Questions tagged [drosophila]
Questions pertaining to fruit flies of the genus *Drosophila*.
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How can I non-destructively blind/cover one drosophila eye for behavioural assays?
I am looking for a protocol for non-destructively covering one eye of Drosophila for a behavioural and subsequent imaging experiment I am planning. I am aware of using wax to paint over the entire eye ...
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How would you find out which gene is causing a specific phenotype in Drosophila through crosses only?
I'd recently attended an interview and was asked a question I've been trying to get to the bottom of. The question was that there is a mutation in which some smaller drosophila are only attracted to ...
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Is crossveinless in Drosophila melanogaster example of condition mutant or phenocarpy?
This is the original question -
Ques - A researcher exposed Drosophila larvae to 37°C during their growth. One of the adult flies that emerged had a crossveinless phenotype. When this crossveinless ...
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Why do flies and fruit flies exhibit two distinctive (but not exclusive) flying patterns?
I live near a forest with a canal, and often see fruit flies, they also sometimes come in our house and occasionally feed on bits of fruit. Also, sometimes I meet regular house flies here and there, ...
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Size ratio of Drosophila bifurca gametes and implication for the definition of sex
I recently learned that males of Drosophila bifurca species have gigantic sperm cells, 5.8 cm long and they only produce few hundred such cells during its lifetime. This made me wonder if the male ...
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Accessing the recombination map of Drosophila melanogaster
I'm looking for some information on the data of a recombination map of Drosophila melanogaster. In 2012, an extensive study by Comeron et al. appeared saying that they had reconstructed a ...
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DNase annotations of Drosophila melanogaster
I am familiar that the RedFly database provides annotations for 1,458 sites in the genome of Drosophila melanogaster subject to cleavage by DNase I. It seems the vast majority of those annotations ...
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How can I decrease the generation time of Drosophila melanogaster?
I have a gene deletion strain of Drosophila melanogaster made with CRIPR/Cas9, and now I have to clean the background of this strain (TM6C balancer background) by making backcrosses with wild type. ...
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Recombination Data Set
I was looking over some genetics question and came across this data set.
In the fruit fly Drosophila melanogaster there is a dominant gene b+ for grey body color and another dominant gene c+ for ...
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Can citric acid be used as fungicide in banana based fly (Drosophila melanogaster) food?
I am experimenting with a banana based fly food for better larval synchronization. Instead of propionic acid, I am going to use (added) citric acid as fungicide. Is there any downside to this approach?...
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Where can I find the data on the geographical distribution of the species of Drosophila within various areas of the world?
Let X denote an arbitrary geographic area where drosophilas occur in the wild. I will need the answers to the questions of the following type:
What is the three most occurring species of the ...
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What does "count in gene set" mean?
I am trying to understand the analysis generated by STRING.
In analysis for biological processes table, there is a column titled "count in gene set." This gives a value for example "68 of 498".
What ...
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Preprocessing microarray data to detect increased male expression variance due to dosage compensation?
I am currently working with a dataset from a large microarray experiment where one of the aims is to look for evidence of the predicted doubling of variance in expression on the X chromosome in ...
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Assays for protein or lipid content of insect pupae
Does anyone have any recommendations (based on personal experience) for rapid, cheap, accurate assays for estimating protein & lipid content of individual insect (mosquito) pupae?
So far I've ...
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What hold and inject pressure to be used in microinjection?
I am planning to set up micro-injection apparatus in our lab. Normally what hold and inject pressures are used to inject DNA in fly embryo? I couldn't find in any literature as far as I've come across....
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How to find sequencing data of a specific drosophila strain
I'm trying to correct low coverage parts of an assembly of these PacBio reads with Illumina data. According to PacBio they "have sequenced adult males from a subline of the ISO1 (y; cn, bw, sp) strain ...
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Can you freeze an ATP assay sample for later use?
I am going to use adult Drosophila to do an ATP assay (ATP determination kit A22066). The most convenient for me is to freeze the samples and perform the experiment later.
However, the protocol ...
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Is complete linkage found in all four chromosomes or only Y-chromosome of Drosophila? [duplicate]
Male Drosophila shows complete linkage. Is it observed for all four chromosomes or only the Y chromosome?
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What are these two fruit flies doing?
When taking some pictures I accidentally captured two fruit flies doing this:
The photos are unfortunately blurry, but from what I can tell, one fly approaches the other from behind, and something is ...
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How many genes does D. melanogaster have?
Obviously there is no 100% exact number, but I came across this on flybase, the gold standard for annotation. I am confused now. "Genes located to the genome", is that what I am looking for? If so, ...
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Toll Like Receptors Vs Toll Receptors
What are the major differences between them, apart from one being in humans and other in Drosophilla?
user14855
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Disadvantage of using Drosophila for breeding experiment
What should be the disadvantage of using Drosophila for experiments?
a)small size of larva.
b)short life cycle.
c)mating soon after emergence of flies.
d)large number of offsprings.
Options (b) and ...
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Through which mechanisms did the Drosophila Histone Cluster evolve?
The Drosophila Histone Cluster is a gene cluster that contains more than a hundred copies of a sequence that encodes several histone proteins. These copies are very similar.
My question is what ...
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How do sex biased genes evolve?
I am wondering how do genes become sex-biased? that is, how does a gene evolve expression which is regulated in a sex-specific manner (assuming no effect from sex-limited Y/W chromosomes).
I ...
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Measuring body size in flies/insects
"Mesothorax length (the distance from the tip of the scutellum to the
most anterior part of the mesothorax)"
From Bergland et al 2008.
Would that be this distance (green line)?
If not, how would ...
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Drosophila biarmipes gene annotation, transposable element?
When doing an annotation of a contig31 of Drosophila biarmipes genome, the gene predictions(in orange, red, green, tan, blue, brown, and gray) picked up numerous possible genes in the area circled in ...
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Chromosome picture, how to interpret?
In this guide i was reading for annotations, there is a diagram of chromosomes of D.mel, I am confused as to why the chromosomes are all attached to one another, is this picture taken in a certain ...
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Degree of dosage compensation in Drosophila melanogaster
In this paper the authors state that the dosage compensation seen in Drosophila is approximately twofold, but they do not provide any source or numbers (as far as I can see) for this. What is the mean ...
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Is recombination suppressed in the males of all Drosophila species? Any other species?
Following up from this question, I am wondering if the phenomenon of genome-wide suppressed male recombination is limited to Drosophila melanogaster or is common to all Drosophila? The answer to the ...
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Crossing white eyed Drosophila with sepia eye
So, I'm doing some drosophila experiments with my high schoolers and I had really poor production of wild type individuals for doing the experiment resulting in not enough to cross with everyone... I ...
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How much total RNA can be extracted from Drosophila brain
I am wondering how much total RNA could be extracted from a single D. melanogaster brain.
I could not find this information from the literature.
The closest hit was this paper, that claims that 16-21....
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Can microdialysis be made in Drosophila melanogaster?
I've asked this before in stack overflow's cognitive science community, and someone recommended me to ask here:
I've found a couple of studies using microdialysis on insects, but didn't found any in ...
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Looking for protocol to produce a drosophila primary cell line
I'm looking for a general (and easy, this is going to be for an undergrad class) protocol to isolate and culture primary cells from fruit flies.
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Heterochromatin binding sites position information
I am working on a project and I'm trying to find data on the position of genes and heterochromatin binding sites such as HP1 in Drosophila melanogaster. Is this information available for the DGRP ...
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Have we ever observed two drosophila lineages that evolved reproductive isolation in labs?
Background
The standard definition of species refers to the concept of reproductive isolation. If two lineages are found to be reproductively isolated, then we consider these two lineages to belong ...
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Drosophila Crosses
I have 2 transgenic lines of Drosophila. I would like to cross them and get both transgene in the same fly. To start this cross, does it matter which fly is female or male? Or as long as the female is ...
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Drosophila melanogaster eclosion
Is there any published data which shows the number of Drosophila melanogaster fruit flies eclosing (hatching out of the pupae) against time since egg oviposition? I'm thinking there should be ...
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Studying the fruitfly nervous system
I'm studying Neuroscience and Fruit Flies are a pretty big deal but I don't know as much as I think I should about them. Are there and comprehensive sources that I could read?
I'd like a review of ...
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Measuring fitness / lifetime reproductive success (LRS) in Drosophila
I am planning a fitness assay of Drosophila melanogaster. I'd like to get a good measure of lifetime reproductive success (lrs) but I don't want to count all the offspring produced over a lifetime by ...
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Non-fatal / low-harm measurement of Drosophila traits
I am trying to generate some candidate traits to measure in a fitness assay of wildtype outbred (lab population) flies. The key trait I will measure is lifespan.
I am looking for some additional ...
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What makes drosophila eyes red? and is it stable?
I have Drosophila melanogaster which I am doing an eye pigmentation assay on in the future. To do this I will dissolve the heads, 10 of them removed from frozen whole flies, in acidified ethanol for ...
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What are polytene bands, and why are they there?
Drosophila polytene chromosomes have been particularly useful in genetic research, as it made cytogenetic gene mapping possible with very little effort. This was primarily accomplished due to polytene ...
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Software for counting fly eggs
Is there any software that could be used to rapidly (quicker than humans) count the eggs laid by Drosophila on a substrate by placing them under a microscope with a camera attached? The eggs are laid ...
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Combining gene expression data from two species
I currently have two sets of gene expression data. The first is a dataframe of genes identified by Annotation id CG numbers (for example "CG10005") in one column and a numerical variable of interest ...
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Why doesn't recombination occur in male Drosophila?
"Males do not show meiotic recombination, facilitating genetic studies."
For a while I have known that this phenomenon occurs, this quote comes from the Wikipedia page on Drosophila melanogaster, and ...
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