Stack Exchange Network

Stack Exchange network consists of 174 Q&A communities including Stack Overflow, the largest, most trusted online community for developers to learn, share their knowledge, and build their careers.

Visit Stack Exchange

Questions tagged [dna-damage]

A structural aberrancy in DNA, as opposed to a sequence change (mutation). Often caused by reactive species.

2
votes
0answers
34 views

DNA preservation at room temperature

I am considering preserving the DNA of a family member who passed away. The funeral home offers a service called DNA Memories from a Canadian company (CG Labs). They have two options: Store the DNA in ...
-1
votes
1answer
21 views

Error frequency of DNA replication without proof reading activity of DNA polymerase?

Different domains of DNA polymerase contain different activity, like 5'->3' polymerisation and 3'->5' proof reading ...
0
votes
0answers
25 views

How to perform TUNEL Assay for yeast?

Currently, I am working on yeast strain CEN PK and I want to carry out TUNEL Assay to detect the amount of damage upon treatment with some damaging agent. We have a kit in the name of TUNEL Assay Kit -...
1
vote
1answer
70 views

Why replication collapse but not stall leads to DNA break?

I have been looking into the concept of replication dynamics and was wondering why collapsing but not stalling leads to a DNA break.
3
votes
2answers
248 views

Is there a point in our life when ALL the atoms from our childhood's body get replaced?

The evolutionary biologist and Oxford professor Richard Dawkins makes on his TED talk the rather interesting suggestion that we human beings, are in nature more similar to a wave than an object. In ...
1
vote
2answers
55 views

Is it possible for a human to live forever if he/she didn't catch any diseases?

Aging is not a disease, but it's the gradual weakening of the body, as the body gets weaker it gets more prone to diseases. So one doesn't die from aging but from diseases. So if one didn't catch any ...
1
vote
2answers
193 views

Why does asbestos cause cancer?

We are probably all familiar with the dangers of asbestos in your rooftop or in various pieces of old equipment as it has been shown that the microscopic asbestos particles reach our lungs and can ...
1
vote
0answers
22 views

Non-cavitating ultrasound for denaturation of short strands of DNA (~20 bp)?

I know that there is existing research on ultrasound-induced transient cavitation bubbles that mechanically denature DNA by generating violent shock waves locally in solution. I would like to know ...
6
votes
1answer
362 views

What do white blood cells do with FOREIGN white blood cells?

White blood cells or leukocytes are known to fight invaders, infections or basically anything foreign. They also contain DNA (while red blood cells don't). But what about foreign "white blood cells" ...
1
vote
1answer
1k views

Which frequency of UV light damages DNA?

I have read that UV light can damage pure DNA (DNA that has been extracted and purified). Is there a particular frequency threshold where this damage occurs or is it more of a gradient where the high ...
0
votes
1answer
47 views

Why doesn’t p53 cause the repair of cellular DNA that has been altered experimentally?

When scientists mutate bacteria or even embryos of lambs and other animals, why doesn't the p53 reverse whatever mutation the scientists cause? I know that the p53 stops the DNA from mutating and ...
0
votes
1answer
29 views

Does the SMUG1 enzyme break single-stranded DNA?

The SMUG1 gene codes for the enzyme single-strand selective monofunctional uracil DNA glycosylase. This enzyme can remove uracil from single-strand (unpaired) DNA. (Uracil can occur in DNA due to ...
2
votes
1answer
98 views

Using UV to kill bacteria in shoes

I see that UV-C is the best to kill bacteria generally. We are interested in using to kill off odor in shoes. What illuminance is needed to kill most bacteria with UV-C? Thanks!
4
votes
2answers
97 views

How to safely conserve my current DNA methylation marks?

I read the Wikipedia article on DNA methylation Let's say I want to extract and then stock my current DNA methylation marks somewhere so that I can use it safely 20 years in the future for a medical ...
0
votes
0answers
75 views

Color of DNA, available absorption energy levels of DNA molecule, mutations

I have done a couple of DNA separations and observe that the clumped strands of DNA are white. Is that an artifact of the chemicals used in standard separations? Or is it indeed the case that the DNA ...
1
vote
1answer
67 views

Does vitamin C decrease after reacting with oxygen free radicals?

From the research that I have done, I know that antioxidants such as vitamin c donate electrons to oxygen free radicals and effectively neutralize them. They themselves do not become free radicals as ...
1
vote
1answer
90 views

Are nucleotides at the ends of DNA stripped on aging?

I had the following understanding (now after reading a popular science article seeming wrong understanding): DNA in (regular) cells (in human and some other organisms) are protected by telomers. ...
1
vote
1answer
731 views

Does the second law of thermodynamics cause aging? [closed]

What is the current understanding as to why organisms age and die? In the following explanation on Ask an Engineer, entropy is not even considered (despite it being answered by an engineer). Unless ...
5
votes
1answer
213 views

How do mutations actually occur?

DNA replication seems so mechanical- the DNA polymerase just running along the template strand. I just don't understand how mutations can arise. When it comes to substitutions, I get that a wrong ...
1
vote
3answers
2k views

Are missing limbs hereditary?

So with my basic understanding of biology, DNA can change over time. Does this mean that if one (or both) parents have something major missing (like a limb), the kid will come out without a limb? ...
1
vote
1answer
203 views

tandem repeat sequence; causes of contraction and/or expansion question

Question: Which of the following events, occurring within a tandem repeat sequence, will cause an expansion or contraction of the array? A) Endoduplication B) homologous recombination C) ...
1
vote
1answer
115 views

meaning of “usually greater than 200 base pairs”

From "Risks from GMOs due to Horizontal Gene Transfer", by Paul Keese: Homologous recombination. All cellular organisms have molecular functions dedicated to recognizing and recombining DNA ...
5
votes
1answer
866 views

How does NHEJ cause indels?

I was reading up on CRISPR-cas9 and how it works and I am having trouble wrapping my head around how NHEJ to repair the DSB can cause indels to occur. Shouldn't the NHEJ just stick the two strands of ...
2
votes
0answers
46 views

transitional mutation, repair mechanism

I have read an answer to a question about dna repair during and after replication. But how is dna repaired if a deamination of of 5-methylcytosine happens? How does a repair mechanism identify which ...
3
votes
1answer
165 views

Is each generation getting older? [duplicate]

So we know that our sperm and egg cells get set aside relatively early so that they aren't going through unnecessary cell divisions and causing DNA damage or telomere shortening, but since each new ...
2
votes
2answers
106 views

RF Cautery DNA damage?

I'm concerned about the safety and mechanism of RF cautery. The frequency of the radio waves used is 4Mhz so normally there should not be any DNA damage BUT the thing you read everywhere is that only ...
3
votes
2answers
376 views

How can we create a living dinosaur using DNA technology?

I'm wondering what would we need to do to create a living dinosaur using DNA technology? If it is not possible with current technology, will it ever be feasible? In the movie Jurassic Park, ...
5
votes
2answers
128 views

Questions on DNA damage

I'm not strong in biology, so bear with me on this: I've been reading that as we age, our DNA is damaged by internal (e.g. errors during replication) and external (e.g. sun damage or radiation) ...
5
votes
1answer
4k views

Why AZT is selective towards HIV and doesn't impair human DNA replication?

I've found this article, which is a very old one (from the time when nucleoside analogs where researched as a possible way to prevent replication of virus genetic material, before the HIV epidemics). ...
6
votes
1answer
96 views

Repair wrong DNA pair after ending replication?

If there are wrong DNA pair, for example A=G, is there mechanism that could repair such things - after the replication was finished? Or it happens only during replication?
1
vote
1answer
57 views

The damage of cancer cells

I read about the molecular biology of cancer, and I have a mess on my head and a lot of questions.. . My primary question is- The damage of the cancer cells is in the dna sequence or in the gene ...
1
vote
2answers
399 views

How does DNA damage cause ageing in yeast?

As I understand it, in yeast ageing there is daughter cell and mother cell. The daughter cell is has newly "fresh" DNA and mother cell dies after some counts of replication. What happens to the ...
2
votes
1answer
1k views

G>T transversion VS. T>G transversion?

So I'm reading about how mutations in DNA can be caused by oxidative damage. An example of a product of oxidative damage is given: 8-oxo-7-hydrodeoxyguanosine My textbook says that this product ...
3
votes
2answers
5k views

How does non-homologous end joining (NHEJ) work?

I was reading about non-homologous end joining (NHEJ) in my molecular biology of the gene textbook but the explanation provided in the text was rather vague to me, and I was not able to understand it ...
3
votes
1answer
296 views

How do nicks in the DNA strand affect the success of Long Range PCR?

Long Range PCR using NEB Master Mix - Hot Start Taq was working fine for me (amplicon sizes of ~10kb) but stopped working all of a sudden. Is it possible that many freeze-thaw cycles on the DNA ...
1
vote
1answer
263 views

Question about the DNA glycosylase enzyme assay/formamide gel loading buffer

Often for the DNA glycosylase enzyme assay we can see that the DNA glycosylase is incubated with oligonucleotide in 37C and then reaction is stopped by addition of formamide gel loading buffer (80% ...
1
vote
3answers
2k views

If everybody DNA is different then how blood can match even with matching blood group?

We know that everybody's DNA pattern is different in the world. Then how can ´we transfer blood from one person to another person and this person can survive ?
4
votes
2answers
246 views

Stay young by reinserting own DNA from years ago?

As far as I know, the DNA sequence becomes shorter every time a cell divides. A shorter sequence results in information loss and aging characteristics. As a countermeasure, shouldn't one stay young ...
8
votes
2answers
3k views

Can cancer cells in the same person, organ, and origin have different DNA?

Is it possible for cells from the same tumor to have different genetic material, and if so, to what degree is it possible (how fast do they mutate) ?
1
vote
0answers
296 views

Which is the best way to fix and preserve comet assay slides before staining with Ethidium Bromide?

I want to use the method of Single Cell Gel Electrophoresis (comet assay). I have many samples, but I don't have the appropriate microscope. So I have to preserve my slides until the time comes for ...
8
votes
1answer
11k views

Difference between mutation and DNA damage

What is the strict difference between mutation and DNA damage? As far as I understand it, a mutation is an alteration in the genetic sequence, having "tricked" the repairing machinery and thus ...
3
votes
1answer
102 views

Do mitochondrial DNA have repair enzymes?

Mitochondria are considered endosymbionts originated from a prokaryote. So, do they have a DNA repair mechanism ?