Questions tagged [biotechnology]

Biotechnology is a field of biology which utilizes biological systems like microorganisms to produce valuable products, such as biofuels, drugs, and vaccines.

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Can Cas13 be used with multiple crRNAs in the same reaction?

CRISPR-Cas13 equipped with crRNA (complementary to transcripts of interest) can be designed to target ssRNA transcripts in cells. Upon successful crRNA and ssRNA binding, a fluorescent domain on ...
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I haven't seen any gel lanes

I haven't seen any gel lanes in neither my PCR samples nor the marker itself when photographing them. This is not my first time performing 1.2% agarose gel electrophoresis. I see gel lanes when I ...
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Gel electrophoresis bands

Is there any way to calculate the number of bands formed due to sodium dodecyl sulphate-polyacrylamide gel electrophoresis for a particular compound of group of proteins? For example, how could I ...
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RNA interference and the former Petunias experiment >

I'm having problems trying to understand how RNA interference works. From what I understood, in simple terms there is this dsRNA which is fragmented into siRNA by the enzyme called Dicer, and those ...
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Can I purify polyhydroxyalkanoates by heating the cells extensively?

Traditional methods of purifying polyhydroxyalkanoates (PHAs) and other bioplastics made by bacteria involve washing the cells with harsh chemicals or strong bases.I'm interested in maintaining the ...
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What is the difference between Endoproteinase Lys-C and Lysyl endopeptidase?

What is the difference between Endoproteinase Lys-C and Lysyl endopeptidase? In the text I'm translating about peptide mapping, "lysine protease" is mentioned (in Russian: лизиновая протеаза). This ...
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Could trees be engineered to produce rainfall-nucleators?

Scientists are using genetic engineering to make trees produce more terpenes. Is it possible to genetically engineer trees to produce more hygroscopic volatile organic compounds? These trees could be ...
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167 views

Why does bacillus thuringiensis produce bt toxin?

Background : B.thuringiensis produces an inactive crystalline toxin during sporulation which when ingested by an insect, gets activated and causes pore formation in gut , subsequently leading to death ...
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Is it possible to intentionally limit bacteria's lifespan?

Say I have a species of bacteria. I want this species to only persist for a set number of generations, after which it simply stops reproducing. Is it possible to do this at all (with known genetic ...
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How to keep activated sludge viable for a week?

I am working in biological waste water treatment and I need to collect some activated sludge samples and have them characterized through different methods. The characterization will take from a few ...
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195 views

What is RNA Engineering

I've been hearing the term passed around, and my own research has led me to some very high-level papers which propose new tools for accomplishing RNA engineering. From the abstract of one of the ...
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PCR limits of detection calculate method

Is any standard method for use to gain PCR sensivity or limit of detection? my mean is not for RT-PCR or Real-Time or any other kind and just simple PCR. After making PCR product free of dNTP and ...
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Kegg in Cytoscape: how to get plots with reaction IDs in place of EC numbers using KeggScape?

I found a KeggScape manual page representing Kegg reactions in Reaction ID format, but I couldn't find how to get a Kegg plot with reaction IDs in place of EC numbers. Does anyone know?
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Can aptamers be used to identify the terminal amino acid residue of a peptide?

Suppose there were DNA or RNA aptamers that bind specifically to a certain terminal amino acid residue. Let's name it aaSA (amino acid specific aptamer). For example, the aptamer binding to terminal ...
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283 views

Why is there a size limit on inserts that a plasmid can accept?

Throughout my undergraduate education I have been taught that plasmids can't carry very large inserts, but I have never been told why. Any insight would be greatly appreciated.
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What should be the distance between plant promoter and gene?

I am trying to clone a rice gene under a different endogenous rice promoter.I will be cloning the CDS of the rice gene.So I wanted to know what is the minimum or maximum distance I should put between ...
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395 views

What specifically allows alpha-complementation in beta-galactosidase?

I'm familiar with the use of alpha-complementation of beta-galactosidase with the pUC alpha-peptide and the M15 lacZ gene product, and would like to hypothetically apply alpha-complementation in other ...
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What medium is the bacteria used to synthesize B12 sourced from?

Would that be from water, soil, plants, funguses,...? I asked the question in Quora but that, at the time of posting in this forum, haven't been very helpful. I also have searched for the ...
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What is the difference between Leloir and Non-Leloir glucosyl transferases?

The following questions were on the slides for my biotech course and I haven't been able to find any information on what the answer is. What is the difference between Leloir and Non-Leloir glucosyl ...
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What are challenges faced by algae bioreactors?why they are not deployed on large scale if effective?

I was going through and studying how algae bioreactors work. I am mostly working for closed systems like tubular photobioreactors. I want to create bioreactors to combat pollution problems. However, I ...
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Which ICs can I use to amplify electrical signals from plants?

I wanted to record variation potential and action potential generated in plants due to some stimulus. So which are the ICs that I can use for the amplifying the signals and what kind of experimental ...
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314 views

Could a 3D Printed Plant Seed be Possible with Existing Technology?

The first genetically cloned animal was Dolly the sheep. As I understand it, this was possible because all that was needed was an embryo containing the cloned genetic material and a womb for that ...
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A Designer Virus

An article at Wikipedia describes color changes that viruses have produced in tulips (without any human intention). And another article (elsewhere on the web) describes viruses that were designed to ...
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Is it possible to separate the binding and catalysis of an enzyme in two steps?

Is it possible to do the following: Enzyme E binds to its substrate S without catalysis; Add a controllable stimulus, such as light, adding or removing chemicals; The enzymatic reaction is triggered ...
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Nutritious protein substance for vitamin enhanced crop?

I am not a bio or science major, but we have a subject, like an elective on biotechnology and we were tasked to think of a product that hasn't been invented yet. My groupmates and I thought of a ...
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Superpatients for Cancer resistance

I was reading an article on MIT Technology review about superpatients for low cholesterol that got me thinking whether such patients exist for cancer. The article is http://www.technologyreview.com/...
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Would biotechnology in agriculture actually decrease biodiversity, as many people say? Does it have to?

I've heard the claim that because farmers would be using the same seed, you would end up with an entire crop population that could be susceptible to a disease. Like if you had a diverse population of ...
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Phage base Biosensor

What kind of biosensor is made by Phages? I need a list of published paper that used Phages for make LCR(inductance (L), capacitance (C), and resistance (R) of a component) biosensor for detection. ...
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Visualizing GFP in transgenic rice seeds?

I want to express GFP in rice as a method for differentiating transgenic from non transgenic rice seeds. I want to know if I can visualize them using a hand held UV lamp as the GFP I will be using has ...
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Which kit provides the highest yield and purity of extracted DNA from FFPE tissue samples?

I'm looking to extract DNA for sequencing from FFPE (Formalin fixed paraffin-embedded) tissue samples. I'd like to use a commercial kit that provides a high yield of good quality DNA.
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Is a neurostimulation implant for the soft-palate component of obstructive sleep apnea plausible?

There are currently ongoing clinical trials for neurostimulation implants designed to keep the tongue from blocking the airway in obstructive sleep apnea. The nerves for this are in the neck and ...
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A plant that is used as antibiotic for trichomycosis axillaris bacterial plates

I want to know a plant may be known as medicinal to treat as an antibiotic for the trichomycosis axillaris bacterial plates
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Why does the material of the culture dish matter in animal tissue culture?

As we know that most animal cells are anchorage dependent cells, so they form a monolayer only when a suitable substrate is provided to them.. When a semi solid nutrient media is prepared and cells ...
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Primer design: Calculating free energy for secondary structure

I'm attempting to automate primer design (specifically, primers for RPA, which requires longer primers [~30-35 nc] than PCR), and need to figure out how to calculate the free energy of a primer pair ...
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Transgenic Plant-Gene source for introduction into host organism

Let's say the plant-gene itself is known, i.e. the exact neucleotide sequence is available in a gene bank (originally sequenced from a tree sample extracted RNA). Previous work has already successfuly ...
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How can site-directed mutagenesis be used to suppress the production of anti-antibodies?

In a previous post of mine, I asked how to supress the creation of anti-antibodies in vivo. In the answer, it was mentioned that site-directed mutagenesis could be used. Currently, I can't find mcuh ...