Questions tagged [dna-replication]

The biosynthetic process by which copies of a DNA molecule are made.

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Why are stem cell therapies more preferred (theoretically) over current measures?

i understand that when stem cells are used to treat injuries using induced pluripotent stem cells (IPS), they can prevent the risk of having any tissue rejection and thus, there isn't a need for use ...
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DNA replication in E.coli

What is the difference between replication and to divide? My A level bio book says that it takes 20 min for E.coli to divide and in next page it's written that E.coli completes replication within ...
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In the future, it is plausible that we can change the physical appearance of a living animal by editing DNA?

http://www.independent.co.uk/news/science/revealed-scientists-edit-dna-to-correct-adult-genes-and-cure-diseases-9273555.html This article explains that scientists managed to change the DNA of adult ...
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griffith's experiment if both s and r strains are added can the r strain be isolated from blood of the mice

in the Griffith's experiment for transformation principle, when both the s strain and the r strain were injected into the blood of the mice ,could the r strain be isolated from the blood the dead mice....
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What are the issues with excessive tandem repeats in replication?

Why is it that tandem repeats like CAGCAGCAG cause primer-template misalignment and diseases like Huntingtons disease? By my understanding, too many such repeats can cause strands to form hairpins and ...
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Primer design: Calculating free energy for secondary structure

I'm attempting to automate primer design (specifically, primers for RPA, which requires longer primers [~30-35 nc] than PCR), and need to figure out how to calculate the free energy of a primer pair ...
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642 views

When does the kinetochore form?

According to Wikipedia, the outer plate... is assembled in the surface of the chromosomes when the nuclear envelope breaks down. However is makes not mention of when the inner portion of the ...
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712 views

How does RNA polymerase achieve higher processivity?

DNA polymerase uses a sliding clamp in order to replicate DNA. RNA polymerase does not require one. What allows RNA polymerase to hold on to DNA just like DNA polymerase?
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207 views

Why does deamination in the lagging strand lead to an increase in the relative number of guanine and thymine to cytosine and adenine?

My question arose from this article on Wikipedia on the GC-skew in bacterial genomes: https://en.wikipedia.org/wiki/GC_skew As far as I understood, the lagging strand (the template strand), during ...
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453 views

dNTP concentration for reverse transcription from RNA to cDNA

Does it affect the results if I use the half of dNTP amount in a reaction that it says in my protocol? I usually use 4 microlitres per reaction and this time I did a mistake and I used 2. The final ...
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Are there any particular chemicals that initiate either DNA replication or Transcription ? [closed]

When does the nucleus of a cell "know" when to bind DNA nucleotides ( for Replication ) or RNA nucleotides ( for Transcription ). From what i read, they're both structurally different and free ...
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Error frequency of DNA replication without proof reading activity of DNA polymerase?

Different domains of DNA polymerase contain different activity, like 5'->3' polymerisation and 3'->5' proof reading ...
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Unsure about definition of “n” and “C” values in mitosis

I am preparing for a Biology exam and I'm reviewing the "n" and "C" notation used in mitosis. My professor said that when the cell replicates its DNA in S phase of mitosis, we get twice the "amount" (...
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If we add only 1 primer in PCR [closed]

What would happen if we add only one primer, say forward primer, to PCR? (Image Credits: Wikipedia) As it is clear from the image that we need both forward and reverse primers to get it working (...
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How long does it take the bacterium nasuia deltocephalinicola to self-replicate? [closed]

At which speed does the Nasuia deltocephalinicola replicate itself given optimal conditions?
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In PCR what is the chemical makeup of the primer? DNA or RNA? [closed]

I'm thinking the answer is RNA. Is that right?
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Two 20 million yr old fossils of Hummingbirds found "The amazing thing about the fossil is that it's essentially a modern hummingbird [closed]

does this only mean that the Hummingbird is perfect in creation and needs no change to survive? http://news.bbc.co.uk/2/hi/science/nature/3691169.stm