Questions tagged [biotechnology]

Biotechnology is a field of biology which utilizes biological systems like microorganisms to produce valuable products, such as biofuels, drugs, and vaccines.

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31 views

Finding out the best concentration for my plant extract to be used as drug for diabetes

I am using plant extract of Ajuga parviflora and found out that it possess anti-diabetic properties by using alpha-amylase inhibitory assay. I used various concentration 250 µg/ml(29% inhibiton of ...
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Why haven't humans been able to make blood producing vats?

There are people in hospitals in life threatening circumstances that need blood packages, and they fully depend on blood donors' blood. With our current technology, we have been able to "persuade" ...
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Cell lineage tracing, commercial products

Is anybody familiar with any companies that have commercialized the ability to trace cell lineages? In line with the following methodologies: https://news.harvard.edu/gazette/story/2018/08/genetic-...
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How DNA probe binds

I am studying about southern hybridization now and I've a doubt.After the DNA has been fragmented using restriction enzymes and obtained on nitrocellulose blot , it is still double stranded (the ...
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30 views

Can Cas13 be used with multiple crRNAs in the same reaction?

CRISPR-Cas13 equipped with crRNA (complementary to transcripts of interest) can be designed to target ssRNA transcripts in cells. Upon successful crRNA and ssRNA binding, a fluorescent domain on ...
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How to increase the shelf life of yogurt without refrigeration?

When we make yogurt at home and do not refrigerate it, it will become sour because of conversion of lactose into lactic acid by Lactobacillus bacteria, but this does not happen in case of Nestle's ...
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Why are there two abrupt changes in the genome sequencing price curve?

In the price curve of the genome, from NIH: NIH explained the abrupt drop at 2008 thusly: beginning in January 2008... the sequencing centers transitioned from Sanger-based (dideoxy chain ...
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31 views

What is the use of having a surrogate mother in animal cloning?

In animal cloning, an ovum is collected by an animal and processed to form an embryo after fusion, which is going to be inserted to a surrogate mother. So my question is that why can't the embryo be ...
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23 views

Problem with joining the gene of interest into the open plasmid when their lengths are not the same

Before the recombinant plasmid is obtained in recombinant DNA, the gene of interest is condensed with open plasmid by DNA ligase. So what if the length of the gene of interest is not the same to that ...
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59 views

What is the role of CRISPR-dCas9 in gRNA-dCas9 transcription regulator complexes?

In this paper1, I read that mutant versions of Cas proteins such as a deactivated Cas9 (dCas9) are used alongside a guide-RNA (gRNA) to form variants of CRISPR tool that can function as transcription ...
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How can parentage test be accurately done even though crossing over occurs?

As crossing over occurs when gametes are secreted, so when a zygote is formed after fertilization, the base pairs of DNA in the child should be different from that of the parents. So when gel ...
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Using nanodrop for analysing biological samples other than nucleotides

I am a 3rd timer postdoctoral fellow with some experience in molecular biology and biochemistry, but major skills in Zoology and Natural History. I am studying some natural extracts, and isolating ...
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A plant that is used as antibiotic for trichomycosis axillaris bacterial plates

I want to know a plant may be known as medicinal to treat as an antibiotic for the trichomycosis axillaris bacterial plates
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Why does the material of the culture dish matter in animal tissue culture?

As we know that most animal cells are anchorage dependent cells, so they form a monolayer only when a suitable substrate is provided to them.. When a semi solid nutrient media is prepared and cells ...
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40 views

Transformation of a multicellular organism

I was reading about using RNA-interference as a method of introducing pest-resistance in plants. In tobacco plants, using Agrobacterium vectors, genes specific to the nematode Meloidegyne incognitia ...
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Primer design: Calculating free energy for secondary structure

I'm attempting to automate primer design (specifically, primers for RPA, which requires longer primers [~30-35 nc] than PCR), and need to figure out how to calculate the free energy of a primer pair ...
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2answers
115 views

Is it possible in modern biology to make any person's identical twin (or genetical clone) using his DNA?

I was told by a Biotech professor (who came in our college for a guest lecture) that Bio Technology has now become so advanced that if we want to make an identical twin (or Genetical DNA Twin) of a ...
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72 views

Protein electrophoresis

Three proteins A, B and C of equal molecular weight are being investigated in a study. They contain six, four and four cysteine residues respectively. Only Proteins A and B were treated with β-...
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357 views

Will genetically modified food affect our health?

It's a popular public sentiment that - GM foods like tomatoes (flavr savr) will affect our health.. Is there any logical scientific explanation behind this?
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How do biologists estimate that there is 8% of protein coding genes in human genome?

In class, the professor said that there is about 8% of protein-coding genes in human. I wonder how biologists estimate this without annotating all of human genes. Thank you!
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98 views

What promoter is required for expression of a human protein Y in bacteria?

This is the actual question. It's from an entrance test for a research institute: A scientist wants to express human protein Y in bacteria. For effective expression of this protein he should use (A) ...
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230 views

Why is PCR possible to amplify DNA template before Sanger Sequencing for unknown DNA sequences?

Most of the informations show that both PCR and cloning can be used before Sanger Sequencing to amplify the DNA template. But how is it possible for us to design the primers if we don't know the DNA ...
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112 views

What is the easiest way to measure a human body surface area? [closed]

Looking for accuracy, cost-efficiency, and speed.
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1answer
21 views

I haven't seen any gel lanes

I haven't seen any gel lanes in neither my PCR samples nor the marker itself when photographing them. This is not my first time performing 1.2% agarose gel electrophoresis. I see gel lanes when I ...
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48 views

What are the meaning the nomenclature in high cell density cultivation for the production of a protein? [closed]

I'd like to know the meaning of these terms in a HCDC studies of a modified strain. Max specific yield (mg/g DCW) Max volumetric product yield (g/l) Specific product formation rate Qp (mg/g DCW/h)
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32 views

What does ensemble-based model of enzyme mean?

I am reading Pan et al. (2000), a paper about dihydrofolate reductase (DHFR). They claim using a ensemble-based model of DHFR. What is a ensemble-based model?
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A doubt in biotech question

Which type of gene does not occur naturally in living organisms? Bt gene Endogenous cytoplasmic defense gene The answer is 2. I can't find out what this gene is! Does it mean the gene for ...
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86 views

Selection of recombinant host by color selection method

In recombinant DNA tech, To select a recombinant host from non-recombinants, we have different types of techniques available. The one I'm talking about is the color selection method with the use of ...
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What medium is the bacteria used to synthesize B12 sourced from?

Would that be from water, soil, plants, funguses,...? I asked the question in Quora but that, at the time of posting in this forum, haven't been very helpful. I also have searched for the ...
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What is the difference between Leloir and Non-Leloir glucosyl transferases?

The following questions were on the slides for my biotech course and I haven't been able to find any information on what the answer is. What is the difference between Leloir and Non-Leloir glucosyl ...
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2k views

What would be the molar concentration of human DNA in a normal human cell? [closed]

A diploid human cell has 46 chromosomes. A haploid cell has DNA approximately 3.2 billion bases long. What is the molar concentration of DNA in the cell then? How would we calculate?
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195 views

What is the difference between tissue engineering and 3D bioprinting?

3D bioprinting is the modified version of the tissue engineering but when we want to describe the both terms, what are the basic criteria or points to make difference between these?
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What are challenges faced by algae bioreactors?why they are not deployed on large scale if effective?

I was going through and studying how algae bioreactors work. I am mostly working for closed systems like tubular photobioreactors. I want to create bioreactors to combat pollution problems. However, I ...
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What is the purpose of measuring both VS (volatile solid) and TS (total solid)?

In the biogas process you often measure Volatile solid and Total solids. Can any one explain these concepts more accurately and why it is interesting to look at these properties. The biogas process ...
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117 views

Gel electrophoresis bands

Is there any way to calculate the number of bands formed due to sodium dodecyl sulphate-polyacrylamide gel electrophoresis for a particular compound of group of proteins? For example, how could I ...
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242 views

Why does DNA need to be pure for restriction enzymes to act on it?

My textbook states: “In order to cut the DNA with restriction enzymes, it needs to be in pure form, free from other macromolecules. Therefore DNA isolated from other chemicals of the protoplasm like ...
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1answer
103 views

Mathematical Biology Jobs/Prospects? [closed]

I'm a junior math major starting to take his first courses in Algebra, Analysis, Topology, Nonlinear and Partial Differential Equations, etc. I am just considering my career options at this point ...
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Which ICs can I use to amplify electrical signals from plants?

I wanted to record variation potential and action potential generated in plants due to some stimulus. So which are the ICs that I can use for the amplifying the signals and what kind of experimental ...
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1answer
265 views

Estimated cost for diy biohacking [closed]

How much would it cost (more precise the better) if i wish to start a diy bio lab in my own house. Assuming that all I want is a functional set and to create maybe simple things like glowing bacteria/...
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1answer
4k views

Was HindII the first restriction endonuclease to be extracted?

Background: Guided by wikipedia and pmc I found this paper by Hamilton O. Smith. and .Daniel Nathans. A restriction enzyme is a component of a restriction-modification ...
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132 views

What biotechnology can be used to treat lazy eye, and possibly cure lazy eye? [closed]

I tried very hard researching and finding the root cause of amblyopia. It is mainly because of imbalance in eye muscle. But, there are a lot more complicated causes of this disease. Thus, I need some ...
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1answer
315 views

What step of the cloning process requires an egg cell? Have alternatives been attempted?

In the famous Dolly the sheep an egg cell was required. Why was specifically an egg cell required compared to say sperm cells or any other cell types? Given that egg cells are not trivially harvested ...
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2answers
48 views

How can I describe running a solution four times through a chromatography unit (in an instruction)?

I'm translating a document from Russian: The mean retention time for the first four injections of RNase should be (40.8± 2) min. If the mean RT is beyond this range, adjust the gradient system (...
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504 views

What is synaptic bias?

In non linear model of a neuron there was a mention about bias (Bₖ) which was the summation of the synaptic weights. I want to understand what synaptic bias is and their application/use in neuronal ...
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1answer
588 views

Mass production of bacteria

Is there a way to mass produce a certain species of bacteria? Answers I am looking for should not involve a Petri dish: I am only interested in protocols involving, perhaps, more efficient mediums to ...
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1answer
416 views

Can I do an MS in biotechnology after a bachelors degree in chemical engineering?

I recently graduated from high school and now have to make a decision about my undergrad major. I am interested in biotechnology but my parents suggested that I should opt for a broad based degree and ...
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1answer
447 views

Fermentation of bananas [closed]

I may have access to huge quantities of freshly picked bananas. Searching the web, I have found that the sugar content of the average banana is 14g of fructose (could stand to be corrected on this) ...
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3answers
582 views

Why is a gene for a fluorescent protein used rather than one for an enzyme that produces fluorescent molecule?

Green fluorescent protein (GFP) is a small protein that emits bright green fluorescence in blue light. It was first isolated from the jellyfish. The gene coding for GFP can be expressed in bacteria, ...
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Is PCR a DNA cloning technique?

According to Genomes PCR is A technique that results in exponential amplification of a selected region of a DNA molecule [in test tube]. DNA cloning is Insertion of a fragment of DNA into a ...
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4k views

Role of calcium chloride during competent cell preparation

I am aware of the fact that $CaCl_2$ settles down on the cell wall making it less negative may be by forming bond with Teichoic acid. Also due to the positive charge it attracts DNA (DNA is negatively ...