Questions tagged [dissociation-constant]

The tag has no usage guidance.

Filter by
Sorted by
Tagged with
0
votes
0answers
23 views

Dissociation constant for random collisions

Is there a good "rule of thumb" for an approximate range of $K_d$ values for two non-associating species in solution? That is, I have two species in solution which have negligible attractive potential,...
2
votes
1answer
89 views

What type of distribution should binding affinities fit?

I've been averaging Ki values from the PDSP Ki Database, and I can't help but to wonder: how I can determine p values for the binding affinities, so I can exclude anomalies in the data if need be?
7
votes
2answers
83 views

Is there a binding affinity metric for interactions not in equilibria?

I am investigating the strength of binding of a small peptide to a protein by isolating the bound version and subjecting it to collisions with gas molecules (CID mass spectrometry) to dissociate the ...
9
votes
1answer
139 views

Is bootstrapping an acceptable way to determine standard error for binding constants? [closed]

I'm measuring binding constants my system and I appreciate the usual methods of using replicates to measure standard errors and using those errors to calculate propagation of error. I'm curious if ...