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Questions tagged [centrifugation]

a process which involves the application of the centrifugal force for the sedimentation of heterogeneous mixtures with a centrifuge, and is used in industrial and laboratory settings. Use this tag in questions related to instruments that exploit this technique, or fields of biology in which this technique is applied.

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Does centrifuging and filtering supernatant confer an advantage compared to just filtering?

We are trying to isolate bacteriophages from environmental soil samples. One of the first steps is elution, centrifugation and filtering of the soil samples. One protocol suggests to: Elute the soil ...
Johan Wikström's user avatar
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Is ribosome denser and smaller than nucleus. Also sone relations in centrifugation related to size and density

I want to know that if ribosomes are denser than nucleus. If so, then they should settle at the bottom of tube in centrifugation (following a density gradient) before the nucleus. Secondly, if ...
Only Questions's user avatar
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Why is my tissue sample coagulating during centrifugation? How can I prevent this?

I am working on extracting water-soluble compounds from a biological tissue sample. A problem I am encountering is that the sample is too cloudy with debris/large tissue pieces to filter into HPLC ...
user7264's user avatar
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I want to replicate an experiment in cell biology, and I have a question about centrifuge RPM vs. x g

https://med.stanford.edu/news/all-news/2019/10/cytoplasm-of-scrambled-frog-eggs-organizes-into-cell-like-struct.html This is the experiment which I want to replicate. In the article describing the ...
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Swing bucket for PCR

I see a swing bucket rotor for PCR trips and don't know what step is using? What difference if I use fixed - angle? In addition, Which step I can use swing bucket instead fix - angle in molecular ...
Chanh Nghiep's user avatar
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How to isolate a high proportion of available helminth ova from animal faeces/feces without damaging them?

I need to isolate large numbers (tens to hundreds of thousands) of cat (and later dog) hookworm ova from cat/dog faeces. I have a centrifuge, and various sieves with pore sizes of 20, 63, 75, 106, ...
Jasty's user avatar
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1 answer
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Centrifuge after sonication

I follow a protocol to get protein from E.coli cells after sonication. I used to grow 6 litres of large cultures and add IPTG to express the protein. I centrifuge for 10 mins at 8,000 rpm and get the ...
SHAD's user avatar
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Is there a way to remove hemoglobin from pig kidney homogenate without deactivating enzymes?

I would like to measure different enzyme activities in centrifuged pig kidney homogenate. I get out of range errors at 450 nm. The samples are still reddish after centrifugation. Is there a way to ...
Luke's user avatar
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optimization of density gradient centrifugation

I'm trying to isolate a ~320kD protein (in oligomeric form) using density gradient ultra centrifugation technique. Can any one tell me that what gradient (sucrose or glycerol or trehalose or etc) I ...
diffracteD's user avatar
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What is the sedimentation coefficient of Mycoplasma?

I have attempted to find the sedimentation coefficient of a number of bacterial species, namely E. coli and a number of Mycoplasma species, but they seemed to be rather elusive in the literature. If ...
March Ho's user avatar
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Estimating RPM to RCF in Methods from Older Papers

I'm attempting to replicate a cell biology method from a 1958 Laboratory Investigation paper. The protocol is for the isolation of an extracellular matrix protein, and a key step is a centrifugation ...
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What's the physical meaning of Svedberg unit?

Sedimentation coefficients, using a centrifuge, are expressed using Svedberg unit (symbol S, sometimes Sv). Wikipedia states that $S = 10^{-13}$ sec but I also saw in a book that actually $S = 10^{-13}...
bruno's user avatar
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Changing time and rpm of centifuge

Inspired by this question, I want to ask a question about centrifugation. Suppose a protocol says : 10 min at 2500 rpm . Can we instead centifuge for 5 min at 5000 rpm or 20 min at 1250 rpm ?
biogirl's user avatar
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