Questions tagged [bacteriology]

A subdivision of microbiology dedicated to the study of bacteria.

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104 views

Extract bacteria from compost?

I'm working on a project where I need to find certain cellulolytic bacteria. I was looking at this list : http://webcache.googleusercontent.com/search?q=cache:CrtQ9T6K7m8J:www.wzw.tum.de/mbiotec/...
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1answer
867 views

What happens to Bacteria at a low pH?

I am conducting an experiment on how different pH levels effect the growth of E.coli. After researching, it is predicted that the most acidic pH level (pH 2) will be most effective at killing (or at ...
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1answer
103 views

How much does sprayed water remove bacteria?

I was quite skeptical about some company claiming that their device was able to suppress 99.99 % of bacteria. Then I read a report testing the germicidal effect of their device, from a large and ...
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1answer
225 views

Lactobacillales vs LAB (Lactic acid bacteria)

As far as I understand bacteria of order Lactobacillales are all LAB (Lactic acid bacteria). So often "Lactobacillales" and "LAB" are used interchangeably. But I also thought that this is not the ...
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1answer
754 views

Can different bacteria strains of same species have different genomic sizes?

Can two different strains of a single species have different genomic sizes? I am not asking about plasmid. Can they have different sizes of actual chromosome?
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1answer
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Survival of streptococcus after my scarlet fever

I'm currently sick because of scarlet fever but I got treated with antibiotics. I know that after 1.5 day using antibiotics the streptococci I release at home are dangerous anymore. But my question is,...
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1answer
2k views

How does sucrose protect bacterial cells in lysozyme solutions?

I have a microbiology question. When we put bacterial cells in sucrose solution with concentration higher than 0.5M we observe plasmolysis - the cytoplasmic membrane detaches from the cell wall due ...
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1answer
188 views

What is the film that covers the tongue?

What is the film that covers the tongue in the mornings, even after brushing the teeth and tongue the night before and why does it have color variations? Do the different colors mean anything?
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128 views

Speciation and Phylogeny of Lactobacillus

The lactobacillus, also called Döderlein's bacillus is a genus of facultative aerobic bacteria. There are several species such as Lactobacillus acidophillus and Lactobacillus reuteri. I have several ...
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1answer
208 views

Is it possible to make anaerobic media too reducing?

I've been struggling to grow an anaerobe (P. gingivalis), I take every precaution to keep the culture conditions free of oxygen, I was wondering if the conditions can be so reducing that they inhibit ...
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2answers
423 views

What culture medium should we use for tap/drinking water bacteria?

We are a group of high school students from Manila who is performing an investigatory project on a topic that we chose which, at first glance, seemed simple, but turned out a bit complicated. We're ...
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1answer
54 views

Are bacterial RNA polymerases distributed evenly throughout the cell or localized?

Bacterial cells aren't internally compartmentalized with membranes (like eukaryotes). This naturally leads to an image of a homogeneous interior, but bacterial cytoplasm isn't homogeneous. Case in ...
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How to feed bacteria out of lab

I want to take a sample of skin infection bacteria (I suppose combination of staphylococus, strep, etc) and give it to the doctor for analysis but the appointment is in two weeks. The bacteria will be ...
3
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3answers
162 views

How much genomic variation one usually find within a given bacterial species?

If I find the exact starting position (say 1152471) of the coding sequence of a given gene in the genome of a bacterium, is the genome of the bacterium in general stable enough so that I can expect to ...
3
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1answer
551 views

Regulation of V. cholerae virulence factors

So I know that several different environmental signals, such as pH, bile, and temperature, regulate virulence gene expression in V. cholerae. Specifically, they control expression of the genes ...
3
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1answer
213 views

Horizontal Gene Transfer

I understand the different ways bacteria can undergo horizontal gene transfer (transformation, transduction (phages), conjugation (plasmids)). Is there an experimental method to tell how a specific ...
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1answer
2k views

Does Pseudomonas aeruginosa have any common name at all?

Pseudomonas aeruginosa is the fifth most written about¹ binomial name of all species. I'm having trouble coming to terms with the possibility that such a well researched and studied organism has never ...
3
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1answer
292 views

Could the growth rates of Vibrio vulnificus be affected by an oil spill?

I live on the Gulf Coast, and Vibrio vulnificus has recently made the news due to an increase in the number of reported infections. Naturally, it has people around here worried. I live in an area that ...
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1answer
51 views

Do sulfate reducing bacteria help during the formation of the mineral dolomite in low temperature environments?

Do sulfate reducing bacteria help during the formation of the mineral dolomite in low temperature environments? Wikipedia says The actual role of bacteria in the low-temperature formation of ...
3
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1answer
394 views

Are the intestinal microbiota substantially different within a diverticulum of the colon?

From the Wikipedia page for the vermiform appendix: This proposal is based on a new understanding of how the immune system supports the growth of beneficial intestinal bacteria, in combination with ...
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78 views

How can Haemophilus influenzae survive and grow alone in the human body?

Haemophilus influenzae gets its name from the fact, that it requires nutrients from blood in order to grow. More specifically, I see it mentioned that in vitro they often exhibit the pheomenon that ...
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66 views

What disease does Saccharopolyspora erythraea cause?

For an examination assignment I have to find a disease caused by the bacterial species Saccharopolyspora erythraea, but I have searched the internet and have found no report of patients being infected ...
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41 views

Can the human gut work with other type of gut bacteria?

I was wondering if the human body would be capable of functioning with, say, cellulolytic bacteria in their gut (instead of or in addition of their current bacteria), like that of ruminants and if ...
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Can gut flora affect what we can eat and what we cannot?

Back many years ago, I've read a mushroom guidebook, which I don't have anymore. I was unable to find the name of a particular mushroom. The book comprises listing of edible and poisonous mushroom ...
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1answer
101 views

Where can I find a compendium of microorganisms?

I recently bought a microscope hoping to learn more about algae and bacteria and stuff. I've collected a few samples of pond water and found tons of algae and stuff, but I have no idea what to call ...
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123 views

Do non-pathogenic organism not have PAMPs? Are there any research paper which proves that a certain microbe is non-pathogenic?

According to this PAMPs are delivered along with additional information that can be used by the host to distinguish pathogenic from nonpathogenic microbes and thereby guide the ensuing innate ...
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109 views

Is it inevitable that antibiotics will become useless in the future due to bacteria immunities?

Antibiotics are developed in an ever smaller amount due to the difficulties of discovering new ones. Bacteria, on the other hand, keep "finding" more ways to render antibiotics ineffective, and they ...
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56 views

Mechanistically, how does calcium chloride aid in bacterial transformation? [duplicate]

I am looking to get more information about the mechanism of the $CaCl_2$ in the bacterial transformation. I read online about the topic but I didn't found any reverent information on the topic, I don'...
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2k views

Can 3% hydrogen peroxide be used as effective disinfectant and antiseptic?

I was curious to see whether 3% hydrogen peroxide can be used as antimicrobial and virucidal product for daily use in home. There are several disinfecting products in the market claim that use of ...
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35 views

How do I test if microbes have survived after dehydration?

I have a solution containing various bacteria and fungi. My aim is to place solution on filter paper, and wait until it dries. I then wish to test if the organisms have survived, either on the dried ...
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60 views

Generate T2/T1 Phage Resistant E Coli

I would like to generate T2/T1 phage resistant Stbl3 E Coli to use in virus production. Is there a plasmid somebody has used that confers resistance, or is this done another way?
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3answers
2k views

Is there a specific word for bacterial death?

What do you call it when a bacterium dies? Cellular death is apoptosis, necrosis, and bacterial is ...? I don't simply want to write in a paper that it - well dies!
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1answer
104 views

Which bacteria takes the longest to double?

Currently I am writing a lab report on the enumeration of bacteria. E.coli takes about 30 mins to double. That is going from 1 bacteria to 2 bacteria. I want to know which bacteria takes the ...
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4answers
2k views

Do I need to serially dilute E. coli cultures for optical density measures?

I am expected to track cell growth by measuring the incubating culture's cell concentration every 30 minutes or so. So my questions are: Why do I need to do serial dilution (assuming that I do)? Why ...
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2answers
155 views

Why isn't Bubonic Plague as virulent as it once was?

Although I am admittedly not anywhere near an expert on any topic, I still have a hard time understanding how Black Death and Bubonic plague are one in the same. Since plague still exists, with news ...
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1answer
2k views

Why does ampicillin in solution turn yellow?

I have a universal tube with 10 mg mL-1 ampicillin. When I got it, it was supposed to be sterile. It was opened for approximately 20 minutes for an experiment and has since been standing around sealed ...
2
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2answers
1k views

What are the (correct) terms for these flagella-arrangements?

I came across the following diagrams depicting two kinds of arrangement of flagella over cells. Now according to my (very unreliable) school textbook, the arrangements are termed as: A - ...
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1answer
79 views

Have bacteria evolved antibiotic resistance in a laboratory setting?

Is it possible to breed a strain of bacteria in a laboratory setting so that it gains resistance to an antibiotic, replicating the kinds of resistance seen in wild, and has that been done?
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3answers
9k views

How do viruses or bacteria survive outside the body long enough to spread?

Say I cough on my table, then someone else touches it and picks up something I've got... how is it that these things can live outside the body, how long can they manage it, and how long is generally '...
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1answer
391 views

List of all reported bacteria

I would like to know were to download a table file with all reported bacteria and its corresponding PHYLUM, CLASS, ORDER and FAMILY. Basically, its the same table than this one from GOLD genomes but ...
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1answer
464 views

What constitutes as the death of a bacteria?

I've read that some bacteria can survive a long time without food. What constitutes the death of a bacteria ? What process needs to take place for it to be considered dead?
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1answer
2k views

Do lysogenic viruses only infect bacteria?

In the video sent by my teacher on viruses, the example he used for lysogenic viruses was a bacteriophage infecting a bacteria. When he was describing how the genetic material was incorporated into ...
2
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1answer
59 views

Why is it possible to make yoghourt with lactose-free milk?

The function of the Lactobacillus bulgaricus and Streptococcus thermophilus cultures is to ferment lactose to produce lactic acid. The increase in lactic acid decreases pH and causes the milk to clot,...
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1answer
120 views

Commensalism with Virus bacteria [duplicate]

Why people die of infection from microorganism, when germs can keep breeding for longer if they dont kill host? Would not millions of years of evolution make germs evolve to not to kill human but feed ...
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1answer
538 views

Role of dam methylase in bacteria

In bacteria, an enzyme called dam methylase (Deoxyadenosine methylase) methylates adenines (A) in the sequence GATC in the new strand formed after replication. What role does this methylation play? ...
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2answers
952 views

Cleavage of RNA by restriction enzymes?

Six restriction enzymes discussed in Sequence-specific cleavage of RNA by Type II restriction enzymes (Murray et al.) have the ability to detect and cut RNA strands with that enzyme's recognition ...
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1answer
137 views

Is [O2] different in a 5 mL liquid media?

Is there a drastic difference between top and bottom of the tube, with no stirring?
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1answer
114 views

Aside from cockroaches and other insects, which species (of all kinds) are 'immune' to ionizing radiation?

I have just been reading this excellent question and answer about cockroaches ability to withstand ionizing radiation. My question is an extension of that question: aside from insects, which other ...
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1answer
65 views

Can viruses stabilize and increase the shelf life of wine and other foods?

When you make wine, you use the yeast Saccharomyces cerevisiae to ferment sugary juices. After you're done, you want to avoid them doing anything by using some chemicals etc. There are also other ...
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1answer
101 views

Loss of antibiotic resistance due to long term storage

How possible is it for antibiotic resistant bacteria stored for a long time to lose such resistance?

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