Questions tagged [experiment]

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Why are drug dosages so high in some mice studies?

On reading through some research on the effects of certain drugs I often come across staggeringly high dosages such as in this paper: Römer, B., Pfeiffer, N., Lewicka, S., Ben-Abdallah, N., Vogt, M. ...
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1 vote
0 answers
37 views

Why does pancreatin affect the pH of milk? [duplicate]

I just finished a lab for college and I was really intrigued by this. Since we're in a rush to finish the semester on time my professor didn't go into details much she just said something about fatty ...
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0 votes
0 answers
39 views

I am conducting an experiment with oil eating bacteria. However, I am struggling to find a method to check how much oil they cleaned?

I am experimenting to determine the optimum conditions for microbes to break down motor oil. The conditions I am going to test are different pH levels to resemble freshwater pH and saltwater pH. I ...
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2 votes
0 answers
54 views

How does Heat Fixing make the cells stick to the slide?

Today I learned how to do Gram Straining in my college and our instructor told us how we pass the slide with the bacteria on a bunsen burner 3-4 times and it fixes the bacteris so that it doesn't get ...
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3 votes
0 answers
18 views

Ventral View VS Dorsal View in cell experiments

I am trying to understand the difference between ventral and dorsal view of "cell spreading on a substrate" experiment in this article https://rupress.org/jcb/article/205/1/83/37623/A-...
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1 vote
1 answer
47 views

What is a (preferably) flying carnivorous insect that hunts adult fruit flies at a rate that doesn't threaten extinction?

I am forming an experiment, where I will be forcing evolution on Fruit Flies (Thanks rogue ant) The experiment goes a bit like this; I will be forming an ecosystem where every abiotic factor is ...
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0 votes
1 answer
25 views

Biology Experiment Algae pH & temperature help?

I am planning a Biology investigation for my IB IA on the impact of temperature and pH on algae growth. Does anyone have tips on how to measure the growth rate of algae? What algae is the most suited ...
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0 votes
1 answer
55 views

Has anyone tried the petri dish experiment with and without mask but at the side?

There are quite a few videos and experiments online that show the benefits of wearing a mask by holding a petri dish in front of their face while talking, singing, coughing etc., once with a mask and ...
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1 vote
1 answer
371 views

Why is neutralizing important when carrying out the Benedict test?

When testing for non-reducing sugars, acid is added, then the solution is boiled, then it is neutralized, benedict solution is added and then it is boiled again. Why is neutralizing the solution ...
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-1 votes
1 answer
94 views

What determines the physical appearance of a tangible amount of bacteria or viruses?

As in the title, I was wondering how would feel an amount of one type of bacteria or virus, big enough to have a tangible size of the sample. What color, state (liquid, solid), textures, smell ecc. of ...
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1 vote
0 answers
17 views

Why do nearby cells have similar meiosis periods?

A few days ago, I conducted an experiment to observe the meiosis of rye ears. During the experiment, I observed that cells in similar locations have similar meiosis period. Can anybody tell me why ...
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-1 votes
1 answer
54 views

Am I reading this respirometer correctly?

I want to double check that I'm reading these respirometer vials correctly. The volume O2 of the following vials should be: Squirrels in Torpor: E1: 0.4 E2: 5.0 E3: 0.3 E4: 5.1 E5: 0.4 E6: 0 Squirrles ...
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0 votes
1 answer
50 views

Can I imbibe seeds before sterilization?

For an experiment I want to germinate lettuce seeds. For logistical reasons it would be convenient to imbibe the seeds by placing them in the cold for 2 days, afterwards I would sterilize the seeds ...
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1 vote
1 answer
240 views

Did Mendel believe that proteins are hereditary substances?

Scientists first thought that proteins, which are found in chromosomes along with DNA, would turn out to be the sought-after genetic material. Proteins were known to have diverse amino acid sequences, ...
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-1 votes
1 answer
217 views

What data would Meselson and Stahl have expected if DNA replication was dispersive rather than semiconservative? [closed]

What data would Meselson and Stahl have expected if DNA replication was conservative rather than semiconservative? Answer: In the first generation, there would be two bands, one of light density and ...
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2 votes
0 answers
26 views

Can we see the cell cycle of the cells administered using IVIS-like device?

My question; Can we measure the cell cycle of the administered cells from outside the body, while the recipient is still alive ? Preferably, by using a device such as IVIS that can read fluorescent ...
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0 votes
0 answers
26 views

Microarray experiment validation meaning

I have a quick question: what does microarray experiment validation mean ? I was reading a paper in which they say that the data of the experiment show that the 3 genes radB, dp1 and dp2 are co-...
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2 votes
2 answers
64 views

Monitoring fish growth

I am new to experimental design and would like to perform the following experiment but I am not sure if my setup is correct or not so far. My idea is below. Let's say you want to optimise the growth ...
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5 votes
1 answer
85 views

Has there ever been a published experiment to take a viral "census" of a human subject's blood?

Has there ever been a published experiment to take a viral "census" of a human subject's blood? By a "census", I mean to identify every active virus in the subject's blood with some probability. So,...
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0 votes
0 answers
40 views

Ideas for microbiology lab. Antimicrobial properties of cutting boards

I am an undergraduate student in Biology and I am trying to design a microbiology lab that will takes 3 weeks to carry out (1-2 day each week). I am going to examine the antimicrobial properties of ...
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-1 votes
1 answer
112 views

In Griffith's experiment, if both S and R strains are added, can the R strain be isolated from the blood of the mice?

In Griffith's experiment on transformation, when both the S strain and the R strain were injected into the blood of the mice, could the R strain be isolated from the blood of the dead mice? I think it'...
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2 votes
1 answer
123 views

What advantages could there be for using the unseen variant chosen by He Jiankui instead of the naturally occurring CCR5-∆ 32 mutation?

Jennifer Doudna mentioned in https://youtu.be/9Yblg9wDHZA?t=2566 on 2019-02-21 that He Jiankui introduced an unseen variant of the CCR5 gene when gene editing the twin humans Lulu and Nana. What ...
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0 votes
1 answer
82 views

Why didn't these scientists restore electrical activity in this pig's brain?

This experiment was published in Nature Magazine: Pig brains kept alive outside body for hours after death. The researchers used a system called BrainEx to revive certain metabolic and physiological ...
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1 vote
0 answers
31 views

DNA replication in $ E.coli $

$ E.coli $ has circular DNA which I guess implies one strand forms the outer circle and the other the inner one. So, is there a way to know if the replicated DNA forms the outer or inner circle? In ...
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0 votes
0 answers
40 views

This 5X binding buffers where experiment participates in?

I saw 5X bingind buffer maded by; 100mM HEPES, pH 7.6 5mM EDTA 50mM ammonium sulfate 5mM DTT 1% Tween-20 150mM potassium chloride which reaction helps this buffer? I don't consider to this buffer ...
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12 votes
2 answers
197 views

What are the potential dangers (if any) facing the twin girls recently born in China with their CCR5 gene modified?

According to this Nature news article, a Chinese researcher claims to have made the world's first genome-edited baby using the popular CRISPR–Cas9 genome-editing tool. A gene called CCR5 was modified ...
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2 votes
1 answer
41 views

Fluorescent protein tags and colocalisation

We want study if 2 proteins A and B are co-located, for that we use 2 FTP(Fluorescent tag proteins) for each protein?and after the expirement these 2 FTP are co-located. Does that mean necessarily ...
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1 vote
0 answers
405 views

The effect of sugar on rate of yeast Respiration

In my experiment, I am investigating the effect of different types of sugar on the rate of respiration of immobilized yeast (yeast trapped in calcium alginate balls). I am looking at glucose, fructose,...
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2 votes
0 answers
77 views

Veritasium video about mosquitoes

In the recent veritasium video about mosquitoes , the host says that the researchers used identical female twins and fraternal female twins to understand the effect of genes on attractiveness to ...
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1 vote
2 answers
108 views

Can one conduct “novel” research without an advanced laboratory? Has it been done by school pupils? [closed]

I want to conduct an experiment but as a secondary school pupil I will be using my school’s laboratory and to make novel or interesting discoveries, wouldn’t one need advanced equipment, a prospect ...
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0 votes
3 answers
217 views

Biology Intuition and Origin of the "facts" [closed]

I am a graduate physics student but also taking biology classes. I am new to the field of bio. Most of what we do in class seem like memorizations and stating-facts. I am wondering: How can I develop ...
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1 vote
1 answer
58 views

How are fMRI responses to auditory stimuli measured?

How can fMRI experiments be conducted which measure the response to auditory stimuli (e.g. music) when the apparatus makes such loud, nasty, and distracting noises? Are there quieter MRI tomographs in ...
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-1 votes
1 answer
148 views

bacterium ability to metabolize a compound toxic to most other organisms, do not know how the bacterium takes up the compound from the environment [closed]

A new bacterium is discovered that has a unique ability to metabolize a compound toxic to most other organisms. The microbiologists researching this bacterium do not know how the bacterium takes up ...
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4 votes
2 answers
2k views

Measuring the Rate of Photosynthesis

Update: I have just received a grant to pursue this research. However, I must still perform the preliminary experiment described below before dipping into my very limited funds. Thank you for any ...
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4 votes
2 answers
4k views

Definition of a Katal (unit of enzyme activity)

I am very confused about what one 'Katal' actually is. From Wikipedia, "The katal is not used to express the rate of a reaction; that is expressed in units of concentration per second (or moles per ...
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2 votes
1 answer
812 views

How does blue, yellow and orange coloured light affect photosynthesis? [closed]

I've been conducting an experiment with different colored light and photosynthesis, and so far my hypothesis states that red light will grow the plant, green will slow or kill it, and white will grow ...
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2 votes
0 answers
56 views

plausible science for why an enzyme breaks down a substrate better at 4 degrees rather than 20 degrees [closed]

An enzyme was found to have an optimal temperature of 20 degrees. The same enzyme in sheep liver extract was found to have an optimal temperature of 4 degrees. What are some of the factors that can ...
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2 votes
0 answers
105 views

How would you identify the independent variable in an experiment where it isn't specified? [closed]

I know that the above question is broad, but I specifically want an answer in terms of the following investigation - all of question 3 (ONLY) of this paper. The independent variable, according to ...
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1 vote
0 answers
726 views

Is the human eye supposed to see the lense effect?

Just as the GoPro cameras, we need lenses to be able to focus. Nevertheless, we can see the "fisheye effect" on the shots taken by GoPros. I am talking about this: Is it possible to notice the "fish ...
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1 vote
1 answer
5k views

Why does the pH decrease, when adding pancreatin to different types of milk (cow milk, soy milk etc.)?

I'm experimentally observing how pancreatin affects the pH in different types of milk. Why does the milk's pH decrease when I add an enzyme solution to different types of milk (e.g., cow milk, soy ...
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2 votes
1 answer
2k views

How do detergents interfere with protein assays?

This has been getting me stuck. I've tried to understand what a detergent would do in an assay, but I can't figure out whether it would affect the protein or the reagent (say, in a Bradford assay).
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  • 2,921
1 vote
1 answer
3k views

Importance of fate maps

I did some normal google.book search and found the two importance of fate maps- 1. They helped establish the idea that communication between different parts of an embryo leads to the formation of new ...
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  • 8,754
1 vote
0 answers
433 views

How can I test a germinating pea seed for the presence of maltose?

I need to do a simple experiment to test whether germinating pea seeds produce maltose for the breakdown of starch into simple sugars.
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  • 11
1 vote
0 answers
612 views

Wet weight measurements: Setting up cultures for measuring growth rate via wet weight

I have some isolates which I am trying to grow in some toxic leachate in order to measure their growth rate I also want to measure the growth rates of certain combinations of these bacteria. I've ...
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0 votes
1 answer
205 views

RNA interference and the former Petunias experiment >

I'm having problems trying to understand how RNA interference works. From what I understood, in simple terms there is this dsRNA which is fragmented into siRNA by the enzyme called Dicer, and those ...
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1 vote
0 answers
22 views

experimental procedure regarding AceCS1 overexpression

I'm new to biology and was just as to curious on how one can experimentally prove/disprove that AceCS1 overexpression in mammalian cells would yield enhanced rates of fatty acid synthesis through ...
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3 votes
0 answers
54k views

Experiment to show that carbon dioxide is needed for photosynthesis

In my school test, the question is as follows: Will the experiment work satisfactorily? What alterations can you make to obtain expected result? What I wrote: Yes, it will work satisfactorily. Lime ...
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1 vote
1 answer
194 views

Was the Okazaki fragment experiment ever replicated?

Prof said that the experiment "proving" the existence of Okazaki fragments was never replicated. Is this true? I tried looking on Google Scholar and I cannot find any experiments talking ...
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  • 469
1 vote
0 answers
28 views

Unsure why Synechococcus moves at angle in cuvette

I'd really appreciate your help. I'm working with Synechococcus. In my experiment, I have Synechococcus in a solution of milli-Q water. I illuminated it with a focused white light through a pinhole ...
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  • 121
2 votes
1 answer
981 views

Which wavelength to use to determine algae using a colorimeter?

I am doing a Biology project involving the measurements of the density of algae (Nannochloropsis) using absorbance colorimetry. What wavelength should I set my colorimeter to? The issue is that there ...
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