Questions tagged [bacteriology]

A subdivision of microbiology dedicated to the study of bacteria.

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1answer
207 views

Horizontal Gene Transfer

I understand the different ways bacteria can undergo horizontal gene transfer (transformation, transduction (phages), conjugation (plasmids)). Is there an experimental method to tell how a specific ...
3
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283 views

Could the growth rates of Vibrio vulnificus be affected by an oil spill?

I live on the Gulf Coast, and Vibrio vulnificus has recently made the news due to an increase in the number of reported infections. Naturally, it has people around here worried. I live in an area that ...
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386 views

Are the intestinal microbiota substantially different within a diverticulum of the colon?

From the Wikipedia page for the vermiform appendix: This proposal is based on a new understanding of how the immune system supports the growth of beneficial intestinal bacteria, in combination with ...
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What disease does Saccharopolyspora erythraea cause?

For an examination assignment I have to find a disease caused by the bacterial species Saccharopolyspora erythraea, but I have searched the internet and have found no report of patients being infected ...
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Can the human gut work with other type of gut bacteria?

I was wondering if the human body would be capable of functioning with, say, cellulolytic bacteria in their gut (instead of or in addition of their current bacteria), like that of ruminants and if ...
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Can gut flora affect what we can eat and what we cannot?

Back many years ago, I've read a mushroom guidebook, which I don't have anymore. I was unable to find the name of a particular mushroom. The book comprises listing of edible and poisonous mushroom ...
3
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93 views

Where can I find a compendium of microorganisms?

I recently bought a microscope hoping to learn more about algae and bacteria and stuff. I've collected a few samples of pond water and found tons of algae and stuff, but I have no idea what to call ...
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Do non-pathogenic organism not have PAMPs? Are there any research paper which proves that a certain microbe is non-pathogenic?

According to this PAMPs are delivered along with additional information that can be used by the host to distinguish pathogenic from nonpathogenic microbes and thereby guide the ensuing innate ...
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105 views

Is it inevitable that antibiotics will become useless in the future due to bacteria immunities?

Antibiotics are developed in an ever smaller amount due to the difficulties of discovering new ones. Bacteria, on the other hand, keep "finding" more ways to render antibiotics ineffective, and they ...
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55 views

Mechanistically, how does calcium chloride aid in bacterial transformation? [duplicate]

I am looking to get more information about the mechanism of the $CaCl_2$ in the bacterial transformation. I read online about the topic but I didn't found any reverent information on the topic, I don'...
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858 views

Can 3% hydrogen peroxide be used as effective disinfectant and antiseptic?

I was curious to see whether 3% hydrogen peroxide can be used as antimicrobial and virucidal product for daily use in home. There are several disinfecting products in the market claim that use of ...
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35 views

How do I test if microbes have survived after dehydration?

I have a solution containing various bacteria and fungi. My aim is to place solution on filter paper, and wait until it dries. I then wish to test if the organisms have survived, either on the dried ...
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Generate T2/T1 Phage Resistant E Coli

I would like to generate T2/T1 phage resistant Stbl3 E Coli to use in virus production. Is there a plasmid somebody has used that confers resistance, or is this done another way?
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Do sulfate reducing bacteria help during the formation of the mineral dolomite in low temperature environments?

Do sulfate reducing bacteria help during the formation of the mineral dolomite in low temperature environments? Wikipedia says The actual role of bacteria in the low-temperature formation of ...
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3answers
2k views

Is there a specific word for bacterial death?

What do you call it when a bacterium dies? Cellular death is apoptosis, necrosis, and bacterial is ...? I don't simply want to write in a paper that it - well dies!
2
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1answer
73 views

Which bacteria takes the longest to double?

Currently I am writing a lab report on the enumeration of bacteria. E.coli takes about 30 mins to double. That is going from 1 bacteria to 2 bacteria. I want to know which bacteria takes the ...
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4answers
2k views

Do I need to serially dilute E. coli cultures for optical density measures?

I am expected to track cell growth by measuring the incubating culture's cell concentration every 30 minutes or so. So my questions are: Why do I need to do serial dilution (assuming that I do)? Why ...
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2answers
137 views

Why isn't Bubonic Plague as virulent as it once was?

Although I am admittedly not anywhere near an expert on any topic, I still have a hard time understanding how Black Death and Bubonic plague are one in the same. Since plague still exists, with news ...
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1answer
2k views

Why does ampicillin in solution turn yellow?

I have a universal tube with 10 mg mL-1 ampicillin. When I got it, it was supposed to be sterile. It was opened for approximately 20 minutes for an experiment and has since been standing around sealed ...
2
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2answers
988 views

What are the (correct) terms for these flagella-arrangements?

I came across the following diagrams depicting two kinds of arrangement of flagella over cells. Now according to my (very unreliable) school textbook, the arrangements are termed as: A - ...
2
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3answers
508 views

Could plasmids and conjugation mechanisms be used against antibiotic-resistant bacteria? [closed]

I'm surprised no one has mentioned something like this. Plasmids are often exchanged between bacteria, sometimes through conjugation. In particular, conjugation could be considered an "open-port" ...
2
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1answer
76 views

Have bacteria evolved antibiotic resistance in a laboratory setting?

Is it possible to breed a strain of bacteria in a laboratory setting so that it gains resistance to an antibiotic, replicating the kinds of resistance seen in wild, and has that been done?
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3answers
8k views

How do viruses or bacteria survive outside the body long enough to spread?

Say I cough on my table, then someone else touches it and picks up something I've got... how is it that these things can live outside the body, how long can they manage it, and how long is generally '...
2
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1answer
386 views

List of all reported bacteria

I would like to know were to download a table file with all reported bacteria and its corresponding PHYLUM, CLASS, ORDER and FAMILY. Basically, its the same table than this one from GOLD genomes but ...
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452 views

What constitutes as the death of a bacteria?

I've read that some bacteria can survive a long time without food. What constitutes the death of a bacteria ? What process needs to take place for it to be considered dead?
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1answer
2k views

Do lysogenic viruses only infect bacteria?

In the video sent by my teacher on viruses, the example he used for lysogenic viruses was a bacteriophage infecting a bacteria. When he was describing how the genetic material was incorporated into ...
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44 views

Why is it possible to make yoghourt with lactose free milk?

The function of the Lactobacillus bulgaricus and Streptococcus thermophilus cultures is to ferment lactose to produce lactic acid. The increase in lactic acid decreases pH and causes the milk to clot,...
2
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1answer
113 views

Commensalism with Virus bacteria [duplicate]

Why people die of infection from microorganism, when germs can keep breeding for longer if they dont kill host? Would not millions of years of evolution make germs evolve to not to kill human but feed ...
2
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1answer
483 views

Role of dam methylase in bacteria

In bacteria, an enzyme called dam methylase (Deoxyadenosine methylase) methylates adenines (A) in the sequence GATC in the new strand formed after replication. What role does this methylation play? ...
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2answers
836 views

Cleavage of RNA by restriction enzymes?

Six restriction enzymes discussed in Sequence-specific cleavage of RNA by Type II restriction enzymes (Murray et al.) have the ability to detect and cut RNA strands with that enzyme's recognition ...
2
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1answer
122 views

Is [O2] different in a 5 mL liquid media?

Is there a drastic difference between top and bottom of the tube, with no stirring?
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111 views

Aside from cockroaches and other insects, which species (of all kinds) are 'immune' to ionizing radiation?

I have just been reading this excellent question and answer about cockroaches ability to withstand ionizing radiation. My question is an extension of that question: aside from insects, which other ...
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1answer
92 views

Loss of antibiotic resistance due to long term storage

How possible is it for antibiotic resistant bacteria stored for a long time to lose such resistance?
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2answers
229 views

Why are aminoglycoside antibiotics particularly effective for aerobic gram negative bacteria?

Aminoglycosides are short chains of sugar molecules with some -OH groups substituted with amine groups. As far as I understand they function by causing bacterial ribosomes to misread RNA and create ...
2
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1answer
646 views

Mass production of bacteria

Is there a way to mass produce a certain species of bacteria? Answers I am looking for should not involve a Petri dish: I am only interested in protocols involving, perhaps, more efficient mediums to ...
2
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1answer
809 views

What bacteria results in a Gram +ve cocci and catalase +ve? What test comes next?

Results so far. We are trying to determine unknown microorganisms in intro to microbiology course. I first did gram stain and they were all cocci morphology, purple color and clumped together (...
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1answer
120 views

Why isn't the DNA in bacteria always split up and replicating?

Isn't helicase always free floating in bacterial cells, and the DNA without a nuclear membrane and uncoiled and freefloating and so why doesn't the helicase keep breaking the double helix of DNA? Also,...
2
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1answer
302 views

How can E. coli affect C. elegans expression?

Plasmids can be transferred to E. coli. These transformed E. coli can be fed to C. elegans to silence its gene expression by RNAi. How can E.coli release RNAi to C. elegans? Even if we assume E. ...
2
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1answer
272 views

Can microorganisms (e.g. bacteria) be damaged or killed by 253,7 nm light?

The question arose in a hospital, where I saw these flyers advertising lamps that emit such light. It was claimed that they can clean the air of microorganisms up to an arbitrary degree. Does anyone ...
2
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1answer
125 views

Do I have to use sucrose to induce a lac promoter?

I'd like to optimize the expression of a Fab fragment in Escherichia coli. For induction of the lac promoter on the pAK400 vector I use IPTG and sucrose. Do I optimize the expression in case I would ...
2
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1answer
670 views

Do bacteria with multiple flagella move faster than bacteria with a single flagella?

Do bacteria with multiple flagella move faster than bacteria with a single flagella? Assuming the flagella are at the same length.
2
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1answer
756 views

Production of processed Insulin in bacteria

Genetically-engineered bacteria are used to produce insulin in industry, but as far as I know, the bacteria can produce only proinsulin. Why is that? What happens in the human body in order to make ...
2
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1answer
149 views

Are added yogurt cultures harmful to the gut?

I have read somewhere that the live culture added to yogurt acting as "healthy" gut bacteria are actually synthetic and harmful. Any truth to this? If so, what are some alternatives to getting good ...
2
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1answer
302 views

Why is it that bacteria and fungi cannot develop resistance against essential oils?

I wonder why bacteria and fungi cannot develop resistance and mutate agains essential oils? For example, some fungi get killed by oregano oil. Being not a biologist, I hope my question is not so ...
2
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1answer
141 views

Quorum Sensing in Vibrio cholerae

This is a figure summarising the quorum sensing mechanism in Vibrio cholerae. In this video by Bonnie Bassler, she explains how quorum sensing can be targeted to control infections. At 15:09 she ...
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1answer
2k views

Mesosome - does it exist?

This wikipedia page says that mesosomes are artifacts produced by chemical fixing techniques. Most of my textbooks still show a mesosome and describes it as having functions like - respiration, ...
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1answer
41 views

Proteins that cannot form biofilm?

I am trying to build a machine learning training set for bacterial protein sequences that form biofilm, and that cannot. I collected the positive sequences from the GO ontology website but for ...
2
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1answer
645 views

Antibiotic resistance of Staphylococcus epidermidis and S. warneri

I am looking for information regarding the antibiotic resistance of S. epidermidis and S. warneri, specifically against penicillin, ampicillin and tetracycline but to no avail. Are there any good ...
2
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1answer
544 views

How does Vibrio cholerae benefit from infecting its host?

As far as I know, V. cholerae secretes a toxin called choleragen into the intestinal lumen which affects the intestinal epithelial cells causing release of Na+ and Cl- ions into the lumen and reducing ...
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1answer
51 views

Parthenogenesis Induction

I have read about Parthenogenesis induction in a paper : "Hagimori T, Abe Y, Date S, Miura K (2006) The first finding of a Rickettsia bacterium associated with parthenogenesis induction among insects. ...