Questions tagged [biotechnology]

Biotechnology is a field of biology which utilizes biological systems like microorganisms to produce valuable products, such as biofuels, drugs, and vaccines.

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What are the career choices to work in the field of increasing human lifetime?

I'm done with high school, I want to work in the field of increasing human life expectancy and stopping death altogether,what should be my education path to so?
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Do we come to know which allele is dominant by seeing family genration tree only?

I know that a Gene has Alleles (variation) and one is Dominant over Other i.e the Other Recessive. Then I got a Thought that How can we tell whether an Allele is Dominant or Recessive...... and I came ...
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how long would oxidation of ammonia solution to nitrates take?

A hot topic among amateur chemists is making nitric acid from ammonia. While not as useful, im interested in making nitrate salts from ammonia as well. A simple idea is to just infect ammonia solution ...
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DNA denaturation and Renaturation

If we denature dsDNA by heating it and then rapidly cooling it then what would happen? I read this question, where it was written that if we were given dsDNA sample which was completely denatured and ...
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Public fermentation/cell cultivation database?

I am wondering if there are any large collections of fermentation or cell cultivation data publicly available. I am interested in all cell types used within industrial biotech and biopharma, ...
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What does this mean?

The phenotypic ratio of dihybrid cross 9:3:3:1 can be derived as a combination series 3 yellow: 1 green, with 3 round :1 wrinkled. This derivation can be writtenbas follows: (3 round :1 wrinkled) (...
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Can I use a Flask Weight Ring to increase RPM in an orbital shaking incubator?

I am working on a device that goes on top of the flask. This reduces the maximum RPM that I can use. Therefore I am wondering if I can use a Flask Weight Ring for Erlemeyer flasks in a orbital ...
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85 views

Is it possible to reduce maturity time of plants with biotechnology?

I've heard of speed breeding which makes use of optimal circumstances in glasshouses and growth chambers. Is it also possible to shorten a plant's maturity time (i.e. the time it needs from being ...
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Increase in pH of CDM4mAb media

We are working with CDM4mAb media when prepared in small quantity (1Litre) and pH is maintained to 7.0-7.2 with 1M HCL or 1M NaOH further filtered with 0.2-micron filter and is stored at 2-8 degree ...
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83 views

Technologies that rely on evolution [closed]

It's possible that this is offtopic here; if so, please let me know. I'm wondering what technologies exist that rely on evolution being a correct model; I'd like to be able to offer examples of that ...
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What are the difficulties/challenges against developing a coronavirus vaccine?

Multiple groups of scientists are trying to develop a coronavirus vaccine but they are not yet being fruitful. What challenges or difficulties are there in the process that slowing down and/or causing ...
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Design primers for PCR from given DNA sequence

Given the sequence: 5’-ACTGACTATGTAGAA………GGCCCTAAGGGCCAA-3’ (1) You wish to do PCR of this dsDNA to add “overhangs” on the ends of it. On the 5’ end of you will put the restriction site for ...
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Why can sperm be used in DNA fingerprinting?

As far as I know, DNA fragments with different lengths can be separated using gel electrophoresis. And the fingerprints can be used for identification by comparing common bands. However, the haploid ...
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Why is an antibiotic-resistance gene required during transformation of recombinant plasmid?

In my biology textbook, it said that in transformation, the recombinant plasmid containing an antibiotic-resistance gene act as a 'selective marker'. That means that plasmid in transformed bacteria ...
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1answer
81 views

Are there any ways to force DNA-origami to create a geometric shape from non-nucleotides (not from DNA)?

I mean, can we create with the help of DNA-origami figures that would not consist of DNA, but, for example, carbon? Is it even possible?
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Fluorescence assays to identify protein concentration without adding a large peptide sequence?

I'm trying to find a way of tagging a protein with something visually quantifiable to track protein concentration through potential purification steps and screen for the most efficient such steps. ...
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37 views

What pH ranges are used in industrial scale fermentation?

When reading Hans-Peter Meyer, Wolfgang Minas, and Diego Schmidhalter (2017) Industrial-Scale Fermentation. Industrial Biotechnology: Products and Processes, First Edition. Edited by Christoph ...
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Why haven't humans been able to make blood producing vats?

There are people in hospitals in life threatening circumstances that need blood packages, and they fully depend on blood donors' blood. With our current technology, we have been able to "persuade" ...
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1answer
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How DNA probe binds

I am studying about southern hybridization now and I've a doubt.After the DNA has been fragmented using restriction enzymes and obtained on nitrocellulose blot , it is still double stranded (the ...
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What is the use of having a surrogate mother in animal cloning?

In animal cloning, an ovum is collected by an animal and processed to form an embryo after fusion, which is going to be inserted to a surrogate mother. So my question is that why can't the embryo be ...
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Problem with joining the gene of interest into the open plasmid when their lengths are not the same

Before the recombinant plasmid is obtained in recombinant DNA, the gene of interest is inserted into a linearized plasmid by DNA ligase. What happens if the length of the gene of interest is not the ...
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Why are there two abrupt changes in the genome sequencing price curve?

In the price curve of the genome, from NIH: NIH explained the abrupt drop at 2008 thusly: beginning in January 2008... the sequencing centers transitioned from Sanger-based (dideoxy chain ...
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How can parentage test be accurately done even though crossing over occurs?

As crossing over occurs when gametes are secreted, so when a zygote is formed after fertilization, the base pairs of DNA in the child should be different from that of the parents. So when gel ...
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1answer
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Can Cas13 be used with multiple crRNAs in the same reaction?

CRISPR-Cas13 equipped with crRNA (complementary to transcripts of interest) can be designed to target ssRNA transcripts in cells. Upon successful crRNA and ssRNA binding, a fluorescent domain on ...
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Transformation of a multicellular organism

I was reading about using RNA-interference as a method of introducing pest-resistance in plants. In tobacco plants, using Agrobacterium vectors, genes specific to the nematode Meloidegyne incognitia ...
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Is it possible in modern biology to make any person's identical twin (or genetical clone) using his DNA?

I was told by a Biotech professor (who came in our college for a guest lecture) that Bio Technology has now become so advanced that if we want to make an identical twin (or Genetical DNA Twin) of a ...
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Protein electrophoresis

Three proteins A, B and C of equal molecular weight are being investigated in a study. They contain six, four and four cysteine residues respectively. Only Proteins A and B were treated with β-...
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What are the obstacles to modifying an algae to secrete fuel?

I've been reading a bit about algae fuel. It sounds like people did some work and then decided it was too difficult. This link mentions Exxon's work: https://en.wikipedia.org/wiki/Algae_fuel#...
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What is the role of CRISPR-dCas9 in gRNA-dCas9 transcription regulator complexes?

In this paper1, I read that mutant versions of Cas proteins such as a deactivated Cas9 (dCas9) are used alongside a guide-RNA (gRNA) to form variants of CRISPR tool that can function as transcription ...
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How do biologists estimate that there is 8% of protein coding genes in human genome?

In class, the professor said that there is about 8% of protein-coding genes in human. I wonder how biologists estimate this without annotating all of human genes. Thank you!
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202 views

What promoter is required for expression of a human protein Y in bacteria?

This is the actual question. It's from an entrance test for a research institute: A scientist wants to express human protein Y in bacteria. For effective expression of this protein he should use (A) ...
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1answer
38 views

Finding out the best concentration for my plant extract to be used as drug for diabetes

I am using plant extract of Ajuga parviflora and found out that it possess anti-diabetic properties by using alpha-amylase inhibitory assay. I used various concentration 250 µg/ml(29% inhibiton of ...
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Why is PCR possible to amplify DNA template before Sanger Sequencing for unknown DNA sequences?

Most of the informations show that both PCR and cloning can be used before Sanger Sequencing to amplify the DNA template. But how is it possible for us to design the primers if we don't know the DNA ...
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I haven't seen any gel lanes

I haven't seen any gel lanes in neither my PCR samples nor the marker itself when photographing them. This is not my first time performing 1.2% agarose gel electrophoresis. I see gel lanes when I ...
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What are the meaning the nomenclature in high cell density cultivation for the production of a protein? [closed]

I'd like to know the meaning of these terms in a HCDC studies of a modified strain. Max specific yield (mg/g DCW) Max volumetric product yield (g/l) Specific product formation rate Qp (mg/g DCW/h)
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Using nanodrop for analysing biological samples other than nucleotides

I am a 3rd timer postdoctoral fellow with some experience in molecular biology and biochemistry, but major skills in Zoology and Natural History. I am studying some natural extracts, and isolating ...
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1answer
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What does ensemble-based model of enzyme mean?

I am reading Pan et al. (2000), a paper about dihydrofolate reductase (DHFR). They claim using a ensemble-based model of DHFR. What is a ensemble-based model?
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A doubt in biotech question

Which type of gene does not occur naturally in living organisms? Bt gene Endogenous cytoplasmic defense gene The answer is 2. I can't find out what this gene is! Does it mean the gene for ...
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1answer
123 views

Selection of recombinant host by color selection method

In recombinant DNA tech, To select a recombinant host from non-recombinants, we have different types of techniques available. The one I'm talking about is the color selection method with the use of ...
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What medium is the bacteria used to synthesize B12 sourced from?

Would that be from water, soil, plants, funguses,...? I asked the question in Quora but that, at the time of posting in this forum, haven't been very helpful. I also have searched for the ...
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What is the difference between Leloir and Non-Leloir glucosyl transferases?

The following questions were on the slides for my biotech course and I haven't been able to find any information on what the answer is. What is the difference between Leloir and Non-Leloir glucosyl ...
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142 views

What is the easiest way to measure a human body surface area? [closed]

Looking for accuracy, cost-efficiency, and speed: this could provide useful information for determining individual bioinformatic calculations, pertaining to, for example cell type estimation, for ...
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3k views

What would be the molar concentration of human DNA in a normal human cell? [closed]

A diploid human cell has 46 chromosomes. A haploid cell has DNA approximately 3.2 billion bases long. What is the molar concentration of DNA in the cell then? How would we calculate?
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What is the difference between tissue engineering and 3D bioprinting?

3D bioprinting is the modified version of the tissue engineering but when we want to describe the both terms, what are the basic criteria or points to make difference between these?
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Gel electrophoresis bands

Is there any way to calculate the number of bands formed due to sodium dodecyl sulphate-polyacrylamide gel electrophoresis for a particular compound of group of proteins? For example, how could I ...
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318 views

Why does DNA need to be pure for restriction enzymes to act on it?

My textbook states: “In order to cut the DNA with restriction enzymes, it needs to be in pure form, free from other macromolecules. Therefore DNA isolated from other chemicals of the protoplasm like ...
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121 views

Mathematical Biology Jobs/Prospects? [closed]

I'm a junior math major starting to take his first courses in Algebra, Analysis, Topology, Nonlinear and Partial Differential Equations, etc. I am just considering my career options at this point ...
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Which ICs can I use to amplify electrical signals from plants?

I wanted to record variation potential and action potential generated in plants due to some stimulus. So which are the ICs that I can use for the amplifying the signals and what kind of experimental ...
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1answer
311 views

Estimated cost for diy biohacking [closed]

How much would it cost (more precise the better) if i wish to start a diy bio lab in my own house. Assuming that all I want is a functional set and to create maybe simple things like glowing bacteria/...
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141 views

What biotechnology can be used to treat lazy eye, and possibly cure lazy eye? [closed]

I tried very hard researching and finding the root cause of amblyopia. It is mainly because of imbalance in eye muscle. But, there are a lot more complicated causes of this disease. Thus, I need some ...