Questions tagged [dna]

Deoxyribonucleic acid (DNA) is the carrier of genetic information, including for all known living organisms. The only known exceptions are RNA viruses.

Filter by
Sorted by
Tagged with
-2
votes
0answers
18 views

Are room temperature DNA preservation services, those marketed to consumers, effective as advertised? [closed]

My Question Is: are those commercial services described below, advertised to the general population, effective at preserving DNA for thousands of years? Perhaps sealing a lock of hair within an ...
-1
votes
0answers
26 views

How to store a person's DNA for years? [closed]

I know of a similar question here but the answer is too technical and also incomplete. My mother is old so I want to take her DNA (blood drops, cheek swab and hair) and store it in case she passes ...
-1
votes
1answer
33 views

How does the order of the pairs of cross-links in DNA determine the arrangement of the amino acids?

Quoting Richard Feynman from Chapter 3 of his book Six Easy Pieces, when he talks about DNA: Attached to each sugar along the line, and linking the two chains together, are certain parts of cross-...
-4
votes
1answer
45 views

metaphors for explaining the role of DNA in the cell [closed]

Various metaphors are used to explain the central role of DNA in a cell to laypersons. These include blueprint, recipe, catalogue, instruction manual etc. I even heard someone describing DNA as a '...
0
votes
0answers
23 views

Can CAS9 mechanism be introduced into eucariotic cells to counter viral diseases

I am not a pro in bio, so the question may be a dumb one. If an eucariotic cell DNA is modified so, that they produce Cas9 and other needed proteins, and have an artificial CRISPR cassette (that can ...
-2
votes
1answer
97 views

Some related questions about DNA differences in a single organism

If you took a DNA sample from someone's brain and that same persons liver at more or less the same time, would that DNA - all else being equal (e.g. no mutations from radiation) - be exactly the same? ...
4
votes
2answers
45 views

Why is a solution of cesium chloride used in Meselson & Stahl's DNA replication experiment?

Centrifugation involves separating particles of different sizes, masses, density and etc. In the experiment, the DNA macromolecules are suspended in a solution of cesium chloride gradient and then ...
0
votes
1answer
44 views

Self-replication in the genome in the seed

Is the genome in the seeds of the plant turned off? That is, does the DNA in the seed not self-replicate? My second question is, what are the cells feeding on inside the seed before it is planted?
-3
votes
1answer
43 views

Can Euchromatin convert into Heterochromatin?

I know that Heterochromatin can convert into Euchromatin but is the reverse possible? If yes, then How?
-1
votes
1answer
41 views

What is the definition of gene, genotype and phenotype, pre and post DNA?

i know that the definition of gene is a unit of heredity, also a sequence of nucleotides in DNA that gets copied to RNA and then proteins. Genotype is the genetic composition of an individual ...
0
votes
1answer
35 views

How does permanently modifying human DNA work? And how does it impact procreation?

So I read an article about a metabolic disease being treated with gene therapy, where they inserted corrective dna into the patient. https://www.independent.co.uk/news/science/dna-permanent-change-...
0
votes
0answers
16 views

Would an enrichment of pyrimidine runs of two of more nucleotides be an early indicator of DNA to be damaged?

I am analysing two categories of protein coding genes comparing their relative characteristics. What I am observing is that one of these, in comparison to the other, is enriched in pyrimidine runs (...
0
votes
0answers
37 views

Is synthesizing DNA in-house feasible?

I've been reading up about DNA writing, and it seems there are machines available around the five-figure US dollar mark for DNA synthesis, and I'm considering picking one up for my lab. We normally ...
2
votes
1answer
71 views

Extracting DNA from a kiwi

I have two questions regarding this experiment. Online instructions say to use 5g washing up liquid, 2g salt and 100ml tap water mixed together with a mashed up kiwi. Do I use a full kiwi ? ...
0
votes
1answer
41 views

Can we apply NGS (next generation sequencing) on DNA extracted from formalin fixed paraffin embedded tissues (FFPE)?

I'm working on the microbiome and I can only use tissues which are fixed with formalin and embedded in paraffin. Is it possible to apply NGS (next generation sequencing) on FFPE (formalin fixed ...
6
votes
1answer
147 views

What does Δcys mean after a gene name?

I am reading a paper and I have come across the following statement: "Plasmids encoding full-length NCAM140 and NCAM140Δcys, intracellular domain of NCAM140, and the NCAM140ID729–750 fragment ...
0
votes
2answers
46 views

Meaning of 5’–3’ in relation to ORFs

I was reading a paper in the journal Molecular Basis of Disease, which stated: The ORFs are arranged as replicase, and protease and major S, E, M, and N proteins which follows a typical 5’—3’ order ...
0
votes
1answer
76 views

Which was the first DNA-based genome to be sequenced?

The Wikipedia article on phi X 174 states that: The phi X 174 (or φX174) bacteriophage is a single-stranded DNA (ssDNA) virus that infects Escherichia coli, and the first DNA-based genome to be ...
3
votes
0answers
36 views

Why is my DNA band bulging?

This is the only image my the TA was able to get for us. And, we're using it for our lab report. The image isn't even ours. It's another group's image that we're sharing. But I don't understand why in ...
0
votes
1answer
24 views

Do we come to know which allele is dominant by seeing family genration tree only?

I know that a Gene has Alleles (variation) and one is Dominant over Other i.e the Other Recessive. Then I got a Thought that How can we tell whether an Allele is Dominant or Recessive...... and I came ...
-1
votes
1answer
19 views

How does the position of hydroxyl group in a nucleotide monomer affect the dehydration synthesis of nucleotides?

In dehydration synthesis of nucleotides, the hydrogen atom from the 3' carbon on the deoxyribose sugar of one nucleotide reacts with the hydroxyl group on the phosphate group of another nucleotide to ...
-1
votes
2answers
68 views

Sequence of DNA

While giving an amateur reading to DNA. I was stuck within the first few lines: Refined resolution of the structure of DNA, based on X-ray crystallography of short synthetic pieces of DNA, has shown ...
1
vote
0answers
29 views

Why can DNA tests with mixed DNA of several people not be used to detect a criminal in a database?

Scenario: Two males attack another pair of two males with a weapon. The attackers are fought off and the weapon remains. Why does the police require DNA samples of the two who have been attacked to be ...
0
votes
0answers
17 views

Empirical Measurements of Barr Body Ratio

'Both X-chromosomes in a female have an equal chance of becomes a Barr body in each cell.' Every source I can find on Barr bodies claims this and I can think of no reason for why it may be wrong. What ...
1
vote
1answer
34 views

a biochemical pathway for evaluating the effect of 3' to 5' exonuclease on the proofreading activity of DNA synthesis

Can the following oversimplified biochemical pathway for DNA synthesis serve as a model for evaluating the degree of the effect that the 3' to 5' exonuclease affinity for mismatched base pairs can ...
0
votes
1answer
45 views

What would happen if we place denatured DNA in acidic medium?

DNA can be denatured at high temperatures or in alkaline solutions. But DNA can be annealed at low temperatures. I want to ask, could it be annealed in acidic medium?
0
votes
1answer
54 views

Why don't moles repair their DNA?

Cells have mechanism to repair damaged DNA. So why don't the cells in moles (the spots), repair themselves and the moles dissappear?
-4
votes
2answers
59 views

DNA denaturation and Renaturation

If we denature dsDNA by heating it and then rapidly cooling it then what would happen? I read this question, where it was written that if we were given dsDNA sample which was completely denatured and ...
-6
votes
1answer
43 views

Can the self correction mechanisms in cells imply that the DNA code to a degree is self modifying

There is an argument that proteins coded by 20 amino acids via the DNA program has so few successful protein formation that a search never can reach the complexity of it via natural selection and ...
0
votes
0answers
28 views

Why is the 5′ end of DNA a monophosphate?

According to my textbook: While the 5′ end of a DNA strand is typically a monophosphate, the 5′ end of an RNA molecule is typically a triphosphate. Source: Biology: How Life Works, 3rd Edition How ...
-1
votes
1answer
37 views

Why do some karyotype graphs contain 46 pairs of chromosomes?

The human genome consists of 23 pairs of chromosomes. Two copies of each pair connect to each other at the centromere. Normal karyotype graphs should look like this: But some karyotype graphs contain ...
1
vote
0answers
25 views

How would one model a Neutrophil Extracellular Trap with Hookean Springs?

This is a question based in biology but involves a good amount of physics. Given that a Neutrophil Extracellular Trap (N.E.T) behaves like an aggregate of DNA strands with citrullinated Histones and ...
1
vote
1answer
64 views

Do DNA viroids exist?

Viroids are described as short circular ssRNA with no protein coating. Are there any analogous infectious particles that contain DNA instead of RNA? If DNA viroids do not exist, is there an obvious ...
3
votes
1answer
153 views

Does DNA being circular or linear directly affect the speed of DNA replication?

Let's say we have two DNA molecules of equal length, one belonging to a prokaryote and the other to an eukaryote. It's known that replication of the eukaryotic DNA is faster in this case. One clear ...
0
votes
1answer
43 views

What is the role of pyrophosphatase in RNA polymerization?

In Molecular Cell Biology (8th edition) there's a fragment in chapter 5.2 that says: The energetics of the polymerization reaction strongly favor the addition of ribonucleotides to the growing RNA ...
0
votes
0answers
20 views

What exactly is a DNA molecule? [duplicate]

Are the two strands of the double helix collectively called the DNA molecule, or is each individual strand the DNA molecule?
0
votes
1answer
32 views

Is the leading strand consistent in the same chromosome with multiple replication forks?

If a chromosome has multiple origins or replication, do those origins necessarily pick the same DNA strand as leading and the same one as lagging, or can they be of opposite orientations, sending ...
2
votes
1answer
63 views

State of extracted DNA

A well-known and commonly-done experiment is to extract DNA from strawberries or other fruit by first mashing the fruit of choice, adding the mush to a mixture of water, salt, and detergent, and then ...
0
votes
1answer
40 views

What is the copy number of a given gene in GRCh37?

sorry for the naive question, but how do I determine what the copy number is in GRCh37 for a gene with multiple CNVs? (e.g. DRD4).
0
votes
0answers
15 views

SNP Genotyping Scanning technology

The silicon bead micro-array technology for SNP scanning is a marvellous example of human ingenuity. Is anyone aware of a non-technical history of its development accessible to the lay reader? As an ...
0
votes
1answer
65 views

Are there any DNA viruses with junk DNA in them?

I know mimiviruses and pandoraviruses have orphan DNA - DNA that is not found in other species - but this is DNA that codes for proteins. I am not able to find out if they contain junk DNA. By junk I ...
4
votes
1answer
82 views

How does the DNA cross through bacterial cell wall during electroporation?

There exists a lot of literature on electroporation of Gram-positive and negative bacteria. Most of it gives an explanation that electroporation works by creating transient pores in cell membranes of ...
1
vote
1answer
49 views

Origins of transcription factors and RNA Polymerase

I was learning about transcription factors and RNA polymerase from Khan Academy to supplement Dr. Robert Sapolsky's lectures on Human Behavioral Biology. As I understand: RNA Polymerase transcribes ...
3
votes
1answer
49 views

Is this basic gene diagram correctly labeled?

I keep seeing this gene diagram, and I am not sure how to interpret it. I don't know what this diagram is called or where it was first depicted, but in the second picture, I have labeled it with what ...
-1
votes
1answer
33 views

gene inversion and DNA directionality

The directionality of the DNA goes from the 3-prime end to the 5-prime end. Thus, the inversion of a gene would connect a 5-prime to a 5-prime. How could that be? Maybe inverting a gene also ...
0
votes
0answers
22 views

Universal clock for humans contained in the telomeric sequences?

I don't know if this would make sense, but imagine that we could only suffer from natural aging (not diseases whatsoever involved). Is there an estimate of what is our natural maximum lifespan that a ...
1
vote
1answer
43 views

Does using CRISPR/Cas9 knockout need to add donor DNA in the process?

I am new to this technology and don't quite understand how it works. Hope someone can give some suggestions! Thank you.
0
votes
1answer
47 views

What animal classes has also “Like Humans” XY Chromosomes for Males and XX for Females? [closed]

Are other mammals. birds, reptiles, or even insects same to humans in that the male organisms have XY Chromoson at the end and female XX? Thanks for the ansver
-1
votes
1answer
72 views

Why does the structure of RNA change? [closed]

RNA only has one strand, but like DNA, is made up of nucleotides. RNA strands are shorter than DNA strands. RNA sometimes forms a secondary double helix structure, but only intermittently. Why does ...
1
vote
0answers
35 views

Can DNA rings, i.e. plasmids, form as Möbius strips?

I know that plasmids can be present in a coiled form, which keeps the DNA strands together when they degenerate, by forming catenases. I was wondering, however, whether it has been documented to ...

1
2 3 4 5
24