Questions tagged [biochemistry]

The study of chemistry within the scope of biology: the compounds that occur and the reactions involving them in living organisms.

Filter by
Sorted by
Tagged with
0
votes
0answers
30 views

Why don't our tongue receptors for salt and sugar adapt to them, like the ones for pepper do?

Many (most?) physiological receptors adapt to the substance they bind to, leading to higher dosages required to elicit the same response. In pharmacology, it’s called “drug tolerance”. In physiology, ...
0
votes
0answers
21 views

Do chilli peppers change in composition after they change color?

I grow some chilli peppers and was wondering when the time is right to harvest them. Of course gardening-focused sources say that they are ripe when they change color. This usually happens very ...
0
votes
1answer
48 views

Is there anyway from which we can measure the viscosity of honey using a refractometer?

Is it possible to use a refractometer to measure the viscosity of honey because as we know, a refractometer is used to measure moisture and therefore water influences the viscosity of honey so is ...
2
votes
0answers
24 views

How it will taste if one puts two substances on tongue simultaneously?

If we put two substances having different taste on tongue simultaneously how it will taste? Does specific part of tongue is sensitive to specific taste?
6
votes
1answer
93 views

Are there any known consequences of the right-handedness of the DNA double helix?

In this article it is suggested (without evidence) that the right-handedness of DNA may be the cause that "kick[s] off asymmetry in the early embryo [of snails]". On the one hand we know that ...
3
votes
2answers
167 views

Reasons why this protein is not suitable as an immunogen?

In a paper entitled "Progress and Prospects on Vaccine Development against SARS-CoV-2", the authors write the following in section 2.5: "Compared with S, N, and M protein, E protein is not suitable ...
3
votes
1answer
50 views

What's the role of bromelain in pineapple?

Bromelain refers to one of two proteases found in pineapple and its relatives. Like other proteases, many believe it has therapeutic uses and it's the subject of a lot of research. But what role does ...
0
votes
0answers
20 views

Basic Molecule Editor for use in journals / theses?

My background is Geosciences and Physics and I am going to study water and biomolecules in the Autumn. I was reading a PhD thesis about peptides and peptoids by someone in my research group and I had ...
2
votes
0answers
45 views

Does it make sense to express membrane transporters as concentration?

Let's assume a situation where a molecule, S, is transported out of the cell by membrane transporter T. For simplicity we do not consider any other synthesis or production processes. Furthermore, we ...
2
votes
1answer
59 views

How does exercising/starved muscle import glucose (released by liver)?

Adrenaline releases glucose from the liver during sport or if starved. This glucose goes to the blood through GLUT2 transporter. But how does it get transported into the muscle cells? GLUT4 is the ...
2
votes
0answers
17 views

Can muscles remake pyruvate from lactic acid, or does it have to go to the liver in the Cori cycle?

When the muscle is exercising, and only anaerobic respiration is done, pyruvate -> lactate to regenerate NAD+. Lactate is then transported out of the muscle and into the liver, to regenerate glucose, ...
2
votes
0answers
11 views

What relation do glycerol-3-P DH and acyl-CoA DH have with Complex II of the ETC?

I am sorry if this may be a purely definitional/nomenclature question. Complex II of the electron transport chain (ETC) would be succinate dehydrogenase, transporting electrons to ubiquinone (and ...
2
votes
0answers
104 views

What makes the cytoplasm a reducing environment?

It is known that the cytoplasm is a "reducing" environment, where disulfide bonds cannot form (will soon be reduced to 2 cysteines) [I'm not putting a link as this is a fact in many biology textbooks]....
2
votes
0answers
11 views

Does an FAD:FADH2 ratio exist in the cytoplasm? (similar to NAD+:NADH ratio?)

I have learned about a lot of enzymes/proteins which are covalently bound to FAD, and use this as an oxidising agent. In vivo, FAD is (almost) always protein bound (very low concentrations of free FMN/...
2
votes
1answer
51 views

Sickle-cell trait : Why is only one type of beta chain found in any one hemoglobin molecule?

I was reading a textbook (iGenetics, 3rd edition page 71), and came across the following passage: Homozygous bS bS people make Hb-S, the defective hemoglobin, with two normal a chains specified ...
2
votes
1answer
98 views

Base-stacking interactions versus hydrogen bonds

Is it correct that the hyperchromic shift in DNA is a result of the disruption of the base-pairing hydrogen bonds, but the actual denaturation of dsDNA has more to do with the base-stacking ...
0
votes
0answers
19 views

Overcoming palmitic acid intolerance

What can stop the body from being able to process or break down palmitic acid? The acid stays in the stomach and burns causing a similar reaction as heart burn when the volume is high. Heart burn ...
0
votes
1answer
59 views

Is there any free software similar to CellDesigner that allows me to represent large networks of biochemical reactions in the human body?

I continually have to review multiple articles, in multiple journals. These articles present how various enzymes, proteins, lipids, carbohydrates, etc. intervene in the processes that lead to certain ...
-2
votes
1answer
64 views

Why is it that evolution of organisms had oxygen as their life-supporting gas?

On Earth, gaseous N2 is abundant. However, life began by dealing with O2 and CO2. What can be considered the major reason for life developing in such manner?
1
vote
1answer
154 views

What causes the localization of myoglobin in turkey to regions of muscle tissue?

I've read that myoglobin localization is responsible for the darker colour of leg muscles in turkeys. Why does this localization occur in terms of any of cell biology, molecular biology, or ...
2
votes
1answer
93 views

Growing Mouth Bacteria in a bowl of sugar water

I want to teach my nephew why brushing in necessary and how sugar in mouth reduced by bacteria to acid. I plan to put sugar mixed water in bowl and take swab from his mouth and dip in bowl, and test ...
0
votes
1answer
55 views

Are there any “thermotroph” lifeforms?

I only found chemotroph and photoroph examples of life, but are there organisms that can use a temperature gradient to convert energy?
0
votes
1answer
293 views

Is touching or inhaling 70% isopropyl alcohol dangerous to humans?

I bought 99% isopropyl alcohol and made 70% alcohol + 30% water in small spraying bottles as a disinfectant. I also read that this solution is safe and recommended by Apple for cleaning iPhones. My ...
2
votes
3answers
121 views

Do proteins contain phosphorous? If its true then how alfred hershey and martha chase used the statement that proteins do not contain phosphorous?

I hv heard about phosphorus that it is a constituent of certain protein, although we know that no amino acids have phosphorus... and if its true then how Alfred hershey and Martha chase experiment got ...
0
votes
1answer
97 views

Can mold grow on copper pipes?

Couple months ago noticed something was literally, for lack of a better term, eating paint off of lead pipes. And I noticed joints in copper pipe seemed to be "leaking" flux. Now all the water in my ...
2
votes
2answers
55 views

Can catecholamines degrade back into tyrosine, or, is synthesis irreversible? (in human body)

Catecholamines like dopamine, noradrenaline and adrenaline are broken down with enzymes that catalyze the reaction. Can they degrade back into tyrosine (a conditionally essential amino acid), or is ...
1
vote
0answers
25 views

What causes the overactivity of keratin production and expression in the cells of the nail bed/ nail matrix after some traumata?

What is the cellular mechanism of traumatic(sterile) onychodystrophy(hypertrophy)? I.e one hits his toenail and has it removed twice( once after the first trauma and then again 3 years after the first ...
1
vote
0answers
38 views

how can we identify the novel protein amino acid sequence and function? [closed]

if there is a novel protein, how would you know the amino acid sequence and how would you determine the number of subunits and the function of that protein? Specifically what molecular techniques we ...
2
votes
1answer
17 views

Values of the blood tests pancreatic amylase and lipase

Anyone know what is considered slightly elevated, moderately elevated and strongly elevated values of pancreatic amylase and lipase? Many different laboratories in my country divide between these ...
1
vote
0answers
24 views

Toxicity of stannous fluoride in toothpaste?

I recently purchased a new brand of toothpaste, and when I looked at the medicinal ingredients, it listed stannous fluoride 0.454% w/w, instead of the regular sodium fluoride. I was a bit concerned ...
1
vote
0answers
27 views

Examples of alkali metal and halogens covalently bonded in biological molecules?

Divalent and monovalent ions are often non-covalently bonded in biological molecules, they are primary building blocks of the machinery of biology. Iodine, the halogon in period 5, is covalently ...
0
votes
0answers
26 views

Does the zeta potential of a nanoparticle generally take into account ligand charge?

For example, if I had a quantum dot nanoparticle with conjugated linker peptides capped with polyarginine tracts. Would the localization of negative charge from arginine change the zeta potential ...
0
votes
1answer
49 views

Why doesn't copper affect human skin?

It's been known for a long time that copper has antimicrobial properties, but if it is so potent, why does it seem to have no effect on human skin or really any large animal?
2
votes
0answers
27 views

Why does Porous-tube Nutrient Delivery System cultivation improve nutritional quality of Okinawa spinach (Gyruna bicolor)?

I wrote a review paper and it involves in growth system of a plant “Okinawa Spinach (Gynura bicolor)”. A research of (Wang, Fu, & Liu, 2015) reported that Porous-tube Nutrient Delivery System (...
4
votes
3answers
19k views

Ratio of bleach to water required to disinfect COVID-19?

I am confused about the needed ratio of bleach to water required to disinfect COVID-19 from surfaces. The guide on CDC states that: https://www.cdc.gov/coronavirus/2019-ncov/community/home/cleaning-...
1
vote
1answer
46 views

Relationship between bacteria size and temperature?

I'm, doing a research in a TOC (total organic carbon) degradation in a BAC (biologically active carbon) filter for the grey-water treatment. Recently, I started to wonder if there is a relation ...
3
votes
1answer
147 views

Why is Ibuprofen contraindicated in asthma patients?

So yesterday a patient showed up at the clinic with a massive swelling in his left face region. Upon examination it was found to be due to infected first premolar. Dentist recommended him to get the ...
2
votes
1answer
46 views

Does the composition of a meal play a big, or small, role in food's thermogenic effect on the body?

Reading in: "Diet and Health: Implications for Reducing Chronic Disease Risk / Calories: Total Macronutrient Intake, Energy Expenditure, and Net Energy Stores" [NCBI Resources]: "The thermogenic ...
0
votes
1answer
128 views

How are logarithmic trendline equations used to determine antibody concentrations in ELISA?

For example, say you have plotted a standard curve and managed to obtain a logarithimic trendline equation like: y = mln(x) - b And you are working with data that includes: a) standards,their ...
-3
votes
1answer
151 views

Is it possible to artificially induce a 40°C fever in a controlled and safe manner? [closed]

In search for any possible new treatments for COVID-19, I am of the understanding that the SARS-nCoV-2 virus may suffer deterioration at high temperatures and high humidity, to perhaps weaken it ...
0
votes
1answer
54 views

Circulating Tumor Cell vs Circulating Tumor DNA

I'm a little confused about the wording of these two phrases and under which context the epithelial-mesenchymal transition occurs. For example: Is it the circulating tumor cell that releases the ...
-1
votes
1answer
108 views

How to analyze phosphorylation shift by western blot?

I want to see the phosphorylation shift in my protein of interest. I have created a point mutation in my protein. so that it will not able to go for the phosphorylation compare to my control. i want ...
1
vote
1answer
25 views

How exactly can a drug affecting squalene epoxidase affect the methylation cycle, necessitating the use of 5-MTHF?

I found a case report in which the authors say that Terbinafine might have possibly affected the methylation cycle in a patient, necessitating the prescription of 5-methylfolate to correct the ...
1
vote
0answers
38 views

Rescuing a virus from naked (c)DNA

Reading the articles it seems that for several human virus strains, synthesizing the full-length genome then electroporating into vero cells and incubating a few days gave birth to living viruses that ...
5
votes
1answer
134 views

Tryptophan side-chain structure, how should it be orientated?

I would like some clarification on whether it matters where the NH and double bond are placed in these two structures of tryptophan (W) that I drew. I marked the right side as correct and the left ...
2
votes
0answers
45 views

What happens when you cook tree spinach with aluminum?

The Internet is filled with warnings that you shouldn't cook tree spinach(Cnidoscolus aconitifolius) in aluminum, because it will react and create toxins that cause explosive diarrhea when consumed. ...
5
votes
3answers
176 views

How can one relate in vitro studies of caffeine (dose expressed as concentration) to dietary intake of caffeine (dose in mass)?

Having difficulty making the data in the below charts to apply to daily life. What is the right interpretation and implementation? How are the “in vitro” results of the below charts relevant to levels ...
3
votes
1answer
51 views

Bimodal frequency distribution of size of protein loops

The graph of number of amino acid (AA) residues in a loop Vs the frequency of their occurrence in proteins largely follows a tending-to-zero pattern. However, there appear to be some specific number ...
2
votes
1answer
59 views

In vivo, does it take energy to move double bonds from cis to trans in long chain unsaturated fatty acids

In vivo, I understood that because all polyunsaturated fatty acids bonds are -cis they are crowded, kinking the molecule, and because of that, the oxidative enzymes cannot initially access them. They ...
1
vote
0answers
28 views

About the mechanism of coupled reaction/metabolism with ATP

I am not in the field of biochemistry so this may be a rookie question or misconception. I heard occasionally about the energy "released" from ATP hydrolysis fueling (endergonic) biological reactions....

1 2 3
4
5
38