Questions tagged [dna-damage]

A structural aberrancy in DNA, as opposed to a sequence change (mutation). Often caused by reactive species.

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DNA preservation at room temperature

I am considering preserving the DNA of a family member who passed away. The funeral home offers a service called DNA Memories from a Canadian company (CG Labs). They have two options: Store the DNA in ...
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transitional mutation, repair mechanism

I have read an answer to a question about dna repair during and after replication. But how is dna repaired if a deamination of of 5-methylcytosine happens? How does a repair mechanism identify which ...
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Non-cavitating ultrasound for denaturation of short strands of DNA (~20 bp)?

I know that there is existing research on ultrasound-induced transient cavitation bubbles that mechanically denature DNA by generating violent shock waves locally in solution. I would like to know ...
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Which is the best way to fix and preserve comet assay slides before staining with Ethidium Bromide?

I want to use the method of Single Cell Gel Electrophoresis (comet assay). I have many samples, but I don't have the appropriate microscope. So I have to preserve my slides until the time comes for ...
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Can damage from stress hormones/distress be reversed?

Emotional distress is said and scientifically even validated that it could contribute to earlier aging, even aside from chronological age. Stresses also can have millions of pathways in which they can ...
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2answers
68 views

Can something cause both breaks and cross-links in DNA?

A double strand break in DNA is exactly that: the strands of DNA are severed. A cross-linkage occurs when something forms a covalent bond between two nucleotides in DNA. However, is it possible for ...
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Time span until everything biological is stabilized/finalized after a low-level exposure to ionizing radiation?

Say the x-rays of a common diagnostic CT (< = 10 mSv?). Irrespective of any ultimate effects (linear or threshold detrimental, inconsequential, or even protectively hormetic) just how long until ...
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What part of the skin is damaged under sun exposure?

How exactly does extensive sun exposure lead to skin damage and increase cancer risk. In which part of the skin is the sun doing the most damage. Is it in the epidermis or beneath ? I would like to ...
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Color of DNA, available absorption energy levels of DNA molecule, mutations

I have done a couple of DNA separations and observe that the clumped strands of DNA are white. Is that an artifact of the chemicals used in standard separations? Or is it indeed the case that the DNA ...
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Error frequency of DNA replication without proof reading activity of DNA polymerase?

Different domains of DNA polymerase contain different activity, like 5'->3' polymerisation and 3'->5' proof reading ...