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Questions tagged [experiment]

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21 views

This 5X binding buffers where experiment participates in?

I saw 5X bingind buffer maded by; 100mM HEPES, pH 7.6 5mM EDTA 50mM ammonium sulfate 5mM DTT 1% Tween-20 150mM potassium chloride which reaction helps this buffer? I don't consider to this buffer ...
5
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1answer
102 views

What are the potential dangers (if any) facing the twin girls recently born in China with their CCR5 gene modified?

According to this Nature news article, a Chinese researcher claims to have made the world's first genome-edited baby using the popular CRISPR–Cas9 genome-editing tool. A gene called CCR5 was modified ...
2
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1answer
25 views

Fluorescent protein tags and colocalisation

We want study if 2 proteins A and B are co-located, for that we use 2 FTP(Fluorescent tag proteins) for each protein?and after the expirement these 2 FTP are co-located. Does that mean necessarily ...
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0answers
19 views

How powerful was the charge used in the original Miller-Urey Experiment?

I'm not sure "powerful" is the correct word. Basically, I want to compare the charge used with an actual lightning bolt.
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0answers
206 views

The effect of sugar on rate of yeast Respiration

In my experiment, I am investigating the effect of different types of sugar on the rate of respiration of immobilized yeast (yeast trapped in calcium alginate balls). I am looking at glucose, fructose,...
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0answers
14 views

How are siRNAs introduced to cells for RNAi experiments?

Specifically, can somebody explain how in-vitro transcription can synthesize siRNAs to conduct RNAi? I have been reading a number of research papers that discuss how to use siRNA and shRNA to conduct ...
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0answers
22 views

In a factorial design, if the effects of drug A and B are independent, how would the A+B treatment compare to A/B only?

The sample is divided into four groups, taking A and B, taking A, taking B, taking neither. I want to know what the theoretical outcome table would look like if the effects of drug A and B are ...
2
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0answers
28 views

Veritasium video about mosquitoes

In the recent veritasium video about mosquitoes , the host says that the researchers used identical female twins and fraternal female twins to understand the effect of genes on attractiveness to ...
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0answers
84 views

How do I explain enzyme specificity and reaction (lactose into glucose)?

My group did a lab where we tested milks dairy, rice, almond, and soy for glucose before and after inserting a lactase solution. Dairy tested negative before and positive after. Rice tested positive ...
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2answers
62 views

Can one conduct “novel” research without an advanced laboratory? Has it been done by school pupils? [closed]

I want to conduct an experiment but as a secondary school pupil I will be using my school’s laboratory and to make novel or interesting discoveries, wouldn’t one need advanced equipment, a prospect ...
0
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3answers
138 views

Biology Intuition and Origin of the “facts” [closed]

I am a graduate physics student but also taking biology classes. I am new to the field of bio. Most of what we do in class seem like memorizations and stating-facts. I am wondering: How can I develop ...
1
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1answer
53 views

How are fMRI responses to auditory stimuli measured?

How can fMRI experiments be conducted which measure the response to auditory stimuli (e.g. music) when the apparatus makes such loud, nasty, and distracting noises? Are there quieter MRI tomographs in ...
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1answer
61 views

bacterium ability to metabolize a compound toxic to most other organisms, do not know how the bacterium takes up the compound from the environment [closed]

A new bacterium is discovered that has a unique ability to metabolize a compound toxic to most other organisms. The microbiologists researching this bacterium do not know how the bacterium takes up ...
4
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2answers
1k views

Measuring the Rate of Photosynthesis

Update: I have just received a grant to pursue this research. However, I must still perform the preliminary experiment described below before dipping into my very limited funds. Thank you for any ...
4
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2answers
1k views

Definition of a Katal (unit of enzyme activity)

I am very confused about what one 'Katal' actually is. From Wikipedia, "The katal is not used to express the rate of a reaction; that is expressed in units of concentration per second (or moles per ...
2
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1answer
505 views

How does blue, yellow and orange coloured light affect photosynthesis? [closed]

I've been conducting an experiment with different colored light and photosynthesis, and so far my hypothesis states that red light will grow the plant, green will slow or kill it, and white will grow ...
2
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0answers
49 views

plausible science for why an enzyme breaks down a substrate better at 4 degrees rather than 20 degrees [closed]

An enzyme was found to have an optimal temperature of 20 degrees. The same enzyme in sheep liver extract was found to have an optimal temperature of 4 degrees. What are some of the factors that can ...
2
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0answers
90 views

How would you identify the independent variable in an experiment where it isn't specified? [closed]

I know that the above question is broad, but I specifically want an answer in terms of the following investigation - all of question 3 (ONLY) of this paper. The independent variable, according to ...
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0answers
483 views

Is the human eye supposed to see the lense effect?

Just as the GoPro cameras, we need lenses to be able to focus. Nevertheless, we can see the "fisheye effect" on the shots taken by GoPros. I am talking about this: Is it possible to notice the "fish ...
2
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1answer
2k views

Why does the pH decrease, when adding pancreatin to different types of milk (cow milk, soy milk etc.)?

I'm experimentally observing how pancreatin affects the pH in different types of milk. Why does the milk's pH decrease when I add an enzyme solution to different types of milk (e.g., cow milk, soy ...
3
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1answer
1k views

How do detergents interfere with protein assays?

This has been getting me stuck. I've tried to understand what a detergent would do in an assay, but I can't figure out whether it would affect the protein or the reagent (say, in a Bradford assay).
1
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1answer
1k views

Importance of fate maps

I did some normal google.book search and found the two importance of fate maps- 1. They helped establish the idea that communication between different parts of an embryo leads to the formation of new ...
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0answers
202 views

How can I test a germinating pea seed for the presence of maltose?

I need to do a simple experiment to test whether germinating pea seeds produce maltose for the breakdown of starch into simple sugars.
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0answers
404 views

Wet weight measurements: Setting up cultures for measuring growth rate via wet weight

I have some isolates which I am trying to grow in some toxic leachate in order to measure their growth rate I also want to measure the growth rates of certain combinations of these bacteria. I've ...
-1
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1answer
128 views

RNA interference and the former Petunias experiment >

I'm having problems trying to understand how RNA interference works. From what I understood, in simple terms there is this dsRNA which is fragmented into siRNA by the enzyme called Dicer, and those ...
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0answers
21 views

experimental procedure regarding AceCS1 overexpression

I'm new to biology and was just as to curious on how one can experimentally prove/disprove that AceCS1 overexpression in mammalian cells would yield enhanced rates of fatty acid synthesis through ...
3
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0answers
41k views

Experiment to show that carbon dioxide is needed for photosynthesis

In my school test, the question is as follows: Will the experiment work satisfactorily? What alterations can you make to obtain expected result? What I wrote: Yes, it will work satisfactorily. Lime ...
1
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1answer
170 views

Was the Okazaki fragment experiment ever replicated?

Prof said that the experiment "proving" the existence of Okazaki fragments was never replicated. Is this true? I tried looking on Google Scholar and I cannot find any experiments talking ...
1
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0answers
27 views

Unsure why Synechococcus moves at angle in cuvette

I'd really appreciate your help. I'm working with Synechococcus. In my experiment, I have Synechococcus in a solution of milli-Q water. I illuminated it with a focused white light through a pinhole ...
2
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1answer
725 views

Which wavelength to use to determine algae using a colorimeter?

I am doing a Biology project involving the measurements of the density of algae (Nannochloropsis) using absorbance colorimetry. What wavelength should I set my colorimeter to? The issue is that there ...
3
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0answers
38 views

How to quantify the Abundance of a particular gene in Bacteria and Archaea?

For 16s rDNA metagenomic analysis, do I need to do the following steps? DNA extraction-> Purification-> PCR amplification-> Clone -> Pick every single colony-> PCR amplify -> Sequence -> ...
4
votes
1answer
219 views

What microscope/magnification would I need to observe P. Acnes bacteria?

I am currently attempting to grow a culture of P. Acnes bacteria. Right now, my only hope in identifying colonies of the bacteria in the culture is to use a black light to find colonies that glow ...
2
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1answer
98 views

Suggestions for self-studying experimental techniques

I would appreciate any suggestion regarding self-studying experimental techniques used in biology for a theoretical scientist (candidate). Maybe I should be more specific. I'm a biology major, but ...
1
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2answers
185 views

Can we directly stimulate M cones in retina with current technology?

I wonder if we could try to see imaginary "supergreen" color by directly stimulating M cones. For this I suppose we'd need: Non-destructively scan specific human's retina to locate exact positions of ...
4
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2answers
206 views

Short, concise, practical manual for doing experimental biology

I am am physical scientist working in biology, and have recently started doing experiments. I would like a manual akin to "Numerical Recipes", but for the lab: straight forward, easy instructions on ...
4
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2answers
442 views

Transmembrane Protein Problem

Problem A transmembrane protein has 1000 aa. The 5th aa is found on the external side of the cell membrane. It interacts with the aqueous environment outside the cell. Amino acid 90 is inside the ...
2
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1answer
1k views

What causes skewed ladder bands on an agarose gel?

I frequently ran into an issue where the bands of my ladder become skewed. What causes this? Here's an example: 0.7% agarose gel, 100V, 60 minutes. Same problem occurs with 1%. I loaded 10 ul of a 20 ...
8
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1answer
2k views

How should I feed and keep an ant queen?

I have captured wandering ant queen. I guess she was just fertilised and was looking for a place to start a hive. I'd like to create my own ant hive in artificial environment. I unfortunately only ...
3
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0answers
223 views

What is this red liquid produced by a fungal colony?

In a jar, where I had some orchid plants, a fungi start growing few days ago. I removed the plants and let the fungi to expand and grow more. After some days, red drops started to appear on the ...
4
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1answer
453 views

Appropriate statistical test for a student lab?

I am a HS Bio teacher and doing a microevolution lab involving candy. Essentially students use four candy types and push them together until one cracks (Nat Sel). They also do simulations of migration,...