Questions tagged [bacteriology]
A subdivision of microbiology dedicated to the study of bacteria.
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Carcinogens or cancer causing agents also tend to be harmful to bacteria
I notice an interesting observation that Carcinogens or cancer causing chemical agents also tend to be very efficient bacteria killers. How true is this? And is there a biological explanation for this....
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How can metagenomic analysis be used to determine the native habitat of a bacterial species?
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A native habitat refers to the natural environment in which a particular species or organism has evolved and to which it is best adapted.
In the context of bacteria or other microorganisms, ...
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How are sewage, sludge and wastewater natural environments?
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Acinetobacter species can be isolated from such natural environments
as surface water, wastewater and sewage, healthy human skin, plant,
animal and food material as well as domestic appliances.
[...
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I accidentally plated DH5a E. coli and left them in the 4℃ for 12 hours, if I put them into the 37℃ incubator will they start growing?
I started ligation of my constructs late last night and was super tired, since I used all of my ligation mixture I would rather not have to re-ligate.
I did my transformation procedure as follows:
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Is the presence of a specific gene in one bacterial species enough to conclude it has the same function in another species within the same genus?
I was wondering if the answer should be more or less conclusive given the following givens and some of their implications that I feel by gut feeling could play a role in the answer:
The species ...
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Is bacterial "colonization" restricted to human body surfaces not the deep tissues?
This seems to be the point where the perspective of human beings from a medical viewpoint intersects with that from an ecological perspective.
Colonization is the presence of bacteria on a body ...
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Post-Translational Modification in insulin production
When searching "How is insulin produced commercially" on Google, most results simply say something along the lines of "The insulin gene is inserted into a bacteria, which then express ...
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What is (are) the species responsible for Lake Natron red waters?
Lake Natron is an alkaline lake located in northern Tanzania. It is known for its red waters, as seen on this image:
Natural-color Landsat 8 image showing Lake Natron in March 2017. NASA, public ...
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Metabolism of Purple non-Sulfur bacteria
I am a high school student who is currently experimenting with hydrogen sulfide absorption using bacteria. I know species of purple sulfur bacteria or green sulfur bacteria are able to oxidize ...
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Can saltwater bacteria grow in petri-dish using the same agar nutrient media?
I am planning on growing bacteria from a seawater sample to do a research project on the relationship between pH (lowered using sulfuric acid) and the bacterial concentration of the water sample (...
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How do I grow 5 liters of purple non-sulfur bacteria?
I am running experiments for a project that requires large amounts of purple non-sulfur, Cyanobacteria and nitrogen fixing bacteria. I plan on growing all the species in separate tanks. The species' I ...
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What is a reference strain in DNA-DNA hybridization DNA groups?
Results
DNA groups identified:
All intra-group relatedness values are shown in
Table I.
By means of reference strains, most of the DNA groups could be identified as groups described by Bouvet & ...
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Where does Actino- come from in the genus Actinomycetota?
This comment under Bacteria trapped in crystal inclusions found 'alive' after 50,000 years - what were they eating all that time? links to Quintana, Badillo ann Maldonado (2013) ...
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What is the largest culturable bacterium?
E. coli is easy to grow in the lab. It has a volume around $0.6 \mu m^3$. Source
T. namibiensis can be much larger, with a volume on the order of ${10^6}\mu m^3$. Source
However, T. namibiensis is not ...
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Does heterogeneous resistance have an impact on the zone diameter observed in disk diffusion susceptibility testing?
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Does the presence of small, discrete colonies within the zone of inhibition, suggesting heterogeneous resistance, impact the zone diameter in disk diffusion susceptibility testing?
"Help ...
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Can bacterial DNA solutions, which include other cellular components, be stored without compromising DNA stability?"
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Can bacterial DNA solutions, which include other cellular components, be stored without compromising DNA stability?"
Details
I'm using the boiling extraction method to extract ...
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How is the zone of inhibition formed in disk diffusion?
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How is the zone of inhibition formed in disk diffusion?
What I mean by the formation of the zone of inhibition is not the ultimate visibility of such zone but the circumstances that result in ...
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What's pooling in the spread plate technique?
"Distributing the organisms by rotating the spreader rather than the plate tends to cause more pooling of the inoculum."
https://asm.org/ASM/media/Protocol-Images/Preparing-Spread-Plates-...
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Does inoculum volume affect spread plate outcome?
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The spread plate method is a technique used in microbiology to enumerate and isolate bacteria or other microorganisms present in a sample. It involves evenly spreading a liquid sample ...
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Did operon evolve after endosymbiosis and specialisation of mitochondria?
We know that all extant bacteria use the operon system as their mode of regulating gene expression, in contrast to eukaryotes, which use individual gene promoters as well as epigenetic mechanisms, ...
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Sourcing sulfur-oxidizing bacteria
For a research project, I need a species of sulfur-oxidizing bacteria but I cannot find a biological company that sells anything like that. I know ATCC sells some species of sulfur-oxidizing bacteria ...
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Can placing a culture plate upright introduce contaminants from the lid?
Discussion
I have developed a habit of keeping culture plates always inverted, especially those of non-selective media. This precaution is followed after the poured plate is placed inverted in the ...
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Why did a resazurin broth bottle turn pink immediately after being inoculated?
I have lawn cultures of aerobic bacteria kept at (2-8 °C), which I am trying to revive since they were kept longer than recommended. First, I tried to incubate a broth culture inoculated with a ...
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Is there a way to isolate bacteria from a bacteria/phage plaque in order to PCR bacteria only?
I am writing a paper for a class that involves a mock grant proposal. I am looking at an experiment which requires sequencing of host DNA from a virus/host model, and am having a hard time finding a ...
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Can Gravity convection incubators negatively affect the growth of strict aerobic bacteria?
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Gravity incubators rely on natural convection for heat distribution. There is no fan or blower system, and the heat rises from the heating element at the bottom of the incubator.
https://...
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Can a gene transfer from a plasmid to a bacterial chromosome?
Plasmids are involved in providing resistance to antibiotics. So, can that gene or any other gene transfer from the plasmid to the bacterial chromosome?
Is there any chance where antibiotic resistance ...
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How can Clostridium tetani proliferate in relatively minor wounds?
It is often reported (NIH) that some of the most common infections by Clostridium tetani are in minor wounds where, in theory, blood (hence oxygen) supply should not be completely disrupted.
How can ...
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Storage of bacteria in MgCl2 at 4 degrees
I have a small question to which I am unable to find a proper answer.
Will bacteria in a MgCl2 buffer (or similar solution) at +4 degrees Celsius survive storage for 1-2 days? Or will they die?
What ...
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How can one store non-sterile culture media in dissolved form in the refrigerator?
How do I store non-sterile culture media in dissolved form in the fridge in a clean but non-sterile container?
The powder of the media (Broth, Nutrient agar, MAC, or MH agar) will be put in a glass ...
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How do i create sustainable readily available stock cultures to act as Gram stain controls?
My investigation on the matter
Ref #1
Staining known Gram-positive and Gram-negative organisms on either
side of your unknown organism act as positive controls for your
technique.
(Microbiology ...
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Can a non-pure culture be used for reliable rapid phenotypic diagnostic tests?
Can a non-pure culture be used for reliable rapid phenotypic diagnostic tests (e.g. catalase, oxidase & gram stain)?
"Slide Test Method
Obtain a pure culture of the organism to be tested. ...
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Ames Assay Confusion: Aren't the odds of spontaneous revertants too low to be able to accurately test the mutagenicity of certain compounds?
I am a student conducting a test with the Ames Assay. This assay uses a strain of bacteria that has a mutation in an amino acid synthesizing operon, which doesn't allow it to synthesize its protein. ...
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Why are my bacterial smears disappearing?
I'm trying to inspect simple stained bacterial smears. But my smear suddenly disappears after a successful inspection with the oil immersion lenses.
The background can become too red (the color of the ...
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How do microbiologists decide whether two morphotypes on the same plate belong to the same strain?
Background
Colony: A colony, in microbiology, is a visible cluster or mass of microorganisms (such as bacteria or fungi) that has grown and multiplied on a solid agar medium. Each colony represents a ...
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Is there a reliable method to determine if heat fixation was inadequate before proceeding with further staining steps?
Background
Heat Fixation: Heat fixation is a technique used in bacteriology and other biological sciences to affix bacterial cells or other microorganisms to a microscope slide. This is achieved by ...
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Carbon dioxide concentration in alkaline media
I am running a batch experiment using penicillin bottles where I have bacteria growing for 21 days in a highly alkaline media (pH 10). I am measuring the CO2 and O2 content in the headspace over time, ...
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Does cooking kill all bacteria?
So I know that the general rules is that pathogen prone foods such as chicken shouldn't be consumed after 2 hours at room temperature. Cooking meat such as chicken is necesary as it kills the ...
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Can single-Use Gloves carry bacteria on their outer surface?
In bacteriology, can I touch items (e.g. oxidase discs) with my gloves not fearing to contaminate these items?
Are these gloves (e.g. Disposable nitrile gloves) made so that bacteria can't adhere to ...
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Can I have LAMP on the bench / in the field?
I am interested in LAMP for detecting small amounts of DNA (loop-mediated isothermal amplification and yes, I know the initials don't match).
I am trying to figure out exactly how clean/(sterile?) ...
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What is the mechanism behind antibiotic resistance and how can it be mitigated or prevented? [closed]
What causes antibiotic resistance, and how can we combat it? I am interested in the mechanisms behind antibiotic resistance and strategies to prevent it in bacteria.
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A DNA extraction protocol that is equipment-less for bacterial metagenomes?
I am going into the field soon to sample a very remote environment. For reasons that are unimportant here, I would like to extract bacterial DNA from these samples in the field.
I will have access to ...
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Why do bacteria in a urinary tract infection (UTI) cause bleeding?
It seems that when a urinary tract infection happens, bacteria will cause inflammation to the lining of the urinary tract, and this will cause bleeding.
What is the exact process for the inflammation ...
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Endospores contact with surfaces
I am currently studying endospores-forming bacteria. To be specific B.subtilis. I noticed that the formed spores have strong contact "grip" with the surface it is forming on. For example, ...
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Why isn't bacterial colony a monolayer?
I am an engineer entering the field of microbiology. I was watching bacterial colonies (lab strain E. coli to be specific) being grown on agar plates. I was surprised that the bacterial colony was not ...
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How can a layperson get their hands on a specific strain that's not readily available commercially?
I'm mostly interested to get my hands on L.plantarum D-103, but there are a few other strains as well. D-103 is e.g. mentioned here https://meridian.allenpress.com/jfp/article/80/10/1682/199965/...
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How to figure out growth/culture conditions for a specific strain?
Specifically, I'd like to find out how to grow ME-3 L.Fermentum (DSM 14241) at home without any fancy equipment.
What food does it need? Sugar? Lactose? Something else? At what temperature does it ...
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Methods of denaturing BL21 E.coli other than using lysis buffer and sonication
The goal is to try different denaturing methods for BL21 E.coli. However, finding detailed paper on this is challenging as most articles do not seem to include the details of their denaturing ...
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Superbugs in eusocial animals
I am not a biologist, and I'm interested in eusocial animals, and the porous habitats in which they live. I know few facts about diseases in these animals: I know some interesting instances of viruses,...
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What is the easiest way to determine the species of non-tuberculous mycobacteria?
We have isolated a mycobacterium strain from patient sputum using an NTM Elite plate.
When inoculated into an MGIT tube, we observe fluorescence when observed under UV light, which means it is highly ...
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What's the purpose of using more than one culture medium for bacterial isolation?
What's the purpose of using more than culture medium for bacterial isolation?
Why not use just one?
If this is not generally used in isolation of various types of bacteria, I'm concerned especially ...
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