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Questions tagged [biotechnology]

Biotechnology is a field of biology which utilizes biological systems like microorganisms to produce valuable products, such as biofuels, drugs, and vaccines.

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A plant that is used as antibiotic for trichomycosis axillaris bacterial plates

I want to know a plant may be known as medicinal to treat as an antibiotic for the trichomycosis axillaris bacterial plates
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When linking a foreign DNA to a vector DNA why do we need to link it to the origin of replication

The entire DNA is going to replicate if replication takes place so why is it a necessity to link it exactly besides the origin of replication?
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Why does the material of the culture dish matter in animal tissue culture?

As we know that most animal cells are anchorage dependent cells, so they form a monolayer only when a suitable substrate is provided to them.. When a semi solid nutrient media is prepared and cells ...
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Transformation of a multicellular organism

I was reading about using RNA-interference as a method of introducing pest-resistance in plants. In tobacco plants, using Agrobacterium vectors, genes specific to the nematode Meloidegyne incognitia ...
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Primer design: Calculating free energy for secondary structure

I'm attempting to automate primer design (specifically, primers for RPA, which requires longer primers [~30-35 nc] than PCR), and need to figure out how to calculate the free energy of a primer pair ...
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Is it possible in modern biology to make any person's identical twin (or genetical clone) using his DNA?

I was told by a Biotech professor (who came in our college for a guest lecture) that Bio Technology has now become so advanced that if we want to make an identical twin (or Genetical DNA Twin) of a ...
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Protein electrophoresis

Three proteins A, B and C of equal molecular weight are being investigated in a study. They contain six, four and four cysteine residues respectively. Only Proteins A and B were treated with β-...
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1answer
40 views

What is the role of CRISPR-dCas9 in gRNA-dCas9 transcription regulator complexes?

In this paper, I read that mutant versions of Cas proteins such as a deactivated Cas9 (dCas9) are used alongside a guide-RNA (gRNA) to form variants of CRISPR tool that can function as transcription ...
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How do biologists estimate that there is 8% of protein coding genes in human genome?

In class, the professor said that there is about 8% of protein-coding genes in human. I wonder how biologists estimate this without annotating all of human genes. Thank you!
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What promoter is required for expression of a human protein Y in bacteria?

This is the actual question. It's from an entrance test for a research institute: A scientist wants to express human protein Y in bacteria. For effective expression of this protein he should use (A) ...
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Finding out the best concentration for my plant extract to be used as drug for diabetes

I am using plant extract of Ajuga parviflora and found out that it possess anti-diabetic properties by using alpha-amylase inhibitory assay. I used various concentration 250 µg/ml(29% inhibiton of ...
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Why is PCR possible to amplify DNA template before Sanger Sequencing for unknown DNA sequences?

Most of the informations show that both PCR and cloning can be used before Sanger Sequencing to amplify the DNA template. But how is it possible for us to design the primers if we don't know the DNA ...
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I haven't seen any gel lanes

I haven't seen any gel lanes in neither my PCR samples nor the marker itself when photographing them. This is not my first time performing 1.2% agarose gel electrophoresis. I see gel lanes when I ...
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What are the meaning the nomenclature in high cell density cultivation for the production of a protein? [closed]

I'd like to know the meaning of these terms in a HCDC studies of a modified strain. Max specific yield (mg/g DCW) Max volumetric product yield (g/l) Specific product formation rate Qp (mg/g DCW/h)
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Using nanodrop for analysing biological samples other than nucleotides

I am a 3rd timer postdoctoral fellow with some experience in molecular biology and biochemistry, but major skills in Zoology and Natural History. I am studying some natural extracts, and isolating ...
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1answer
30 views

What does ensemble-based model of enzyme mean?

I am reading Pan et al. (2000), a paper about dihydrofolate reductase (DHFR). They claim using a ensemble-based model of DHFR. What is a ensemble-based model?
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A doubt in biotech question

Which type of gene does not occur naturally in living organisms? Bt gene Endogenous cytoplasmic defense gene The answer is 2. I can't find out what this gene is! Does it mean the gene for ...
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Selection of recombinant host by color selection method

In recombinant DNA tech, To select a recombinant host from non-recombinants, we have different types of techniques available. The one I'm talking about is the color selection method with the use of ...
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What medium is the bacteria used to synthesize B12 sourced from?

Would that be from water, soil, plants, funguses,...? I asked the question in Quora but that, at the time of posting in this forum, haven't been very helpful. I also have searched for the ...
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What is the difference between Leloir and Non-Leloir glucosyl transferases?

The following questions were on the slides for my biotech course and I haven't been able to find any information on what the answer is. What is the difference between Leloir and Non-Leloir glucosyl ...
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What would be the molar concentration of human DNA in a normal human cell? [closed]

A diploid human cell has 46 chromosomes. A haploid cell has DNA approximately 3.2 billion bases long. What is the molar concentration of DNA in the cell then? How would we calculate?
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What is the difference between tissue engineering and 3D bioprinting?

3D bioprinting is the modified version of the tissue engineering but when we want to describe the both terms, what are the basic criteria or points to make difference between these?
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What are challenges faced by algae bioreactors?why they are not deployed on large scale if effective?

I was going through and studying how algae bioreactors work. I am mostly working for closed systems like tubular photobioreactors. I want to create bioreactors to combat pollution problems. However, I ...
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Gel electrophoresis bands

Is there any way to calculate the number of bands formed due to sodium dodecyl sulphate-polyacrylamide gel electrophoresis for a particular compound of group of proteins? For example, how could I ...
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232 views

Why does DNA need to be pure for restriction enzymes to act on it?

My textbook states: “In order to cut the DNA with restriction enzymes, it needs to be in pure form, free from other macromolecules. Therefore DNA isolated from other chemicals of the protoplasm like ...
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Mathematical Biology Jobs/Prospects? [closed]

I'm a junior math major starting to take his first courses in Algebra, Analysis, Topology, Nonlinear and Partial Differential Equations, etc. I am just considering my career options at this point ...
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Which ICs can I use to amplify electrical signals from plants?

I wanted to record variation potential and action potential generated in plants due to some stimulus. So which are the ICs that I can use for the amplifying the signals and what kind of experimental ...
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243 views

Estimated cost for diy biohacking [closed]

How much would it cost (more precise the better) if i wish to start a diy bio lab in my own house. Assuming that all I want is a functional set and to create maybe simple things like glowing bacteria/...
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107 views

What biotechnology can be used to treat lazy eye, and possibly cure lazy eye? [closed]

I tried very hard researching and finding the root cause of amblyopia. It is mainly because of imbalance in eye muscle. But, there are a lot more complicated causes of this disease. Thus, I need some ...
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How can I describe running a solution four times through a chromatography unit (in an instruction)?

I'm translating a document from Russian: The mean retention time for the first four injections of RNase should be (40.8± 2) min. If the mean RT is beyond this range, adjust the gradient system (...
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450 views

What is synaptic bias?

In non linear model of a neuron there was a mention about bias (Bₖ) which was the summation of the synaptic weights. I want to understand what synaptic bias is and their application/use in neuronal ...
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1answer
389 views

Can I do an MS in biotechnology after a bachelors degree in chemical engineering?

I recently graduated from high school and now have to make a decision about my undergrad major. I am interested in biotechnology but my parents suggested that I should opt for a broad based degree and ...
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399 views

Fermentation of bananas [closed]

I may have access to huge quantities of freshly picked bananas. Searching the web, I have found that the sugar content of the average banana is 14g of fructose (could stand to be corrected on this) ...
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Was HindII the first restriction endonuclease to be extracted?

Background: Guided by wikipedia and pmc I found this paper by Hamilton O. Smith. and .Daniel Nathans. A restriction enzyme is a component of a restriction-modification ...
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Why is a gene for a fluorescent protein used rather than one for an enzyme that produces fluorescent molecule?

Green fluorescent protein (GFP) is a small protein that emits bright green fluorescence in blue light. It was first isolated from the jellyfish. The gene coding for GFP can be expressed in bacteria, ...
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Could a 3D Printed Plant Seed be Possible with Existing Technology?

The first genetically cloned animal was Dolly the sheep. As I understand it, this was possible because all that was needed was an embryo containing the cloned genetic material and a womb for that ...
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A Designer Virus

An article at Wikipedia describes color changes that viruses have produced in tulips (without any human intention). And another article (elsewhere on the web) describes viruses that were designed to ...
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1answer
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How can I list genes present on a given series of bands?

My question is related to this one. My interest relate to this deletion: del(7)(q11q36)[12] as defined by ISCN. I would like to access a list of the genes found on the bands 11 to 36 of the 7th ...
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1answer
307 views

What step of the cloning process requires an egg cell? Have alternatives been attempted?

In the famous Dolly the sheep an egg cell was required. Why was specifically an egg cell required compared to say sperm cells or any other cell types? Given that egg cells are not trivially harvested ...
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1answer
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Anomaly in growth of fungus in Potato dextrose broth.. turbidity is not seen in one flask

I have inoculated six flasks of 200 ml potato dextrose broth, one of which does not show any turbidity and the medium is clear with a fungal disc in it. All were inoculated at the same time and from ...
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Why is western blotting used to confirm positive ELISA HIV tests?

I know that when an ELISA test indicates a positive result for HIV, a western blot is done to confirm. When I search online for the reason why, all that I see is that western blotting is "more ...
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Questions regarding PCR

Background: From Genomes: The temperature of a PCR mixture is first set at 94 °C so that denaturation of DNA can happen. Then it is reduced to 50–60 °C, which results in some rejoining of the single ...
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Is PCR a DNA cloning technique?

According to Genomes PCR is A technique that results in exponential amplification of a selected region of a DNA molecule [in test tube]. DNA cloning is Insertion of a fragment of DNA into a ...
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Is it possible to intentionally limit bacteria's lifespan?

Say I have a species of bacteria. I want this species to only persist for a set number of generations, after which it simply stops reproducing. Is it possible to do this at all (with known genetic ...
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561 views

Mass production of bacteria

Is there a way to mass produce a certain species of bacteria? Answers I am looking for should not involve a Petri dish: I am only interested in protocols involving, perhaps, more efficient mediums to ...
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Adding the Propionyl-CoA Pathway to E.coli

So I'm trying to simulate the production of 3-Hydroxypropionic Acid with E.coli via the Propionyl-CoA Pathway. However I'm not really sure how the production in the pathway goes. I got the following ...
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Why is it good to use Glycerol as carbon source to produce intermediates in pharmaceuticals?

Why is it good to use Glycerol as carbon source to produce intermediates in pharmaceuticals? My best guess would be because it produces a lot of ATP per carbon. But I haven't found anything to verify ...
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How to keep activated sludge viable for a week?

I am working in biological waste water treatment and I need to collect some activated sludge samples and have them characterized through different methods. The characterization will take from a few ...
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Mechanistically, how does calcium chloride aid in bacterial transformation? [duplicate]

I am looking to get more information about the mechanism of the $CaCl_2$ in the bacterial transformation. I read online about the topic but I didn't found any reverent information on the topic, I don'...