Questions tagged [biochemistry]

The study of chemistry within the scope of biology: the compounds that occur and the reactions involving them in living organisms.

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Do proteins contain phosphorous? If its true then how alfred hershey and martha chase used the statement that proteins do not contain phosphorous?

I hv heard about phosphorus that it is a constituent of certain protein, although we know that no amino acids have phosphorus... and if its true then how Alfred hershey and Martha chase experiment got ...
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Can mold grow on copper pipes?

Couple months ago noticed something was literally, for lack of a better term, eating paint off of lead pipes. And I noticed joints in copper pipe seemed to be "leaking" flux. Now all the water in my ...
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Why are proteins considered Informational macromolecules?

I can understand somewhat why DNA and RNA are considered Informational macromolecules, because they contain genetic information. But why are proteins considered Informational macromolecules?
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Occurrence of non-functional, “spontaneous”, amino acid degradation?

Animals tend to have to consume proteins continuously. Amino acids are often degraded by synthesis of other compounds, including glucose and cellular respiration, catecholamines, etc, accounting for ...
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Can catecholamines degrade back into tyrosine, or, is synthesis irreversible? (in human body)

Catecholamines like dopamine, noradrenaline and adrenaline are broken down with enzymes that catalyze the reaction. Can they degrade back into tyrosine (a conditionally essential amino acid), or is ...
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Any phyla in Animalia that does ammonia assimilation?

A key step in nitrogen metabolism is the initial assimilation of inorganic ammonia into amino acids. Are there phyla within Animalia that do assimilation of ammonium (NH4+)? Phyla with symbionts from ...
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What causes the overactivity of collagen production and expression in the cells of the nail bed/ nail matrix after some traumata?

What is the cellular mechanism of traumatic(sterile) onychodystrophy(hypertrophy)? I.e one hits his toenail and has it removed twice( once after the first trauma and then 3 years after the first ...
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how can we identify the novel protein amino acid sequence and function? [closed]

if there is a novel protein, how would you know the amino acid sequence and how would you determine the number of subunits and the function of that protein? Specifically what molecular techniques we ...
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Values of the blood tests pancreatic amylase and lipase

Anyone know what is considered slightly elevated, moderately elevated and strongly elevated values of pancreatic amylase and lipase? Many different laboratories in my country divide between these ...
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Toxicity of stannous fluoride in toothpaste?

I recently purchased a new brand of toothpaste, and when I looked at the medicinal ingredients, it listed stannous fluoride 0.454% w/w, instead of the regular sodium fluoride. I was a bit concerned ...
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Isopropyl + ethanol as effective disinfectant/biocide

So I have 1 liter of isopropyl alcohol 99.9% and I would like to make it more effective (make it 70% concentration) and at the same time make it last longer as there's a shortage off this stuff. So I ...
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How to recalibrate qpCR reading when incorrect qty of solution is added during DNA elution step?

I have 20 viral DNA samples collected for 6 time points. The DNA content varies over time My DNA extraction protocol step suggests that in the last step of the protocol during elution I had 100uL of ...
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Examples of alkali metal and halogens covalently bonded in biological molecules?

Divalent and monovalent ions are often non-covalently bonded in biological molecules, they are primary building blocks of the machinery of biology. Iodine, the halogon in period 5, is covalently ...
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Does the zeta potential of a nanoparticle generally take into account ligand charge?

For example, if I had a quantum dot nanoparticle with conjugated linker peptides capped with polyarginine tracts. Would the localization of negative charge from arginine change the zeta potential ...
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Why doesn't copper affect human skin?

It's been known for a long time that copper has antimicrobial properties, but if it is so potent, why does it seem to have no effect on human skin or really any large animal?
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Why does Porous-tube Nutrient Delivery System cultivation improve nutritional quality of Okinawa spinach (Gyruna bicolor)?

I wrote a review paper and it involves in growth system of a plant “Okinawa Spinach (Gynura bicolor)”. A research of (Wang, Fu, & Liu, 2015) reported that Porous-tube Nutrient Delivery System (...
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How are Viruses Genetically Modified?

Okay everyone, so I get that you can perform lysis on a bacterium to extract the plasmid, then alter the plasmid using restriction enzymes and ligase, and re insert it back into the bacterium with ...
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Ratio of bleach to water required to disinfect COVID-19?

I am confused about the needed ratio of bleach to water required to disinfect COVID-19 from surfaces. The guide on CDC states that: https://www.cdc.gov/coronavirus/2019-ncov/community/home/cleaning-...
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Relationship between bacteria size and temperature?

I'm, doing a research in a TOC (total organic carbon) degradation in a BAC (biologically active carbon) filter for the grey-water treatment. Recently, I started to wonder if there is a relation ...
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Why is Ibuprofen contraindicated in asthma patients?

So yesterday a patient showed up at the clinic with a massive swelling in his left face region. Upon examination it was found to be due to infected first premolar. Dentist recommended him to get the ...
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how can I determine which dissociable group dissociates first in an amino acid? [closed]

take the amino acid Lysine for example, at a certain pH it has 3 dissociable groups, the alpha carboxyl, the alpha amino, and another amino group in the side chain R. I was assigned 3 pKa numbers for ...
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Does the composition of a meal play a big, or small, role in food's thermogenic effect on the body?

Reading in: "Diet and Health: Implications for Reducing Chronic Disease Risk / Calories: Total Macronutrient Intake, Energy Expenditure, and Net Energy Stores" [NCBI Resources]: "The thermogenic ...
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How are logarithmic trendline equations used to determine antibody concentrations in ELISA?

For example, say you have plotted a standard curve and managed to obtain a logarithimic trendline equation like: y = mln(x) - b And you are working with data that includes: a) standards,their ...
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what's the difference between cofactors and allosteric effectors?

"allosteric regulation (or allosteric control) is the regulation of an enzyme by binding an effector molecule at a site other than the enzyme's active site." does this imply that effector molecules ...
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Is it possible to artificially induce a 40°C fever in a controlled and safe manner? [closed]

In search for any possible new treatments for COVID-19, I am of the understanding that the SARS-nCoV-2 virus may suffer deterioration at high temperatures and high humidity, to perhaps weaken it ...
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Circulating Tumor Cell vs Circulating Tumor DNA

I'm a little confused about the wording of these two phrases and under which context the epithelial-mesenchymal transition occurs. For example: Is it the circulating tumor cell that releases the ...
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How to measure DNA concentration from Acetonitrile Cell Lysis of plant cells?

I'm currently trying to normalise my data to the number of cells present in each sample. Currently, I lyse the cells with cold ACN and centrifuge. The supernatant contains what I'm interested in, and ...
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AChE aging time of organophosphates containing hydroxyl groups

Organophosphorus compounds are known to inhibit the enzyme acetylcholinesterase (AChE). This occurs when the organophosphate phosphorylates the serine-203 residue of the enzyme. If the enzyme is not ...
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How to analyze phosphorylation shift by western blot?

I want to see the phosphorylation shift in my protein of interest. I have created a point mutation in my protein. so that it will not able to go for the phosphorylation compare to my control. i want ...
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How exactly can a drug affecting squalene epoxidase affect the methylation cycle, necessitating the use of 5-MTHF?

I found a case report in which the authors say that Terbinafine might have possibly affected the methylation cycle in a patient, necessitating the prescription of 5-methylfolate to correct the ...
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Rescuing a virus from naked (c)DNA

Reading the articles it seems that for several human virus strains, synthesizing the full-length genome then electroporating into vero cells and incubating a few days gave birth to living viruses that ...
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Tryptophan structure

Currently trying to memorize the different amino acid structures for my own learning purposes. I think I am confusing myself, and would like clarification to see if it matters where the NH and double ...
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What happens when you cook tree spinach with aluminum?

The Internet is filled with warnings that you shouldn't cook tree spinach(Cnidoscolus aconitifolius) in aluminum, because it will react and create toxins that cause explosive diarrhea when consumed. ...
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How can one relate in vitro studies of caffeine (dose expressed as concentration) to dietary intake of caffeine (dose in mass)?

Having difficulty making the data in the below charts to apply to daily life. What is the right interpretation and implementation? How are the “in vitro” results of the below charts relevant to levels ...
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Bimodal frequency distribution of size of protein loops

The graph of number of amino acid (AA) residues in a loop Vs the frequency of their occurrence in proteins largely follows a tending-to-zero pattern. However, there appear to be some specific number ...
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In vivo, does it take energy to move double bonds from cis to trans in long chain unsaturated fatty acids

In vivo, I understood that because all polyunsaturated fatty acids bonds are -cis they are crowded, kinking the molecule, and because of that, the oxidative enzymes cannot initially access them. They ...
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About the mechanism of coupled reaction/metabolism with ATP

I am not in the field of biochemistry so this may be a rookie question or misconception. I heard occasionally about the energy "released" from ATP hydrolysis fueling (endergonic) biological reactions....
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What makes iodine an effective antiseptic?

I'm thinking about tincture of iodine, potassium iodide (Lugol's), and povidone-iodine (PVP-I) specifically, which, as is my understanding, work by solubilizing elemental iodine in an aqueous solution....
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What's unique for vitamin B-12 group?

I've been trying to figure out what makes the vitamin B group really big with 8 vitamins. Chemically they have different structures. Solubility: not only vitamins from this group are water soluble ...
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pH on skin penetration of salicylic acid

I have found that at lower pH, there is more free salicylic acid (due to pKa of roughly 3), which is neutral/uncharged and then can penetrate the skin's oils which are non-polar. At higher pH, more ...
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How to derive Hill equation (one specific part)

There is just one specific step in the derivation of the Hill equation for haemoglobin which I can't understand. Step from: $Y = \frac{(p\ce{O2})^n}{K_d + (p\ce{O2})^n}$ To: $Y = \frac{(p\ce{O2})^n}{(...
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Can microbes become “overweight”?

Just out of some weird thoughts, can a microbe (any single-celled organisms, accepting answers for both prokaryotes and eukaryotes) ingest too much food (e.g. via absorbing too much involuntarily ...
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Can bacteria pick up lethal plasmids?

I am sorry if this question is too general, and does not have any concrete answer. I was explaining to my non-biology-background friend about plasmids and how they are picked up by bacteria from the ...
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Which type of test tube should not be used for blood collection?

The following question is presented in my biology textbook: You are required to draw blood from patient and keep it in a test tube for analysis of blood corpuscles and plasma. You are provided with ...
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How does salicylic acid (2-hydroxybenzoic acid) break down desmosomal proteins to exfoliate skin?

I've been searching for an explanation to salicylic acid's property of being able to break down desmosomal proteins, such as desmogleins in order to disrupt the outer layer of skin. Can someone ...
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A question about L-Citrulline

I know that L-Citrulline enters in the second step of urea cycle in the liver mitochondria and I wonder if a person takes an overdose of L-Citrulline wouldn't that cause mild hyperammonemia or at ...
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What are the disadvantages of myelin

The myelination of axons has plenty of advantages. It increases signal speed in axons, and thereby reduces reaction times. This is, of course, very good for the survival of the animal in question. ...
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How much heme is in cooked pork and beef; why is cooked pork (“the other white meat”) not red?

The new video See how Impossible Pork will make you forget about pig meat includes a very short discussion of the addition of heme to the product to make it taste like beef the deep red color of a ...
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Why does absorption (at 260nm) of ssDNA increase with temperature?

In the graph below, I understand why A260 of double stranded DNA would increase with temperature increase, as more of the DNA becomes single stranded (and A260 of ssDNA is higher than that of dsDNA ...
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Regulation of the TCA cycle and glycolysis by adenine nucleotides

Why is the tricarboxylic acid cycle regulated by the ADP/ATP ratio as stated in the following quote : Isocitrate dehydrogenase is allosterically stimulated by ADP, which enhances the enzyme's ...

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