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Questions tagged [elisa]

ELISA stands for enyzme-linked immunosorbent assay. It is analytical method which binds an antigen to an antibody in a microreaction plate und uses a second, enzyme-linked antibody for the detection.

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656 views

Calculating CV for intra-assay ELISA

Sorry if this is in the wrong thread. I have to calculate CV for intra-assay ELISA sample OD's. My problem is that i'm finding people are using sample standard deviation in their CV formula, though ...
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What is the composition of a standard diluent buffer in a leptin ELISA kit?

I am trying to find the composition of the diluent buffer used for the dilution of a human leptin stock to be used in an ELISA assay. The manufacturer of the kit (https://www.thermofisher.com/elisa/...
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Getting Started with ELISAs

I'm interested in designing an ELISA to detect the presence of Aflatoxin B1, but I'm having a hard time finding information about designing an enzyme and some sort of guidance on how ELISA protocols ...
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63 views

Can experiments in ELISA kits be monitored via both fluorometry and photometry?

In my bioanalytics course slides, the professor has written at one point that in a heterogenous immunoassay such as ELISA, we use fluorimetry to measure concentration of an analyte. In another slide ...
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28 views

the range of interleukin 2 and 12 that can be used to activate nk-92 cells

I am one of the biotech students. I want to know the dynamic ranges of interleukin 2 and 12 that i can use to activate the NK-92 Cells. thank you
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260 views

Concentration calculation of Factor VIII for Elisa

I have the following problem. I'm trying to get the concentration calculation for an Excel sheet that will calculate values from an Elisa plate. The basis is a standard curve with the following ...
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76 views

Rationale behind washing off unbound protein antigen in indirect ELISA

My question is with regards to the usability of the indirect ELISA in measuring protein concentration. Suppose we want to quantify the concentration of a protein. The indirect ELISA method would ...
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79 views

About sandwich ELISA

In sandwich ELISA the Fc region of primary antibody bind to the polystyrene coated well. But what are the specific interactions (like 'hydrophobic interaction' or 'van der waals force') happen between ...
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5k views

What is the advantage of indirect ELISA over direct one?

I guess the answer is about indirect one giving less error due to selectivity but how exactly does that happen?
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Can ELISA be used to detect a plant enzyme? Creating assay for a new enzyme

If the goal is to generate a rapid assay for an enzyme of plant source what are the typical options? i.e. Could one do something like: Generate an antibody to the enzyme and then use it to create an ...
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5k views

Background correction when reading ELISA with TMB substrate

I'm doing some ELISA development, and I'd like some justification/best practices for background correction. I'm using a horseradish-peroxidase (HRP) detection system along with a TMB substrate and an ...
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243 views

Identifying whether ELISA kits are authentic or fake

What methods can be used to determine whether they are authentic or fake?
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How to use mechanical microstrainer to extract tissue proteins from human? [closed]

Background: There are many methods to extract proteins form human tissues out there. The majority of them use an extraction buffer containing variable concentrations of detergents and protease ...
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What would be the best design for spike-and-recovery and linearity-of-dilution validation experiments in one 96-well ELISA plate?

I have searched in the web for a detailed explanation of doing such validation experiment, but unfortunately couldn't find a satisfactory one. I came across the following sources: Thermo Scientific ...