Questions tagged [cell-culture]
Cell culture is the process of growing and maintaining living cells in vitro.
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Can a non-pure culture be used for reliable rapid phenotypic diagnostic tests?
Can a non-pure culture be used for reliable rapid phenotypic diagnostic tests (e.g. catalase, oxidase & gram stain)?
"Slide Test Method
Obtain a pure culture of the organism to be tested. ...
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Induction of IFN-beta in HEK293T
I'm trying to increase expression of a protein we're attempting to study, UBL7, supposedly unregulated by Type I Interferon and particularly IFN-beta.
I've tried treating HEK293T cells (~60% ...
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Where can I find a video of human cells reacting to cold water vs warm?
I have the following lists:
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What are common culture temperatures?
In literature, authors sometimes refer to 37°C as a common culturing temperature. As I understand it, those are temperatures that are conventionally used for incubators in laboratories. For mesophilic ...
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What kind of bubble this？
This is the MARC-145 I cultivated. Recently, many bubbles appeared in the cells. I don’t know why.
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Cell culture contamination
I've been culturing my cells over 2 weeks and just yesterday these things popped up in my flask , I'm not sure if they're contamination or not.uesterday they looked white and today I found one of them ...
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Need help identifying this microbe
Sorry for the bad image, we don't have a camera hooked up to our culture room microscope yet. But Has anyone seen these kind of (what I'm assuming are) contaminating bacteria before? We see these from ...
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Is somatic fusion/hybridization off-limits to DIY-ers? ELSE: how can an infertile plant's characteristics be salvaged to a fertile one?
I was amazed to discover plant tissue culture could be performed relatively easily at home.
I have been wondering, what's the likelihood of a DIY-er being able to setup and learn a crude and basic lab ...
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Why is my spirulina biomass getting destroyed in my new photobioreactor?
I have a 500L flat plate photobioreactor in my backyard. Whenever I add my culture to the PBR, after 3-4 days of growth, the culture becomes transparent and my biomass is wasted.
I add the stock at a ...
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Does decellularization with sodium lauryl sulfate break down enzymes?
I am currently looking into decellularization with sodium lauryl sulfate since it is effective and cheap. My goal is to break down the cells of a plant leaf and release the chloroplasts to be viewed ...
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Endospores contact with surfaces
I am currently studying endospores-forming bacteria. To be specific B.subtilis. I noticed that the formed spores have strong contact "grip" with the surface it is forming on. For example, ...
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Single-cell subcloning of BV-2 cells?
I am trying to knock-out genes in BV-2 cell line. However, the majority of protocols require cell subcloning and expanding. I tried to subcone these cells and grow clones in DMEM+10% heat-inactivated ...
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MTT assay data pre-processing
I was handed MTT assay data, consisting of drug doses and raw OD scores. The drug treatments were performed three separate times, each followed by MTT assay.
I applied some non-linear mixed-level dose-...
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What is the solubility of rotenone in ethanol?
I’m trying to kill SH-SY5Y cells with rotenone, but the rotenone does not kill. I saw an article where rotenone is soluble at 5mM in ethanol. I dissolved 1.7 mg in 1 ml of ethanol to obtain a 1ml of ...
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Is there a way to relate the OD to cells/mL without doing a standard growth curve?
I am helping out with an experiment with multiple bacterial cultures (>20 species). I need to a very specific concentration (cells/mL or CFUs*/mL) for this purpose. Unfortunately it appears that ...
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Does centrifuging the bacteria culture removes bacteria leftovers?
Usually, we centrifuge bacteria culture to either replace the media, control its density (OD), ..etc. I know that when centrifuging the cells are pushed to the bottom while the media (solution) ...
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What is the difference between BSA fraction V and the initial fraction?
I am preparing a supplemented culture medium and I need to use BSA. There is fraction V of BSA that we normally use for immunocytochemistry and there is another type named the initial fraction that is ...
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Fixing the cell with 4%PFA and staining with PI to observe cell cycle?
I am wondering whether we can replace 70% ethanol to fix and perm the cells with 4% PFA to observe the cell cycle. Do I understand correctly that 4% PFA can permeate the cells, thus the PI will ...
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Virus culture in artificial/synthetic medium
I am looking for a publication/paper in a well a circulated magazine/journal/government study on growing virus culture in synthetic or artificial medium. I have found this link on SARS Cov 2 being ...
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What is the meaning of harboring cryptic in below sentence?
Tissue culture became more widely used at this time because of the introduction of antibiotics, which facilitated long-term cell line propagation, although many people were already warning against ...
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Appropriate cell lines to study depression
Short introduction
I'm studying depression from a biochemical point of view. My interest lies in the study of protein biomarkers and I was wondering which cell lines may be appropriate for this ...
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How do I cover Cellstrainers?
I‘m doing some experiments for my bachelors thesis and I‘m using some Cell strainers by Roth for it (https://www.carlroth.com/com/en/accessories/cell-strainer-easystrainer™/p/cly9.1). My problem is ...
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Sabouraud dextrose agar breaking?
I recently started growing some Geotrichum Candidum (GC) on Sabouraud dextrose agar (SDA) beds. The SDA was poured into sterilized petri dishes. After setting the plates in a cooler (20 C) for a day, ...
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How to create long term stock for Bacillus subtilis?
I am trying to create a long-term stock for Bacillus subtilis (Bacterial glycerol stocks). I have looked online for guides and step-by-step tutorials and I found that they don't say where the sample ...
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dilutions in bacterial culture
In overnight bacterial culture, why do we put bacteria in a small flask to culture before moving it to a larger flask? Why not just put the sample in a large flask to begin with? This is in reference ...
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Decreasing signals in assay measurements
I'm working with a calcium assay to study the effects of different virulence factors. The assay works, but from day to day the signals of cell lysis go down. Unfortunately, I haven't found an ...
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CML (Chronic Myeloid Leukemia) and blast cell percentage
I'm trying to understand the oncogenesis of CML. I have a question about CFC cells and blast cells. Are those the same? I know that in the chronic phase of CML there is a blast percentage of 1-10% of ...
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QA/monitoring in recombinant vaccine manufacturing
On a16z[0] podcast on vaccines[1], an interesting tidbit came up: vaccine manufacturing in cell cultures is expected to hit a major challenge in terms of quality control due to unpredictable behaviour ...
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Why does vacuum sealing of foods prevent spoilage from anaerobic bacteria?
I have noted that many purchased food items in containers only require refrigeration after they have been opened, thus eliminating the vacuum seal. Air contains bacteria and fungus spores that enter ...
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types of cells in a pharyngeal specimen
I would like to ask what type of cells should someone expect in a pharyngeal smear, in a person, who is infected with a viral infection. Thank you a lot!
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Would BMP7 introduced to a Petri dish with cartilage cause chondrocytes to replicate?
Chondrocytes are the cells that make up cartilage. As I understand it, the best growth factor that signals replication of chondrocytes is Bone morphogenetic protein 7.
If supplied with a sufficient ...
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Public fermentation/cell cultivation database?
I am wondering if there are any large collections of fermentation or cell cultivation data publicly available.
I am interested in all cell types used within industrial biotech and biopharma, ...
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how to culture mc38 cells
I work at a start up company in a vivarium. My boss would like me to start working with MC38 Cells. I bought all of the equipment (recommended by a colleague) and should be ready for the cells. Could ...
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Cell image database for citotoxicity test
Is there any cell image database for citotoxicity tests? I know that there are several image databases available (like this, this and this one), but none of them have the images for citotoxicity tests....
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Image Dataset for Cardiac cells for Mus musculus?
I'm working on developing a transfer learning model to detect cells in a culture using microscopic images.
for that i require data set of images having microscopic images of various cell types. Is ...
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Why do coliform bacteria smell so bad?
I have done multiple cultures of E. coli bacteria and related species over the course of my education. Almost every time, they had this revolting smell. However, other organisms used for genetics ...
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Is it possible to cultivate cheek epithelial cells in a non-CO2 incubator?
CO2 is important for maintaining constant pH in cell culture. Unfortunately, I do not have access to such incubator and buying a new one is expensive. I found a cheap incubator online, but it doesn't ...
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Is it safe to work with HeLa cells?
Hela cells are infected with HPV. So is it safe to work with them? What are the safety precautions?
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Selection marker for a cytoplasmic vector that works at low expression levels?
I need to express a selection marker on a plasmid in human cells (e.g. HEK293). However, I am using a cytoplasmic vector derived from a non-human virus with its own mechanism for transcriptional ...
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How can I propagate a community of anaerobic bacteria sampled from mud in a bog?
I'm doing research on anaerobic bacteria. In the fall I took mud samples from a bog and have been working with them but I didn't take enough. Now winter has rolled around (Im in Canada) and all the ...
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Can microbes become "overweight"?
Just out of some weird thoughts, can a microbe (any single-celled organisms, accepting answers for both prokaryotes and eukaryotes) ingest too much food (e.g. via absorbing too much involuntarily ...
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Can primary tumor cells grow in 2D culture? [closed]
Is it possible to expand primary tumor cells in 2D culture? Are they adherent cells? Do you have any experience especially with culturing non-small cell lung cancer in 2D?
Thank you.
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What is the most durable electronic pipette?
We have a routine task of pipetting substance concentration series in triplicate on cell cultures under a laminar hood. To speed up the process, we use an electronic pipette in the repetitive ...
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Are controls needed for urine cultures?
If testing for bacteria (gram-negative) in a urine culture, are negative or positive cultures needed?
I could not find anything that says controls are needed unless results seem to be contaminated. ...
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Which culture media is suitable for cheek epithelial cells?
A month ago, I tried isolating cheek cells from saliva and the experiment was a success, however, I was wondering if it is possible to cultivate these cells in vitro. I'd say it's possible, but I wasn'...
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A biological system to measure time
First of all I am a physicist, so I apologise for my non-existent understanding of basic biology. However, I have what I think is an interesting question and this seems like a good place to ask it...
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"Spontaneous Immortalization"
HaCaT keratinocytes are described as "spontaneously immortalized "(see paper below) due to aneuploidy or chromosomal alteration.
From the paper I get that this is a literal term, that the cells just ...
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Pharmacokinetics and cell-membrane permeability of Adenosine triphosphate disodium hydrate
【My Question】
(1) Please tell me the pharmacokinetics of this ATP (or Adenosine triphosphate disodium hydrate) when it is administered orally or intravenously. In particular, Does this material has ...
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Why is glycerol supplied/used as 87% solution?
While preparing glycerol stocks of my transformed bacterial cells (DH5alpha) the protocol I had to follow was that I would make one 50% glycerol stock (500µl culture, 500µl glycerol 50%) and another ...
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Why do many cells together survive better than one cell alone?
I know that one cell with no ECM is subject to anoikis. That's why in general one cell alone not in its environment is dying.
But are there advantages for a cell to live close to other cells even of ...
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