Questions tagged [bacteriology]

A subdivision of microbiology dedicated to the study of bacteria.

Filter by
Sorted by
Tagged with
7
votes
2answers
580 views

How do infectious bacteria determine when their numbers are high enough to attack a host?

When you get sick, you generally don't contract enough bacteria at once for them to succeed in battling your immune system, right? Their numbers must gradually increase in the host's body before they ...
7
votes
3answers
2k views

How quickly is antibiotic resistance lost?

I would imagine the bacterial genome is highly conserved and limited in its space, but maybe I am wrong. If you were to take a strain of antibiotic resistant bacteria and kept them isolated, but fed ...
7
votes
4answers
601 views

How to measure bacterial content

I´m using water container placed on a radiator to humidify room air. Someone might argue that it is a perfect nest for bacteria. I have to put copper coins into the water to kill the bacteria. I ...
7
votes
2answers
1k views

Does soap kill human cells?

I see many products, particularly hand soap and cleaning products, that claim to kill 99.9% or more of bacteria. This makes me wonder, if the chemicals are potent enough to break down bacterial cell ...
7
votes
2answers
636 views

Why does botulinum toxin seem to be more dangerous to humans than to other mammals?

Various mammals seem to get away with eating parts of carcasses that we would prefer to not even touch, and that we assume will make us sick. Because of that, I assume botulinum toxin is more ...
7
votes
1answer
426 views

How much of the weight of yoghurt is due to bacteria?

I recently started making all kinds of bacteria-processed foods by myself (such as yoghurt, but also Sauerteig and so on), and I found myself wondering how much of the weight of these products is ...
7
votes
1answer
2k views

Why don't we build up an immunity to sore throat?

We often get sore throats once or twice a year, and it clears in a few days sometimes without any antibiotics. I was wondering why doesn't our body become immune after clearing a sore throat?
7
votes
2answers
1k views

Alternatives to CFU plating for measuring number of viable cells?

I am hoping to measure growth rates of a bacterial culture in several growth conditions. I am concerned that these growth conditions may cause cell death, which would lead to a decreased ...
7
votes
2answers
3k views

Bacterial cell lysis - what solution to use?

I am trying to determine how quickly detergents act on bacterial cells (cell lysis). I would like to compare some detergents at difference concentrations for bacteriolytic activity. I don’t care about ...
7
votes
1answer
2k views

Why don't bacterial cell walls prevent bursting when attacked by the complement?

The complement system creates pores in cell membrane which leads to influx of lots of water thereby causing lysis of bacterial cell. But what I fail to understand is that if bacteria have cell walls ...
7
votes
2answers
180 views

Would current-day human-initiated panspermia be effective?

With current technology, we are able to easily send a probe to the Alpha Centauri star system at a speed of about 20 km/s (velocity of voyager probe), which means it would take about 65000 years to ...
7
votes
1answer
341 views

Is there a detectable amount of bacterial DNA in the blood of infected persons?

With which bacterial infection in humans has it been shown that bacterial DNA can be found in the blood? If any is found it is likely not to be very much, and even difficult to distinguish from ...
7
votes
3answers
362 views

Can Helicobacter pylori be eradicated naturally?

This question is not to discuss whether substances that have anti-H. pylori activity work or not, or to discuss the pros and cons of such but from a medical perspective. Assuming that one is able to ...
7
votes
1answer
111 views

Are there mechanisms that limit the amounts of time conjugation and F-plasmid transfer can happen? If yes, what are they?

Not all bacteria have acquired F-plasmids through conjugation. Some of the mechanisms for this are unsuccesful conjugation events (mechanical disruption), no transfer due to integration in bacterial ...
7
votes
1answer
1k views

How is the exogenous DNA protected from degradation during bacterial transformation?

During transformation, a bacterium can take up DNA from its environment. A small fraction of bacterial species are known to be naturally competent, meaning that they can engage in this sort of ...
6
votes
2answers
16k views

Why do different bacteria have different shapes?

Why do different bacteria have different shapes? Is it only related to their function?
6
votes
1answer
1k views

Why do some bacteria have most genes on the leading strand of the genome?

Genes in the (+) strand are black and genes in the (-)strand are red. The gene distribution in E. coli genome is somewhat expected: transcribed regions would tend to alternate with non transcribed ...
6
votes
2answers
315 views

Bacteria trapped in crystal inclusions found 'alive' after 50,000 years - what were they eating all that time?

The phys.org article Biologists find weird cave life that may be 50,000 years old describes the announcement by NASA Astrobiology Institute director Penelope Boston at the 2017 AAAS meeting$^{(1)}$ of ...
6
votes
2answers
2k views

Can Hepatitis be caused by Bacteria?

There are many information about viral hepatitis. But it is interesting for me, can bacteria cause the hepatitis too?
6
votes
2answers
12k views

How do I measure bacterial growth in agar dishes (either by cell mass or by cell count)?

I am doing an experiment in which I am growing S. mutans in agar dishes, and I am not sure how I would measure the growth of the S. mutans. I am also not sure if I would do this by measuring cell mass ...
6
votes
1answer
4k views

Does every wave length of UV kill bacteria?

Will every wave length of UV work for killing bacteria? So will UV A, UV B and UV C all work? Or will only the shortest of the three, UV C, work? Also, will the average UV lamp help against bacterial ...
6
votes
1answer
114 views

Do free computer programs exist that can simulate drug permeability in bacterial membranes?

I am currently trying to make derivatives of a molecule in hopes of making it more permeable to bacterial membranes. I wanted to know whether there are any bacterial membrane simulating programs where ...
6
votes
1answer
29k views

How does heat shock transformation work?

What exactly happens when competent cells like DH5ɑ are heatshocked with DNA present? How does the DNA get inside the cells? Specifically, why are all the steps necessary? What if you heatshock right ...
6
votes
1answer
543 views

How can promoter binding sites be determined?

I have been trying to find out which sigma factor is responsible for the transcription of RNA polymerase subunits $\alpha$ (rpoA) and $\beta ^{\prime}$ (rpoC) in Bacillus subtilis. I would expect it ...
6
votes
1answer
416 views

Whole genome amino acid composition tool?

I'm interested in a statistical tool to get bacterial codon usage at genomic level. Ideally, the tool should be flexible to analyse hundreds of bacterial genomes. I've looked in MeSH terms database ...
6
votes
1answer
2k views

Can bacteria, viruses, and cancer cells be destroyed by resonance?

Radiotherapy has been used to treat cancer. Can the resonances by coordinated electromagnetic waves (and/or other forms of waves), of various frequencies, amplitudes and pulse rates, directed from ...
6
votes
1answer
241 views

What is the advantage of using plant-derived antibacterials rather than bacteria-derived antibacterials?

So obviously we have a big problem with antibiotic resistance. Most of our antibiotics originate from bacteria themselves (or are synthetic variations on scaffolds which originate from bacteria). I ...
6
votes
1answer
111 views

Proteins that are strongly overproduced in E. coli and S. cerevisiae?

I'm looking for some pointers to proteins that produce at really gigantic levels in E. coli and yeast (S. cerevisiae). Can anyone point to some champion proteins? Even in inclusion bodies and non ...
6
votes
1answer
202 views

Mutation-immunity in Luria-Delbruck experiment

If experiments like those of Luria and Delbruck on E. coli and T1 phage are the main source of our confidence in the mutation-immunity model, is it then highly unlikely that there are other types of ...
6
votes
1answer
163 views

What sort of biofiltration is best for a small, portable, biofiltration unit?

I've been exploring the idea of a system to allow re-use (drinking) of RV greywater, or at least the freedom to dump in storm drains (I think the requirements are looser about this, but it still has ...
6
votes
1answer
276 views

Conjugation in bacteria

I was studying about conjugation in bacteria, I have a doubt. In conjugation F- cell transforms into F+ cell when there is a formation of sex pilli in between the two. The doubt is if the F- cells ...
6
votes
1answer
133 views

Is 1 g/l living biomass for a Biogas fermenter plausible?

A usual number for a healthy biogas fermenter is $10^9 - 10^{10}/mL$ Bacteria, 10-15% of which would be methanogenic archae. Exceptionally healthy fermenters have more total bacteria and up 25% archae....
6
votes
1answer
2k views

Pink growth (aka pink slime) in bathtub or shower

I was wondering if anybody had studied or was knowledgeable about the pink growth that people often find around water fixtures in their homes, especially bath tubs and showers? My understanding is ...
6
votes
1answer
2k views

How dirty are bugs? And is it okay if they touch food?

Sometimes I see people throw out an entire drink or bowl of food because a small bug was in it. Are they justified in doing this? Is there enough bacteria on common species of bugs (like ants, flies, ...
5
votes
1answer
453 views

Approximately how long do bacteria live for? [closed]

I would like to know how many days or years do the bacteria live approximately.
5
votes
4answers
3k views

What is the advantage of restriction enzymes cutting only at specific sites?

Bacteriophages have sequences which often do not have specific sites for restriction enzymes of bacteria to cut at and so can attack the bacteria. Wouldn't it be better if bacteria had something "...
5
votes
2answers
430 views

How to identify genes required for biofilm formation

This is just a homework question: Q: Let’s assume that there is an unknown set of E. coli genes that are required for biofilm formation. Describe a genetic experiment you could perform to try to ...
5
votes
1answer
2k views

Can I kill bacteria with distilled water?

Given that bacteria are hypertonic (contain more salts) compared to distilled water, and that they rely on ion concentration differences across the plasma membrane to survive, can I kill a bacterial ...
5
votes
2answers
286 views

Gas from bacteria that's not methane

Is gas produced by bacteria always mainly methane? Or, are there bacteria out there that produce some biogas composed mainly of hydrogen, natural gas, propane, butane?
5
votes
4answers
2k views

Can general soap kill bacteria?

I have read that general soap can kill bacteria by opening holes in the bacterial membrane. http://questions.sci-toys.com/node/90 However, I found some articles as well saying that it cannot. http:/...
5
votes
1answer
49k views

Why are bacteria and archaea in different domains?

As I understand it, the main difference between the Bacteria and the Eucaryota domains are that eukaryotes have a nucleus and bacteria don't. I understand that bacteria and archaebacteria have enough ...
5
votes
2answers
122 views

why don't antibiotics reduce our weight?

I know that a significant weight of the human body (in Kgs) is contributed by microbiota. I also know that antibiotics can often be broad spectrum and kill all the bacterias regardless of them being ...
5
votes
3answers
4k views

What is the mechanism of oxygen uptake in E. coli?

How does E. coli uptake oxygen? Most of the literature I found is concerned with response to oxygen level supplied in the medium, as opposed to how much is actually transported inside. Can they shut ...
5
votes
1answer
28k views

Calculation of the bacterial growth rate from a spectrophotomer growth curve

Typically the microbial growth in liquid cultures is monitored by turbidity. Data is obtained with a spectrophotometer to measure optical density at 600nm. The slope of the bacterial kinetic curve in ...
5
votes
2answers
20k views

Does drinking alcohol having UTI may help to kill the bacteria?

It's well known that ethyl alcohol kills bacteria. And amount of alcohol in urine may be detected by EtG tests (as long as 3-4 days after intake). Does drinking alcohol in reasonable amount by ...
5
votes
1answer
7k views

What is the distinction between F' plasmid and R plasmid?

Is there a difference between an F' plasmid that has taken up a chromosomal gene that conveys antibiotic resistance, and an R plasmid? Is a bacterium containing an R plasmid and yet lacking an F+ ...
5
votes
1answer
99 views

Why don't phagocytes eliminate mutualistic foregin organisms residing in our body?

Many organisms residing in our body and have a symbiotic mutualistic relationship with our body e.g. organisms in our small intestines. How come our body does not activate an immune response against ...
5
votes
1answer
784 views

Can bacteria or other microorganisms be killed by applying pressure?

Can you smash a bacteria (or any microorganism) and kill it? Let's say there is millions of microorganisms on a table. When you put your finger on the table, will or can you kill at least some of them ...
5
votes
1answer
2k views

Why don't all bacteria have F-plasmids by now?

Some bacteria can undergo gene transfer by conjugation. Conjugation is a form of horizontal gene transfer, meaning from one (unrelated) bacterium to another (in contrast to vertical gene transfer, ...
5
votes
1answer
318 views

Why is E.coli used as a model?

Is there a reason for the choice of E.coli as a model for many bacterial systems? Other bacteria such as B.subtilis are also used, but why is E. coli preferred?