Questions tagged [biochemistry]

The study of chemistry within the scope of biology: the compounds that occur and the reactions involving them in living organisms.

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Is there a software simulator for monomeric formation, a la Miller-Urey?

At the moment, does any widely-available software exist for small-scale, programmable simulation of monomeric formation in specific conditions? Could one, for instance, recreate the Miller-Urey ...
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70 views

What happens to Bacterial Cells stored at 4 degrees?

4C is when water is most dense and is not so low as to cause ice crystal formation. For short term storage bacterial cultures are often simply kept in a fridge at 4C. I guess that this significantly ...
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plant uptake of large molecules

I have read several studies concluding that plants can indeed take up molecules with a molecular weight larger than 390 g/mol. Does this mean plants do also take up large molecules like hormones if ...
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10 views

Why does hydroxylation of fatty acids occur in the middle of the acyl chain and increase fluidity?

I wanted to check and see why a) hydroxylation of fatty acids is most likely to occur in the middle of the acyl chain and b) why that increases fluidity. If our goal is to increase fluidity, then by ...
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8 views

Lipids triacylglicerol

I wanted to ask about triacylglycerol fatty acid, as you know its got 3 fatty tails. A hydrogen-saturated tail creates more van der Waals connections with neighboring fatty acids and the melting ...
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17 views

Muscular tetany and hypocalacemia

Decreased serum calcium level leads to increased excitability of neuron and at same time decreases the contractibilityof the muscle fibres.But still its causing tetany.Wouldn't these two counteract to ...
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14 views

What is the composition of a standard diluent buffer in a leptin ELISA kit?

I am trying to find the composition of the diluent buffer used for the dilution of a human leptin stock to be used in an ELISA assay. The manufacturer of the kit (https://www.thermofisher.com/elisa/...
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43 views

Are potential calories/ATP lost from fat when converted to ketones?

When insulin levels are low, the liver begins to oxidize fat. The liver oxidizes fat by an incomplete process that yields ketone bodies. The incomplete oxidation of fatty acids by the liver yields ...
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16 views

Coacervater were more like Viruses or Cells?

The answer considers them as first formed living cells as they were precursor of life. But they had no lipid bilayer nor any cellular organization, shouldnt they be more like Viruses ,or like Prions ...
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109 views

How does the body differentiate between sources of good and bad cholesterol?

I keep hearing "good cholesterol, bad cholesterol" everywhere, and how certain food sources of cholesterol raise LDL and certain other raise HDL. I don't understand how any food can increase ...
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46 views

Why is plasma glucose concentration not double that of whole blood?

It is known that the concentration of plasma glucose is 12% higher than that of whole blood. But since 45-50% of whole blood is red blood cells, shouldn't the plasma glucose be almost double — since ...
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33 views

What is the difference between GFP and rhodopsin?

GFP and Rhodopsin are both fluorescent proteins. What is the main difference between GFP and Rhodopsin in terms of how they work? I know that their excitation and emission wavelengths are different, ...
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69 views

What is the most readily usable form of nutrients/energy source for heterotrophic microorganism in general?

Consider a nutritious mixture containing abundant soluble sugars, soluble lipids and soluble protein etc. Would heterotrophic microbes preferentially use sugars first > lipids > proteins > insoluble ...
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109 views

MOPS Media Recipe Protocol - Well Defined

I am trying to find a clear protocol for making MOPS media. However, when looking online, I am finding that this media is loosely defined and I can not find a good protocol. (I have been looking for a ...
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44 views

What biproducts do vinegar eels produce?

What chemicals, acids, gases or other biproducts do vinegar eels (Turbatrix aceti) produce (in raw vinegar and/or in the vinegar-creation process, which process may have them in environments including ...
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198 views

Is dietary fat directly stored in adipose tissue or is it immediately metabolized?

I know that fat goes to adipose tissue and even sugar gets converted to fat and goes to adipose tissue, but what happens to fat (unsalted cow milk butter) I just ate? Will it give me energy now, or ...
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145 views

Diazepam with Aluminum Hydroxide

Some common medicinal preparations contain diazepam with aluminium Hydroxide, there may be other compounds [e.g. magnesium trisilicate and polydimethylsiloxane with Aluminum Hydroxide and Diazepam as ...
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98 views

peritoneal dialysis Vs Hemodialysis?

Their are basically two types of dialysis: 1.peritoneal dialysis 2.hemodialysis, My Question is Why the peritoneal dialysis needs to be carried out on everyday BUT hemodialysis is carried out on only ...
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32 views

Amount of Na+ needed to be transfered in order to depolarize the membrane?

I found out that the number of $Na^+$ ions that is needed to be transfered across the membrane to make it depolarized is a small number. In what way its proved? (Goldman equation maybe?)
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111 views

Does the source of caffeine affect the biological impact?

I've recently decided to give up my morning coffee in lieu of a caffeine pill. Both are around 200mg (my Tim Horton's XL 4x4 was 240 mg of caffeine) of caffeine. We were talking about the various ...
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106 views

Determination of Ageing by ECG inclusions/exclusions?

I am studying ageing and considering ECG signal because of its high sensitivity in theory (escardio). Some factors Sensitivity Gender Medical treatment ... Benchmark: RTG dental + wrist uncertainty ...
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45 views

Avoid Blood lyse color remain in DNA extraction

I tryed several times to extract DNA from freezed blood sample whit Qiagene kit but it gave very small amount of product due to good purity then I try extract manually by using CTAB PVP method and ...
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124 views

Root etiology of non-pituitary low T4 and low TSH

Please note: I'd like to preface this question by stating that this is neither homework nor me seeking medical advice. I am simply trying to understand the biological, physiological root etiology ...
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49 views

Consumption of different type of energy when one is inactive

I'm wonder if there is any study done on this and what are the results? how much of each type of energy one's body(healthy normal person) uses when inactive(seating down) I know there are alot of ...
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24 views

Size of the glycan chain in a glycosylated protein

I would like to know where I can find the (experimental/estimated) distribution of glycan sizes attached to proteins.
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507 views

What is the probe that absorbs at 450nm in the presence of NADH in this assay?

The colorimetric assays by Biovision and Sigma Aldrich seem to utilise a probe that binds to or reacts with NADH in order to cause absorbance at 450nm which can then be quantified by a ...
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45 views

can magnesium bicarbonate be absorbed in the mouth?

Can Magnesium Bicarbonate be absorbed in the mouth?" Magnesium Bicarbonate occurs naturally in some mineral waters.
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67 views

Is α-keratin a fully functional protein?

Is α-keratin before it coils with another polypeptide, makes chains, and build intermediate filaments a fully functional protein? I mean, is the single monomer of α-keratin a protein or does it have ...
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34 views

what happens when you put enhancer and caat box in prokaryotes

what happens when you put enhancer and caat box in prokaryotes
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440 views

dNTP concentration for reverse transcription from RNA to cDNA

Does it affect the results if I use the half of dNTP amount in a reaction that it says in my protocol? I usually use 4 microlitres per reaction and this time I did a mistake and I used 2. The final ...
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53 views

Is it possible to stop more/extra fat from being stored in the body?

I don't mean simply by restricting eating or upping the activities, but a way to basically stop the body from adding more fat on itself down on the bio level. For example, I'm not too knowledgeable on ...
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54 views

Difference between cerebroside and globoside

I have a general idea about their difference that cerebrosides have a single sugar while globosides have more than one sugars. This is the structure of a ceramide (syphingosine and a fatty acid ...
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1answer
17 views

Protein Pathways, Superfamilie, Function database/website?

I'm going trough some old data - but I'm running into this problem that all website we used use is not existing anymore. What I have is a very long list of proteins with accession number (refSeq from ...
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180 views

Can streptavidin be conjugated with EDC-activated carboxylates?

Recently I conducted an experiment to conjugate amine-modified oligonucleotides with hydrogel particles having carboxyl groups. However, the fluorescence (from hybridized FAM-DNA) only appears on a ...
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354 views

What are internal and external osmotic pressures when referring to a cell?

I've seen the term used but cannot find a clear answer: What are internal and external osmotic pressures when referring to a cell?
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62 views

Can an RNA anneal to its complementary (not reverse) sequence?

Say I have a target RNA strand: 5'--AAUGGCCCUUUAAA-->3' I know that this strand will readily anneal to the sequence 3'<--UUACCGGGAAAUUU--5' which is the reverse-complement. I'm wondering if ...
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102 views

Why is the macromolecular composition of a human cell seemingly hard to find?

I am trying to find a 3D rendering or list of molecules by volume in a cell (originally I was searching for human, but I'll take any at this point!). I understand the open-endedness of the question: ...
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1k views

Types of heparin

My textbook(biochemistry 4th edition, U. Satyanarayana) says that the following structure is of heparin and it's name should be D-Glucuronate 2-sulfate, N-sulfoglucosamine 6-sulfate. I searched on ...