Questions tagged [bacteriology]

A subdivision of microbiology dedicated to the study of bacteria.

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How do prokaryotes respire? [on hold]

Can someone PLEASE answer...how do prokaryotes respire? I have checked all other answers but can not find a simple and easy way of explaining how prokaryotes respire.
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Sensitivity of fever as an indicator of infectious disease

What is the sensitivity or likelihood ratio of fever as an indicator of an infectious disease? Where can I get the number from?
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Salt Bridge for Microbial Fuel Cell: Full or Partly Full?

I'm making a microbial fuel cell out of benthic mud and salt water in containers. My salt bridge will be made of agar and table salt solidified in PVC pipes. Does the salt bridge connecting the anode (...
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What are the survival conditions for Clostridium botulinum?

I notice that the incidence of poisoning from the activity of Clostridium botulinum is quite rare. For example, in 2015 there were only 7 incidents of foodborne botulism poisoning identified even ...
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2answers
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Are there any non-pathogenic gram-positive bacteria in human gut?

I wish to know if there are any non-pathogenic (not even opportunistic pathogen) gram-positive bacteria in the human gut? Is there any reference that shows there exists a non-pathogenic gram-positive ...
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1answer
21 views

Immune response to IgA positive bacteria

If certain bacteria can be coated with IgA in vitro, does that mean they are likely to elicit an IgA immune response? Edit I'm working on a project that involves IgA-Seq analysis. Bacteria are coated ...
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1answer
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Can you design a bacteriophage that attacks the part of the bacteria that makes them antibiotics resistant?

I read in a newspaper about a case where they had found a virus that could attack a specific form of antibiotics-resistant bacteria and managed to save a girl from a certain death by "infecting her" ...
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what are best methods for mrsa screening in hospital?

methods for screening MRSA are based on PCR (mecA detection) and MRSA latex test and culture. I need to know sensitive, specific and cost-effective methods for diagnosis MRSA infection on one work day ...
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52 views

Could bacteria and phages be found in tissues?

in the literature, there are several studies of the gut virome and microbiome, for instance Reyes et al. on Nature (https://www.nature.com/articles/nature09199?error=cookies_not_supported&code=...
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Type of Immunity- Bacterial septicaemia

what type of immunity (Non-specific, Anti-body mediated, cell-mediated) is expressed in Bacterial Septicaemia (Bacterial contamination in systemic circulation)?
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Size distribution of all known species in the domain bacteria

I have found the smallest bacteria are around 200nm (e.g Mycoplasma genitalium an ultramicrobacterium) and largest are around 700um (Thiomargarita namibiensis). I am looking for a graph that ...
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How do I homogenise a small pellet of 10 to 50 cells within less than 50 µL?

I have a problem with declumping/ homogenising bacterial cells in small volumes (10 to 50 µL). I know that I can get all of my cells out of the tube, but they are still stuck together and won't form ...
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How to grow Bacillus subtilis spores for making natto?

I enjoy making fermented foods, one of them is natto, which is cooked soybeans fermented with B. subtilis. I usually buy the B.s. spores online, but they are somewhat expensive and often hard to find....
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What is a secretion target family protein?

I came across this link: https://www.genome.jp/dbget-bin/www_bget?btw:BF38_3398 What is this protein, is it a secreted effector protein or is it something else? What is the difference between type ...
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What is the frequency of AUG near-cognates (as start codon) in ‘Leaderless’ mRNA?

In typical bacteria that use SD-dependent translation initiation, the AUG start codon may sometimes be replaced by near-cognates (GUG, UUG, etc.). The frequency of these near-cognates is somewhat ...
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If a bacterium had a protein on its surface that humans also have, would it cause an autoimmune disease?

Suppose that a bacterium happened to have a protein on its surface. This protein can also be found in the human body. If this bacterium were to then infect a human with an otherwise normal immune ...
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What is the role of lactic acid bacteria (such as L. acidophilus and L. bulgaricus) in glycolysis during yogurt fermentation?

I am trying to prove that an increase in monosaccharides (glucose, fructose, galactose) that have been added to milk during fermentation will result in a decreased pH. My current argument is: "The ...
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1answer
55 views

How did sneezing evolve?

Did we develop the sneezing mechanism to get rid of pathogens like bacteria and viruses in our nose? Or did pathogens develop a way to make us sneeze to propagate themselves? Or did we develop the ...
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1answer
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What happens to Bacteria at a low pH?

I am conducting an experiment on how different pH levels effect the growth of E.coli. After researching, it is predicted that the most acidic pH level (pH 2) will be most effective at killing (or at ...
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Is there a glossary about which infrared (IR) colors which body parts reflect?

I am doing medical research and I'm trying to find parts of the body that look about the same to the human eye, but reflect different "colors" in IR, so that using a light source and a camera of that ...
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How does the symbiotic bacteria reaches a human caecum

My thought on this matter is that we have got a very high pH of about 1.8 in our stomach.So,most of bacteria who came in through food or just open mouth should be killed by it.So,how does a bacterial ...
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38 views

What happens to Bacterial Cells stored at 4 degrees?

4C is when water is most dense and is not so low as to cause ice crystal formation. For short term storage bacterial cultures are often simply kept in a fridge at 4C. I guess that this significantly ...
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Thermophilic Total plate count with PCA with Triphenyl tetrazoliumchloride as supplement

Hello I'm am a fourth year microbiology analyst and I'm busy with finishing my final internship. During my final internship I have to complete a own assignment. the assignment I got was to find out if ...
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87 views

Loss of antibiotic resistance due to long term storage

How possible is it for antibiotic resistant bacteria stored for a long time to lose such resistance?
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25 views

How reliable is the life cycle information on PhagesDB?

If I'm understanding this correctly, PhagesDB users choose the Cluster/Subcluster during the upload, and the life cycle type is assigned solely based on this data. How reliable is that information? ...
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1answer
128 views

Killing microorganisms on agar plates?

If I grow microorganisms on agar plates and I expose them to UV light. How exactly will I know that the microorganisms have been killed? I was watching a video that showed spots in the petri dish ...
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Could bacteria theoretically evolve to survive in space? What is the maximum altitude in the atmosphere that anaerobic bacteria can survive?

There is no edge to the atmosphere, and therefor I expect there would be a gradual reduction of concentrations of bacteria as we ascend up into space. Logically anaerobic bacteria should be able to ...
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1answer
47 views

Home bacteria culture at home?

Can simple bacterial culture be done at home using food agar as the medium (with some additives found in the store)?
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27 views

E. Coli growth inhibited in Powdered Honey

I ran into problem with my method validation since the E. Coli that I've spiked into my Honey Powder sample can't grow in the media. The media I've used is Merck CCA (Chromogenic Coliform Agar). I've ...
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1answer
52 views

Are the bacteria doing the same photosynthesis as plants do?

I mean Plants during photosynthesis produce oxygen but Do bacteria also produce oxygen during photosynthesis or produce other gases? If yes , how do these bacteria process photosynthesis by ...
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1answer
49 views

Why is there such in interest in antibiotics against Pseudomonas?

I keep finding information on anti-pseudomonal antibiotics, or information specifying the antibiotic has anti-pseudomonal activity. Is there anything peculiar to this bacterium when compared with ...
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1answer
43 views

How can i count my E. coli colonies?

I did a test which involved growing e. coli on a petri dish. I did not dilute the sample, and this resulted in growing hundreds of small, overlapping colonies. However, they still can be counted, but ...
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Why do we have an immune system?

My daughter asked a question that I could not answer. If you have a cold, you have symptoms such as fever, cold and sore throat. These symptoms, however, are the reactions of the immune system to ...
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What happens when the bacteria in the petri dish lack nutrients for long time?

If I put some E.coli in a petri dish filled with limited nutrition in room temperature and forget them for months or years. What will happen in the final after they overgrowth and used all nutrition ...
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What is the difference between transport proteins and effector proteins in gram-negative bacteria?

When considering pathogenic gram-negative bacteria, is there any difference between the function of transport proteins and effector proteins? Or are they of the same functionality? Any reference ...
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Can a plastic degrading bacteria project be recreated in a school lab environment?

I am looking for a science fair project and am wondering if this could be a good idea. I am in the 10th grade. I am not exactly sure of the materials needed since this is just the beginning stages of ...
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Can a plastic degrading bacteria science fair project be recreated in a school lab?

i am just looking for a science fair project and am fascinated by this topic. i was wondering if a project concerning the isolation of plastic degrading bacteria could be done in a school lab. I am in ...
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3answers
292 views

can a bacterium cell become a cancer?

I don't mean if a bacterium can be the cause of cancer inside a human. But can actually a bacterium changes in the way as normal cells change into tumor cells? So gaining such characteristics of a ...
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1answer
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Psychrotrophic Bacteria

I want to know what gene or protein makes bacteria psychrotrophic, as opposed to regular bacteria that prefers warmer conditions. If you don’t know exactly, and you have suggestions as to where I can ...
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3answers
175 views

A Hadeeth on the fly

I think most biologists here on this site will not consider my question below to be unfit for posting on Biology SE. I came across this article about the hadeeth (a hadeeth is a saying of the ...
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What is the difference between enrichment media and selective media?

Enrichment media These media are used to suppress commensal bacteria while allowing the pathogen to remain viable and grow. [Source: Ananthnarayan and Paniker's textbook of Microbiology.] ...
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Searching a fructose degrading bacteria [closed]

my question is do you know a fructose degrading bacteria? Wich is straitforward to use and cultivate? Wich shouldn't create excrements, that can't be removed simple.
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1answer
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How does anaerobic bacteria produce ATP for it's own cellular activities?

Anaerobic bacteria utilise glycolysis: Glucose + 2 P + 2 NAD+ => 2 ATP + 2 H + 2 NADH + 2 H2O + 2 Pyruvate followed by fermentation: Pyruvate + NADH => Lactate + NAD+ The resultant NAD+ formed ...
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What causes bacteria to produce a mixture of L-lactic acid and D-lactic acid?

Lactic acid is an organic compound with the formula CH3CH(OH)COOH. Lactic acid is chiral, consisting of two optical isomers. One is known as L-(+)-lactic acid or (S)-lactic acid and the other, its ...
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How do the bacteria producing bacteriocins protect themselves from it?

Whenever there is Col plasmid in a bacteria it will produce bacteriocins which will kill other bacteria around it. Why will the bacteriocin not kill the bacteria which has produced it? How does the ...
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1answer
98 views

Why reaginic antibodies are absent in these types of syphilis?

According to Textbook of Microbiology and Immunology 2e, Subhash Chandra Parija, pg.no; 375 These(reaginic) antibodies do not appear in early primary syphilis, latent acquired syphilis of long ...
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How to grow bacteria?

We have this science experiment on how to grow a bacteria, our teacher told us to use gelatin, beef stock, and sugar as an alternative for agar. I am confused on why we need gelatin, beef stock, and ...
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Vibrio vulnificus from liquid soap

At some point between 2002 and 2006 I attended a departmental seminar in the UK on Vibrio infections, and particularly on the (then relatively unheard of) V. vulnificus, which causes necrotizing wound ...
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Why aren't all infections immune-system resistant?

It's been less than a century since the widespread use of antibotics started, and already we're seeing bacteria that have evolved immunities to the antibotics we use. On the other hand, we've been ...
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Is it possible to prove that a hand sanitizer can kill 100% of a person's germs?

We have a project for Economics, and we need to advertise something that consumers will be willing to buy. My group thought of "improved" hand sanitizer. So, instead of it killing 99.9% germs, we ...