Questions tagged [elisa]

ELISA stands for enyzme-linked immunosorbent assay. It is analytical method which binds an antigen to an antibody in a microreaction plate und uses a second, enzyme-linked antibody for the detection.

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Getting Started with ELISAs

I'm interested in designing an ELISA to detect the presence of Aflatoxin B1, but I'm having a hard time finding information about designing an enzyme and some sort of guidance on how ELISA protocols ...
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ELISA troubleshooting

Hi, Why does my elisa plate wells gives a brown color after adding the stop solution. I am giving a 4*5 minutes wash after each step. TMB gives it a proper blue color but when I add the stop solution ...
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Calculating CV for intra-assay ELISA

Sorry if this is in the wrong thread. I have to calculate CV for intra-assay ELISA sample OD's. My problem is that i'm finding people are using sample standard deviation in their CV formula, though ...
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What is the composition of a standard diluent buffer in a leptin ELISA kit?

I am trying to find the composition of the diluent buffer used for the dilution of a human leptin stock to be used in an ELISA assay. The manufacturer of the kit (https://www.thermofisher.com/elisa/...