Stack Exchange Network

Stack Exchange network consists of 174 Q&A communities including Stack Overflow, the largest, most trusted online community for developers to learn, share their knowledge, and build their careers.

Visit Stack Exchange

Questions tagged [dna]

Deoxyribonucleic acid (DNA) is the carrier of genetic information, including for all known living organisms. The only known exceptions are RNA viruses.

1
vote
2answers
48 views

How do mutations with very little fitness benefit get fixed?

I have one little issue with evolutionary theory that I have not been able to understand. Suppose genetic mutations happen from generation to generation. Nevertheless, from one generation to the next ...
0
votes
1answer
41 views

Is it possible to fuse DNA from two sperms into one sperm?

Is it possible to fuse the DNA of two genetically different sperms into one singular sperm? In theory, half of the number of chromosomes in a normal sperm would come from one sperm and the other half ...
-3
votes
0answers
24 views

What tests are used to identify biological sisters? [closed]

What sort of tests are used to identify biological sisters? I have a sister that is a user on this site but we were separated when she was two years old. Is there a test that can be taken to prove ...
0
votes
1answer
61 views

DNA of identical twins

I read an article which says that identical twins have 100% similarity between their DNA, but they have different fingerprints. Does that mean the DNA is different in the fingers? What body features ...
0
votes
0answers
38 views

How dangerous can it be to publish your DNA (biologically)? [closed]

I am from India. The government here is presenting a bill related to DNA. According to the said bill, the government will collect the DNA(s) of the criminals, suspects, some accused men/women and the ...
0
votes
1answer
30 views

Improving DNA quality and yield from stool samples

I prepared genomic DNA from pig stools but the concentration was very low — about 3~4 ng/µl, when I measured it using nanodrop. The A260/A280 and ...
0
votes
3answers
68 views

Double stranded RNA Single stranded DNA

I have seen in textbooks referring to ds RNA and ssDNA. How a RNA can be double stranded and likewise how a DNA can single stranded and if they do exist why are there names not interchanged?
0
votes
0answers
19 views

Alignment sample of DNA collected from the crime scene and sample DNA collected from suspect

At a crime scene where DNA sample is collected, can smith-waterman algorithm be used to determine alignment between the DNA sample collected from the crime scene and the DNA sample of the suspect ?
1
vote
2answers
58 views

Does the DNA stick to surfaces we touch?

In case of robbery, the police visit the robbery scene and collect some samples like foot print or any item suspected to have been touched by the robber. The sample is then taken to laboratory for DNA ...
2
votes
2answers
84 views

Is it possible in modern biology to make any person's identical twin (or genetical clone) using his DNA?

I was told by a Biotech professor (who came in our college for a guest lecture) that Bio Technology has now become so advanced that if we want to make an identical twin (or Genetical DNA Twin) of a ...
0
votes
1answer
22 views

Observed to Expected CpG

Observed to Expected CpG is calculated as below : Obs/Exp CpG = Number of CpG * N / (Number of C * Number of G) where N = length of sequence. I also don't ...
0
votes
1answer
39 views

Do epigenetics determine the proteins a cell produces and therefore it's function?

I'm having trouble understanding what epigenetics is in a simple sense. How I imagine it is that if we had 2 twins with identical DNA and we let them live we will see that they'll develop differently....
5
votes
2answers
97 views

How do scientists know what part of DNA does what?

There are many genetically engineered foods and animals and I was wondering, how do scientists know which part of a DNA to cut in order to produce the desired result. For example I want to make a ...
2
votes
2answers
38 views

How do scientists transfer the specific DNA in Optogenetics?

I'm a bit new to genetic modification and I was wondering in Optogenetics (a field in neuroscience) how the scientists transfered the desired DNA strand from the light-sensitive ion channel opening ...
2
votes
0answers
38 views

Can a dividing cell that skipped DNA replication become cancerous?

Let's assume that a cell fails to replicate its DNA during the S Phase of the cell cycle. Let's also assume that the appropriate CDKs are inactive (perhaps due to mutation or lack of cyclin proteins ...
1
vote
1answer
25 views

Design rules for DNA linkers

I want to use double stranded DNA linkers to physically bind two "things" together, by grafting ssDNA on each one of them and using DNA hybridization as the locking mechanism. I do not expect the ...
4
votes
0answers
60 views

How does the new discovery that mitochondrial dna is inherited from both parents change population genetics and other dates?

This new study seem to have pretty much proven that mtDNA is inherited from both parents. I assume that this would lead to most age estimates of ancient human ...
1
vote
1answer
26 views

Will DNA nanostructures be useful for medical applications?

There is a lot of work being done on developing small nanomechanical structures and even small nanomechanical actuators built with DNA. I have heard researchers motivate this research with claims ...
1
vote
0answers
64 views

How does DNA code for the actual *shape* of individual body parts/areas (NOT segments or Hox genes)?

Just to save the trouble - I am not asking for general information on how DNA codes for proteins and definitely not how Hox genes work. I have a very good understanding of the evolutionary process and ...
3
votes
0answers
30 views

Offspring of parents with different number of chromosomes

Many papers report that dog-whelks (Nucella lapillus) show a distinct chromosomal polymorphism between populations of 2n = 24 up to 2n = 36. Could somebody please tell me how many chromosomes the F1 ...
1
vote
2answers
95 views

Is it possible to deduce facts about a person's parents just by studying his/her genome?

As an example, suppose Anne had abusive parents. Is it theoretically possible to deduce this from her genome even if she didn't inherit this quality (of being an abusive parent)?
2
votes
1answer
55 views

How much of my ancestry will match with my brother?

Recently, my brother (full sibling) got his ancestry checked from MyHeritageDNA. They have a similar service like 23 and me and I've found out that both companies are offering the basic service almost ...
1
vote
1answer
34 views

How to convert enrichment/depletion to frequency for comparing deep sequencing to sequence profile?

I have two datasets, from different sources, that I need to compare. The first set is deep sequencing results of a directed evolution experiment, where I have the naive library and selected library ...
0
votes
1answer
39 views

Why are there inverted repeats in response elements?

The following picture shows an inverted repeat sequence in a response element. Response element sequences for glucocorticoids, estrogen, and thyroid hormone show that they all contain inverse repeats. ...
0
votes
1answer
25 views

Hydrophobic interactions in the helix-turn-helix

This slide states that the second helix works to stablize the configuration of the two helixes via hydrophobic interactions. What exactly is this hydrophobic interaction? In other words, what ...
1
vote
2answers
56 views

Name of dsRNA (or dsDNA) where all strands are identical

What is the name of dsRNA (or DNA) where all component strands are identical (i.e. where the complex consists of multiple copies of the same ssRNA)? Example: 2 identical ssRNAs forming a dsRNA ...
0
votes
1answer
42 views

GC content of a protein-coding region

I have computer science background and I am trying to understand the equation below. The average value of the G + C content of a protein-coding region is given by : (G+C)= $\sum_{\alpha} (I_\alpha *...
5
votes
0answers
49 views

Realistic Application of CRISPR in Human Disorders

Human trials recently began to use the genome editing technology CRISPR to treat sickle cell anemia using edited stem cells. Sickle cell anemia is caused by a single DNA Mutation, and is also a ...
0
votes
1answer
46 views

Is there a feasible way to create a large number of long random DNA strands?

Suppose I want to create a large number of random DNA strands (maybe a million, or a billion, or even something close to all $4^N$ possible sequences of $N$ base pairs). Is there an experimental way ...
1
vote
0answers
29 views

Does every protein encoding gene necessarily have a transcription factor?

For instance, transcription factor gene A is responsible for activating gene B that encodes protein 1. However, it is possible for genes like gene B to encode proteins without having transcription ...
2
votes
1answer
23 views

How does DNA determine all of our hereditary traits?

It's my understanding that DNA codes only for protein synthesis. Does that mean that hereditary traits, like the shape of our nose, are determined only by the proportions in which various proteins are ...
2
votes
2answers
210 views

What makes/breaks the hydrogen bonds between DNA and RNA during transcription?

So I know that RNA polymerase catalyzes the phosphodiester bonds that hold the sugar backbones of a growing mRNA molecule together during transcription. However, I'm less sure about the hydrogen bonds ...
1
vote
2answers
64 views

Antiparallel DNA Strands - Error Correcting Code? [closed]

Let me quote Wikipedia.org: "The two strands of DNA run in opposite directions to each other and are thus antiparallel." There is a practice or method used for error correction with regard to ...
1
vote
1answer
24 views

Crispr complex in human cells?

Is the crispr (where the parts of Virus DNA is saved) section of the DNA existing in human cells aswell or is it just in bacteria cells?
3
votes
2answers
110 views

Chemistry of phosphodiester bond formation by DNA polymerase

As I'm teaching General Biology to my college students, I realized that I don't fully understand how a 3-P nucleotide like ATP is broken down to be incorporated into DNA during replication. How does ...
1
vote
1answer
32 views

Are there interesting biomolecules consisting of chains of nucleotides that present multiple bond?

Watson-Crick bonds guanine-cytosine and adenine-thymine helically shape DNA. If I understand correctly, a sequence in only two of the nucleotides, for instance TGGTGTGGGTG ... would determine the dual ...
1
vote
1answer
90 views

What experiment for first time showed DNA is anti-parallel? [closed]

Scientists how to prove DNA is anti-parallel?
1
vote
1answer
37 views

Passing on the DNA of old age

I've often pondered the fact that we only pass on the DNA of our youth. Any mutations to our DNA that occur after having children, that may help us to respond to getting older, are not inherited. Do ...
0
votes
1answer
58 views

How can two biological sequences be anything but homologous?

If we consider homology between two nucleotide sequences as a yes/no answer to whether they have shared a common ancestral sequence, then given that all life share common ancestry and sequences are ...
0
votes
0answers
8 views

Can Phi29 polymerase work at higer temperature?

Phi29 polymerase is often used at 25-30 degrees Celsius and heat inactivated at 60-70 degrees. I couldn't find any data on its functionality on temperatures around 40-50 degrees. Before testing it ...
1
vote
1answer
43 views

What is (roughly) the net charge of the DNA in an average human cell?

I was wondering about what general range for the net charge of DNA in human cells is in Coulombs. I imagine that kind of thing would be hard to measure but could be approximately calculated/estimated....
11
votes
1answer
163 views

Can mitochondria become cancerous?

Given that mitochondria have their own DNA and can replicate independently, can they ever become cancerous? For example, could a mutation in their DNA cause them to rapidly replicate, ultimately ...
0
votes
1answer
84 views

How are elements like iron recognized and routed to the right places for use in the body?

I asked this in Chemistry and it was suggested to ask it here. I wondered how the genome, which afaik does not contain iron or calcium as "reference" copies, nonetheless produces an organism that uses ...
0
votes
1answer
73 views

How much of a DNA difference is between Chicken of the Woods, and Hen of the Woods? [closed]

I've noticed that when a COW is touching the ground it turns a gray color, but when on a log/tree it's bright orange. Is there a correlation between the DNA, and where it grows?
1
vote
2answers
135 views

What is a chromosome?

I read the wikipedia article and am confused if a chromosome is the pair of two chromotoids or if each chromatoid is considered a chromosome. I've heard someone say we have 23 pairs of chromosomes ...
2
votes
1answer
50 views

Why telomere shortening slowing down cancer?

I am reading Alternative Lengthening of Telomeres in Mammalian Cells, about how telomeres linked to human cancers. Due to the end-replication problem,5,6 the ends of linear chromosomes shorten ...
2
votes
0answers
36 views

DNA preservation at room temperature

I am considering preserving the DNA of a family member who passed away. The funeral home offers a service called DNA Memories from a Canadian company (CG Labs). They have two options: Store the DNA in ...
1
vote
1answer
21 views

Sequencing inaccurate at the primer site

The times I have sent a sample to sequence, both the forward and the reverse primer sites, show high inaccuracy while the rest of the gene is correctly sequenced. Because of this, the sequences of my ...
2
votes
2answers
60 views

Do all cells have the same DNA? [duplicate]

According to genome.gov, All the cells in a person's body have the same DNA. I understand that the main reason parents and children have different genomes is because of random mixing of 2 genomes, ...
-2
votes
1answer
53 views

Misuse of DNA leaks to create your clones, or children

Recently, sequenced DNA data leaks have been reported. The linked article discusses possible risks like blackmailing based on health related data. However there could be one more misuse, at least ...