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Questions tagged [cell-sorting]

Questions relating to the separation of cells on the basis of physical properties.

3
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0answers
78 views

What is this thing found in my saliva?

Found in my saliva (Optical microscope 40x)
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vote
0answers
37 views

Sorting beads with FACS

Does anyone know if it is possible to sort micro-beads, ideally magnetic, using FACS and if so what diameters can be sorted. I was thinking of using fluorescent beads that might help facilitate this ...
1
vote
1answer
587 views

Flow cytometry data Analysis [closed]

everyone. Since I am new in the flow cytometry technique, I am not really sure how to analyze the result. We use cell U-937 Lymphoblast.All Cells Could Anyone help to explain me this one? Thanks ...
2
votes
1answer
2k views

Staining cells for FACS at 4 degrees or ambient temperature

I'm sorting a 293 derived line. One thing that is worrisome for me is cell viability after the sort. Usually I have been staining and washing at room temperature (on the benchtop) on a nutator. I have ...
7
votes
1answer
5k views

Magnetic-activated cell sorting vs. FACS

When sorting cell populations it is possible to use either magnetic-activated cell sorting or fluorescence-activated cell-sorting (FACS). I am wondering when you would choose either technique and what ...
3
votes
1answer
295 views

How can I determine the purity of cells isolated from rat brains if I cannot use FACS, Immunohistochemistry or SEM anaysis?

Please help. I know how to isolate the different cells (astrocytes, other glial cells, neurons and synaptosomes) from brain tissue using a Ficoll-based separation but how do I determine the purity of ...
4
votes
1answer
130 views

Missing cells from immunopanning

Why do I not have any cells left in my positive panning plate after transferring from the negative panning plate during immunopanning? I am trying to purify retinal ganglion cells from postnatal rats ...
5
votes
1answer
7k views

What is immunopanning (vs. immunoprecipitation and FACS)?

I had never heard the term before today. From what I can tell, it's using antibodies to purify a cell population of interest. I would appreciate more details, especially in how it differs from "...