Questions tagged [biochemistry]

The study of chemistry within the scope of biology: the compounds that occur and the reactions involving them in living organisms.

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9 views

Does the brain absorb heme and non-heme iron differently?

I know that for the brain to absorb iron, the iron must first pass through the blood brain barrier. Is this absorption different for heme and nonheme iron?
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How many histidine tags are in the glucose dehydrogenase?

How many histidine tags are in the glucose dehydrogenase (from Bacillus subtilis) and how does this number influence the process of affinity chromatography?
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Basic Molecule Editor for use in journals / theses?

My background is Geosciences and Physics and I am going to study water and biomolecules in the Autumn. I was reading a PhD thesis about peptides and peptoids by someone in my research group and I had ...
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13 views

Can receptors exist on nanoparticles?

Given I have x amount of Gold nanoparticles or some type of nanoparticles that I wanted to bond together to create some sort of molecule or even a macro structure. Would it be possible to mimic the ...
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Overcoming palmitic acid intolerance

What can stop the body from being able to process or break down palmitic acid? The acid stays in the stomach and burns causing a similar reaction as heart burn when the volume is high. Heart burn ...
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How do microtubules “Push” agaisnt the cell membrane if they are polymerizing at the very tip that makes contact with the membrane?

Trying to understand the reasoning behind the various Push VS pull microtubule arrangements, if it is "push" then I dont see how it would be able to push up against the cell membrane while ...
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Are there any organisms that do not perform glycolysis (or anything similar)?

To clarify I know that there are alternative pathways similar to glycolysis, what I mean is are there any organisms that perform a different pathway that is radically different from glycolysis, but ...
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35 views

Why do all anabolic reactions need energy?

Catabolism is the process of breaking down larger molecules into smaller ones and this 'always' releases energy. Anabolism is the process of building up larger molecules, effectively a polymerisation ...
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36 views

Why are proteins considered Informational macromolecules?

I can understand somewhat why DNA and RNA are considered Informational macromolecules, because they contain genetic information. But why are proteins considered Informational macromolecules?
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17 views

Any phyla in Animalia that does ammonia assimilation?

A key step in nitrogen metabolism is the initial assimilation of inorganic ammonia into amino acids. Are there phyla within Animalia that do assimilation of ammonium (NH4+)? Phyla with symbionts from ...
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9 views

How to recalibrate qpCR reading when incorrect qty of solution is added during DNA elution step?

I have 20 viral DNA samples collected for 6 time points. The DNA content varies over time My DNA extraction protocol step suggests that in the last step of the protocol during elution I had 100uL of ...
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22 views

Does the zeta potential of a nanoparticle generally take into account ligand charge?

For example, if I had a quantum dot nanoparticle with conjugated linker peptides capped with polyarginine tracts. Would the localization of negative charge from arginine change the zeta potential ...
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37 views

Relationship between bacteria size and temperature?

I'm, doing a research in a TOC (total organic carbon) degradation in a BAC (biologically active carbon) filter for the grey-water treatment. Recently, I started to wonder if there is a relation ...
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53 views

Why is Ibuprofen contraindicated in asthma patients?

So yesterday a patient showed up at the clinic with a massive swelling in his left face region. Upon examination it was found to be due to infected first premolar. Dentist recommended him to get the ...
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26 views

How are logarithmic trendline equations used to determine antibody concentrations in ELISA?

For example, say you have plotted a standard curve and managed to obtain a logarithimic trendline equation like: y = mln(x) - b And you are working with data that includes: a) standards,their ...
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20 views

How to measure DNA concentration from Acetonitrile Cell Lysis of plant cells?

I'm currently trying to normalise my data to the number of cells present in each sample. Currently, I lyse the cells with cold ACN and centrifuge. The supernatant contains what I'm interested in, and ...
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36 views

AChE aging time of organophosphates containing hydroxyl groups

Organophosphorus compounds are known to inhibit the enzyme acetylcholinesterase (AChE). This occurs when the organophosphate phosphorylates the serine-203 residue of the enzyme. If the enzyme is not ...
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64 views

Can microbes become “overweight”?

Just out of some weird thoughts, can a microbe (any single-celled organisms, accepting answers for both prokaryotes and eukaryotes) ingest too much food (e.g. via absorbing too much involuntarily ...
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41 views

Where do methyl groups in the human body come from?

Where do methyl groups in the human body or other mammals come from? Do we synthesize them (where?) Do we get them from our diets (in what? anything beside methionine?) Do our gut microbes produce ...
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21 views

How does lipoid pneumonia lead to acute respiratory distress syndrome (ARDS)?

How does lipoid pneumonia lead to acute respiratory distress syndrome (ARDS)? The vaping illnesses that have been happening on the news in the United States are being caused by the federal ...
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27 views

Do pyrocalciferol and isopyrocalciferol form in vertebrate skin?

I heard that pyrocalciferol and isopyrocalciferol can only be formed above 100°C/212°F, yet some images in the web depict them as forming in vertebrate skin. So, I wonder if pyrocalciferol and ...
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What's the difference between mannanase and mannosidase?

Specifically, in Aspergillus spp mannandegrading enzymes. I saw this table at the BRENDA's page for 3.2.1.25 and 3.2.1.78 enzymes and the synonims are reciprocal to me. So, why a mannanase is also (...
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17 views

Does food grade diatomaceous earth contain mercury

Not sure if this is the right site to ask this question, but since diatomes are or once were biological creatures it seemed to fit this one more than any of the others. I have been supplementing my ...
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Would DMSO Be An Effective Solvent For Creating A Transdermal Absorption of NMN + Pterostilbene + TMG In Humans?

I've read conflicting information on this. If not DMSO, is there a better solvent to use?
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Why aren't diatoms' shells used as a temperature proxy, but foraminifera shells are?

Changes in oceanic oxygen isotope ratios (O-18 to O-16) are reflected in the shells of ancient foraminifera. But I never hear of diatom shells being used, even though they were/are at least as ...
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20 views

Coagulation of protiens of different products

Protein of egg coagulates on 60 degree but proteins of milk do not coagulate at high temperatures..why? What is the main reason behind all this?
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29 views

Theoretical basis for amphetamine salt combo(Adderall) vs Dextroamphetamine

After re-reading the help questions I think this belongs here as I am more interested in the biochemical motivations rather than any medical query or advice(but may also fit Chemistry SE). So from ...
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55 views

Ligand-binding assays: IC50, EC50 and Kd

I am reviewing several MHC-peptide binding affinity predictors trained on IEDB data. Quantitative records for MHC class I allotypes come from a lot of different assays and, by extension, have ...
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25 views

How are humanized antibodies made?

What kind of antigen is used to provoke/induce an immune response if you are trying to make therapeutic humanized antibodies for cancer and alzheimer's disease? For example, if you wanted to make an ...
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52 views

What enzyme or bacteria could dissolve plastic?

What enzyme or bacteria could dissolve plastic aside from acetone? Preferably, low-cost and environmentally friendly. Any suggestions would be helpful. I'm targeting for thin plastic (low-density ...
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29 views

Biochemistry of pleasure of/desire for physical exercise in mammalians and humans?

Are there some known biochemical mechanisms, that induces desire for/pleasure of physical exercise in mammalians and humans? I have heard, that horses can not live without physical exercise and they ...
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30 views

How to troubleshoot in vitro formaldehyde fixation for nucleosomes?

For an experiment, I am trying to fix the mononucleosomes (100ng) using formaldehyde as crosslinking agent in HEPES buffer. I have been using 2% formaldehyde in a reaction buffer containing 1mM EDTA, ...
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123 views

Does cat urine really fluoresce? If so, why?

I temporarily got caught in the Sad and Useless website, and found Science is Fun. Most of the claims seem plausible, but I can't guess why cat urine would fluoresce. Comments have directed me to ...
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63 views

What is the difference among biochemistry, molecular biology, molecular genetics and structural biology?

I have never heard of straigthforward definitions of these fields in my college lectures, and the Internet searches were not very helpful. However, from what I have learned at different subjects, this ...
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89 views

The effect of salt, pH and temperature to the salivary amylase

If I prepare the following set of tests, incubate for 15 mins, then add 2-3 drops of iodine solution to each test tube: Salt test (1ml starch solution + 0.5 ml enzyme + 1ml 1M NaCl, incubate at 37 oC ...
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94 views

Does our brain use up more ATP after smoking cannabis?

kind of an amateur here. If the firing of the neurons' signals uses up some ATP, and smoking cannabis makes them fire off more quickly, do our brains use up more ATP to sustain that rapid firing?
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17 views

What is the composition of a standard diluent buffer in a leptin ELISA kit?

I am trying to find the composition of the diluent buffer used for the dilution of a human leptin stock to be used in an ELISA assay. The manufacturer of the kit (https://www.thermofisher.com/elisa/...
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16 views

Coacervater were more like Viruses or Cells?

The answer considers them as first formed living cells as they were precursor of life. But they had no lipid bilayer nor any cellular organization, shouldnt they be more like Viruses ,or like Prions ...
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114 views

How does the body differentiate between sources of good and bad cholesterol?

I keep hearing "good cholesterol, bad cholesterol" everywhere, and how certain food sources of cholesterol raise LDL and certain other raise HDL. I don't understand how any food can increase ...
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49 views

Why is plasma glucose concentration not double that of whole blood?

It is known that the concentration of plasma glucose is 12% higher than that of whole blood. But since 45-50% of whole blood is red blood cells, shouldn't the plasma glucose be almost double — since ...
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36 views

What is the difference between GFP and rhodopsin?

GFP and Rhodopsin are both fluorescent proteins. What is the main difference between GFP and Rhodopsin in terms of how they work? I know that their excitation and emission wavelengths are different, ...
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73 views

What is the most readily usable form of nutrients/energy source for heterotrophic microorganism in general?

Consider a nutritious mixture containing abundant soluble sugars, soluble lipids and soluble protein etc. Would heterotrophic microbes preferentially use sugars first > lipids > proteins > insoluble ...
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121 views

MOPS Media Recipe Protocol - Well Defined

I am trying to find a clear protocol for making MOPS media. However, when looking online, I am finding that this media is loosely defined and I can not find a good protocol. (I have been looking for a ...
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52 views

What biproducts do vinegar eels produce?

What chemicals, acids, gases or other biproducts do vinegar eels (Turbatrix aceti) produce (in raw vinegar and/or in the vinegar-creation process, which process may have them in environments including ...
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210 views

Is dietary fat directly stored in adipose tissue or is it immediately metabolized?

I know that fat goes to adipose tissue and even sugar gets converted to fat and goes to adipose tissue, but what happens to fat (unsalted cow milk butter) I just ate? Will it give me energy now, or ...
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164 views

Diazepam with Aluminum Hydroxide

Some common medicinal preparations contain diazepam with aluminium Hydroxide, there may be other compounds [e.g. magnesium trisilicate and polydimethylsiloxane with Aluminum Hydroxide and Diazepam as ...
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peritoneal dialysis Vs Hemodialysis?

Their are basically two types of dialysis: 1.peritoneal dialysis 2.hemodialysis, My Question is Why the peritoneal dialysis needs to be carried out on everyday BUT hemodialysis is carried out on only ...
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Amount of Na+ needed to be transfered in order to depolarize the membrane?

I found out that the number of $Na^+$ ions that is needed to be transfered across the membrane to make it depolarized is a small number. In what way its proved? (Goldman equation maybe?)
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111 views

Does the source of caffeine affect the biological impact?

I've recently decided to give up my morning coffee in lieu of a caffeine pill. Both are around 200mg (my Tim Horton's XL 4x4 was 240 mg of caffeine) of caffeine. We were talking about the various ...
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107 views

Determination of Ageing by ECG inclusions/exclusions?

I am studying ageing and considering ECG signal because of its high sensitivity in theory (escardio). Some factors Sensitivity Gender Medical treatment ... Benchmark: RTG dental + wrist uncertainty ...