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Questions tagged [elisa]

ELISA stands for enyzme-linked immunosorbent assay. It is analytical method which binds an antigen to an antibody in a microreaction plate und uses a second, enzyme-linked antibody for the detection.

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What would be the best design for spike-and-recovery and linearity-of-dilution validation experiments in one 96-well ELISA plate?

I have searched in the web for a detailed explanation of doing such validation experiment, but unfortunately couldn't find a satisfactory one. I came across the following sources: Thermo Scientific ...
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What is the advantage of indirect ELISA over direct one?

I guess the answer is about indirect one giving less error due to selectivity but how exactly does that happen?