Questions tagged [eukaryotic-cells]
Organisms whose cells contain organelles, complex structures enclosed within membranes, in particular a nucleus.
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Why are Chromosome Territories important?
Chromosomes occupy discrete regions of the nucleus, referred to as 'Chromosome Territories'. This spatial organization is emerging as a crucial aspect of gene regulation and genome stability in health ...
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What metabolically happens when an egg fuses with the nucleus of a somatic cell
In stem cell biology, it is recognized that embryonic stem cells are transcriptionally inactive for the first 3 days of development. However, during somatic cell nuclear transfer, the nucleus is ...
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In plant cells, is water stored only in the vacuole?
In the turgor of plant cells, is water stored only in the vacuole or can it be stored in the cytoplasm and the other cellular organs too?
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About genomic imprinting
In the context of genomic imprinting, how does a human cell "know" whether a chromosome is paternal or maternal(out of a homologous pair), in order to silence genes?
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Why is a cell in anaphase (without a nuclear envelope) be considered as a eukaryotic cell?
Can anyone shed some light on this? All I can think of is that it has something to do with the chromosomes being paired
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Do larger unicellular eukaryotic organisms have larger organelles?
It seems that many of the giant unicellular eukaryotic organisms (size 1mm and above) are multinucleate but there are some with a single nucleus as well (the genus of Acetabularia). My question is: do ...
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Need either a [similar] ribosome to the following few | a heuristic for finding [similar] macromolecule given 20 others
Background :
Hi! I am running a small experiment dealing with structural heterogeneity of the ribosome, actually of ribosomes across all domains of life. It's entirely computational: I get cryoEM ...
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Haploid eukaryotes?
I was wondering whether there are any eukaryotes which never have a diploid phase. I can't think of any. Fungi have diploid stages, and I know any sexually reproducing organisms will have at least ...
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How does repair take place on leading strand in eukaryotes?
I am currently reading molecular biology of the gene by Watson to develop an understanding of the mechanism of replication and repair.
Under the topic mismatch repair of DNA, they mentioned that ...
another 'Homo sapien'
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Osmotic gradient across cell membranes
In a typical cell in the human body, is the intracellular fluid typically hyper- or hypoosmotic to the extracellular fluid, e.g., is the osmolality of cytoplasm higher or lower than that on the ...
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Can plasmodial slime molds be infected by virus?
According to the definition, plasmodial slime mold consist of a "single cell" with thousands of nuclei. How would such a unique type of cell response to viral infection (if any)? Google search yield ...
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What happens to embedded membrane proteins after a vesicle is formed?
When an animal cell is going through endocytosis it cell surrounds a food particle, and the membrane swallows it, creating a vesicle within the cell.
However, what happens to the embedded ...
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How many different chemical substances are there (on the average) in a human cell?
How many different chemical substances are there (on the average) in a human cell?
I saw of an estimation of ~ 1 billion (10^9) in sci-pop books.
Is there any reliable estimation rooted in the ...
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What renders a cytoplasm basophilic?
I know that being basophilic or acidophilic corresponds to affinity to certain dyes used in microscopy.
What i want to know is what characteristics of the cytoplasm can we infer from its basophilic ...
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Did Plant Cells Evolve from Animal, Protist, or Fungal Cells?
I know protists and animals preceded plants but I am unaware of when fungi arose in relation to plants. At the moment, I cannot find a resource stating how plants evolved from existing kingdoms, or ...
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DNA replication - how many times and when does it occur?
I’m currently learning about DNA replication in both prokaryotic and eukaryotic cells. And my lecturer has mentioned that replication is a once in a lifetime activity. And I’m not sure what this is ...
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What organelles are in an onion cell?
I was wondering what organelles are in an onion cell, because, based on the labs we are doing in my biology class, I only saw a nucleus and cell wall. My friends and brother say there are all the ...
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How stable is linear DNA transfected in eukaryotic cells?
I would like to know, with references from the literature, what is the half life of a linear dsDNA transfected in a mammalian cells.
For example, if I transfect human cells with a PCR product (500-...
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Linear and Circular DNA
So school has taught us that eukaryotes have DNA that is linear and inside of a membrane - called the nucleus. And that prokaryotes have circular DNA that is free floating inside of the cell.
We ...
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L-selectin in white blood cells
The white blood cells' function is mainly to fight off external antigens. However, another one of its traits is its ability to bind to vascular endothelial cells with the help of L-selectin. What is ...
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Why are the cells the smallest unit of life? [closed]
Please Tell me why the cells are called the smallest unit of life. According to me I know that they are called so because they are smallest entity which is alive that is they show the properties of ...
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How long does each of the stages in meiosis take?
For each stages of meiosis (i.e. Interphase, Prophase I, ...), I wanted to know the time between each stages either in percentages or minutes. However, while I could find the cell cycle for mitosis ...
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How much DNA do humans have?
A normal human body has approximately 3 billion base pairs. Given that humans have 10 trillion cells, how is that the number of base pairs of DNA is less than the number of cells? It can happen only ...
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Neutral genetic mutations in eukaryotic cells [closed]
Why are most mutations in eukaryotic cells neutral? i.e. have no effect on the phenotype.
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Is this sentence about RER correct on Wikipedia?
While studying about Endoplasmic Reticulum on Wikipedia, I came across this sentence
A ribosome only binds to the RER once a specific protein-nucleic acid complex forms in the cytosol. This special ...
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Difference in multicellularity between prokaryotic and eukaryotic organisms
As most prokaryotic organisms are single-celled, and in eukaryotic organisms this is the reverse, is there some evolutionary advantage that led to these features evolving? Or is it purely the fact ...
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How did the nucleus of eukaryotic cells evolve?
What is/are the most popular theory/theories on how the nucleus evolved?
I know mitochondria came from alpha-proteobacteria, chloroplasts from cyanobacteria and that eukaryotes evolved directly from ...
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Why are plant cells rectangular and animal cells spherical shaped?
Is that because the plant cells have cell walls and animal cells don't, or is there a function performed by the different shapes? Perhaps round shapes assist the movement of cells?
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Why do mitochondria have a phospholipid bilayer?
So, a thought came up and I couldn't find all that much info online, so I thought I'd ask some professionals here!
The endosymbiont theory states that: mitochondria came to be ingested by bigger ...
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Why is translation so much faster in prokaryotes than eukaryotes?
Prokaryotes perform transcription and translation much faster than eukaryotes. If memory serves, a single 70S prokaryotic ribosome can incorporate around 20 amino acids per second, whereas the 80S ...
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What is the function of multiple nuclei in syncytial cells?
What is the function of multiple nuclei in syncytial cells specially in protists with cilia? Is multiple nuclei a special characteristics of only ciliated cells?
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Are there differences between the activation proteins of Eukaryotes and those of Prokaryotes
I'm in BIO 203 (for reference to my skill level), and I noticed the textbook makes a whole section out of transcriptional activator proteins, their function and applications in eukaryotes, but in ...
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Why is the rough ER necessary to make extracellular proteins? [closed]
It is my current understanding that free ribosomes synthesise proteins to be used inside the cell, and the rough endoplasmic reticulum is necessary to make proteins that are to be secreted by the cell....
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How do you differenciate between the eukaryotes and prokaryotes?
I wonder how we differentiate between the eukaryotes and prokaryotes.
Eukaryotes:
Eukaryotes (also spelled "eucaryotes") comprise animals, plants, and
fungi—which are mostly multicellular - as ...
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Are HeLa cells edible?
I'm curious if HeLa cells are intrinsically poisonous or dangerous to ingest.
My understanding is that some of the contamination in HeLa cells such as HPVs are not readily expressed.
I have no plans ...
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What attaches plant cells to the cell wall?
In animal cells integrins span the plasma membrane and attach the cell membrane to the extracellular matrix. I was wondering how are plant cells attached to the cell wall? Is it just the middle ...
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Plants and Specialised Cells
Would the number of specialised cells in a leaf decrease if the plant was located in a shadier area? I thought it would decrease as there would be no need to store as much food in the plant and that ...
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1answer
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How Mitochondria differs from Prokaryotes(probably regarding energy production)? [closed]
In other words, what evolutionary advantage is offered by mitochondria to eukaryotes(probably regarding energy production), which prokaryotes themselves cannot evolve or do?(here I am assuming that ...
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Did the Great Oxygenation Event also cause a mass extinction?
It's usually assumed that the Great Oxidation Event around 2.3 billion years ago caused a great extinction of anaerobic life on earth. However, I was reading Nick Lane's book, The Vital Question, and ...
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Do adipose cells divide in adults?
I have a dim recollection of having heard that when humans gain weight, adipose cells just get larger, rather than dividing. True?
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What determines whether the maternal or paternal allele is expressed?
I am taking cell biology and have this question:
During the process of gene expression, it is possible to express either the maternal allele or the paternal allele.
When and how is the determination ...
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1answer
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What are chiasmata?
I have a confusion in understanding what chiasmata are. My ‘NCERT’ book says ‘The beginning of diplotene is recognised by the dissolution of synaptonemal complex and the tendency of the recombined ...
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Do eukaryotes assimilate DNA that is floating in the extracellular membrane?
Prokayotes, which replicate primarily using binary fission, don't get much genetic diversity. For this reason, they take in any genetic material they encounter, in a gambit to help them better adapt ...
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1answer
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Why did eukaryotic cells develop? [closed]
If eukaryotic cells can survive in extreme conditions, then why are their still prokaryotic organisms?
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Chemoautotrophic eukaryotic cells?
There are heterotrophic prokaryotes, and there are autotrophic prokaryotes. In the autotrophic prokaryotes category, there are photoautotrophic prokaryotes and chemotrophic prokaryotes.
Are there ...
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What is the best way to express two proteins in a mammalian cell?
I have two proteins and I will be preparing a vector with both genes for stable transfection. Each protein will have their own promoter and I will use piggyBac vector to insert a single cassette with ...
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Could viruses be used as antibiotics?
Could we use viruses that only affect bacteria to act as antibiotics?
The more bacteria, the more times the virus divides, so the stronger it gets. Is this practical?
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Methods of nuclear transfection - nuclear transport
I am reading through the ENCODE papers, which is taking me well out of my comfort zone in terms of modern laboratory techniques. At the risk of asking a question which may well be thoroughly answered ...
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2answers
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How to grow fibroblast colonies from single cells?
I need to establish a cell line starting from single cells seeded on a 96 wells plate by a FACS sorter. The cells I am using are human fibroblasts RPE-1 cultured in F12 medium supplemented with 10% ...
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Is episome a plasmid or a virus?
A plasmid is a small DNA molecule that is physically separate from, and can replicate independently of, chromosomal DNA within a cell.
In general, in eukaryotes, episomes are closed circular DNA ...
