Questions tagged [eukaryotic-cells]
Organisms whose cells contain organelles, complex structures enclosed within membranes, in particular a nucleus.
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How can I easily distinguish between eukaryotic and prokaryotic cells using flow cytometry?
I am trying to count populations of cells in a co-culture. One organism is Pichia pastoris and the other organism is a gram-negative bacterium. Pichia stains gram-positive using crystal violet and the ...
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Do eukaryotes assimilate DNA that is floating in the extracellular membrane?
Prokayotes, which replicate primarily using binary fission, don't get much genetic diversity. For this reason, they take in any genetic material they encounter, in a gambit to help them better adapt ...
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How stable is linear DNA transfected in eukaryotic cells?
I would like to know, with references from the literature, what is the half life of a linear dsDNA transfected in a mammalian cells.
For example, if I transfect human cells with a PCR product (500-...
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How did the nucleus of eukaryotic cells evolve?
What is/are the most popular theory/theories on how the nucleus evolved?
I know mitochondria came from alpha-proteobacteria, chloroplasts from cyanobacteria and that eukaryotes evolved directly from ...
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What is the "anatomy" of transcriptional regulation in Archaea?
I want to know what are the DNA elements that are recognized by transcription factors in Archaea and so what is the "anatomy" of transcriptional regulation in this case.
I found in this ...
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What is the difference between apoptosis caused by the release of enzymes from the electron transport chain vs that from the lysosome?
I know that the mitochondria is responsible for regulating apoptosis via release of enzymes from the electron transport chain. I believe that the release of hydrolytic enzymes from a lysosome is how ...
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Are there any barriers to telomere sequencing other than length?
In the comments to an answer on this question, it was noted that long read sequencing methods (e.g., from PacBio or Oxford Nanopore) have largely eliminated repeat-based assembly problems for bacteria....
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Difference in multicellularity between prokaryotic and eukaryotic organisms
As most prokaryotic organisms are single-celled, and in eukaryotic organisms this is the reverse, is there some evolutionary advantage that led to these features evolving? Or is it purely the fact ...
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What attaches plant cells to the cell wall?
In animal cells integrins span the plasma membrane and attach the cell membrane to the extracellular matrix. I was wondering how are plant cells attached to the cell wall? Is it just the middle ...
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Did operon evolve after endosymbiosis and specialisation of mitochondria?
We know that all extant bacteria use the operon system as their mode of regulating gene expression, in contrast to eukaryotes, which use individual gene promoters as well as epigenetic mechanisms, ...
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How many different chemical substances are there (on the average) in a human cell?
How many different chemical substances are there (on the average) in a human cell?
I saw of an estimation of ~ 1 billion (10^9) in sci-pop books.
Is there any reliable estimation rooted in the ...
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What renders a cytoplasm basophilic?
I know that being basophilic or acidophilic corresponds to affinity to certain dyes used in microscopy.
What i want to know is what characteristics of the cytoplasm can we infer from its basophilic ...
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Plants and Specialised Cells
Would the number of specialised cells in a leaf decrease if the plant was located in a shadier area? I thought it would decrease as there would be no need to store as much food in the plant and that ...
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Are there differences between the activation proteins of Eukaryotes and those of Prokaryotes
I'm in BIO 203 (for reference to my skill level), and I noticed the textbook makes a whole section out of transcriptional activator proteins, their function and applications in eukaryotes, but in ...
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"Free space" volume in the nucleus
Take a mammalian cell, say a HeLa cell for concreteness. If a molecule (e.g. a transcription factor) enters the nucleus, what is the effective volume that it can explore/access there by free diffusion?...
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About genomic imprinting
In the context of genomic imprinting, how does a human cell "know" whether a chromosome is paternal or maternal(out of a homologous pair), in order to silence genes?
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Need either a [similar] ribosome to the following few | a heuristic for finding [similar] macromolecule given 20 others
Background :
Hi! I am running a small experiment dealing with structural heterogeneity of the ribosome, actually of ribosomes across all domains of life. It's entirely computational: I get cryoEM ...
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Can plasmodial slime molds be infected by virus?
According to the definition, plasmodial slime mold consist of a "single cell" with thousands of nuclei. How would such a unique type of cell response to viral infection (if any)? Google search yield ...
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What is the difference between a macrophage and dendritic cell?
I am aware that both these cells (monocytes) are phagocytes and are able to present antigens upon their own Class II - MHC integral proteins.
Other than their difference in structure (size and shape), ...
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