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Questions tagged [dna]

Deoxyribonucleic acid (DNA) is the carrier of genetic information, including for all known living organisms. The only known exceptions are RNA viruses.

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Are drones from one honey bee queen all clones?

The Honey Bee queen lays eggs that develop into worker bees (female) if she fertilizes the egg. If she does not fertilize the egg, it will develop into a drone (male). The drone only has the queen'...
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2answers
143 views

DNA code and codon

I work as a highschool teacher and I believe that in most highschool textbooks in my country there is a lost-in-translation problem involving some molecular biology basics. It has spread to the point ...
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2answers
48 views

Does anyone know if there is a term to describe the following process?

I'm actually currently studying physics but this came up in my textbook (taken from Giancoli 7th edition section 16-10): The random (thermal) velocities of molecules in a cell affect cloning. When a ...
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1answer
40 views

How does gene affect organs development in eukaryotic cells? [closed]

I'm totally new to biology and apologies if you find this basic. The central dogma insists that genetic information flows in this direction: DNA -> RNA -> proteins So far so good, it is very clear ...
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34 views

Can a Bio Mom and Daughter both be my first cousin?

DNA results, we thought the Mom would come back as my Aunt and then identify my bio dad as a result, but instead she came back as a predicted first cousin just as her daughter had
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Why may some telomeres not appear on metaphase chromosomes using Q-FISH?

There are some chromosomes that appear to have missing telomeres e.g. 2 instead of the expected 4, but there appears to be no fusions or breaks.
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1answer
74 views

Why replication collapse but not stall leads to DNA break?

I have been looking into the concept of replication dynamics and was wondering why collapsing but not stalling leads to a DNA break.
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25 views

Quantification of concentration from chromatogram paper [closed]

In an experiment, I recently isolated and purified calf thymus DNA, hydrolyzed it with perchloric acid, and then applied the hydrolyzate to a Whatman paper in a polar solvent and let it run overnight. ...
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0answers
38 views

Do cells store information other than permanent (chromosome) information

The brain stores information in neurons (i.e. neural networks), and cells store information in DNA. But with DNA, this is permanent information. There is a lot of potential temporary information in ...
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0answers
72 views

Would I expect salt precipitate on fibres of DNA in a NaCl water solution?

I just recently conducted an experiment in my biochemistry class where we had to add DNA to distilled water, isotonic saline and 2.5M NaCl. I noticed different physical aspects depending on the ...
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1answer
21 views

DNA test to prove that two grand-grand-children descend from same grand-grandparents

Can DNA test prove that two persons descend from same great-grandparents (or test where one great-grandchild and grand-child are tested)?
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3answers
133 views

Evolution as a directed graph exploration?

First of all, I am not biologist, nor good in chemistry/physics. Secondly, I am really eager to understand. So I think of a "life" (of a certain whatever being) as a space of somewhat properties. ...
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1answer
30 views

Storage DNA Condition

It is possible to store a digestion of cells with lysis buffer several days at -20 degrees and then proceed with the phenol chloroform isoamyl purification? Thanks.
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Why can't we directly use the DNA instead of the RNA in a DNA microarray analysis? [closed]

When proceeding to a DNA microarray analysis, I saw that the molecule that's taken from the cell is the RNA, which is afterwards converted to DNA by a reverse polymerase. But why not taking directly ...
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5answers
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How does DNA still work after recombination?

If you take two computer programs and randomly swapped pieces from each of them. The result is not going to work. It will just be garbage. If you take two novels and randomly swapped chapters the ...
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1answer
55 views

How long does it take for DNA to degrade

Can i still use 3-4 years old bacterial DNA template for my PCR? They were kept in -20 all this while. Will the DNA degrade?
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3answers
147 views

Does all of life on Earth use the same DNA compounds for all species? [closed]

If an organism on Earth has got DNA, would it always have C,T,G,A?
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0answers
118 views

How is the effect of proof-reading on error frequency during DNA replication determined?

An article in Nature Scitable on DNA Replication and the causes of Mutation states that: When an incorrect nucleotide is added to the growing strand, replication is stalled by the fact that the ...
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1answer
83 views

Where does DNA fit on the Chomsky hierarchy? [closed]

Since adenine pairs with thymine and guanine pairs with cytosine, this seems to imply that there's a trivial Regular Expression for DNA (i.e. it's trivially a Regular Language, which is Type 3 on the ...
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0answers
27 views

Will an illumina NGS library build work on previously denatured DNA?

I am trying to map the interaction of a small molecule with cellular genomic DNA using a Chem-seq approach (similar to ChIP-seq). The small molecule is crosslinked to DNA, and the DNA is enriched and ...
3
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3answers
79 views

can you create an offspring from ancient human's DNA?

If a DNA from an ancient human, for example the cheddar man, is still intact, can you make an offspring today using that intact DNA? https://www.theguardian.com/science/2018/feb/07/first-modern-...
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1answer
284 views

Find the length of DNA sequence? [closed]

Given $N=5\times10^3$ and mutation rate is $\mu=10^{-5}$ per site, find the length of a DNA sequence so that the probability of mutation occuring M, is greater or equal than 0.95. Is there a method ...
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1answer
44 views

How to choose right volume of DNA and SYBR Green

I'm testing fluorescence level of a sample having dsDNA, SYBR Green and milli q water but I'm facing difficulty in choosing right volume of dsDNA and cons level of SYBR Green. Details of sample: ...
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0answers
40 views

DNA synthesis in nucleus

I read somewhere that according to a research, I don't remember what the date was, that our cells stop synthesising DNA around noon and restart it around the sunset. The research told that it was ...
3
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1answer
199 views

Parallel DNA double-helices with Watson–Crick base-pairing: Why do they not occur? [duplicate]

I know that parallel DNA helices exist and are governed by Hoogsten base pairing, but why can’t they be possible with Watson-Crick pairing? In the diagram below, if we were to flip one of the strands ...
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0answers
25 views

What percentage of a complete Denisovan genomes lives on in the union of the sets of overlapping pieces in extant humans

In studies like this one mapping out distributions around the world or the analysis described in this article (suggesting a contribution from a non-Denisovan but Denisovan-like unidentified hominin to ...
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2answers
213 views

DNA replication during Mitosis

I am a bit confused. During Meiosis, DNA is replicated to form a cell with half the DNA and likely to have variations. But since the replication process of meiosis and mitosis are the same, why do DNA ...
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2answers
86 views

Dealing with Repetitive Bases in DNA?

I have been given a project involving a plasmid that contains long stretches of Adenine (60 or 120 bases each). These PolyA stretches are interrupted by the occasional G or C. I understand that ...
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0answers
29 views

What percentage of genome do slugs and scallops have in common?

We can know genetic distance of thousands of species. The OTT tree of life gives genetic distance for nearly all species. Is there a resource to compare the genomes of any sequenced animals to know ...
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1answer
24 views

Simple Autosomal Recessive question by a novice genetics student

How do you figure the percentage of offspring that will be a carrier of an Autosomal Recessive gene when the FATHER is the recessive carrier and the MOTHER is not affected (normal, no recessive)? ...
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0answers
67 views

how does environment affect methylation status in dna

There are many examples where environmental stress can induce changes in gene activation and deactivation. I find in many examples that the DNA methylation status of cytosine is changed to on or off ...
3
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2answers
50 views

Gene terminology - is one gene a concrete, single physical sequence?

Suppose you have two identical copies of the same, coding nucleotide sequence (e.g. two copies of BCL2 - a random gene I found on Wikipedia). Could you say that these are two genes (i.e. the name "...
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1answer
148 views

Questions about RNA Polymerase

I'm confused about the terminology and directions described in processing DNA to proteins in this paragraph. RNA polymerase reads a DNA template in the 3’ to 5’ direction to synthesize RNA in the ...
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2answers
87 views

Complete human rDNA sequence

I've been trying to retrieve the complete human rDNA sequence (non-spacers and spacers), which should be about 43-kb in length using Biomart, NCBI, and rnacentral, but I have only been able to find ...
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0answers
33 views

Methylation of DNA

How does the addition of methyl groups to DNA prevent the attack of endonucleases present in the cell? I have searched a lot on this topic but have not found the exact chemistry behind it.
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0answers
14 views

How sensitive are our gamete for epigenetic influences?

It seems that some behavior (like stress factors) and food (eg folate) can effect the methylation of some genes (at least in some studies on animals). Some of them (especially in plants) those kind of ...
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2answers
105 views

How did humans know the process of domesticating animals?

If people in ancient times had no scientific knowledge about natural selection and Darwinism, how were they knowledgeable about animal domestication and artificial selection? What tools and ...
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1answer
69 views

Where is DNA during the process of RNA extraction using Trisure?

Where is DNA during the process of RNA extraction using Trisure described here? Given that DNA is also a nucleic acid, should I expect the isolated RNA to also contain DNA? However, Bioline claims RNA ...
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2answers
46 views

Mutations in organisms

I was researching mutations and came across an interesting question: Do mutations occur in the DNA or mRNA of an organism? Is this more opinion that science? Thanks in advance!
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1k views

In PCR what is the chemical makeup of the primer? DNA or RNA? [closed]

I'm thinking the answer is RNA. Is that right?
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42 views

DNA strand displacement and Branch migration- the same or different or interrelated?

So, recently I have begun studying DNA strand displacement and then I come across the term 'branch migration' (p.s. I am not a biology major student). What I understand is that branch migration is ...
3
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2answers
367 views

“Immortal Genes” common to all organisms?

Author Sean B. Carroll mentions that there are about 500 genes common to all organisms. They have to do with the essential DNA machinery and so forth. What are these genes? Where can I read more about ...
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1answer
202 views

Why is blood collected at crime scenes? [closed]

I have read that mature red blood cells (MRBs)do not have DNA. So I am curious why crime scene technicians collect blood. Is it to collect and amplify segments of white blood cells?
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2answers
311 views

Is DNA a single molecule? [duplicate]

It seems that biology books refer to DNA as 'a' molecule (implying that it is a single continuous uninterrupted strand) ... but in those same books chromosomes are depicted as separate objects ...
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1answer
161 views

Why would I see no bands in DNA gel electrophoresis of red blood cells?

For forensic inspection, you only obtained red blood cells from an individual and amplified for the D1S80 locus in chromosome 1. Twenty nine different alleles of D1S80 have been identified, and 435 ...
2
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1answer
299 views

Does this procedure for extracting DNA from strawberries work?

I wanted to try out this experiment for extracting DNA from strawberries that can be done at home: https://www.stevespanglerscience.com/lab/experiments/strawberry-dna/ However, while doing a bit of ...
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0answers
35 views

How much DNA does a species lose after post-polyploidization genome downsizing?

After whole genome duplication, diploidization takes place, right? A lot of changings in gene organization and expression involving genetic and epigenetic mechanisms (translocations, transpositions, ...
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31 views

what exactly is a malady called crossed nerves

My dad applied to the army in the forties and was rejected because of crossed nerves. What exactly is this malady. Would appreciate any information that might concern passing this defect down to ...
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1answer
51 views

Is there enough evidence in this paper that there is a second (mechanical) layer of information in DNA?

The following commentator writes: Mechanical cues Since the mid 80s it has been hypothesized that there is a second layer of information on top of the genetic code: DNA’s mechanical properties. ...
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1answer
829 views

Proteins in different organisms

I know that DNA is universal meaning that all organisms have the same 4 base pairs and also that the same codons code for the same amino acid in all organisms. What I am wondering is that say humans ...