Questions tagged [biochemistry]

The study of chemistry within the scope of biology: the compounds that occur and the reactions involving them in living organisms.

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Rice left in water for several months - what biological reactions or growths would occur?

In an attempt to make a hair care product, uncooked white basmati rice was left in an air-tight container half filled with tap water for several months. When opening the container, the smell was ...
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3answers
173 views

Using nanodrop for analysing biological samples other than nucleotides

I am a 3rd timer postdoctoral fellow with some experience in molecular biology and biochemistry, but major skills in Zoology and Natural History. I am studying some natural extracts, and isolating ...
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0answers
11 views

How to troubleshoot in vitro formaldehyde fixation for nucleosomes?

For an experiment, I am trying to fix the mononucleosomes (100ng) using formaldehyde as crosslinking agent in HEPES buffer. I have been using 2% formaldehyde in a reaction buffer containing 1mM EDTA, ...
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2answers
97 views

Edible and non-edible leaves [closed]

For humans, what's the difference between an edible leaf like lettuce, and a non-edible (in the sense of null nutritional value) leaf like grass?
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1answer
33 views

Cellular respiration in carnivorous animals

What is the equation for cellular respiration in carnivores as they don’t consume carbohydrates to break down into glucose in the following manner: Glucose + oxygen -> water + CO2 +energy. Do they (...
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2answers
8k views

What is the mechanism behind “acquired” alcohol tolerance?

I can understand natural variation in alcohol dehydrogenase (ADH) in a population leading to variation in rate of inebriation (after controlling for other variables -- e.g., mass, food consumption, ...
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2answers
473 views

Potato Power. Self sustaining medium using a living potato plant possible?

The second picture are potatoes wired in parallel. I understand that the potato is the medium for a chemical reaction between the copper and zinc. That aside, would wire and in soil potato plant ...
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1answer
79 views

What is the standard method for measuring soil and substrate acidity

Soils and other plant substrates differ a lot in their moisture content. In dry matter a pH is not measurable without adding water, but this has the problem of the resulting value not being ...
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25 views

What impact does substrate bonding have on GMO’s if one at all

Do GMOs increase the effect of substrate bonding? Does it minimize activation energy? Does it change concentrations or bonding potentials?
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10answers
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Why are there no organisms with metal body parts, like weapons, bones, and armour? (Or are there?)

Reading this question, Why are there no wheeled animals?, I wondered why no organisms seem to make use of the tensile and other strengths of metal, as we do in metal tools and constructions. I am ...
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2answers
48 views

Can something cause both breaks and cross-links in DNA?

A double strand break in DNA is exactly that: the strands of DNA are severed. A cross-linkage occurs when something forms a covalent bond between two nucleotides in DNA. However, is it possible for ...
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0answers
113 views

Living potato clock? [duplicate]

Could a potato stay alive and power a clock while growing in the ground? I know how a potato clock works as a electrochemical cell and involves chemistry, but I am only interested in a growing ...
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1answer
8k views

How does a Plasmodium move?

Plasmodium sp. does not have any locomotory organs. So, how does it move? What biochemical process allows it to move?
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10k views

Why does the ring finger not move independently?

Why is it that we need to move little finger in order to move ring finger with it? For example, put your palm on a table and fold your middle finger inside. You won't be able to move your ring finger ...
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0answers
17 views

Would Cu1 or Cu2 oral supplementation be most likely to aid lysyl oxidase (LOX) production?

Forgive the basic question, I'm not a biochemist but am interested in understanding the below: LOX of course is key for interlinking collagen & elastin. At the same time, it is claimed that Cu1 ...
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1answer
3k views

Is there any advantage of having mitochondria for aerobic respiration?

If we consider the pathway of breakdown of glucose which includes glycolysis, the citric acid cycle and the electron transport chain, all these processes takes place in some prokaryotes and ...
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2answers
69 views

Do histones constitute the largest proportion of the protein in chromosomes at mitosis?

Do histones contribute more (by mass) than non-histone proteins in the chromosomes formed during mitosis?
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2answers
180 views

How do organophosphates actually work?

The common explanation as to what the primary mechanism of action for organophosphates (and carbamates) is is the inhibition of the enzyme acetylcholinesterase and resulting buildup of acetylcholine ...
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1answer
29 views

Difference between cerebroside and globoside

I have a general idea about their difference that cerebrosides have a single sugar while globosides have more than one sugars. This is the structure of a ceramide (syphingosine and a fatty acid ...
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0answers
50 views

Why is double stranded DNA considered to be one molecule? [duplicate]

Wikipedia, for example, refers to DNA as "a" molecule, not two separate molecules forming a double-helix structure. Isn't this technically incorrect? There are two separate polynucleotide strands (...
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1answer
70 views

Blood acidity and bones

By consuming acidic foods, could one cause their blood to become acidic and therefore cause an acid+base reaction between their blood and bone? I ask this because i recently discovered bone is a ...
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0answers
5 views

When should antibody/antigen be pluralised? [migrated]

I am doing my thesis corrections, and my examiner (an engineer) has different ideas about whether the word should be pluralised than those I am used to, as I am a non-biochemist, I wanted advice on ...
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0answers
30 views

Cooperative Enzyme Question

I'm currently studying for the MCATs. After doing some biochemistry practice questions, I came upon a question that I believe may be incorrectly posed. I was wondering if I'm correct in saying this or ...
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4answers
722 views

Is there a known glucosepane cross-link breaker?

I read the following on wikipedia: There is, however, no agent known that can break down the most common AGE, glucosepane, which appears 10 to 1,000 times more common in human tissue than any ...
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1answer
526 views

Meaning of arrows in 3D representation of proteins

Below is a three-dimensional representation of the protein, CD4. I would like to know what the arrows and the thin wires between them mean.
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3answers
11k views

Why is glycine considered a nonpolar amino acid but a polar molecule?

Glycine has a dipole moment, so why is it considered a nonpolar amino acid when discussing its occurrence in proteins? Also, is the backbone of a protein nonpolar?
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1answer
88 views

Does eating nonsalty food with MSG make you thirsty?

Assume that you're thirsty after eating food that didn't taste salty while you were eating it. Quora posts and https://msgdish.com/msg-problems-and-answers/ still impute the thirst to salt: The ...
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1answer
34 views

Why is beta- D fructofuranose two different structures when in free form and as part of sucrose?

This is the structure of fructose in free form: The right moiety is the fructose as part of sucrose. The left one being glucose: Both of the fructose are beta-D fructofuranose. But as it can be ...
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20 views

Can a lentivirus expressed protein that is undetectable by western blot be detected by BioID?

Cloned a single herpesviral gene into pCDH-EF!-GFP and see phenotypic effect on cells of interest and viral gene transcripts BUT unable to detect ANY protein using HRP boosted western blot. Even with ...
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47 views

Does cat urine really fluoresce? If so, why?

I temporarily got caught in the Sad and Useless website, and found Science is Fun. Most of the claims seem plausible, but I can't guess why cat urine would fluoresce. Comments have directed me to ...
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56 views

Cracking sounds made by glowing plankton

Recently I had opportunity to swim in place with glowing plankton, it was amazing experience and it as well gave birth to few questions in my head. I manged to google that they can glow thanks to ...
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0answers
58 views

Could an uncut umbilical cord benifit a baby?

How long and would there be any benefit like adding an IV line to the uncut umbilical cord after a baby is born? Can keeping the umbilical cord attached (and for how long) be beneficial or harmful ...
2
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1answer
44 views

How would a medication cause a one to maintain a different weight?

This isn’t really a medical question, I’m just really curious about this. I was maintaining weight A and then started taking a medication that brought me to weight B. While on the medication, I ...
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27 views

SDS-PAGE compared to western blotting

SDS-PAGE and western blotting are both used to separate and isolate proteins. My question is, which technique should be used when? Does one of them has advantages over the other one?
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3answers
530 views

Getting PCR amplification at annealing higher than Tm!

I am amplifying a gene where in a gradient pcr i am getting amplification at an annealing temperature about 5 degrees (67) higher than Tm (62.5)? What is wrong here? Also, I am getting a very strong ...
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55 views

What is the difference among biochemistry, molecular biology, molecular genetics and structural biology?

I have never heard of straigthforward definitions of these fields in my college lectures, and the Internet searches were not very helpful. However, from what I have learned at different subjects, this ...
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2answers
160 views

What structural features make a molecule a potent opioid receptor agonist?

For instance, take morphine. It is used as a baseline for measuring the potency of opioid agonists. Its structure looks like this: But then, take heroin, around three times as potent, its structure ...
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1answer
64 views

Why magnesium ion is needed for activity of G-proteins?

I was studying about G-protein coupled signaling sysytem, somewhere they have mentioned about need of Mg$^{2+}$ for acticty of G-protein and related it to increase of cyclic AMP production. On further ...
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1answer
51 views

Immunoprecipitation compared to western blotting

Immunoprecipitation and western blotting are both used to locate a specific protein within a sample and to isolate it. In immunoprecipitation, a specific antibody and agarose beads ( or other ...
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1answer
38 views

Design rules for DNA linkers

I want to use double stranded DNA linkers to physically bind two "things" together, by grafting ssDNA on each one of them and using DNA hybridization as the locking mechanism. I do not expect the ...
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34 views

What substances can selectively destroy certain cells?

Recently, I've watched a documentary about how, in the 1980s, people were buying and using drugs from the streets and then becoming paralyzed a few days afterwards. The drugs that they were using were ...
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1answer
2k views

Purpose of the conversion of 3-PGA to G3P in the calvin cycle?

3-PGA ($\ce{C_6H_7O_7P}$) is converted to G3P ($\ce{C_6H_7O_6P}$) before it is able to be made into glucose, with an expansion of 6 ATP and 6 NADPH. My question is why this process is needed and why ...
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0answers
207 views

Do cats produce DMT?

Edit: If somebody (anybody) wants to add an answer so the bounty won't go to waste, please do so! The comments have already introduced me to the Kegg metabolism database, some interesting papers, and ...
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2answers
189 views

Is the chemical composition of urine detrimental or beneficial for a tree?

Seeing as a lot of people around the world urinate against trees it came to mind that I never thought about how the tree responds to this. Is it detrimental for a tree if people urinate against them? ...
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2answers
30 views

Preparing sample for SDS PAGE

I have more than 10 cell lysate samples (70 µL each) whose concentration varies from 1.9 mg/mL to 4.8 mg/mL. I have 5X and 2X SDS sample buffers. I would like to prepare SDS PAGE samples in such a way ...
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1k views

Can cysteine alone change pigmentation?

According to this graph (from here): cysteine contributes to pheomelanogenesis, and having a high enough concentration of cysteine makes the shift towards pheomelanin instead of eumelanin. So my ...
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2answers
2k views

Is NMDA produced in the body?

On the wiki page for NMDA it says that NMDA is a synthetic substance that mimics glutamate. So why does the body not use glutamate instead of NMDA? Also how is it possible that our body can produce ...
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1answer
1k views

Are the bacteria in the stomachs of unborn babies beneficial?

Babies are born with bacteria in their stomachs. I heard on The radio that when a child is given antibiotics for the first time unique bacteria in the stomach are destroyed and cannot be replaced. Is ...
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Does a generator potential pass along a nerve the same way an action potential does?

I have read that a generator potential is a localized depolarization of a membrane. Does that mean that it does not pass along a neuron the same way an action potential does ? If not, then how do ...