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Questions tagged [biochemistry]

The study of chemistry within the scope of biology: the compounds that occur and the reactions involving them in living organisms.

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What is the half-life of dNTPs at 95⁰C?

I'm looking for the half-life of dNTPs, either as a whole or broken into individual bases, at 95 degrees C (or similar). A titration would be great if that exists. I can provide more specifics if need ...
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75 views

Cold water sour taste

Recently I underwent a wisdom tooth extraction, and while there is still numbness I have observed something peculiar. If I drink cold water, it tastes sour, however the same effect is not observed ...
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2k views

What happens at the top of the energy/enzyme graph?

At the very peak, the energy is in a state of activation energy. Here, is the substrate just attaching to the enzyme, or is is substrate already breaking?
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49 views

Does Doxylamine have any Influence on the Monoaminergic System?

Does doxylamine have any affects on the monoaminergic system? Specifically I am interested in adrenergic or serotonergic activity but additional information on its dopaminergic activity won't hurt ...
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320 views

Virucides - Herbal and otherwise, for HHV6 type virus or any inner-cell virus

Does anyone know where I can find a reliable list of virucides (not anti-virus) that can be used by humans? This virucide must be able to affect viruses that are already inside the cells. St John's ...
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24 views

Can an epileptic seizure start in the spinal cord?

My understanding of an epileptic seizure is caused by "invalid electrical signaling" in the brain. Can these start from the spinal cord (outside the brain) and move into the brain and cause an ...
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32 views

Light/Heavy Isotopes in Living Organisms

I was wondering, why are lighter isotopes (e.g. carbon-12 as opposed to carbon-13) preferentially used by life on Earth? Is their increased stability the only factor? Thanks in advance!
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47 views

Can low melatonin levels and/or LED lights cause or exacerbate macular degeneration?

I have two questions that may or may not be interconnected. My first question is: Does melatonin prevent macular degeneration? Could low melatonin levels (caused by blue-rich light exposure in the ...
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45 views

Antibody staining

I am staining tissue sections and I did a mistake, I was supposed to have mixed 3 primary antibodies but I stained only with one of them. After the 1h incubation I washed 10 min with PBS and then I ...
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54 views

Measurement equation of Tree-Step pathway model

I am not specialist in biology but I want to test a parameters estimation method (based on Kalman filter) on the Tree-Step pathway model, but I cannot find a document that gives the measurement ...
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37 views

Effect of the Marcus Theory on Photosynthesis

As far as I know, the Marcus Theory explains the rates of electron transfers in reactions as a function of differences in free energy between the product/reactant's potential well. Furthermore, there ...
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43 views

Why can polar molecules like water pass aquaporin better than unpolar like H2 or CO2

We learned in our lecutre, that aquaporin lets Water pass, but blocks Ions like H+ and unpolar molecules like CO2 and H2. The reasons for the Ion blocking are comprehensible, but I dont get, why less-...
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Preparation of dihydroxyacetone phosphate from glycerine by fermentation

I read on Wikipedia that dihydroxyacetone (DHA) can be obtained from fermentation of glycerin. I would like to obtain dihydydroxyacetone phosphate (DHAP) for a science experiment but, since I'm 15, I ...
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103 views

Origin of melatonin: oxidative stress defense OR circadian rhythm?

Question Which functional role of melatonin came first? Regulation of the circadian rhythm Defense against oxidative stress Other Background I was well aware of the important role melatonin plays ...
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63 views

Regulation of the concentration of precursors of DNA and RNA synthesis

What is the specific cellular process for regulating the amount of the four DNA bases (adenine, thymine, cytosine, and guanine) that are present in the cytoplasm of a cell? Basically, how do the cell'...
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46 views

What are some good examples of phonotaxis in plants and other inanimate beings?

Reaction to the sound waves by plants is commonly unobserved. A physical movement or chemical release examples due to phonotaxis would be good to know about.
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199 views

Modulating the level of acetyl CoA

Describe what might happen if there were no mechanisms for modulating the level of acetyl CoA. I am not sure what is meant by "modulating the level of acetyl CoA", or what mechanisms contribute to ...
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53 views

Could trees be engineered to produce rainfall-nucleators?

Scientists are using genetic engineering to make trees produce more terpenes. Is it possible to genetically engineer trees to produce more hygroscopic volatile organic compounds? These trees could be ...
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124 views

Why does a broad-leaved evergreen (Mahonia aquifolium) has red leaves?

To my knowledge, oregon grape (Mahonia aquifolium) is purportedly an evergreen shrub? Deciduous broadleaf plants lose their leaves in autumn usually and before that as the leaves die they oxidize and ...
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44 views

Where do class 2 preservatives end up after digestion?

Do anybody know what happens to class 2 preservatives in general after they are digested - i.e details like weather they are absorbed into blood as simplest elements or excreted out or assimilated to ...
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126 views

Does alpha-amylase from different species have different *optimal* conditions?

Do the optimal conditions for the enzyme isoforms differ between species? Specifically, do the optimal pH and temperature for alpha-amylase differ for that enzyme produced by B. lichiniformis and A. ...
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180 views

What is the function of CETP?

I read up that CETP transfers cholesterol from HDL, which collected it from tissues, to VLDL. This VLDL is then sent back to the tissues, ultimately forming LDL internalised by cells. What is the ...
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72 views

What Chemical Trigger Causes Ectomycorrhiza To Change From Asexual To Sexual?

I want to know the trigger behind the change of asexual to sexual ectomycorrhiza when symbiosis with a tree root is formed. As ectomycorrhiza attaches itself to a root, it forms a relationship wherein ...
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689 views

Can I leave BL21(DE3) cells in room temperature?

I am preparing competent cells, and I finished inoculating a single colony in SOB. It has been incubating at 37 degrees Celsius for almost 16 hours since, and it's getting very late where I live. I ...
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46 views

Does blood typing still provide a use for ancient tissue analysis?

Modern techniques. In recent years, DNA sequencing has become extremely cheap. This, compounded by the ability to PCR miniscule samples to viable samples for analysis, means that aDNA can be ...
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230 views

How does estrogen influence collagen synthesis?

Through what mechanisms does estrogen interact with collagen synthesis? Especially in the context of elevated estrogen levels and genesis of purpura simplex .
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21 views

Why might a cell have no respiratory reserve capacity?

I am trying to understand why a cell might have minimal respiratory reserve capacity in the presence of unlimited substrate supply. This essentially means that the oxygen consumption rate (OCR) of the ...
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34 views

Why does pyruvate from lactate and pyruvate from other sources follow different pathways in gluconeogenesis?

My teacher taught me in a lecture that PEP forms from Pyruvate by two ways, based on their sources, that is - 1. If the Pyruvate was from lactate (by lactate dehydrogenase action), it gets shuttled ...
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50 views

How do molecular biologists determine biochemical pathways?

I'm new to this community, so hopefully this is the right place to ask this question. I know my question is really general, but in all of my biology courses we are merely taught the chemical pathways ...
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296 views

Why does GLUT2 (With a high Km), bind less glucose at a lower glucose concentration (below Km)?

A text I am reading says "GLUT2 is a low-affinity transporter in hepatocytes and pancreatic cells. After a meal, blood travels through the hepatic portal vein and GLUT 2 captures excess glucose for ...
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92 views

Is starch a better coagulant than cellulose?

In an experiment, I used plantain peels (with a very high starch content and very little cellulose) and grapefruit peels (with a very high cellulose content and no starch) as coagulants (ensuring that ...
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57 views

Can we investigate the cell biology of extinct microorganisms?

I know that there are microfossils, but not if we can we know about the organelles, genetics or macromolecules of the cells that left them behind.
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38 views

Sucrose inhibit onion root mitosis? Why?

I am doing an experiment about onion root growing. I had put an onion in different solutions of $C_{11} H_{22} O_1$ in H2O —with the root touching the water—: 0,1M; 0,2M; 0,3M; 0,4M; 0,5M. The ...
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72 views

Would I expect salt precipitate on fibres of DNA in a NaCl water solution?

I just recently conducted an experiment in my biochemistry class where we had to add DNA to distilled water, isotonic saline and 2.5M NaCl. I noticed different physical aspects depending on the ...
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What is the concentration/molecules per cell of iodothyronine deiodinase in a cell?

I'm trying to parameterize an in silico model and need the approximate concentration (M), or molecules per cell, of iodothyronine deiodinase type II (D2) enzyme in a human cell, ideally a glial cell. ...
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37 views

What happens to the brain during meditation?

I've read several experiments on the internet according to which it is possible to reach a psychedelic state without taking any psychedelic drugs like DMT, LSD and other tryptamine derivatives. It ...
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99 views

Why do plants produce and store both amylose and amylopectin?

Since both forms of starch has its primary purpose of storing glucose and hence releasing energy, why are there 2 variations of this sugar? Is it possible for an organism to contain/depend only on 1 ...
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122 views

Is there any way to differentiate between a contractile and a non contractile vacuole by observation alone in any given organism

I have read about distinguishing them using osmotic experiments in books and here as well. But is there any thing common (on a biochemistry level) from which I can tell if a vacuole is contractile or ...
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72 views

What happens during fixation of Nucleic acids on nylon membrane

In Southern blotting technique, fixation of DNA to nylon membrane is a step prior to hybridisation with probe. It is done by baking at 80ºC and according to this cross linking between DNA and nylon ...
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43 views

What do we mean when we say that a muscle fiber contracts strongly?

Sounds dumb but anyway, i heard that superfast fibers contract more strongly than fast twitch fibers. And i never thought of muscle contraction as something that has a magnitude (i thought of it as "1 ...
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322 views

In what reaction of mitochondrial respiration is hydrogen peroxide an anomalous product?

I understand that hydrogen peroxide is produced in an anomalous manner during mitochondrial respiration, which also produces other by-products or free radicals. Does anyone know the details of the ...
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91 views

Can NADP be converted to NADPH using d glucose?

For one of our reaction, we require NADPH enzyme. We have NADP in our stock. Is it possible to convert NADP to NADPH using D-glucose anhydrous and if so please provide me how to go about it?
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96 views

Artificial Hormones of human body

Can hormones of human body created artificially even i mean the hormones just consisting of bio chemicals and nothing else?
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0answers
76 views

When can helices form coiled-coils?

The other day, my teacher suggested we find out whether a chain of the following amino acid sequence would be able to form a coiled-coil: N-KIAEVRAQYEDVANKVRLIVE-C ...
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55 views

Where am I going wrong in a Reactive Oxygen Species Assay done in N9 cells?

I have followed the modified protocol that was given to me by my senior (who was using for his RAW 264.7 cells - Reference article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3308605/ ) for ...
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0answers
94 views

Why do basil leaves turn black?

I notice that when I buy basil it is fresh and green in the store, but when I take it home it wilts and turns black within 24 hours. Why does it do this?
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0answers
91 views

How well can proteins discriminate between ATP and GTP? Can ATP act as a GTP mimetic?

GTP and ATP are similar structures with the adenosine and guanosine groups differing. Both are involved in a vast array of biological functions. However it has been shown that in certain cases, GTP ...
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45 views

Looking for dataset of proteotypic and non-proteotypic peptide

I doing experimentation for peptide prediction using machine learning. I need some data for testing. My background is Computer Science. Any advice how to find proteotypic and non-proteotypic peptides....
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0answers
40 views

How do organoarsenics improve digestion efficiency in poultry?

It struck me as very surprising that these organoarsenic compound with structure looking not very compatible with living system is widely used as food additive to increase weight gain and improve food ...
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139 views

Can aptamers be used to identify the terminal amino acid residue of a peptide?

Suppose there were DNA or RNA aptamers that bind specifically to a certain terminal amino acid residue. Let's name it aaSA (amino acid specific aptamer). For example, the aptamer binding to terminal ...