Questions tagged [cell-cycle]
The cell-cycle tag has no usage guidance.
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Can we see the cell cycle of the cells administered using IVIS-like device?
My question;
Can we measure the cell cycle of the administered cells from outside the body, while the recipient is still alive ? Preferably, by using a device such as IVIS that can read fluorescent ...
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Advantages and disadvantages of sporulation compared with competence in bacteria?
Why do bacteria have both of these mechanisms to deal with the same environmental stress: nutrient deprivation? In a culture exposed to this condition, often both competent cells and sporulated cells ...
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homologous recombination and non-homologous recombination ratio during S-phase
Can I assume that it is easier to do targeted gene knock-in in rapidly dividing cells because they should have a short period of G1? Is there an easy way to measure the relative amounts of homologous ...
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Why do nearby cells have similar meiosis periods?
A few days ago, I conducted an experiment to observe the meiosis of rye ears. During the experiment, I observed that cells in similar locations have similar meiosis period. Can anybody tell me why ...
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When and Why does G0 phase occur?
I've asked this question, specifically because i've seen this question.
As we see in this diagram , G0 phase occurs after M phase and at a specific point within G1 phase.
Is there a meaning to ...
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DRAQ5 and PI incompatible?
I'm interested to analyse hemolymph cells from a marine mollusc. I wanna count only "alive" cells so my strategy was the following: DRAQ5+ (nucleated cells) and PI- (alive cells) and identify ...
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Crispr/CAS9 genome editing is actually processed at which phase of Cell cycle?
all,
Is it only happens in certain phases, like S, G1,...or it can happen any time....or maybe, it has some perferences.
Put it in another word, does it going to processe edit if the cell is not ...
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does retrovirus need to manipulate cell cycle by neutralizing Rb and/or p53?
Early studies of transforming retroviruses led to discovery of oncogenes, while studies of DNA viruses (like AV40) led to discovery of p53 and Rb and tumor suppressor genes. Frank McCormick says in ...
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Fixing the cell with 4%PFA and staining with PI to observe cell cycle?
I am wondering whether we can replace 70% ethanol to fix and perm the cells with 4% PFA to observe the cell cycle. Do I understand correctly that 4% PFA can permeate the cells, thus the PI will ...
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cell proliferation assays for research proposal
I'm an undergrad Biology student and I'm working on a research proposal for a certain course. I need help or guide on cell proliferation assays. I have a target gene , recent papers show that this ...
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What is the minimum-volume : neutral-volume : maximum-volume of the molecules that make up the SARS-Cov-2 virus-fusion machine?
The "neutral-volume" is the over-lapping volume between minimum-volume & maximum-volume of the molecules that make up the SARS-Cov-2-fusion machine. Those identifying potential ...
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When does the kinetochore form?
According to Wikipedia,
the outer plate... is assembled in the surface of the chromosomes when the nuclear envelope breaks down.
However is makes not mention of when the inner portion of the ...
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