Questions tagged [biochemistry]

The study of chemistry within the scope of biology: the compounds that occur and the reactions involving them in living organisms.

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27 views

Do pyrocalciferol and isopyrocalciferol form in vertebrate skin?

I heard that pyrocalciferol and isopyrocalciferol can only be formed above 100°C/212°F, yet some images in the web depict them as forming in vertebrate skin. So, I wonder if pyrocalciferol and ...
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48 views

Does food grade diatomaceous earth contain mercury

Not sure if this is the right site to ask this question, but since diatomes are or once were biological creatures it seemed to fit this one more than any of the others. I have been supplementing my ...
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20 views

Coagulation of protiens of different products

Protein of egg coagulates on 60 degree but proteins of milk do not coagulate at high temperatures..why? What is the main reason behind all this?
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110 views

Ligand-binding assays: IC50, EC50 and Kd

I am reviewing several MHC-peptide binding affinity predictors trained on IEDB data. Quantitative records for MHC class I allotypes come from a lot of different assays and, by extension, have ...
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52 views

What enzyme or bacteria could dissolve plastic?

What enzyme or bacteria could dissolve plastic aside from acetone? Preferably, low-cost and environmentally friendly. Any suggestions would be helpful. I'm targeting for thin plastic (low-density ...
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32 views

How to troubleshoot in vitro formaldehyde fixation for nucleosomes?

For an experiment, I am trying to fix the mononucleosomes (100ng) using formaldehyde as crosslinking agent in HEPES buffer. I have been using 2% formaldehyde in a reaction buffer containing 1mM EDTA, ...
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278 views

Does cat urine really fluoresce? If so, why?

I temporarily got caught in the Sad and Useless website, and found Science is Fun. Most of the claims seem plausible, but I can't guess why cat urine would fluoresce. Comments have directed me to ...
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69 views

What is the difference among biochemistry, molecular biology, molecular genetics and structural biology?

I have never heard of straigthforward definitions of these fields in my college lectures, and the Internet searches were not very helpful. However, from what I have learned at different subjects, this ...
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130 views

The effect of salt, pH and temperature to the salivary amylase

If I prepare the following set of tests, incubate for 15 mins, then add 2-3 drops of iodine solution to each test tube: Salt test (1ml starch solution + 0.5 ml enzyme + 1ml 1M NaCl, incubate at 37 oC ...
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138 views

Does our brain use up more ATP after smoking cannabis?

kind of an amateur here. If the firing of the neurons' signals uses up some ATP, and smoking cannabis makes them fire off more quickly, do our brains use up more ATP to sustain that rapid firing?
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30 views

What is the composition of a standard diluent buffer in a leptin ELISA kit?

I am trying to find the composition of the diluent buffer used for the dilution of a human leptin stock to be used in an ELISA assay. The manufacturer of the kit (https://www.thermofisher.com/elisa/...
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17 views

Coacervater were more like Viruses or Cells?

The answer considers them as first formed living cells as they were precursor of life. But they had no lipid bilayer nor any cellular organization, shouldnt they be more like Viruses ,or like Prions ...
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116 views

How does the body differentiate between sources of good and bad cholesterol?

I keep hearing "good cholesterol, bad cholesterol" everywhere, and how certain food sources of cholesterol raise LDL and certain other raise HDL. I don't understand how any food can increase ...
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52 views

Why is plasma glucose concentration not double that of whole blood?

It is known that the concentration of plasma glucose is 12% higher than that of whole blood. But since 45-50% of whole blood is red blood cells, shouldn't the plasma glucose be almost double — since ...
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36 views

What is the difference between GFP and rhodopsin?

GFP and Rhodopsin are both fluorescent proteins. What is the main difference between GFP and Rhodopsin in terms of how they work? I know that their excitation and emission wavelengths are different, ...
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76 views

What is the most readily usable form of nutrients/energy source for heterotrophic microorganism in general?

Consider a nutritious mixture containing abundant soluble sugars, soluble lipids and soluble protein etc. Would heterotrophic microbes preferentially use sugars first > lipids > proteins > insoluble ...
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123 views

MOPS Media Recipe Protocol - Well Defined

I am trying to find a clear protocol for making MOPS media. However, when looking online, I am finding that this media is loosely defined and I can not find a good protocol. (I have been looking for a ...
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54 views

What biproducts do vinegar eels produce?

What chemicals, acids, gases or other biproducts do vinegar eels (Turbatrix aceti) produce (in raw vinegar and/or in the vinegar-creation process, which process may have them in environments including ...
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214 views

Is dietary fat directly stored in adipose tissue or is it immediately metabolized?

I know that fat goes to adipose tissue and even sugar gets converted to fat and goes to adipose tissue, but what happens to fat (unsalted cow milk butter) I just ate? Will it give me energy now, or ...
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174 views

Diazepam with Aluminum Hydroxide

Some common medicinal preparations contain diazepam with aluminium Hydroxide, there may be other compounds [e.g. magnesium trisilicate and polydimethylsiloxane with Aluminum Hydroxide and Diazepam as ...
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98 views

peritoneal dialysis Vs Hemodialysis?

Their are basically two types of dialysis: 1.peritoneal dialysis 2.hemodialysis, My Question is Why the peritoneal dialysis needs to be carried out on everyday BUT hemodialysis is carried out on only ...
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35 views

Amount of Na+ needed to be transfered in order to depolarize the membrane?

I found out that the number of $Na^+$ ions that is needed to be transfered across the membrane to make it depolarized is a small number. In what way its proved? (Goldman equation maybe?)
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112 views

Does the source of caffeine affect the biological impact?

I've recently decided to give up my morning coffee in lieu of a caffeine pill. Both are around 200mg (my Tim Horton's XL 4x4 was 240 mg of caffeine) of caffeine. We were talking about the various ...
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109 views

Determination of Ageing by ECG inclusions/exclusions?

I am studying ageing and considering ECG signal because of its high sensitivity in theory (escardio). Some factors Sensitivity Gender Medical treatment ... Benchmark: RTG dental + wrist uncertainty ...
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46 views

Avoid Blood lyse color remain in DNA extraction

I tryed several times to extract DNA from freezed blood sample whit Qiagene kit but it gave very small amount of product due to good purity then I try extract manually by using CTAB PVP method and ...
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125 views

Root etiology of non-pituitary low T4 and low TSH

Please note: I'd like to preface this question by stating that this is neither homework nor me seeking medical advice. I am simply trying to understand the biological, physiological root etiology ...
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52 views

Consumption of different type of energy when one is inactive

I'm wonder if there is any study done on this and what are the results? how much of each type of energy one's body(healthy normal person) uses when inactive(seating down) I know there are alot of ...
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26 views

Size of the glycan chain in a glycosylated protein

I would like to know where I can find the (experimental/estimated) distribution of glycan sizes attached to proteins.
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559 views

What is the probe that absorbs at 450nm in the presence of NADH in this assay?

The colorimetric assays by Biovision and Sigma Aldrich seem to utilise a probe that binds to or reacts with NADH in order to cause absorbance at 450nm which can then be quantified by a ...
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1answer
98 views

Can mold grow on copper pipes?

Couple months ago noticed something was literally, for lack of a better term, eating paint off of lead pipes. And I noticed joints in copper pipe seemed to be "leaking" flux. Now all the water in my ...
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1answer
73 views

Is α-keratin a fully functional protein?

Is α-keratin before it coils with another polypeptide, makes chains, and build intermediate filaments a fully functional protein? I mean, is the single monomer of α-keratin a protein or does it have ...
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38 views

what happens when you put enhancer and caat box in prokaryotes

what happens when you put enhancer and caat box in prokaryotes
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474 views

dNTP concentration for reverse transcription from RNA to cDNA

Does it affect the results if I use the half of dNTP amount in a reaction that it says in my protocol? I usually use 4 microlitres per reaction and this time I did a mistake and I used 2. The final ...
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1answer
114 views

Is it possible to stop more/extra fat from being stored in the body?

I don't mean simply by restricting eating or upping the activities, but a way to basically stop the body from adding more fat on itself down on the bio level. For example, I'm not too knowledgeable on ...
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26 views

Why do bile salts have non-polar binding to polar bits?

My textbook has this image of the mechnaism of bile salts. It states that bile salts are amphipathic. Thus, their hydrophilic end will face the water and their hydrophobic end will face inside. ...
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32 views

Where does the hydroxyl group on DNA/RNA phosphate group preceding phosphodiester linkage come from?

I have a curiously simple question that I have been struggling to find an answer to online. When I look at a standard diagram of a solitary nucleotide, the phosphate group contains two oxygens with a ...
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63 views

What is the point of lipoproteins if albumine exists?

Why do Lipoproteins (Apo B 48/100 , Chylomicrones, etc.) exist if Albumine is capable of transporting fatty acids? Why doesn't the body just transport everything via albumine?
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63 views

How can I liquefy egg shell membrane

I want to extract egg shell membrane from the eggs. And I want to put them together and turn them into fluid so that I can make whatever shape I want. Is there any method that can make them into fluid?...
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1answer
288 views

Difference between cerebroside and globoside

I have a general idea about their difference that cerebrosides have a single sugar while globosides have more than one sugars. This is the structure of a ceramide (syphingosine and a fatty acid ...
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1answer
17 views

Protein Pathways, Superfamilie, Function database/website?

I'm going trough some old data - but I'm running into this problem that all website we used use is not existing anymore. What I have is a very long list of proteins with accession number (refSeq from ...
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1answer
323 views

Can streptavidin be conjugated with EDC-activated carboxylates?

Recently I conducted an experiment to conjugate amine-modified oligonucleotides with hydrogel particles having carboxyl groups. However, the fluorescence (from hybridized FAM-DNA) only appears on a ...
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1answer
470 views

What are internal and external osmotic pressures when referring to a cell?

I've seen the term used but cannot find a clear answer: What are internal and external osmotic pressures when referring to a cell?
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1answer
57 views

can magnesium bicarbonate be absorbed in the mouth?

Can Magnesium Bicarbonate be absorbed in the mouth?" Magnesium Bicarbonate occurs naturally in some mineral waters.
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1answer
68 views

Can an RNA anneal to its complementary (not reverse) sequence?

Say I have a target RNA strand: 5'--AAUGGCCCUUUAAA-->3' I know that this strand will readily anneal to the sequence 3'<--UUACCGGGAAAUUU--5' which is the reverse-complement. I'm wondering if ...
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36 views

Is brown or black eumelanin more photoprotective?

Is black or brown eumelanin better at protecting against the sun's harmful ultraviolet radiation? I searched on the web for answers with no luck. I do know that pheomelanin is phototoxic and eumelanin ...
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1answer
33 views

How does lipoid pneumonia lead to acute respiratory distress syndrome (ARDS)?

How does lipoid pneumonia lead to acute respiratory distress syndrome (ARDS)? The vaping illnesses that have been happening on the news in the United States are being caused by the federal ...
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1answer
139 views

Why is the macromolecular composition of a human cell seemingly hard to find?

I am trying to find a 3D rendering or list of molecules by volume in a cell (originally I was searching for human, but I'll take any at this point!). I understand the open-endedness of the question: ...
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1answer
2k views

Types of heparin

My textbook(biochemistry 4th edition, U. Satyanarayana) says that the following structure is of heparin and it's name should be D-Glucuronate 2-sulfate, N-sulfoglucosamine 6-sulfate. I searched on ...
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291 views

Proinsulin is an 84 residue polypeptide with six cysteines. How many different disulfide combinations are possible?

Generally cysteine residues form disulfide linkages - so how many combinations are possible out of (say) six residues. Also can cysteine form bonds with all the residues?

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