Questions tagged [cell-cycle]
The cell-cycle tag has no usage guidance.
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What's the significance of DNA replication (S phase) before Meiosis?
From what I'm able to understand, Meiosis should end up with haploid cells with chromosomes having single chromatids (I don't know why it's necessary, but alright)
I understand that meiosis has a ...
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does retrovirus need to manipulate cell cycle by neutralizing Rb and/or p53?
Early studies of transforming retroviruses led to discovery of oncogenes, while studies of DNA viruses (like AV40) led to discovery of p53 and Rb and tumor suppressor genes. Frank McCormick says in ...
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Interphase is the process in which DNA is duplicated/replicated and is the longest phase [closed]
A test question that I completed as True just wanted to know if I'm right or not:
The Question goes as follows:
Please write True or False for the following question; if False, correct the Bolded ...
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Mechanism for switch of embryonic stem cells from "fast" cell cycle to "normal" cell cycle?
It is well-known that embryonic stem cells have a fast cell cycle (very roughly about 12 hours), while typically normal cells have a much longer cell cycle (very roughly about 24 hours).
Of course, it ...
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How frequent are transposons in action? [closed]
A transposable element (TE, transposon, or jumping gene) is a DNA sequence that can change its position within a genome, sometimes creating or reversing mutations and altering the cell's genetic ...
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Fixing the cell with 4%PFA and staining with PI to observe cell cycle?
I am wondering whether we can replace 70% ethanol to fix and perm the cells with 4% PFA to observe the cell cycle. Do I understand correctly that 4% PFA can permeate the cells, thus the PI will ...
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homologous recombination and non-homologous recombination ratio during S-phase
Can I assume that it is easier to do targeted gene knock-in in rapidly dividing cells because they should have a short period of G1? Is there an easy way to measure the relative amounts of homologous ...
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Why do nearby cells have similar meiosis periods?
A few days ago, I conducted an experiment to observe the meiosis of rye ears. During the experiment, I observed that cells in similar locations have similar meiosis period. Can anybody tell me why ...
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cell proliferation assays for research proposal
I'm an undergrad Biology student and I'm working on a research proposal for a certain course. I need help or guide on cell proliferation assays. I have a target gene , recent papers show that this ...
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What is the minimum-volume : neutral-volume : maximum-volume of the molecules that make up the SARS-Cov-2 virus-fusion machine?
The "neutral-volume" is the over-lapping volume between minimum-volume & maximum-volume of the molecules that make up the SARS-Cov-2-fusion machine. Those identifying potential ...
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Can we see the cell cycle of the cells administered using IVIS-like device?
My question;
Can we measure the cell cycle of the administered cells from outside the body, while the recipient is still alive ? Preferably, by using a device such as IVIS that can read fluorescent ...
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When and Why does G0 phase occur?
I've asked this question, specifically because i've seen this question.
As we see in this diagram , G0 phase occurs after M phase and at a specific point within G1 phase.
Is there a meaning to ...
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quiescent stage (G0) occurs at the end of M phase or G1 phase: [closed]
I know that G0 phase occurs when certain cells exit the cell cycle during G1 phase but that means it goes to G0 phase after M phase right?
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Can mutation take place in G1 and G2 phases during the cell cycle?
We know that the DNA replicates during the S phase in Interphase. There it might undergo a number of mutations. We also know that the forward half strands are more susceptible to undergoing a mutation ...
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Chromosomal structures in S phase and Prophase
Of S Phase:
Existing DNA molecule acts as a template to synthesize new DNA molecule. The cell doubles it's DNA, yet the number of chromosomes remain the same
Of Prophase:
The chromatin ...
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DRAQ5 and PI incompatible?
I'm interested to analyse hemolymph cells from a marine mollusc. I wanna count only "alive" cells so my strategy was the following: DRAQ5+ (nucleated cells) and PI- (alive cells) and identify ...
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Is p53 a cyclin dependent kinase? [closed]
I've been reading some research papers about p53 and associated tumour suppressor proteins, such as p21. I see them referred to and associated with cyclin-dependent kinases. Is p53,p63 et cetera part ...
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Does a cell suspend or exit cell cycle at G0?
In an exam, there was one question which asked whether the cell exits or suspends cell cycle at G0 phase.
I answered that it exits cell cycle but the official answer key says it suspends cell cycle.
...
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Approximate Cell Doubling Time?
If I know it takes 12 min to occur in mitotic fibroblast cells and I count there are 32 dividing cells in the microscope slide out of a total population of 32034 cells, how do I calculate the ...
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When does DNA replicate actually?
I read on Wikipedia that when the cell enters prophase during mitosis , the DNA has already been duplicated , that is the DNA is replicated in the chromatin form , but here I see the picture which ...
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Confusion about the duration of different phases of the cell cycle such as G1, G2 & S
We all know that normally most of our cells (which are alive and have nuclei - except for cells such as "stem cells", "red blood cells", etc) spend most of their lives in ...
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Does meosis takes place in cell cycle?
Cell cycle is as follows- g1,s,g2,m( mitosis). Does this m stands for mitosis only or does this "m" stands for meosis also
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What is the meaning of "gap" in G1 phase?
The full form of G1 phase is Gap one phase. G1 os also called first gap phase. Is there any specific meaning of " gap" here ?
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What is the meaning of "Rb is in the active state"?
I am reading about cyclins, cdks and Rb (Retinoblastoma) and some of the terminology is not clear to me.
I understand that when CDK phosphorylates Rb, it disconnects from E2F, and E2F can act as a ...
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Why don't cells double gene expression after S-Phase?
In the cell cycle (G1-S-G2-M), all of the DNA is replicated during the S or Synthesis stage. The cell may then spend some considerable time in the G2 phase before splitting in the M phase. Since there ...
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Crispr/CAS9 genome editing is actually processed at which phase of Cell cycle?
all,
Is it only happens in certain phases, like S, G1,...or it can happen any time....or maybe, it has some perferences.
Put it in another word, does it going to processe edit if the cell is not ...
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Cell cycle arrest and apoptosis
Is cell cycle arrest needed for apoptosis to occur? I note that some of the factors that mediate cell cycle arrest induction (e.g. DNA damage) happen to be responsible for initiating apoptosis as well....
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What determines the reversibility of cell of G0 phase in cell cycle?
From Weinberg's The Biology of Cancer: A cell that has been recently formed by mitosis must decide whether it will once again initiate a new round of active growth and division or retreat into non-...
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Is agriculture really a net contributor of greenhouse gases?
A lot of scientific studies and credible sources indicate that agriculture is one of the major contributors of greenhouse gases. The exact numbers seem to vary a lot, I've seen everything from 8% to ...
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Flow Cytometry to learn about Cell cycle
I am posting my Flow Cytometry data here again because I realize that there are many people who are willing to help me, and I can learn a lot from you guys.
In this cell culture Lab, we use Cell U-...
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During interphase, is DNA wrapped around histones?
Are histone proteins present around DNA in the nucleus during all of interphase (including G1) or do histone proteins only form later on when chromosomes are condensing into chromatids?
Thanks in ...
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How does the cell cycle affect stem cell fate?
I keep reading about the stages of the cell cycle affecting stem cell fate, however I do not understand how. After all, any cell will have to pass through the whole of the cell cycle, and go through ...
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Advantages and disadvantages of sporulation compared with competence in bacteria?
Why do bacteria have both of these mechanisms to deal with the same environmental stress: nutrient deprivation? In a culture exposed to this condition, often both competent cells and sporulated cells ...
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When does the kinetochore form?
According to Wikipedia,
the outer plate... is assembled in the surface of the chromosomes when the nuclear envelope breaks down.
However is makes not mention of when the inner portion of the ...
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MCQ - Events in the Mitotic cycle
The following question confused me at first
However, I presumed that it must be in comparison to meiosis. In that case D would be correct.
However, the correct answer is A.
How can this be? I know ...
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Is there interphase between meiosis I and meiosis II?
Is there interphase between meiosis I and meiosis II?
After 2 haploid cells are formed in meiosis I, do the cells go through a period of interphase (G1, S, G2) or do they go directly to meiosis II?
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Cell cycle-regulated genes and mRNA
I am a mathematician and my knowledge about biology is close to zero. I am reading a bioinformatics paper and I would like to understand a bit more about the biology task they are talking about. I ...
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In cell division, are daughter cells identical?
I understand that after a cell replicates, there will be two daughter cells instead of one. But wouldn't one of them be the old cell that created the second one?
The old cell having gone through G0, ...
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Do human somatic cells have 46 chromosomes all the time?
As far as I know, a human somatic cell has the cell cycle:
Interphase and Replication (mitosis). In interphase, the cell has 2 growth phases (G1 + G2) and a synthesis one (S phase being in between ...
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Contact Inhibition of Cell Division: Signaling Pathway
The following article refers to contact inhibition of cell division in epithelial cells, specifically MDCK cells: Collective and single cell behavior in epithelial contact inhibition.
In their review ...
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Are both (partial) copies of DNA transcribed in S and G2 phases of cell cycle?
For a little less than half the cell cycle, a significant number of genes are represented twice (just before dividing). Does the cell differentiate between these DNA in any way or are transcription ...
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Alternatives to commercially available flow cytometric analysis software for use in cell cycle analysis?
I know that many labs use either FlowJo or FCS Express to plot and analyze their flow data. These seem like the two standards for most labs (and I have used FlowJo before). However, my current funding ...
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Is the nuclear envelope present in G1 of interphase?
Is the nuclear envelope present in G1 of interphase of eukaryotic cell? If so how does a method like Calcium mediated transfection get DNA past the nuclear envelope?
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Concentration of caffeine in physiologically relevant setting
Caffeine is known to overcome G2/M (and possibly other types of) cell cycle arrest through its effects on DNA repair machinery and is used as a positive control in studies of cell cycle arrest. In ...
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How many centrioles/basal bodies are there in multi-ciliated cells throughout the cell cycle?
I thought there were only two centrioles per cell, that convert to the basal body at some point during the cell cycle. I also thought there's one basal body per cilium, so I'm not clear on where the ...
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