Questions tagged [calcium]

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Do HEK293T cells express sufficient endogenous G-Proteins for calcium imaging?

I want to transfect different GPCRs to HEK cells and then do calcium imaging. I was wondering if the endogenous expression levels of Gq are high enough.
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46 views

What are “inactive” cells during Ca2+ imaging of neurons?

I am reading this paper, and have found the following Figure (Extended Data Figure 5) where they show maps of active cells in the amygdala as imaged with a miniscope and GCaMP6m: Legend: Using the ...
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Calcium density increment of a neuron

I learned that, as the spike in a neuron occurs, the calcium-ion concentration increases due to opening of the voltage-gated calcium channels. Due to location of these channels, I wonder whether the ...
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2answers
42 views

Turning off the IP3 cascade

After PIP2 has been converted into IP3 and DAG by phospholipase C (PLC), some of the IP3 opens calcium channels in the endoplasmic reticulum and calcium ions can enter the cytoplasm. However, it is ...
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1answer
75 views

Why does hypocalcaemia cause increased muscle contraction?

Calcium is needed for muscle contraction, so how does hypocalcaemia cause increased sustained contraction?
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1answer
37 views

Where do the Ca ions that causes neurotransmitter release from synaptic bouton, come from?

I hope the information you share will help clarify the following doubts and gaps in my knowledge: Where do the Calcium ions in the influx (which then triggers the neurotransmitter vesicles) come from?...
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45 views

What is the role of increased cytosolic calcium concentration after firing, in neuronal cell bodies?

I've come across several studies in which scientists were investigating various questions related to neural activity by focusing on neuronal cell bodies using Calcium imaging. As this article suggests ...
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1answer
84 views

How does coffee cause calcium loss in our body?

Recently on radio a doctor advices to reduce the consumption of coffee as it leads to calcium loss(as my mother described). My initial response was: How could such thing be possible? What kind of ...
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49 views

What causes a tendinopathy to calcify?

I read in this 2012 research paper {1}: The aetiopathogenesis of CT [calcific tendinopathy] is still controversial, but seems to be the result of an active cell-mediated process and a localized ...
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1answer
298 views

How much does calcium reduce iron absorption?

Generally calcium inhibits the absorption of iron, but to what extent? One atom of calcium will not block 100% of iron absorption, that clearly isn't feasible. On the other hand, if someone ate 10 ...
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29 views

Scaling of Ca2+/biochemical signals with dendritic spine size?

I am looking for references that discuss the scaling of biochemical signals in dendritic spines with spine size/synaptic strength. A recent paper (Walker et al. 2017) reports attenuation of NMDA ...
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37 views

Calcium and nitric oxide: does a numerical relationship exist?

Currently, I am trying to understand how nitric oxide and calcium are related during the production of the former (I am not a biologist nor a chemist, so my knowledge about these topics is very poor). ...
2
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1answer
65 views

Calcium concentration in activated dendritic spines?

What are the typical calcium levels reached in single postsynaptic spines following activation of NMDA receptors by an EPSP or backpropagating spike? Everyone seems to refer to that one Neuron paper (...
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Can the calcification response of a marine organisms, at different pH conditions, be assessed by the calcium magnesium ratio?

Can the calcification response of a marine organisms, at different pH conditions, be assessed by the calcium magnesium ratio? the calcium magnesium ratio is a proxy of calcification?
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1answer
52 views

How to induce a steady increase in cyctoplasmic [Ca2+] in HEK293T cells? And is there any simple marker/method to confirm it?

We are trying to find out whether increase or decrease of cytoplasmic calcium concentration affects the interaction of two proteins, using co-IP in HEK293T cells as a readout. In different forums and ...
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1answer
202 views

Has anyone modelled calcium oscillations using Matlab software? [closed]

I have been trying to model calcium oscillations using MatLab, but I cannot obtain the oscillation. I created a function where I put all the equations and used ode15s to call the function. I have ...
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2k views

Effect of extremely hard water on marine life?

When researching water quality standards, I found that a minimum and maximum was set for water hardness. Several sources also state that water can in fact be too hard. The question is how does hard ...
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1answer
267 views

Which hormones/factors affect Calcium absorption in the small intestine and its regulation?

I think the question is very broad and cannot find precise mechanisms. I am not sure about the precise mechanisms of the regulation. I think they are at least Vitamin D, diffusion and specific ...