Questions tagged [cell-culture]
Cell culture is the process of growing and maintaining living cells in vitro.
192
questions
0
votes
0
answers
15
views
What is the solubility of rotenone in ethanol?
I’m trying to kill SH-SY5Y cells with rotenone, but the rotenone does not kill. I saw an article where rotenone is soluble at 5mM in ethanol. I dissolved 1.7 mg in 1 ml of ethanol to obtain a 1ml of ...
0
votes
2
answers
51
views
Is there a way to relate the OD to cells/mL without doing a standard growth curve?
I am helping out with an experiment with multiple bacterial cultures (>20 species). I need to a very specific concentration (cells/mL or CFUs*/mL) for this purpose. Unfortunately it appears that ...
- 176
-2
votes
1
answer
46
views
Does centrifuging the bacteria culture removes bacteria leftovers?
Usually, we centrifuge bacteria culture to either replace the media, control its density (OD), ..etc. I know that when centrifuging the cells are pushed to the bottom while the media (solution) ...
- 23
0
votes
0
answers
23
views
yeast culture live cell extraction
I have been extracting samples for a gene expression assay from yeast. I would like to know how to ensure that the samples I am collecting at 48 hr. culture time, by then culture is probably saturated....
0
votes
0
answers
374
views
What is the difference between BSA fraction V and the initial fraction?
I am preparing a supplemented culture medium and I need to use BSA. There is fraction V of BSA that we normally use for immunocytochemistry and there is another type named the initial fraction that is ...
0
votes
0
answers
53
views
Fixing the cell with 4%PFA and staining with PI to observe cell cycle?
I am wondering whether we can replace 70% ethanol to fix and perm the cells with 4% PFA to observe the cell cycle. Do I understand correctly that 4% PFA can permeate the cells, thus the PI will ...
- 11
0
votes
0
answers
461
views
Virus culture in artificial/synthetic medium
I am looking for a publication/paper in a well a circulated magazine/journal/government study on growing virus culture in synthetic or artificial medium. I have found this link on SARS Cov 2 being ...
- 1
-1
votes
1
answer
78
views
What is the meaning of harboring cryptic in below sentence?
Tissue culture became more widely used at this time because of the introduction of antibiotics, which facilitated long-term cell line propagation, although many people were already warning against ...
- 73
1
vote
1
answer
95
views
Appropriate cell lines to study depression
Short introduction
I'm studying depression from a biochemical point of view. My interest lies in the study of protein biomarkers and I was wondering which cell lines may be appropriate for this ...
- 111
1
vote
0
answers
24
views
How do I cover Cellstrainers?
I‘m doing some experiments for my bachelors thesis and I‘m using some Cell strainers by Roth for it (https://www.carlroth.com/com/en/accessories/cell-strainer-easystrainer™/p/cly9.1). My problem is ...
1
vote
1
answer
82
views
Sabouraud dextrose agar breaking?
I recently started growing some Geotrichum Candidum (GC) on Sabouraud dextrose agar (SDA) beds. The SDA was poured into sterilized petri dishes. After setting the plates in a cooler (20 C) for a day, ...
- 183
0
votes
1
answer
100
views
How to create long term stock for Bacillus subtilis?
I am trying to create a long-term stock for Bacillus subtilis (Bacterial glycerol stocks). I have looked online for guides and step-by-step tutorials and I found that they don't say where the sample ...
5
votes
0
answers
42
views
dilutions in bacterial culture
In overnight bacterial culture, why do we put bacteria in a small flask to culture before moving it to a larger flask? Why not just put the sample in a large flask to begin with? This is in reference ...
- 51
2
votes
1
answer
95
views
Decreasing signals in assay measurements
I'm working with a calcium assay to study the effects of different virulence factors. The assay works, but from day to day the signals of cell lysis go down. Unfortunately, I haven't found an ...
- 401
0
votes
0
answers
40
views
CML (Chronic Myeloid Leukemia) and blast cell percentage
I'm trying to understand the oncogenesis of CML. I have a question about CFC cells and blast cells. Are those the same? I know that in the chronic phase of CML there is a blast percentage of 1-10% of ...
0
votes
0
answers
38
views
QA/monitoring in recombinant vaccine manufacturing
On a16z[0] podcast on vaccines[1], an interesting tidbit came up: vaccine manufacturing in cell cultures is expected to hit a major challenge in terms of quality control due to unpredictable behaviour ...
4
votes
2
answers
679
views
Why does vacuum sealing of foods prevent spoilage from anaerobic bacteria?
I have noted that many purchased food items in containers only require refrigeration after they have been opened, thus eliminating the vacuum seal. Air contains bacteria and fungus spores that enter ...
- 193
0
votes
1
answer
55
views
types of cells in a pharyngeal specimen
I would like to ask what type of cells should someone expect in a pharyngeal smear, in a person, who is infected with a viral infection. Thank you a lot!
- 75
2
votes
1
answer
46
views
Would BMP7 introduced to a Petri dish with cartilage cause chondrocytes to replicate?
Chondrocytes are the cells that make up cartilage. As I understand it, the best growth factor that signals replication of chondrocytes is Bone morphogenetic protein 7.
If supplied with a sufficient ...
0
votes
0
answers
80
views
Public fermentation/cell cultivation database?
I am wondering if there are any large collections of fermentation or cell cultivation data publicly available.
I am interested in all cell types used within industrial biotech and biopharma, ...
- 103
0
votes
1
answer
93
views
how to culture mc38 cells
I work at a start up company in a vivarium. My boss would like me to start working with MC38 Cells. I bought all of the equipment (recommended by a colleague) and should be ready for the cells. Could ...
- 1
1
vote
0
answers
10
views
Cell image database for citotoxicity test
Is there any cell image database for citotoxicity tests? I know that there are several image databases available (like this, this and this one), but none of them have the images for citotoxicity tests....
- 111
1
vote
1
answer
39
views
Image Dataset for Cardiac cells for Mus musculus?
I'm working on developing a transfer learning model to detect cells in a culture using microscopic images.
for that i require data set of images having microscopic images of various cell types. Is ...
0
votes
1
answer
358
views
Why do coliform bacteria smell so bad?
I have done multiple cultures of E. coli bacteria and related species over the course of my education. Almost every time, they had this revolting smell. However, other organisms used for genetics ...
- 231
0
votes
1
answer
160
views
Is it possible to cultivate cheek epithelial cells in a non-CO2 incubator?
CO2 is important for maintaining constant pH in cell culture. Unfortunately, I do not have access to such incubator and buying a new one is expensive. I found a cheap incubator online, but it doesn't ...
1
vote
1
answer
580
views
Is it safe to work with HeLa cells?
Hela cells are infected with HPV. So is it safe to work with them? What are the safety precautions?
- 11
4
votes
1
answer
95
views
Selection marker for a cytoplasmic vector that works at low expression levels?
I need to express a selection marker on a plasmid in human cells (e.g. HEK293). However, I am using a cytoplasmic vector derived from a non-human virus with its own mechanism for transcriptional ...
- 1,883
0
votes
0
answers
32
views
How can I propagate a community of anaerobic bacteria sampled from mud in a bog?
I'm doing research on anaerobic bacteria. In the fall I took mud samples from a bog and have been working with them but I didn't take enough. Now winter has rolled around (Im in Canada) and all the ...
0
votes
0
answers
70
views
Can microbes become "overweight"?
Just out of some weird thoughts, can a microbe (any single-celled organisms, accepting answers for both prokaryotes and eukaryotes) ingest too much food (e.g. via absorbing too much involuntarily ...
- 344
1
vote
1
answer
40
views
Can primary tumor cells grow in 2D culture? [closed]
Is it possible to expand primary tumor cells in 2D culture? Are they adherent cells? Do you have any experience especially with culturing non-small cell lung cancer in 2D?
Thank you.
- 85
0
votes
0
answers
34
views
What is the most durable electronic pipette?
We have a routine task of pipetting substance concentration series in triplicate on cell cultures under a laminar hood. To speed up the process, we use an electronic pipette in the repetitive ...
1
vote
0
answers
18
views
Are controls needed for urine cultures?
If testing for bacteria (gram-negative) in a urine culture, are negative or positive cultures needed?
I could not find anything that says controls are needed unless results seem to be contaminated. ...
- 23
1
vote
0
answers
48
views
Which culture media is suitable for cheek epithelial cells?
A month ago, I tried isolating cheek cells from saliva and the experiment was a success, however, I was wondering if it is possible to cultivate these cells in vitro. I'd say it's possible, but I wasn'...
5
votes
1
answer
133
views
A biological system to measure time
First of all I am a physicist, so I apologise for my non-existent understanding of basic biology. However, I have what I think is an interesting question and this seems like a good place to ask it...
...
- 51
0
votes
1
answer
56
views
"Spontaneous Immortalization"
HaCaT keratinocytes are described as "spontaneously immortalized "(see paper below) due to aneuploidy or chromosomal alteration.
From the paper I get that this is a literal term, that the cells just ...
- 115
2
votes
0
answers
61
views
Pharmacokinetics and cell-membrane permeability of Adenosine triphosphate disodium hydrate
【My Question】
(1) Please tell me the pharmacokinetics of this ATP (or Adenosine triphosphate disodium hydrate) when it is administered orally or intravenously. In particular, Does this material has ...
- 237
4
votes
1
answer
409
views
Why is glycerol supplied/used as 87% solution?
While preparing glycerol stocks of my transformed bacterial cells (DH5alpha) the protocol I had to follow was that I would make one 50% glycerol stock (500µl culture, 500µl glycerol 50%) and another ...
- 81
1
vote
1
answer
108
views
Why do many cells together survive better than one cell alone?
I know that one cell with no ECM is subject to anoikis. That's why in general one cell alone not in its environment is dying.
But are there advantages for a cell to live close to other cells even of ...
- 133
1
vote
2
answers
2k
views
Is my cell-culture contaminated with mold?
I am growing cell cultures at the moment, and I sometimes find 1-2 of these elongated, black structures in one of my 8 wells. Should I be concerned?
I have been growing these for about 5 days at ...
- 11
0
votes
0
answers
29
views
Why don't materials and methods describe culture media in volume?
After reading several materials and methods sections of cell culture experiments, I have not seen the recipe for culture media ever described in terms of volume.
Human adipose tissue was obtained ...
- 121
1
vote
0
answers
56
views
What culturing process / experiment needs a "grid" petri dish (one with a vertical division)
You can buy petri dishes with separations in them like this:
I have studied biology and biochemistry and never come across one of these before. What cell culturing / experiment would this be used in?...
- 165
1
vote
1
answer
67
views
Approximate Cell Doubling Time?
If I know it takes 12 min to occur in mitotic fibroblast cells and I count there are 32 dividing cells in the microscope slide out of a total population of 32034 cells, how do I calculate the ...
- 520
1
vote
0
answers
41
views
Learning industry skills for virology?
I am trying to learn more about virology for a tentative job I could be taking soon, as a quality control person in virology.
However, I have not taken a formal virology course, nor any course work ...
- 1,279
1
vote
0
answers
26
views
Beyond anabolism, what are factors that restrict growth rate?
Beyond obvious constraints in the case of nutrient limitation, what can explain the relatively slow growth rate of cells in rich media?
Lab strains of E. coli grow really, really fast. The cell ...
- 1,883
1
vote
0
answers
24
views
How to take precise/low number of cells from cell lysate? [closed]
I would like to know that how can we take low or precise number of cells from a cell lysate? (without using any protein quantification assay).
Let say,
If I have a cell line having cell density 5x10^...
- 11
0
votes
1
answer
134
views
Continuous (yeast) culture system satisfying multiple criteria
There are multiple ways yeast cells can be grown in liquid cultures.
Batch cultures are easy to prepare but cell density, medium composition and physiology change over time
Chemostats allow to ...
- 585
0
votes
1
answer
966
views
Are cryovials really better than regular tubes for cryopreservation?
What is the rationale for using cryovials instead of regular, sterile centrifuge tubes for the storage of mammalian cells?
With a good aseptic technique and without touching the cap directly, there ...
- 1,883
2
votes
1
answer
186
views
What does it mean to use DMSO as a dissolvant in biology experiemnts?
In many biology publications, I saw that dimethyl sulfoxide (DMSO) is used to dissolve drugs. However, the cell-based assays are still performed in water-based medium such as RPMI or PBS.
My ...
- 21
2
votes
0
answers
4k
views
What is the difference between enrichment media and selective media?
Enrichment media
These media are used to suppress commensal bacteria while allowing the pathogen to remain viable and grow.
[Source: Ananthnarayan and Paniker's textbook of Microbiology.]
...
- 338
1
vote
0
answers
18
views
Can you gain valid toxicological data about a detergent in cell cultures?
I just looked at a paper that in one assay tested a variety of chemicals and chemical mixtures against human cell cultures. They exposed HEK 293 cell cultures for 24 hours to the various chemicals.
...
- 9,144