Questions tagged [cell-culture]
Cell culture is the process of growing and maintaining living cells in vitro.
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Did my cellculture develop an immunity against toxins/virulence factors?
at the moment I'm working with an assay which detects cell death by measuring calcium influx.
Unfortunately, the results are not like I wish them to be.
The signals that I measure, decrease from day ...
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1answer
68 views
Decreasing signals in assay measurements
I'm working with a calcium assay to study the effects of different virulence factors. The assay works, but from day to day the signals of cell lysis go down. Unfortunately, I haven't found an ...
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Aerosolised particle released during autoclaving
During autoclave heat-up, the inside chamber's air is released in order to be displaced by steam. Because of that, many modern autoclaves are equipped with filters at the release outlet. The ...
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CML (Chronic Myeloid Leukemia) and blast cell percentage
I'm trying to understand the oncogenesis of CML. I have a question about CFC cells and blast cells. Are those the same? I know that in the chronic phase of CML there is a blast percentage of 1-10% of ...
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QA/monitoring in recombinant vaccine manufacturing
On a16z[0] podcast on vaccines[1], an interesting tidbit came up: vaccine manufacturing in cell cultures is expected to hit a major challenge in terms of quality control due to unpredictable behaviour ...
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Why does vacuum sealing of foods prevent spoilage from anaerobic bacteria?
I have noted that many purchased food items in containers only require refrigeration after they have been opened, thus eliminating the vacuum seal. Air contains bacteria and fungus spores that enter ...
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1answer
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types of cells in a pharyngeal specimen
I would like to ask what type of cells should someone expect in a pharyngeal smear, in a person, who is infected with a viral infection. Thank you a lot!
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1answer
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Would BMP7 introduced to a Petri dish with cartilage cause chondrocytes to replicate?
Chondrocytes are the cells that make up cartilage. As I understand it, the best growth factor that signals replication of chondrocytes is Bone morphogenetic protein 7.
If supplied with a sufficient ...
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36 views
Public fermentation/cell cultivation database?
I am wondering if there are any large collections of fermentation or cell cultivation data publicly available.
I am interested in all cell types used within industrial biotech and biopharma, ...
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1answer
35 views
how to culture mc38 cells
I work at a start up company in a vivarium. My boss would like me to start working with MC38 Cells. I bought all of the equipment (recommended by a colleague) and should be ready for the cells. Could ...
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Cell image database for citotoxicity test
Is there any cell image database for citotoxicity tests? I know that there are several image databases available (like this, this and this one), but none of them have the images for citotoxicity tests....
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1answer
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Image Dataset for Cardiac cells for Mus musculus?
I'm working on developing a transfer learning model to detect cells in a culture using microscopic images.
for that i require data set of images having microscopic images of various cell types. Is ...
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1answer
76 views
Why do coliform bacteria smell so bad?
I have done multiple cultures of E. coli bacteria and related species over the course of my education. Almost every time, they had this revolting smell. However, other organisms used for genetics ...
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1answer
36 views
Is it possible to cultivate cheek epithelial cells in a non-CO2 incubator?
CO2 is important for maintaining constant pH in cell culture. Unfortunately, I do not have access to such incubator and buying a new one is expensive. I found a cheap incubator online, but it doesn't ...
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1answer
260 views
Is it safe to work with HeLa cells?
Hela cells are infected with HPV. So is it safe to work with them? What are the safety precautions?
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1answer
48 views
Selection marker for a cytoplasmic vector that works at low expression levels?
I need to express a selection marker on a plasmid in human cells (e.g. HEK293). However, I am using a cytoplasmic vector derived from a non-human virus with its own mechanism for transcriptional ...
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How can I propagate a community of anaerobic bacteria sampled from mud in a bog?
I'm doing research on anaerobic bacteria. In the fall I took mud samples from a bog and have been working with them but I didn't take enough. Now winter has rolled around (Im in Canada) and all the ...
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Can microbes become “overweight”?
Just out of some weird thoughts, can a microbe (any single-celled organisms, accepting answers for both prokaryotes and eukaryotes) ingest too much food (e.g. via absorbing too much involuntarily ...
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1answer
38 views
Can primary tumor cells grow in 2D culture? [closed]
Is it possible to expand primary tumor cells in 2D culture? Are they adherent cells? Do you have any experience especially with culturing non-small cell lung cancer in 2D?
Thank you.
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What is the most durable electronic pipette?
We have a routine task of pipetting substance concentration series in triplicate on cell cultures under a laminar hood. To speed up the process, we use an electronic pipette in the repetitive ...
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Are controls needed for urine cultures?
If testing for bacteria (gram-negative) in a urine culture, are negative or positive cultures needed?
I could not find anything that says controls are needed unless results seem to be contaminated. ...
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Which culture media is suitable for cheek epithelial cells?
A month ago, I tried isolating cheek cells from saliva and the experiment was a success, however, I was wondering if it is possible to cultivate these cells in vitro. I'd say it's possible, but I wasn'...
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1answer
108 views
A biological system to measure time
First of all I am a physicist, so I apologise for my non-existent understanding of basic biology. However, I have what I think is an interesting question and this seems like a good place to ask it...
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1answer
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“Spontaneous Immortalization”
HaCaT keratinocytes are described as "spontaneously immortalized "(see paper below) due to aneuploidy or chromosomal alteration.
From the paper I get that this is a literal term, that the cells just ...
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Pharmacokinetics and cell-membrane permeability of Adenosine triphosphate disodium hydrate
ćMy Questionć
(1) Please tell me the pharmacokinetics of this ATP (or Adenosine triphosphate disodium hydrate) when it is administered orally or intravenously. In particular, Does this material has ...
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1answer
238 views
Why is glycerol supplied/used as 87% solution?
While preparing glycerol stocks of my transformed bacterial cells (DH5alpha) the protocol I had to follow was that I would make one 50% glycerol stock (500Āµl culture, 500Āµl glycerol 50%) and another ...
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1answer
78 views
Why do many cells together survive better than one cell alone?
I know that one cell with no ECM is subject to anoikis. That's why in general one cell alone not in its environment is dying.
But are there advantages for a cell to live close to other cells even of ...
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2answers
481 views
Is my cell-culture contaminated with mold?
I am growing cell cultures at the moment, and I sometimes find 1-2 of these elongated, black structures in one of my 8 wells. Should I be concerned?
I have been growing these for about 5 days at ...
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Why don't materials and methods describe culture media in volume?
After reading several materials and methods sections of cell culture experiments, I have not seen the recipe for culture media ever described in terms of volume.
Human adipose tissue was obtained ...
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35 views
What culturing process / experiment needs a “grid” petri dish (one with a vertical division)
You can buy petri dishes with separations in them like this:
I have studied biology and biochemistry and never come across one of these before. What cell culturing / experiment would this be used in?...
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1answer
55 views
Approximate Cell Doubling Time?
If I know it takes 12 min to occur in mitotic fibroblast cells and I count there are 32 dividing cells in the microscope slide out of a total population of 32034 cells, how do I calculate the ...
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0answers
29 views
Learning industry skills for virology?
I am trying to learn more about virology for a tentative job I could be taking soon, as a quality control person in virology.
However, I have not taken a formal virology course, nor any course work ...
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0answers
25 views
Beyond anabolism, what are factors that restrict growth rate?
Beyond obvious constraints in the case of nutrient limitation, what can explain the relatively slow growth rate of cells in rich media?
Lab strains of E. coli grow really, really fast. The cell ...
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0answers
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How to take precise/low number of cells from cell lysate? [closed]
I would like to know that how can we take low or precise number of cells from a cell lysate? (without using any protein quantification assay).
Let say,
If I have a cell line having cell density 5x10^...
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1answer
125 views
Continuous (yeast) culture system satisfying multiple criteria
There are multiple ways yeast cells can be grown in liquid cultures.
Batch cultures are easy to prepare but cell density, medium composition and physiology change over time
Chemostats allow to ...
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1answer
417 views
Are cryovials really better than regular tubes for cryopreservation?
What is the rationale for using cryovials instead of regular, sterile centrifuge tubes for the storage of mammalian cells?
With a good aseptic technique and without touching the cap directly, there ...
2
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1answer
104 views
What does it mean to use DMSO as a dissolvant in biology experiemnts?
In many biology publications, I saw that dimethyl sulfoxide (DMSO) is used to dissolve drugs. However, the cell-based assays are still performed in water-based medium such as RPMI or PBS.
My ...
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0answers
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What is the difference between enrichment media and selective media?
Enrichment media
These media are used to suppress commensal bacteria while allowing the pathogen to remain viable and grow.
[Source: Ananthnarayan and Paniker's textbook of Microbiology.]
...
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Can you gain valid toxicological data about a detergent in cell cultures?
I just looked at a paper that in one assay tested a variety of chemicals and chemical mixtures against human cell cultures. They exposed HEK 293 cell cultures for 24 hours to the various chemicals.
...
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Isolation of Intact Granules from Mast Cells
How to isolate intact granules from mast cells without using sucrose and percoll?
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1answer
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What is the recommended culture volume for HeLa cells and seeding number for 384 well plate wells?
For 24 well plate wells, thermofischer recommends to seed 0.05E6 cells with 0.5 to 1 ml of medium. What are recommended numbers for a 384 well plate well?
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1answer
41 views
Growth mediums required for human cell cultures
So i would like to try a culture some of my keratinocytes just to see if i can do it. Ive read about a few different types of growth medium such as DMEM and RPMI but i am not sure about when to use ...
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1answer
109 views
Major effects on cells grown with 5% FBS supplemented media instead of 10% FBS?
I maintain a few cell lines in the laboratory (industrial R&D) that I use regularly for experiments. I realized today that the media i prepared for the week on monday was only supplemented with 50 ...
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What is(are) the mechanism(s) that stop cells from fusing in vivo?
I just learned about the phenomenon of 'cell fusion' in which two diploid somatic cells can combine into some aneuploid cell in vitro and proceed to proliferate in culture. Apparently this can even ...
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Low cost cell free expression system
I am a graduate student looking to incorporate some protein engineering into my training. I have been reading a lot about E coli lysate based systems, but they still require 3rd party additives like ...
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1answer
187 views
Chunks of cells after trypsinization
Recently I've came across a problem after trypsinization of my stably transfected CHO-cells. When I add medium to rinse off the cells from the bottom of the flask, there are usually big chunks of ...
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1answer
62 views
Minimum hardware and knowledge to study effects on live cells [closed]
I'm very interested to study the effects (or reactions) of different forces (magnetic fields, electric fields, etc) on living tissues/cells.
I know I have to have a living cell, the force applied and ...
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1answer
94 views
What are the meaning the nomenclature in high cell density cultivation for the production of a protein? [closed]
I'd like to know the meaning of these terms in a HCDC studies of a modified strain.
Max specific yield (mg/g DCW)
Max volumetric product yield (g/l)
Specific product formation rate Qp (mg/g DCW/h)
6
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1answer
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Microinjection intestinal organoids
I am attempting to microinject small intestinal organoids, using the Eppendorf FemtoJet 4i microinjector and Femtotip capillaries, and have come across several issues. This is a new set up in our lab, ...
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1answer
35 views
Is there a standard way of measuring culture nutrient levels in real time?
I am working on optimizing growth rates for a culture, and one thing I'm trying to monitor is nutrient consumption rates to pinpoint any bottlenecks, i.e. the culture goes through all the calcium in a ...
