Questions tagged [ecoli]

Escherichia coli, a gram-negative, rod-shaped bacterium commonly found in the lower intestines of warm-blooded animals. Used extensively in laboratory research.

Filter by
Sorted by
Tagged with
1
vote
1answer
409 views

Effect of pH on life? Use buffer or HCl? How about lactic acid?

I'm trying to find the effect of pH on living things like E. coli and such. At first I thought of using a buffer solution which maintains its level of pH, but it's possible that the inactive ions of ...
2
votes
2answers
4k views

The antibiotic 'kanamycin' in the growing of culture E. coli

I'm a first year student and I'm not sure if I understand correctly the role played by the antibiotic 'kanamycin' in the growing of culture E. coli (Rosetta). Can anybody explain me why we use '...
1
vote
1answer
2k views

How to obtain bacteria samples?

Would anyone know how to go about obtaining samples of Salmonella or E. coli for purely educational purposes? Note: I just want to look at the stuff under a microscope.
2
votes
2answers
124 views

E. coli, risk of infection?

There has been a warning about E. coli contaminated water in South Florida. Now I'm wondering are there empirical data or historic cases which show a correlation between E.coli levels in tap water and ...
5
votes
2answers
968 views

Survival of E. coli on agar plate at -20 degree C without glycerol stock

If I kept E. coli transformed with plasmid at -20˚C without making glycerol stock, will it survive? Actually I have kept it this way for 18 hours. What will happen with it? Will it grow in fresh media?...
2
votes
1answer
72 views

Salvaging a plasmid from a cell culture stored at the incorrect temperature

I have E. coli with transformed plasmid on agar in a vial. It has been stored at -20 degree C without glycerol stock for 18 hours. This is a continuation of: Survival of E. coli on agar plate at -20 ...
5
votes
2answers
10k views

What is the appropriate method to send a strain?

We are planning to send an ecoli strain to another lab. What is the appropriate way to do so. No MTA is required but the options seem to be using filter paper or sending a glycerol stock on dry ice.
5
votes
3answers
325 views

Measuring reverse transcriptase activity in E. coli

Goal: To quantify the HIV-1 reverse transcriptase (RT) activity for a number of mutated RTs. Idea: Put RT into a vector in E.coli. Deliver GFP mRNA to E.coli. RT will process the mRNA. The ...
9
votes
1answer
335 views

How long does it take for E. coli to shift feedstocks?

With our fermentations we're noticing that it takes an appreciable amount of time for E. coli (K12 variant) to change from being metabolically streamlined on amino acids to being metabolically ...
6
votes
1answer
341 views

Which cryogenic vials and caps are ideal for storing glycerol stocks?

There are internally threaded, externally threaded, natural caps. For the vials, there are skirts, star-footed, and various bottoms. Why do any of these things matter?
8
votes
1answer
633 views

How do you knockout an E. coli gene without disrupting the rest of the gene cluster?

I'm familiar with the method to build Keio collection using single-gene deletion with an antibiotic cassette. However, what happens when there are gene clusters or overlapping ORFs and you still only ...