How typhoid fever severe case's intestine perforation occur? (non trauma)

In typhoid severe case, intestinal perforation occurs. As stated here [4, 6]. Intestinal perforation is a serious complication of typhoid fever My question here is, how does the bacteria (Salmonella ...
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1answer
96 views

How to measure the pH of a bacterial species?

I would like to calculate the pH of a certain bacteria species before after an experiment. I was reading about the pH cell of bacteria and I found out about Bacterial Intracellular pH which I ...
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1answer
90 views

Mutations in a Petri dish overnight

How long does it take for a bacterial culture in a Petri dish to experience all possible single base pair mutations? Can 12 hours be enough? I want to get an intuition for whether a given mutation is ...
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1answer
70 views

What is sigma factor PvdS?

I am using MEME suite tool for de novo motif discovery and when I use TomTom to compare my motifs with known motif database, I find that my genes could potentially be upregulated or downregulated by ...
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1answer
90 views

Is there any alternative to peptone and beef or yeast extract?

I am trying to start a small microbiology project and I need a growth culture for bacteria. I do not want to use the costly nutrient agars you can buy online. I would like to create my own nutrient ...
12
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1answer
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Will proper autoclave treatment really "inactivate all resistant bacterial spores"? If not, how widespread are autoclave-resistant bacterial spores?

Wikipedia's says: A widely used method for heat sterilization is the autoclave, sometimes called a converter or steam sterilizer. Autoclaves use steam heated to 121–134 °C (250–273 °F) under ...
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0answers
31 views

What is the structure of PduK in the Pdu BMC?

My lab is creating 1,2-propanediol utilization bacterial microcompartments (Pdu BMCs) with an operon containing genes for PduA, PduB, PduJ, PduK, PduN, PduU, and PduT. According to Mayer et al., all ...
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157 views

Is it known how some heat-resistance Bacillus spores repair their DNA after having been heated to 420 °C? (but not much higher)

Background Discussions below several recent posts in Space SE (links below) indicate that bacterial spores are a serious problem when considering how to prevent a future spacecraft mission to ...
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1answer
29 views

Disposing biohazardous material

I had conducted some disc diffusion tests with E.coli and S.epidermidis. Now, I wanna dispose these petri dishes and was wondering the correct way to do it. Many websites mention adding bleach to the ...
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21 views

Why dehydration is linked with heat resistance in bacteria and fungi?

According to wikepedia Dipicolinic acid results in heat resistance of endospore by doing dehdration of the endospore Dipicolinic acid forms a complex with calcium ions within the endospore core. This ...
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1answer
50 views

How to create long term stock for Bacillus subtilis?

I am trying to create a long-term stock for Bacillus subtilis (Bacterial glycerol stocks). I have looked online for guides and step-by-step tutorials and I found that they don't say where the sample ...
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1answer
30 views

When Bacillus subtilis form spores vs die?

In my understanding: sporulation in Bacillus subtilis involves the differentiation into two cell types, the endospore, and the mother cell. The endospore coat is a multilayered shell that protects the ...
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0answers
26 views

Cyanobacteria and the ratio of Iron to Phosphorous

In listening to a recorded lecture that touched on the Great Oxidation Event, a casual remark was made to the effect that the shifting of the Earth's constituents to more availability of phosphorous ...
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Methods for getting clean C. difficile spore preps without spores clumping together

I'm a microbiologist recently who recently started working on an ongoing C. difficile project for the first time. One of the things I need to do is verify the lower detection limit of my qPCR ...
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Does Tryptic Soy Agar (TSA) contain L-cysteine? (Question about Legionella)

I ask because Legionella sp. requires the presence of cysteine. If TSA does contain L-cysteine, is it sufficient to grow Legionella?
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1answer
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Is there a specific English term for this vessel for microbial sampling?

In Russian, such a container (see pictures) for microbial sampling is called бакпечатка (bakpechatka), as I learned just now. I've no idea about the etimology of the Russian term. Probably "bak&...
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Do E. coli change color (from white/grey to yellow) when they grow older?

In my experiment, I had white colonies initially and they turned yellow. So I thought it´s natural that mature E. coli colonies are yellow and young are white. But it could be that I had two types of ...
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1answer
86 views

What determines the physical appearance of a tangible amount of bacteria or viruses?

As in the title, I was wondering how would feel an amount of one type of bacteria or virus, big enough to have a tangible size of the sample. What color, state (liquid, solid), textures, smell ecc. of ...
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What are the causes of gene amplification in archaea?

I was studying this article about Genome Sequencing of a Genetically Tractable Pyrococcus furiosus Strain Reveals a Highly Dynamic Genome in order to try to extrapolate some features that could ...
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Co-culture models of bacterial invasion: where to find them?

I am interested in models showing how pathogenic bacteria (namely Escherichia coli O157) can overcome commensal species (thus, causing a disease). This is a basic concept in biology but I can't find a ...
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1answer
829 views

Novel bacterial strains of bacteria first isolated on the International Space Station, did the space environment lead to these genetic changes?

Question Methylobacterium ajmalii sp. nov., Isolated From the International Space Station (Bijlani et al. Frontiers in Microbiology, 12, p. 534, 2021) is a thorough analysis of "novel strains&...
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2answers
168 views

In what stage of bacterial growth can penicillin inhibit the growth of the cellular wall?

The bacterial growth has 3 main stages: lag-phase, log-phase and stationary phase. I was wondering in which one of them penicillin can inhibit the growth of the cellular wall of bacteria and why. I ...
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31 views

What is this species of bacteria?

Can I ask what is the species of this bacteria? A comment there says that it is probably E. coli. Disclaimer: I don't have an idea about the given video of bacteria as we are asked to solve for the ...
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Bacterial endosymbiosis of bacteria

Endosymbiosis was crucial to the development of complex life and it continues to happen to this day. The origin of mitochondria see bacterial guests in archaea hosts, some of which later took in ...
4
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1answer
170 views

Alternative dyes for Gram staining

In the Gram stain, is there any replacement for primary stain, secondary stain, decolourizer and mordant? What results will the replacements produce? I found that crystal violet can be replaced with ...
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1answer
49 views

Calcium ions and bacterial transformation

What is the mechanism by which calcium ions and heat treatment allow the bacterial membrane to become permeable, allowing the uptake of plasmids?
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1answer
2k views

Are there phage-eating bacteria?

If phages (bacterium-eating viruses) prowl on bacteria, are there bacteria (or other micro-organisms) that hunt phages for food? They are rich in proteins anyway... Are there studies on this subject? ...
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1answer
104 views

What fraction of bacterial proteins are membrane bound?

Almen et al. showed that around 27% of the human coding proteins are membrane bound. How does this compare with bacteria such as E.coli or B.subtilis (or any other bacteria)? Is there a paper out ...
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1answer
55 views

Skeletisation in space

A space pod containing an astronaut drifts out to space and cannot be rescued. The space pod contains no insects, and the only bacteria present are those originally brought in by the astronaut. There ...
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1answer
52 views

Will wiping a phone down with isopropyl alcohol daily breed bacteria resistant to disinfectants?

The title says it all. If you disinfect something more regularly, aren't you just selecting for bacteria which are most robust to that method of disinfection? I've heard it said about some medications ...
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1answer
38 views

Does probiotics bacteria from yogurt live in the mouth too? Does it populate the mouth/throat or just the gut?

Yes I was basically just wondered this as I've taken probiotics in a pill form but those only go to the gut. I guessed the mouth needs probiotics too and I wondered if yogurt can accomplish that. I ...
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0answers
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How does lactate produced in the intestines get transported to the rest of the body?

Some gut bacteria known as lactic acid bacteria(LAB) are known to produce lactate in the intestines and they confer beneficial effects such as enhancement of the immune system, but I assume not all of ...
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1answer
39 views

Nucleotide Sequence

So i am from an IT and my sis who is doing medicine asked for my help on this question i have no clue where to start actually. Please Help. So the question is: What is the nucleotide sequence for ...
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Why do some vaccines lose their efficacy with time?

Why do some vaccines lose with time their efficacy? The two obvious examples that I have in mind are influenza and tetanus. The former case is clear, as influenza virus undergoes frequent gene ...
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49 views

Questions regarding transformation in bacterial cells

First off, in transformation the donor DNA aligns itself with the complementary bases in the recipient DNA. Now a "perfect" alignment of the donor DNA ( Sorry if my terminologies are ...
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1answer
49 views

How to attract Cyanobacteria?

I would like to attract cyanobacteria in one spot on an object (e.g. cloth,) instead of having it swim in the media. my current method is to pour it on the object in a beaker and wait for some of them ...
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26 views

Why does touch the face make more likely to be infected?

Why is it risky to touch the face although there is no direct contact with into the nose, eyes and mouth? Is there any possibility that pathogens infect us from our facial skin? Suppose we have ...
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What is the rate of bacterial cell death due to viruses on land?

"The rate of viral infection in the oceans stands at 1 × 10^23 infections per second, and these infections remove 20–40% of all bacterial cells each day." - https://www.nature.com/articles/...
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1answer
51 views

How to get Bacteria to attach to an Object?

I have cultured Cyanobacteria (in a media) in a beaker. I want that bacteria to attach to a cloth (e.g. Carbon). My current method is using a syringe to slowly push the bacteria to one side of a ...
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5answers
9k views

Can bacteria be killed by purely physical trauma?

In a question over on Skeptics Stack Exchange, I half-jokingly listed "blunt force trauma" as a means by which a bacterium could be killed. That makes me wonder if it really is only a joke ...

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