Questions tagged [bacteriology]

A subdivision of microbiology dedicated to the study of bacteria.

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48 views

Do mutations in one bacterial strain have the same effect in other strains within the same species?

I would assume so, since they are the same species after all. I am asking this because I am doing a bioinformatics project for school and I cannot find any protein mutations for the specific bacterial ...
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2answers
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What placement of plasmid parts makes the most sense?

If you want to express the largest amount of a certain or a few proteins using a plasmid, does it make sense for the antibiotic resistance gene to come after genes for proteins you intend to ...
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How to get sulfate reducing bacteria? [closed]

How can I find and get sulfate-reducing bacteria? Where and how can I store them for a long time?
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Serological assays measuring antibody response

Given that an appropriate immune response to a bacteria may be thwarted in an individual, including not producing all of the antibodies which are known to occur in people who have been infected, or ...
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Examining milk for presence of harmful bacteria

I am not experienced in diagnostic bacteriology and am interested in understanding more about it in the context of analyzing food purity. For example, imagine I have a supply of raw milk which might ...
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1answer
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What if there are no bacteria such as Streptococcus mutans

If someone removed all bacteria such Streptococcus mutans in her mouth by starving long time, or by some chemical method, would she never have such bacteria? In my thought, because S.mutans only ...
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How is bacterial plasma membrane made?

Eukaryotes have ER which manufactures plasma membrane of cells. How is prokaryotic plasma membrane made ? What is the pathway and which enzymes are involved ?
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Gene transfer between Gram positive and Gram negative bacteria?

How does transfer of genetic material occur between Gram positive and Gram negative bacteria?
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What are artifacts in microbiology?

What kind of objects can be qualified as artifacts? How do we distinguish such objects?
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How does temperature impact protein synthesis in E Coli? [closed]

A student wants to study the effect of temperature on protein synthesis in E. Coli. How would you design an experiment to test this out and what results would you expect?
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Is there any description about how microbes spread from a small place to a very large place?

Is there any materials (such as books, papers or others) about how microbes (such as the bacteria or virus) or a special kind of microbe spread from a small place to a very large area (such as the ...
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Is water with 99% total dissolved solids removed conducive to bacteria? [migrated]

Stumbled across Zero Water (https://www.zerowater.com) which claims to remove 99% of total dissolved solids from water. Having read this review (https://wateristhenewgold.com/zero-water-filters-danger-...
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Will ammonia continue to accumulate in an aquarium tank if a strong antibacterial or chlorine is added to the water?

I've always wondered what would happen if dead plants and fecal matter lay in chlorinated waters and/or waters treated with strong antibacterials or antibiotics. I've heard that aquarium tanks ...
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Can I find out what resistance bacteria has based on strain? where do I look for that info?

I have a list of strains across various organisms for example: Escherichia coli - ATCC 25922, CGSC 6152, W3110RL. Pseudomonas aeruginosa - ATCC 27853, PAO1, APAE1111. I'm failing to find any ...
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Optimal bacterial vegetative cell inactivation method(s) after subtilis spores germinated under pressure

I am writing a PhD research proposal and being a newbie in the field I am lost a little bit in the bacterial vegetative cell inactivation methods in foods. I should propose two main methods to my ...
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What are the methods to quantify the bacterial adhesion rate for the biofilms grown on coupons in CDC or annular bioreactor, non-invasively?

I'm interested to know that if I grow the biofilm on coupons in the CDC bioreactor at a particular shear stress, and after say 48 hours, I take out the coupon from the bioreactor to quantify the ...
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What is the protein composition of complete Freund's adjuvant?

The complete form of this adjuvant uses heat inactivated and dried mycobacteria. However, to rule out cross-reactivity with an antibody, I would like to know which proteins are left in the adjuvant. ...
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How antibodies are produced in our body against intracellular proteins of infectious bacteria?

When an infectious agent invades our body, then surface antigens of the infectious agent are detected by our immune system and B-cells get activated. However, we do have antibodies in our blood ...
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Is there a critical size for a bacteria colony forming?

I am a maths student doing a project based on modelling bacteria and phages. In one of my models the bacteria population reaches a population size of ~450 cells. Would a colony of bacteria this size ...
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1answer
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Why doesn't infection with Clostridium Tetani immunize you?

What Immunity to COVID-19 Really Means - Scientific American It is less clear what those antibody tests mean for real life, however, because immunity functions on a continuum. With some pathogens, ...
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I need to identify the genus of this colony just by its morphology, can somebody help me?

it was grown on TSA it's a mesophilic bacteria. (it was contaminated by E. coli, so ignore the clearer and smoother colonies).
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How to prepare bacterin using formalin(formaldehyde)

I am finding how much formalin I have to use to make bacterins for vaccine experiments. I searched for it in google but I can't find about specific procedures like the concentration of formalin and ...
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Can virus and bacteria move on the surface? [duplicate]

Do viruses/bacteria have some motive mechanism? I mean if the virus/bacteria reach some surface by touching, can it move on that surface? Life example: suppose the cooler is not so clean and has ...
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How to convert AU/ml of bacteriocin into zone of inhibition in mm?

My questions is about measuring antimicrobial activity against bacteria on petri plate. Is it possible to convert or somehow get zone of inhibition in millimetre from AU/ml?
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Salt water swish for dental health [duplicate]

I was wondering as to why warm water is told for salt water gargle and not cold water, does warm water has more therapeutic effect? Also does salt really work, excess salty water might kill bacteria ...
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From a hosts point of view, which comes first, effect of toxins on pathways or identification of toxins by immune system?

From a pure biology perspective, when a pathogen (gram-negative bacteria) affects a host by secreting toxins, what comes first, the effect of the toxin on the pathways or identification of the toxin ...
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Is bacteria capable to learn?

Are bacteria chemicaly guided or there is some kind of complex electronic system that let them learn the envirement?Thanks.
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How can I propagate a community of anaerobic bacteria sampled from mud in a bog?

I'm doing research on anaerobic bacteria. In the fall I took mud samples from a bog and have been working with them but I didn't take enough. Now winter has rolled around (Im in Canada) and all the ...
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Could Cyanobacteria farms help dilute pollutants in the atmosphere

If I understand correctly, roughly ~2.8 billion years ago cyanobacteria started pumping large amounts of oxygen into the atmosphere. Using modern industrial processes could this be emulated by ...
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Ideas for microbiology lab. Antimicrobial properties of cutting boards

I am an undergraduate student in Biology and I am trying to design a microbiology lab that will takes 3 weeks to carry out (1-2 day each week). I am going to examine the antimicrobial properties of ...
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Scientifically testing when milk goes bad

How could I test if milk has gone bad scientifically? Performing a taste test on the milk yields a subjective interpretation as to whether the milk is good or not. Is there any objective metric that I ...
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1answer
48 views

How big can a colony/society get?

I hope this is the appropiate place for this question, asked from a math/physics perspective with little to no training in biology. Bacteria are very small and live in huge colonies, ants are bigger (...
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Are most pathogenic bacteria facultative anaerobes?

I know that S. aureus and most gram-negative rods are facultative anaerobes. In terms of number of species, are most human pathogen-associated bacteria facultative anaerobes?
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How many types of bacteria?

Bacteria are classifed on the base of food. Two types of bacteria, autotrophic and heterotrophic bacteria. Autotrophic bacteria that prepare own food or energy. Heterotrophic which cannot prepare own ...
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E. coli & aspartate

I am familiar with the linkage between aspartate/ligand binding, receptor methylation, and flagellar behavior re. style of locamotion, but I do not know what the bacterium does with the aspartate it ...
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What enzyme or bacteria could dissolve plastic?

What enzyme or bacteria could dissolve plastic aside from acetone? Preferably, low-cost and environmentally friendly. Any suggestions would be helpful. I'm targeting for thin plastic (low-density ...
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1answer
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Immune response to IgA positive bacteria

If certain bacteria can be coated with IgA in vitro, does that mean they are likely to elicit an IgA immune response? Edit I'm working on a project that involves IgA-Seq analysis. Bacteria are coated ...
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Size distribution of all known species in the domain bacteria

I have found the smallest bacteria are around 200nm (e.g Mycoplasma genitalium an ultramicrobacterium) and largest are around 700um (Thiomargarita namibiensis). I am looking for a graph that ...
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How do I homogenise a small pellet of 10 to 50 cells within less than 50 µL?

I have a problem with declumping/ homogenising bacterial cells in small volumes (10 to 50 µL). I know that I can get all of my cells out of the tube, but they are still stuck together and won't form ...
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What is a secretion target family protein?

I came across this link: https://www.genome.jp/dbget-bin/www_bget?btw:BF38_3398 What is this protein, is it a secreted effector protein or is it something else? What is the difference between type ...
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Is there a glossary about which infrared (IR) colors which body parts reflect?

I am doing medical research and I'm trying to find parts of the body that look about the same to the human eye, but reflect different "colors" in IR, so that using a light source and a camera of that ...
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How would infection of mosquitos with sterilizing bacteria have any effect?

According to this article from the New York Post, the US just approved the mass release of male mosquitos infected with a bacteria that sterilizes them. The idea, according to the article and some ...
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Role of non-inflammation in H.Pylori positive people

Quoted from Saudi J Gastroenterol. 2009 Jul; 15(3): 201–207 publication : Approximately 50% (over 3 billion) of the world populations are known to be infected with Helicobacter pylori, mainly in ...
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Using Transduction to reverse Antibiotic Resistance?

Is it possible to use reverse transduction to reverse antibiotic resistance. Since antibitiotic resistance is causee by transduction of the F factor, is it possible to induce a F+ non antibiotic ...
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1answer
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What does it take to start producing(cultivating) probiotics?

Is there a DIY way? Is it possible to make a bacteria strain from scratch based on an existing one ? (for example lactobacillus reuteri).
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what are the ethical considerations when preparing a bacterial culture for testing antibiotic resistance? [closed]

I cannot think of any ethical issues associated with preparing a bacterial culture to test antibiotic resistance. The bacteria I am using is Bacillus Megaterium so its not pathogenic. Thanks in ...
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Are antibiotic-resistant bacteria necessarily less virulent than their original strain?

In the absence of antibiotic treatment, is there a reduction in the average severity of disease caused by resistant strains of pathogenic bacteria compared to wild-type ones? I would assume that ...
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Do plant and animal bacterial pathogen differ too much?

I will be involved in a plant disease produced by a bacterial pathogen, Acidovorax citrulli. I've previously worked with animal pathogens such as Haemophilus parasuis. Are there any big differences ...
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Need clarification on a sentence regarding vesicle-mediated translocation

I've chanced upon a passage that is not entirely clear to me: In addition to conjugation, transformation and transduction, other less well recognised mechanisms of DNA uptake occur in nature, while ...
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What's the composition of Actinobacteria's hyphae?

I'd like to know which chemical compounds form hyphae in Actinobacteria. Is it the same structure of the cell wall? Thank you

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