Questions tagged [bacteriology]

A subdivision of microbiology dedicated to the study of bacteria.

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What's the meaning of the label MRE and a number like 162 or 600 in the names of bacteria?

I've stumbled across labels like E. coli MRE 162 or E. coli MRE 600 for different kinds of E. coli bacteria. What's the meaning of MRE and the numbers like 162 or 600 in this context?
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What is the simplest way to demonstrate the germicidal property of UV light?

This is intended for a high school project. I am familiar with the one that studies growth of e coli over time in a petri dish but I a bit concerned about as to how safe a demonstration of this nature ...
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How long does it take the bacterium nasuia deltocephalinicola to self-replicate? [closed]

At which speed does the Nasuia deltocephalinicola replicate itself given optimal conditions?
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How is a bacterial strain defined?

When a species of bacteria is referred to by its strain, are they a clone of single founder or is a certain amount variation allowed?
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Bacterial Growth Formula

What are the units of $N$: the colonies of bacteria or the viable cell count? This is in regards to the bacterial growth formula used during serial dilutions, $$ N =N(0) e^{kt} \quad . $$
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Pink growth (aka pink slime) in bathtub or shower

I was wondering if anybody had studied or was knowledgeable about the pink growth that people often find around water fixtures in their homes, especially bath tubs and showers? My understanding is ...
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Ideas for microbiology lab. Antimicrobial properties of cutting boards

I am an undergraduate student in Biology and I am trying to design a microbiology lab that will takes 3 weeks to carry out (1-2 day each week). I am going to examine the antimicrobial properties of ...
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Blue light in a fridge. Is it really beneficial or just marketing?

Recently while visiting a shop with electronics I saw a fridge with a huge advertisment sticker going more or less like this: There's blue light source in this fridge so there will be less bacteria ...
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Are controls needed for urine cultures?

If testing for bacteria (gram-negative) in a urine culture, are negative or positive cultures needed? I could not find anything that says controls are needed unless results seem to be contaminated. ...
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How do anaerobic bacteria produce ATP for their own cellular activities?

Anaerobic bacteria utilise glycolysis: Glucose + 2 P + 2 NAD+ => 2 ATP + 2 H + 2 NADH + 2 H2O + 2 Pyruvate followed by fermentation: Pyruvate + NADH => Lactate + NAD+ The resultant NAD+ formed ...
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Neisseria meningitidis transformation into a pathogen

I read the wikipedia article about Neisseria meningitidis, and it says that N. meningitidis is a part of the normal nonpathogenic flora in the nasopharynx of up to 5–15% of adults In some cases ...
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Scientifically testing when milk goes bad

How could I test if milk has gone bad scientifically? Performing a taste test on the milk yields a subjective interpretation as to whether the milk is good or not. Is there any objective metric that I ...
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Which bacteria takes the longest to double?

Currently I am writing a lab report on the enumeration of bacteria. E.coli takes about 30 mins to double. That is going from 1 bacteria to 2 bacteria. I want to know which bacteria takes the ...
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Salt Bridge for Microbial Fuel Cell: Full or Partly Full?

I'm making a microbial fuel cell out of benthic mud and salt water in containers. My salt bridge will be made of agar and table salt solidified in PVC pipes. Does the salt bridge connecting the anode (...
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Is iodised salt harmful to the lactofermentation process?

I was discussing sauerkraut recipes with someone on IRC a minute ago, and they warned me not to use iodised salt in the process. My first (and so far, the only successful) batch is one I made with ...
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34 views

Immune response to IgA positive bacteria

If certain bacteria can be coated with IgA in vitro, does that mean they are likely to elicit an IgA immune response? Edit I'm working on a project that involves IgA-Seq analysis. Bacteria are coated ...
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How can Haemophilus influenzae survive and grow alone in the human body?

Haemophilus influenzae gets its name from the fact, that it requires nutrients from blood in order to grow. More specifically, I see it mentioned that in vitro they often exhibit the pheomenon that ...
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Can you design a bacteriophage that attacks the part of the bacteria that makes them antibiotics resistant?

I read in a newspaper about a case where they had found a virus that could attack a specific form of antibiotics-resistant bacteria and managed to save a girl from a certain death by "infecting her" ...
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What is the advantage of circular DNA in bacteria?

From what I understand, bacteria have circular DNA. What advantages does it have over linear strands like for eukaryotes? Do there exist bacteria with more than one ring of DNA?
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Why is synthesis of tetanospasmin advantageous to C. tetani?

The tetanospasmin is a neurotoxin synthesised by some strains of C. tetani. It is the factor causing tetanus, but what is its role for the bacteria itself? I do not believe the main objective of C. ...
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Could bacteria and phages be found in tissues?

in the literature, there are several studies of the gut virome and microbiome, for instance Reyes et al. on Nature (https://www.nature.com/articles/nature09199?error=cookies_not_supported&code=...
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How big can a colony/society get?

I hope this is the appropiate place for this question, asked from a math/physics perspective with little to no training in biology. Bacteria are very small and live in huge colonies, ants are bigger (...
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Home bacteria culture at home?

Can simple bacterial culture be done at home using food agar as the medium (with some additives found in the store)?
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I found this in my refrigerator water filter. Is it a biofilm? Is it a sign of dangerous bacteria?

When I dispense water form my refrigerator after changing my filter, the following slimy tube like deposits appeared. Does anybody know what this is? Could it be a biofilm growing in the water hose, ...
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What is a catalytic domain?

I have to answer a few questions from an article I'm reading, and I am just really confused on defining "catalytic domain." I have to relate it to the antibiotic Teixobactin. Is catalytic domain the ...
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Are PVC&PET bacteria found inside homes?

I am interested in weather PVC and PET eating bacteria can be found inside people's homes. The PET consuming ones were found in or near plastic waste dumps or in the ocean, but would they also have ...
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official authoritative source for bacterial shape classification

I'm a bit disappointed but after several hours of search, I could not find an official authoritative source for bacterial shape classification. I find a lot, I mean a lot of links but no original ...
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Are most pathogenic bacteria facultative anaerobes?

I know that S. aureus and most gram-negative rods are facultative anaerobes. In terms of number of species, are most human pathogen-associated bacteria facultative anaerobes?
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How many types of bacteria?

Bacteria are classifed on the base of food. Two types of bacteria, autotrophic and heterotrophic bacteria. Autotrophic bacteria that prepare own food or energy. Heterotrophic which cannot prepare own ...
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How do parasites “reprogram” brains?

Dicrocoelium dendriticum is a parasite that uses ants as a second intermediate host (which means that ants aren't its primary host/target, but are rather a temporary host to help reach their main/...
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326 views

Heat shock vs electroporation

I've been transforming E. coli via heat shock in order to insert oligonucleotides (around 50 nt); however, none of my experiments have given positive results so far. I begin to question the efficiency ...
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325 views

Serological assays measuring antibody response

Given that an appropriate immune response to a bacteria may be thwarted in an individual, including not producing all of the antibodies which are known to occur in people who have been infected, or ...
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What disease does Saccharopolyspora erythraea cause?

For an examination assignment I have to find a disease caused by the bacterial species Saccharopolyspora erythraea, but I have searched the internet and have found no report of patients being infected ...
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E. coli & aspartate

I am familiar with the linkage between aspartate/ligand binding, receptor methylation, and flagellar behavior re. style of locamotion, but I do not know what the bacterium does with the aspartate it ...
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Does drinking alcohol having UTI may help to kill the bacteria?

It's well known that ethyl alcohol kills bacteria. And amount of alcohol in urine may be detected by EtG tests (as long as 3-4 days after intake). Does drinking alcohol in reasonable amount by ...
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Is bacillus strain 2-9-3 really 250 million year old?

I've read that in the last decade (I believe) some scientists discovered a microorganism, which they dubbed bacillus strain 2-9-3, in a crystal of salt that they think is 250 million years old and ...
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Do bacteria exist which live exclusively on non-organic matter?

Are there bacteria which consume exclusively non-organic matter? Meaning that they do not require organic matter from other organisms to stay alive and reproduce? A completely pacifistic bacterium. ...
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Are there completely self-sufficient autotrophs?

Are there completely self-sufficient autotrophs which can survive and reproduce indefinitely by consuming exclusively non-organic matter (plus energy from sunlight or thermal vents etc.)? I'm asking ...
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Why don't we build up an immunity to sore throat?

We often get sore throats once or twice a year, and it clears in a few days sometimes without any antibiotics. I was wondering why doesn't our body become immune after clearing a sore throat?
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How long can Legionella survive at 60°C?

I'm coding some stuff for water heaters and one of the functions is anti-Legionella. Anyway, I've read on wikipedia that 90% Legionella dies in first two minutes if the water temperature is 60°C, but ...
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Why is it possible to make yoghourt with lactose free milk?

The function of the Lactobacillus bulgaricus and Streptococcus thermophilus cultures is to ferment lactose to produce lactic acid. The increase in lactic acid decreases pH and causes the milk to clot,...
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What enzyme or bacteria could dissolve plastic?

What enzyme or bacteria could dissolve plastic aside from acetone? Preferably, low-cost and environmentally friendly. Any suggestions would be helpful. I'm targeting for thin plastic (low-density ...
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Fermenting bacterial growth with potato dextrose broth

I am trying to propagate some strains of bacteria (L.Plantarum, B.Subtilis, L.Rhamnosus, B.Longhum, B.Bifidum) in potato dextrose suspension for my own consumption. I would like some guidance, ...
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How much of the weight of yoghurt is due to bacteria?

I recently started making all kinds of bacteria-processed foods by myself (such as yoghurt, but also Sauerteig and so on), and I found myself wondering how much of the weight of these products is ...
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Does a microwave oven disinfect food?

Imagine I am preparing food -- just about to put it into a microwave oven -- and some of it falls on the floor. Assuming it got some bacteria or other organisms (viruses?) on it, will the microwave ...
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What does the gene name “lexA” stand for?

It is an important gene expressed in E. coli that represses the SOS response and also the expression of lambda lytic phase genes. UV light and damage to DNA is responsible for its breakdown and hence ...
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What are the survival conditions for Clostridium botulinum?

I notice that the incidence of poisoning from the activity of Clostridium botulinum is quite rare. For example, in 2015 there were only 7 incidents of foodborne botulism poisoning identified even ...
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Are there any non-pathogenic gram-positive bacteria in human gut?

I wish to know if there are any non-pathogenic (not even opportunistic pathogen) gram-positive bacteria in the human gut? Is there any reference that shows there exists a non-pathogenic gram-positive ...
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Killing microorganisms on agar plates?

If I grow microorganisms on agar plates and I expose them to UV light. How exactly will I know that the microorganisms have been killed? I was watching a video that showed spots in the petri dish ...
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Any studies on the effects and/or benefits of oil pulling? [closed]

A friend recently recommended me a process called 'oil pulling' for my teeth as I am struggling with my dental health at the moment. The process involves using a tablespoon of some kind of vegetable ...