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Questions tagged [dna-repair]

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What happens to the second strand in single-stranded / prime editing?

Articles about ’prime’ CRISPR editing generally state that a major advantage is that it only affects one strand… …But what happens to the second, complementary strand of the genome? Doesn’t it have ...
Kurt Hikes's user avatar
2 votes
1 answer

Can bottlenecked potato immunity defence be increased with knockouts?

I read in a Swedish newspaper advertisement some years ago that genetic intervention had been used to knock out part of the plant immunity defence. This advertisement was paid for by starch producer ...
David Jonsson's user avatar
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CRISPR/Cas9: How can inserted DNA be used as a donor for the homology-directed repair if Cas9 only creates blunt ends?

The CRISPR/Cas9 method allows new genes to be inserted. After Cas9 cuts the Target-DNA, it can use a homologous piece of DNA as a donor template for homology-directed repair. But HDR only occurs when ...
Void's user avatar
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12 votes
2 answers

Do spike-protein-based vaccines undermine the DNA repair system?

I ain't no biologist, but I came across a paper recently and tried to understand it: SARS–CoV–2 Spike Impairs DNA Damage Repair and Inhibits V(D)J Recombination In Vitro My question: Is it a correct ...
csstudent1418's user avatar
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Are highly-polyploid organisms more resistant to ionising-radiation-induced DNA damage?

Homologous recombination, although most famous for its use to mix together maternal and paternal alleles during meiosis, is most commonly used as a DNA-repair mechanism, allowing cells to repair ...
Vikki's user avatar
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Could you force meiosis in a cell that normally uses mitosis

I've read in the red queen something that suggests the DNA repair mechanisms are better in meiosis than they are in mitosis. I was wondering if occasionally we could force some of our cells that use ...
chemong's user avatar
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2 votes
1 answer

How does repair take place on leading strand in eukaryotes?

I am currently reading molecular biology of the gene by Watson to develop an understanding of the mechanism of replication and repair. Under the topic mismatch repair of DNA, they mentioned that ...
Harsimran kaur's user avatar
1 vote
1 answer

Does the SMUG1 enzyme break single-stranded DNA?

The SMUG1 gene codes for the enzyme single-strand selective monofunctional uracil DNA glycosylase. This enzyme can remove uracil from single-strand (unpaired) DNA. (Uracil can occur in DNA due to ...
mgkrebbs's user avatar
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7 votes
4 answers

In DNA repair, how is it determined which strand contains the error?

DNA replication is more accurate than transcription (or RNA replication) because mechanisms exist for proof-reading and repair of DNA, but not for RNA. Consider a segment of a DNA strand, AGTC. Its ...
Popopo's user avatar
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