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Questions tagged [dna]

Deoxyribonucleic acid (DNA) is the carrier of genetic information, including for all known living organisms. The only known exceptions are RNA viruses.

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1answer
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How do CpG islands remain unmethylated?

In most of the genome CpG sites are pretty much always methylated, but CpG islands are instead often unmethylated. This has been linked to the fact that they often are associated to transcripted genes....
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6answers
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Could Junk DNA be used as a Turing Machine by nature?

In what ways has DNA been studied to see if there a "programmable" aspect to it? Has nature produced anything resembling a Turing machine within the cell, perhaps using the "junk DNA" as its code? I ...
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4answers
5k views

What implications has the missing 2'-OH on the capability of DNA to form 3D structures?

The chemical difference between RNA and DNA is the missing 2'-hydroxyl group in the nucleotides that build DNA. The major effect of that change that I know of is the higher stability of DNA compared ...
30
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4answers
954 views

Is telomere length a reliable measure of health/lifespan?

Several companies are commercializing tests for telomere length such as this one here. I understand the basic mechanism for why telomeres shorten during DNA replication, but how good is the evidence ...
110
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4answers
104k views

Why is thymine rather than uracil used in DNA?

What is the advantage gained by the substitution of thymine for uracil in DNA? I have read previously that it is due to thymine being "better protected" and therefore more suited to the storage role ...
8
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4answers
223 views

How can I produce milligram quantities of an isotope-labeled DNA oligomer?

I'd like to produce a specific DNA sequence on a milligram-scale and 13C15N-label it. The sequence is around 35 nucleotides long, so chemical synthesis is out due to the exorbitant costs. I'm also ...
45
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2answers
4k views

How do cockroaches resist the effects of ionizing radiation?

Cockroaches are very hardy insects. It is known that, among other things, they are able to withstand bursts of ionizing radiation that would kill a human being. The explanations of this observed ...
6
votes
2answers
3k views

What conditions should I use for Gel Red staining?

What are the optimal staining conditions when using Gel Red? So far, since we have started using it, the gels ran in our lab have been of very poor quality. The bands are very blurred and often ...
55
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5answers
10k views

Life without DNA?

I'm by no means an expert in the field, merely a curious visitor, but I've been thinking about this and Google isn't of much help. Do we know of any lifeforms that don't have the conventional double-...
12
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1answer
1k views

How can you identify if a person is homozygous for a certain allele?

I've been thinking about starting a small private research project. In this project I need to find out whether a person is homozygous for a certain allele. The reason for this is that I'm really ...
10
votes
4answers
298 views

How can I avoid digesting protein-bound DNA?

I'm interested in sequencing and analyzing the bound DNA, and minimizing the amount of unbound DNA that gets sequenced through digestion. When digesting protein-bound DNA, is all of the unbound DNA ...