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Questions tagged [cell-sorting]

Questions relating to the separation of cells on the basis of physical properties.

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CD45 as a microglial marker in Fluorescence-Activated Cell Sorting (FACS)

I am reading this article: Microglial cannabinoid receptor type 1 mediates social memory deficits in mice produced by adolescent THC exposure and 16p11.2 duplication. In Figure 1a, the authors are ...
neurosciencecalc's user avatar
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115 views

Is there a DNase that works at low temperature, and is it also commerically available?

In preparation for FACS, I need to keep mammalian neurons on ice (in PBS-- / BSA), but as they still die while waiting ~30 minutes, they release their DNA into the solution. This leads to cell clumps. ...
bud.dugong's user avatar
1 vote
0 answers
10 views

Why am I finding peculiar B cell population when pbmcs are cultured for 3 days with CD40L?

While performing intracellular cytokine assay on B cells, I am finding a peculiar population. I use 2*10^6 cells for studying each functional marker. The pbmcs are cultured for 72 hours, with CD40L ...
SOHAM BHADRA's user avatar
1 vote
2 answers
269 views

How are single cells sorted in an scRNA-Seq protocol by FACS?

I am computational guy trying to understand how FACS sorting works in an scRNA-Seq protocol. About sorting single cells in each well of a 384-well plate, I have a question that would be grateful if ...
MCH's user avatar
  • 141
2 votes
0 answers
329 views

What is this thing found in my saliva?

Found in my saliva (Optical microscope 40x)
Jacopo Gabrielli's user avatar
1 vote
0 answers
123 views

Sorting beads with FACS

Does anyone know if it is possible to sort micro-beads, ideally magnetic, using FACS and if so what diameters can be sorted. I was thinking of using fluorescent beads that might help facilitate this ...
wl284's user avatar
  • 101
1 vote
1 answer
1k views

Flow cytometry data Analysis [closed]

everyone. Since I am new in the flow cytometry technique, I am not really sure how to analyze the result. We use cell U-937 Lymphoblast.All Cells Could Anyone help to explain me this one? Thanks ...
Sokviseth's user avatar
2 votes
1 answer
4k views

Staining cells for FACS at 4 degrees or ambient temperature

I'm sorting a 293 derived line. One thing that is worrisome for me is cell viability after the sort. Usually I have been staining and washing at room temperature (on the benchtop) on a nutator. I have ...
jwillis0720's user avatar
7 votes
1 answer
8k views

Magnetic-activated cell sorting vs. FACS

When sorting cell populations it is possible to use either magnetic-activated cell sorting or fluorescence-activated cell-sorting (FACS). I am wondering when you would choose either technique and what ...
Wolgast's user avatar
  • 747
3 votes
1 answer
307 views

How can I determine the purity of cells isolated from rat brains if I cannot use FACS, Immunohistochemistry or SEM anaysis?

Please help. I know how to isolate the different cells (astrocytes, other glial cells, neurons and synaptosomes) from brain tissue using a Ficoll-based separation but how do I determine the purity of ...
subhash narasimhan's user avatar
4 votes
1 answer
143 views

Missing cells from immunopanning

Why do I not have any cells left in my positive panning plate after transferring from the negative panning plate during immunopanning? I am trying to purify retinal ganglion cells from postnatal rats ...
Rachel's user avatar
  • 41
5 votes
1 answer
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What is immunopanning (vs. immunoprecipitation and FACS)?

I had never heard the term before today. From what I can tell, it's using antibodies to purify a cell population of interest. I would appreciate more details, especially in how it differs from "...
blep's user avatar
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