Stack Exchange Network

Stack Exchange network consists of 174 Q&A communities including Stack Overflow, the largest, most trusted online community for developers to learn, share their knowledge, and build their careers.

Visit Stack Exchange

Deoxyribonucleic acid (DNA) is the carrier of genetic information, including for all known living organisms. The only known exceptions are RNA viruses.

-1
votes
0answers
15 views

Is there a physical justification on why nucleic acids store genetic information?

I am wondering if there are some physical properties of nucleic acids that make them favourable and biologically precedent for storing genetic information.
-3
votes
0answers
19 views

what would bacterial DNA look like if replication was not successful

you suspect that an antibiotic targets the bacterial replication machinery, so you conduct an experiment you add all the components needed for replication, an original DNA and a radioactively dNTPs to ...
0
votes
1answer
33 views

How much of a DNA difference is between Chicken of the Woods, and Hen of the Woods?

I've noticed that when a COW is touching the ground it turns a gray color, but when on a log/tree it's bright orange. Is there a correlation between the DNA, and where it grows?
1
vote
2answers
55 views

What is a chromosome?

I read the wikipedia article and am confused if a chromosome is the pair of two chromotoids or if each chromatoid is considered a chromosome. I've heard someone say we have 23 pairs of chromosomes ...
1
vote
1answer
34 views

Why telomere shortening slowing down cancer?

I am reading Alternative Lengthening of Telomeres in Mammalian Cells, about how telomeres linked to human cancers. Due to the end-replication problem,5,6 the ends of linear chromosomes shorten ...
2
votes
0answers
26 views

DNA preservation at room temperature

I am considering preserving the DNA of a family member who passed away. The funeral home offers a service called DNA Memories from a Canadian company (CG Labs). They have two options: Store the DNA in ...
1
vote
1answer
13 views

Sequencing inaccurate at the primer site

The times I have sent a sample to sequence, both the forward and the reverse primer sites, show high inaccuracy while the rest of the gene is correctly sequenced. Because of this, the sequences of my ...
2
votes
2answers
51 views

Do all cells have the same DNA?

According to genome.gov, All the cells in a person's body have the same DNA. I understand that the main reason parents and children have different genomes is because of random mixing of 2 genomes, ...
0
votes
0answers
29 views

Can someone help me with the term degeneracy?

As a bit of background, I am not a biologist, I'm just a computer programmer and entrepreneur, I mostly build software applications. Currently I'm involved in a software project with a smart business ...
-2
votes
1answer
45 views

Misuse of DNA leaks to create your clones, or children

Recently, sequenced DNA data leaks have been reported. The linked article discusses possible risks like blackmailing based on health related data. However there could be one more misuse, at least ...
-1
votes
1answer
10 views

Error frequency of DNA replication without proof reading activity of DNA polymerase?

Different domains of DNA polymerase contain different activity, like 5'->3' polymerisation and 3'->5' proof reading ...
-1
votes
0answers
30 views

How much can DNA change before a creature/species can no longer live?

I have no idea if it makes sense, but I remember hearing that the DNA of a creature or species cannot be changed more than about 40% otherwise the creature either become infertile or it dies; if it ...
5
votes
2answers
88 views

How much variation is there between DNA molecules from the same organism

If different but in tact molecules of DNA were extracted from my body, for example hair sample, blood sample, and mouth swab, how much variation would there be between DNA molecules?
1
vote
1answer
44 views

Non-coding DNA correlation with rate of evolution

Non-coding DNA can be helpful in generating useful mutations that can go on to become new features/functionality of an organism. Non-coding DNA also indirectly reduces chances of mutation of useful/...
1
vote
0answers
36 views

How to preserve tissue DNA without using formalin?

Except using formalin to preserve human corpse for autopsy. I heard from the news that technique damages the DNA in the organs Question: Suppose corpse will be investigated next by another juridical ...
1
vote
0answers
25 views

What can I research for thesis on DNA data storage from math? [closed]

I'm a math researcher, I just finished a Master on Error Correcting Codes. I recently saw a presentation on DNA-based data storage that I loved. I wish to continue my studies on (applied mathematics) ...
2
votes
3answers
159 views

Can the central dogma work in reverse?

Theoretically, is it possible to obtain the original gene from the protein’s amino acid sequence as its “template”, as in, the reverse of how gene’s codons were “templates” for the amino acid sequence ...
2
votes
2answers
64 views

How to find the amino acid in the DNA protein

3' A T A G T A C C G C A T G T A C G G G C G A G A C A T T C G A G C A T T C A T 5' This a Template DNA. How to find the number of amino acids amino acids ...
-1
votes
3answers
54 views

How is the mechanism that reads dna passed?

DNA is how a species stores genetic information. Somehow the body needs to know what to do with this information. somehow the "machinery" that translates the DNA into proteins needs to be passed along ...
-2
votes
1answer
67 views

Paper saying that humans and animals are (mostly) the same age

I'm brazilian and today I've came across this news published in a religious brazilian site. It's about a recent study that says that 9 to 10 species on Earth today, including humans, came into being ...
2
votes
2answers
28 views

What happens to the complementary base when the other undergoes a base substitution mutation?

From what I know only the base pairs A and T/U, or G and C can occur due to hydrogen bonding. So if a base substitution mutation occurs, say an A is replaced by a C on the strand, does it affect only ...
2
votes
2answers
49 views

What dictates which protein to synthesize at a given time?

I understand how DNA is replicated and how it directs the synthesis of proteins from amino acids through RNA polymerase, RNA and ribosomes. Now I want to understand how it works in the big picture. ...
2
votes
1answer
52 views

Does all cells in individual has same mitochondrial DNA?

I read that genetic code is universal except for mitochondrial DNA. However, I want to clarify (generally speaking, regarding humans especially): does all mitochondria in the same cell have almost ...
0
votes
1answer
37 views

Human genome, chromosomes

I have a very basic question, but it seems the hardest to me. So we have 46 chromosomes (23, 2 copies of each). Do all chromosomes have the same DNA? If so, does it mean that in different cells with ...
3
votes
1answer
35 views

What is the purpose of the “No DNA control”?

What is the purpose of the “No DNA control”? Recently I have done a forensic DNA lab experiment and I still cannot understand the use of “No DNA control”
1
vote
0answers
19 views

How specific are Vitamin D promotors in the DNA?

As far as I understand there are genes that get regulated by the amount of Vitamin D that comes in contact with DNA. How specific are those promoters? Are there promotors that for example only get ...
0
votes
0answers
15 views

Gibson Assembly Primers

exchange biologists, Currently having some trouble getting a Gibson assembly to work and as it's my first time working with this method I thought I'd ask for some advice. See the bottom of this post ...
0
votes
0answers
8 views

best thawing strategy for -80C frozen double-spun plasma

What's the best thawing strategy for -80C frozen double-spun plasma in order to preserve the cfDNA contained in the plasma? Looking at different web searches, it seems like slowly thawing at 4C is ...
1
vote
1answer
35 views

How does one identify a seed's species & variety?

How does one identify a seed's species & variety, could it be done by some means of DNA or by use of a microscope? Say for example, I had a pumpkin seed, I knew that it was a "small sugar pumpkin"...
2
votes
2answers
138 views

Why the genome is divided into several chromosomes and not just a single big chromosome?

In many eukaryote species, there are several chromosomes. In humans, for example, there are 23 pairs of chromosomes. Why are there several chromosomes and not just a join of all chromosomes into a ...
1
vote
0answers
31 views

How diverse are the sperm cells of an individual male due to random mutations?

Due to the sheer number of sperm cells in an individual and the rate of mutations, are they likely to be incredibly diverse and encompass most of what we see across our species? For instance would it ...
0
votes
2answers
41 views

complete DNA transfer across different organism zygotes

Consider we have a monkey zygote (i.e. single cell just fused) and a tiger zygote. If we extract the nucleus of monkey zygote and place it into tiger zygote where nucleus of tiger zygote is already ...
0
votes
0answers
18 views

How to perform TUNEL Assay for yeast?

Currently, I am working on yeast strain CEN PK and I want to carry out TUNEL Assay to detect the amount of damage upon treatment with some damaging agent. We have a kit in the name of TUNEL Assay Kit -...
0
votes
0answers
17 views

Prion Protein folding

PrPsc can promote the conversion of PrPc into PrPsc, which goes on to convert (misfold) other PrPc. How can an essentially dead protein, with NO nucleic acids in it, cause a good protein to become ...
1
vote
1answer
26 views

Where can I find the pZ189vector plasmid vector sequence?

I need this sequence since it was used in this paper https://onlinelibrary.wiley.com/doi/pdf/10.1002/em.1046. They say that the plasmid was prepared as in this paper http://www.jbc.org/content/271/16/...
3
votes
3answers
253 views

Genetic IQ from a person's DNA?

Is it possible to tell a person's genetic IQ (the IQ he/she was born with) just by examining his/her DNA?
0
votes
1answer
32 views

How does the number of chromosomes evolve through generations? [duplicate]

Mitosis is a complex mechanism that, through mutations and crossover, determines how a chromosome is composed. But at that point, the number of chromosomes is already determined: how can this number ...
1
vote
1answer
36 views

is having more copies of gene better then having it less? if it's why is it ?

I was learning about elephants rarely getting cancer and learn that elephants have 40 copies of genes while human only has 2 copies of genes each from parents. so I wonder if it's better to have more ...
0
votes
0answers
37 views

False positive in relationships tests

What are the chances of false positive in paternity, maternity and sibling DNA test? Many labs offer 99.99% accuracy in paternity test, which means 1 in 10000 random sample will pass as father.
0
votes
1answer
33 views

Chart of intra-species genetic diversity across species / phyla?

I am looking for information on how genetic diversity within a species (not between species) differs between taxa. In other words: Is the "spread" in the genetic pool, if measured, for instance, in ...
1
vote
1answer
66 views

Why can a zygote develop into all different types of cell, whereas a differentiated cell cannot? [closed]

A cell formed by the fertilization between two gametes has all the DNA and can lead the development of a human person (or other animals in other species). But other cells in a human person has also ...
1
vote
0answers
22 views

UV-lamp, DNA and RNA extraction lab

I'm equipping a lab room (~3x5 meters) that's supposed to be used for DNA and RNA extractions. I know about the basic safety precautions when working with RNA and DNA extraction (fresh gloves, ...
0
votes
0answers
22 views

Question about Neanderthal and Human DNA [duplicate]

If non African sapiens share 2% of their DNA with neanderthal, does that mean that their DNA differs from Africans by 2%? https://gizmodo.com/humans-have-even-more-neanderthal-dna-than-we-realized-...
2
votes
2answers
32 views

What determines the physical location of differentiated cells in an organism?

Exposition: As a little kid, I always thought of DNA as being a description of how to build an organism. For example a human DNA would have "Add two legs and two arms", a dogs DNA would have ...
0
votes
1answer
84 views

If evolution is true, how do all organisms have varying amount of chromosomes? [duplicate]

I thought genetic mutation changes genes and therefore evolution happens, but it doesn't explain how chromosomes are created/removed if the first organism had a set amount and now all the organisms ...
0
votes
1answer
74 views

Heat shock vs electroporation

I've been transforming E. coli via heat shock in order to insert oligonucleotides (around 50 nt); however, none of my experiments have given positive results so far. I begin to question the efficiency ...
0
votes
0answers
20 views

Hi, im trying make a work about Hayflick limit, and I have a question about the DNA replication. [duplicate]

What I do not understand is why in almost the whole literature it is written that the telomere shortening will only be on the lagging chain, although the leading chain at the beginning with the end ...
2
votes
0answers
75 views

With respect to which METRIC is my DNA 99% similar to any other person's DNA? [duplicate]

I clarified my question, by editing the title. Using the comments and links below, I have answered it myself: The answer to my question is: The similarity is between 98.4%-99.9%, depending on which ...
0
votes
1answer
94 views

Why are prokaryotic promoter sequences written 5' to 3', when transcription proceeds from 3' to 5'?

It seems that the promoter regions are written from the 5' to 3' direction. My lecture notes, for example, give the -35 and -10 promoter regions sequences like so: Wikipedia seems to agree, on their ...
5
votes
2answers
203 views

Can ribosomes read ssDNA?

My question is whether translation can be done, either naturally or artificially, through a ribosome reading (single-stranded) DNA directly. If not, I would like to know what allows ssRNA to be ...