Questions tagged [bacteriology]

A subdivision of microbiology dedicated to the study of bacteria.

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6
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1answer
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Pink growth (aka pink slime) in bathtub or shower

I was wondering if anybody had studied or was knowledgeable about the pink growth that people often find around water fixtures in their homes, especially bath tubs and showers? My understanding is ...
6
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1answer
2k views

How dirty are bugs? And is it okay if they touch food?

Sometimes I see people throw out an entire drink or bowl of food because a small bug was in it. Are they justified in doing this? Is there enough bacteria on common species of bugs (like ants, flies, ...
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I found this in my refrigerator water filter. Is it a biofilm? Is it a sign of dangerous bacteria?

When I dispense water form my refrigerator after changing my filter, the following slimy tube like deposits appeared. Does anybody know what this is? Could it be a biofilm growing in the water hose, ...
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What disease does Saccharopolyspora erythraea cause?

For an examination assignment I have to find a disease caused by the bacterial species Saccharopolyspora erythraea, but I have searched the internet and have found no report of patients being infected ...
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Can the human gut work with other type of gut bacteria?

I was wondering if the human body would be capable of functioning with, say, cellulolytic bacteria in their gut (instead of or in addition of their current bacteria), like that of ruminants and if ...
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39 views

Can gut flora affect what we can eat and what we cannot?

Back many years ago, I've read a mushroom guidebook, which I don't have anymore. I was unable to find the name of a particular mushroom. The book comprises listing of edible and poisonous mushroom ...
3
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110 views

Do non-pathogenic organism not have PAMPs? Are there any research paper which proves that a certain microbe is non-pathogenic?

According to this PAMPs are delivered along with additional information that can be used by the host to distinguish pathogenic from nonpathogenic microbes and thereby guide the ensuing innate ...
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105 views

Is it inevitable that antibiotics will become useless in the future due to bacteria immunities?

Antibiotics are developed in an ever smaller amount due to the difficulties of discovering new ones. Bacteria, on the other hand, keep "finding" more ways to render antibiotics ineffective, and they ...
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858 views

Can 3% hydrogen peroxide be used as effective disinfectant and antiseptic?

I was curious to see whether 3% hydrogen peroxide can be used as antimicrobial and virucidal product for daily use in home. There are several disinfecting products in the market claim that use of ...
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35 views

How do I test if microbes have survived after dehydration?

I have a solution containing various bacteria and fungi. My aim is to place solution on filter paper, and wait until it dries. I then wish to test if the organisms have survived, either on the dried ...
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57 views

Generate T2/T1 Phage Resistant E Coli

I would like to generate T2/T1 phage resistant Stbl3 E Coli to use in virus production. Is there a plasmid somebody has used that confers resistance, or is this done another way?
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Do sulfate reducing bacteria help during the formation of the mineral dolomite in low temperature environments?

Do sulfate reducing bacteria help during the formation of the mineral dolomite in low temperature environments? Wikipedia says The actual role of bacteria in the low-temperature formation of ...
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How can Haemophilus influenzae survive and grow alone in the human body?

Haemophilus influenzae gets its name from the fact, that it requires nutrients from blood in order to grow. More specifically, I see it mentioned that in vitro they often exhibit the pheomenon that ...
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61 views

Can a plastic degrading bacteria science fair project be recreated in a school lab?

i am just looking for a science fair project and am fascinated by this topic. i was wondering if a project concerning the isolation of plastic degrading bacteria could be done in a school lab. I am in ...
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2k views

What is the difference between enrichment media and selective media?

Enrichment media These media are used to suppress commensal bacteria while allowing the pathogen to remain viable and grow. [Source: Ananthnarayan and Paniker's textbook of Microbiology.] ...
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41 views

Measuring gene similarity in two bacteria models

I'm currently working with some computational models of bacteria. The bacterium I am considering is an Ecoli K-12 which has 139 genes. I have several models of the same bacterium with different ...
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161 views

At what g force do bacteria start to pellet down a tube?

I am working with C. elegans and bacteria and I want to get rid of the bacteria they eat by centrifuging the worms without centrifuging the bacteria. I am using a g-force of 600 and the bacteria ...
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55 views

Would the microgravity “shape-shifting” bacteria effect have anything to do with bacteria growing in a host?

Phys.org's news article Why bacteria 'shapeshift' in space describes a recently published study Phenotypic Changes Exhibited by E. coli Cultured in Space, Zea Luis, Z. et al, 2017, Frontiers in ...
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769 views

Advantages and disadvantages of sporulation compared with competence in bacteria?

Why do bacteria have both of these mechanisms to deal with the same environmental stress: nutrient deprivation? In a culture exposed to this condition, often both competent cells and sporulated cells ...
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146 views

Why did my isolation streak of an unknown organism on Mannitol Salt Agar turn yellow where there was no growth and magenta where there was growth?

I repeated a culture twice in an entry level microbiology class for our final lab project of culturing a provided unknown organism. One of the required tests on our dichotomous key was to culture a ...
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38 views

What is R.ornitholytica? Is it a misspelling of R.ornithinolytica or a different species?

I'm trying to find out which of these is correct, or whether they are different organisms: Raoultella ornithinolytica Raoultella ornitholytica I have come across ...
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35 views

Why are antibacterial body soaps uncommon?

If body odor is caused by bacteria, and antibacterial soap kills bacteria, why aren't antibacterial body soaps hailed as the holy grail of getting rid of body odor? I can only find hand soap that is ...
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310 views

Preserving/disinfecting meat with alcohol?

I'm considering safer ways to handle raw meat and it occurred to me that alcohol might be useful. I'm considering, say, sealing pieces of meat in a container with 40% alcohol or higher in fridge or ...
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30 views

Is it possible to intentionally limit bacteria's lifespan?

Say I have a species of bacteria. I want this species to only persist for a set number of generations, after which it simply stops reproducing. Is it possible to do this at all (with known genetic ...
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61 views

Oxygen Uptake Rates in Microbial Cultures

Normally if air is sparged into a fermenter, the oxygen uptake rate is roughly equivalent to the oxygen transfer rate: KLa(C* - CL) = Xμ/YX/O2. How would this equation change if the oxygen ...
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93 views

How to keep activated sludge viable for a week?

I am working in biological waste water treatment and I need to collect some activated sludge samples and have them characterized through different methods. The characterization will take from a few ...
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30 views

How to track bacteria/ protozoa, unicellular algae in big volumes?

Is there any special cameras and dyes? If I want to track migration/motion of them in 0.1-0.5 cubic metres.
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771 views

Prevent clumping of bacteria in my media so that I can measure turbidity?

Forgive my naivety, I'm an undergraduate Chemical Engineering student doing research this summer into growth rates of various combinations of bacterial isolates. I have about 9 isolates from a toxic ...
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62 views

After a bacterial infection is dealt with, do any of that colony of bacteria remain?

Suppose that I get a bacterial infection, which my immune system deals with. Are there any remnant bacteria, or are they all gone? Now consider the same scenario, but having been treated with a full ...
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102 views

Which Lactobacillales (Lactic acid bacteria — LAB) strains are capable of degrading starch?

I've been researching bacteria strains but am having trouble finding amylolytic LAB strains with amylolytic and lactic acid producing character. The only species I've found, that are capable of ...
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189 views

Effect of flask position in shaking incubator affects growth kinetics

During some cultivation experiments for my bachelor thesis I noticed that in liquid culture my bacteria (E. coli) grew differently, according to the position in the shaking incubator. Because the ...
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98 views

Can two Hfr strains of E. coli conjugate?

Genetics textbooks (and some internet searching) yield abundant examples of Hfr strains conjugating with F- cells, but these sources are surprisingly silent regarding the results of an Hfr ...
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263 views

Transportation of bacteria

I would like to take an apparatus containing bacteria to another country (Bolivia) from the U.S.. How do I determine whether I am allowed to do this? The bacteria are: http://www.atcc.org/Products/...
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493 views

Image Processing Suite for bacterial microscopy: Schnitzcells or MicrobeTracker?

I am looking to start doing some work tracking the size and growth of individual bacterial cells in the microscope. In order to analyze the images I need software that can segment the cells, ...
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16 views

Are controls needed for urine cultures?

If testing for bacteria (gram-negative) in a urine culture, are negative or positive cultures needed? I could not find anything that says controls are needed unless results seem to be contaminated. ...
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23 views

Why is synthesis of tetanospasmin advantageous to C. tetani?

The tetanospasmin is a neurotoxin synthesised by some strains of C. tetani. It is the factor causing tetanus, but what is its role for the bacteria itself? I do not believe the main objective of C. ...
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16 views

official authoritative source for bacterial shape classification

I'm a bit disappointed but after several hours of search, I could not find an official authoritative source for bacterial shape classification. I find a lot, I mean a lot of links but no original ...
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1answer
19 views

Salt Bridge for Microbial Fuel Cell: Full or Partly Full?

I'm making a microbial fuel cell out of benthic mud and salt water in containers. My salt bridge will be made of agar and table salt solidified in PVC pipes. Does the salt bridge connecting the anode (...
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1answer
61 views

Could bacteria and phages be found in tissues?

in the literature, there are several studies of the gut virome and microbiome, for instance Reyes et al. on Nature (https://www.nature.com/articles/nature09199?error=cookies_not_supported&code=...
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What is the role of lactic acid bacteria (such as L. acidophilus and L. bulgaricus) in glycolysis during yogurt fermentation?

I am trying to prove that an increase in monosaccharides (glucose, fructose, galactose) that have been added to milk during fermentation will result in a decreased pH. My current argument is: "The ...
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53 views

How does the symbiotic bacteria reaches a human caecum

My thought on this matter is that we have got a very high pH of about 1.8 in our stomach.So,most of bacteria who came in through food or just open mouth should be killed by it.So,how does a bacterial ...
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1answer
56 views

Home bacteria culture at home?

Can simple bacterial culture be done at home using food agar as the medium (with some additives found in the store)?
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31 views

Can a plastic degrading bacteria project be recreated in a school lab environment?

I am looking for a science fair project and am wondering if this could be a good idea. I am in the 10th grade. I am not exactly sure of the materials needed since this is just the beginning stages of ...
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0answers
76 views

How do the bacteria producing bacteriocins protect themselves from it?

Whenever there is Col plasmid in a bacteria it will produce bacteriocins which will kill other bacteria around it. Why will the bacteriocin not kill the bacteria which has produced it? How does the ...
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506 views

largest and smallest gram positive, gram negative and fungal pathogens

As I stumbled through this question: A 16-year-old female patient presented to her family physician with a complaint of an abnormal vaginal discharge and pruritus (itching). The patient ...
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0answers
61 views

What is the point of inter-species specific quorum-sensing?

So apparently, according to this TED video, there is a kind of quorum sensing involving a universal, inter-species specific molecule that all bacteria secrete. Is there any evidence of bacteria being ...
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94 views

How a biologist attempts to explain the structure of the ribosomal proteins?

I have a Computer Science background and a minimal biology knowledge. This is the question I am asked to answer in one of the biology courses: What would be the structure of ribosomal proteins when ...
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180 views

What are the optimal conditions for storing frozen glycerol stocks of bacteria?

The common method of storing bacteria long-term is to collect saturated liquid culture, such as LB, and mix it with sterile glycerol so as to obtain 10-30% final glycerol concentration. The resulting ...
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What are these colonies on my Bacillus pumilus lawn?

I am growing Bacillus pumilus and I have plated some on agar plates. Now I can see some colonies on top of the lawn. Does anybody know what they could be? A contaminant? Bacillus pumilus? I have ...
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Can C. elegans be raised on Lactobacillus as the only food source?

I'm interested in running some C. elegans experiments for educational purposes, but would like to avoid having to grow or handle E. coli. I was wondering if C. elegans need E. coli specifically, or ...