Questions tagged [experiment]

The design and interpretation of procedures that test hypotheses.

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How many cuts are done during CRISPR-Cas9 in one cell?

In a CRISPR-Cas9 experiment, the protein cuts the site matching the cRNA part of the gRNA. My question is: How many cuts are possible if multiples sites matching the cRNA are found in the cell? ...
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When I use isopentane to freeze tissue, which kind of purity should I choose? GR, AR, or CP?

GR: Guaranteed Reagent AR: Analytical Reagent CP: Chemical Reagent
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What were fed to the rodents in Calhoun's experiments?

From, (https://en.wikipedia.org/wiki/Behavioral_sink) (https://en.wikipedia.org/wiki/John_B._Calhoun) (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1644264/)(https://www.gwern.net/docs/sociology/1962-...
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Methods to obtain the mass distribution of an ensemble of bacteria?

Are there any mainstream methods to obtain the mass distribution of a bacterial population? What kind of assumptions to they make? Could anyone point me to some references?
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Does beta-actin have to be consistent in Western Blots?

I have been running Western Blots on rat brain tissues from rats that have been subject to neurological disorders. I loaded the samples on the gel to ensure that for each tissue, I had a constant mass ...
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Why are drug dosages so high in some mice studies?

On reading through some research on the effects of certain drugs I often come across staggeringly high dosages such as in this paper: Römer, B., Pfeiffer, N., Lewicka, S., Ben-Abdallah, N., Vogt, M. ...
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Why does pancreatin affect the pH of milk? [duplicate]

I just finished a lab for college and I was really intrigued by this. Since we're in a rush to finish the semester on time my professor didn't go into details much she just said something about fatty ...
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I am conducting an experiment with oil eating bacteria. However, I am struggling to find a method to check how much oil they cleaned?

I am experimenting to determine the optimum conditions for microbes to break down motor oil. The conditions I am going to test are different pH levels to resemble freshwater pH and saltwater pH. I ...
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How does Heat Fixing make the cells stick to the slide?

Today I learned how to do Gram Straining in my college and our instructor told us how we pass the slide with the bacteria on a bunsen burner 3-4 times and it fixes the bacteris so that it doesn't get ...
3 votes
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Ventral View VS Dorsal View in cell experiments

I am trying to understand the difference between ventral and dorsal view of "cell spreading on a substrate" experiment in this article https://rupress.org/jcb/article/205/1/83/37623/A-...
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What is a (preferably) flying carnivorous insect that hunts adult fruit flies at a rate that doesn't threaten extinction?

I am forming an experiment, where I will be forcing evolution on Fruit Flies (Thanks rogue ant) The experiment goes a bit like this; I will be forming an ecosystem where every abiotic factor is ...
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Biology Experiment Algae pH & temperature help?

I am planning a Biology investigation for my IB IA on the impact of temperature and pH on algae growth. Does anyone have tips on how to measure the growth rate of algae? What algae is the most suited ...
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Has anyone tried the petri dish experiment with and without mask but at the side?

There are quite a few videos and experiments online that show the benefits of wearing a mask by holding a petri dish in front of their face while talking, singing, coughing etc., once with a mask and ...
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Why is neutralizing important when carrying out the Benedict test?

When testing for non-reducing sugars, acid is added, then the solution is boiled, then it is neutralized, benedict solution is added and then it is boiled again. Why is neutralizing the solution ...
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What determines the physical appearance of a tangible amount of bacteria or viruses?

As in the title, I was wondering how would feel an amount of one type of bacteria or virus, big enough to have a tangible size of the sample. What color, state (liquid, solid), textures, smell ecc. of ...
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Why do nearby cells have similar meiosis periods?

A few days ago, I conducted an experiment to observe the meiosis of rye ears. During the experiment, I observed that cells in similar locations have similar meiosis period. Can anybody tell me why ...
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Am I reading this respirometer correctly?

I want to double check that I'm reading these respirometer vials correctly. The volume O2 of the following vials should be: Squirrels in Torpor: E1: 0.4 E2: 5.0 E3: 0.3 E4: 5.1 E5: 0.4 E6: 0 Squirrles ...
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Can I imbibe seeds before sterilization?

For an experiment I want to germinate lettuce seeds. For logistical reasons it would be convenient to imbibe the seeds by placing them in the cold for 2 days, afterwards I would sterilize the seeds ...
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Did Mendel believe that proteins are hereditary substances?

Scientists first thought that proteins, which are found in chromosomes along with DNA, would turn out to be the sought-after genetic material. Proteins were known to have diverse amino acid sequences, ...
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What data would Meselson and Stahl have expected if DNA replication was dispersive rather than semiconservative? [closed]

What data would Meselson and Stahl have expected if DNA replication was conservative rather than semiconservative? Answer: In the first generation, there would be two bands, one of light density and ...
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Can we see the cell cycle of the cells administered using IVIS-like device?

My question; Can we measure the cell cycle of the administered cells from outside the body, while the recipient is still alive ? Preferably, by using a device such as IVIS that can read fluorescent ...
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Microarray experiment validation meaning

I have a quick question: what does microarray experiment validation mean ? I was reading a paper in which they say that the data of the experiment show that the 3 genes radB, dp1 and dp2 are co-...
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Monitoring fish growth

I am new to experimental design and would like to perform the following experiment but I am not sure if my setup is correct or not so far. My idea is below. Let's say you want to optimise the growth ...
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Has there ever been a published experiment to take a viral "census" of a human subject's blood?

Has there ever been a published experiment to take a viral "census" of a human subject's blood? By a "census", I mean to identify every active virus in the subject's blood with some probability. So,...
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Ideas for microbiology lab. Antimicrobial properties of cutting boards

I am an undergraduate student in Biology and I am trying to design a microbiology lab that will takes 3 weeks to carry out (1-2 day each week). I am going to examine the antimicrobial properties of ...
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In Griffith's experiment, if both S and R strains are added, can the R strain be isolated from the blood of the mice?

In Griffith's experiment on transformation, when both the S strain and the R strain were injected into the blood of the mice, could the R strain be isolated from the blood of the dead mice? I think it'...
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What advantages could there be for using the unseen variant chosen by He Jiankui instead of the naturally occurring CCR5-∆ 32 mutation?

Jennifer Doudna mentioned in https://youtu.be/9Yblg9wDHZA?t=2566 on 2019-02-21 that He Jiankui introduced an unseen variant of the CCR5 gene when gene editing the twin humans Lulu and Nana. What ...
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Why didn't these scientists restore electrical activity in this pig's brain?

This experiment was published in Nature Magazine: Pig brains kept alive outside body for hours after death. The researchers used a system called BrainEx to revive certain metabolic and physiological ...
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DNA replication in $ E.coli $

$ E.coli $ has circular DNA which I guess implies one strand forms the outer circle and the other the inner one. So, is there a way to know if the replicated DNA forms the outer or inner circle? In ...
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This 5X binding buffers where experiment participates in?

I saw 5X bingind buffer maded by; 100mM HEPES, pH 7.6 5mM EDTA 50mM ammonium sulfate 5mM DTT 1% Tween-20 150mM potassium chloride which reaction helps this buffer? I don't consider to this buffer ...
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What are the potential dangers (if any) facing the twin girls recently born in China with their CCR5 gene modified?

According to this Nature news article, a Chinese researcher claims to have made the world's first genome-edited baby using the popular CRISPR–Cas9 genome-editing tool. A gene called CCR5 was modified ...
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Fluorescent protein tags and colocalisation

We want study if 2 proteins A and B are co-located, for that we use 2 FTP(Fluorescent tag proteins) for each protein?and after the expirement these 2 FTP are co-located. Does that mean necessarily ...
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The effect of sugar on rate of yeast Respiration

In my experiment, I am investigating the effect of different types of sugar on the rate of respiration of immobilized yeast (yeast trapped in calcium alginate balls). I am looking at glucose, fructose,...
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Veritasium video about mosquitoes

In the recent veritasium video about mosquitoes , the host says that the researchers used identical female twins and fraternal female twins to understand the effect of genes on attractiveness to ...
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Can one conduct “novel” research without an advanced laboratory? Has it been done by school pupils? [closed]

I want to conduct an experiment but as a secondary school pupil I will be using my school’s laboratory and to make novel or interesting discoveries, wouldn’t one need advanced equipment, a prospect ...
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Biology Intuition and Origin of the "facts" [closed]

I am a graduate physics student but also taking biology classes. I am new to the field of bio. Most of what we do in class seem like memorizations and stating-facts. I am wondering: How can I develop ...
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How are fMRI responses to auditory stimuli measured?

How can fMRI experiments be conducted which measure the response to auditory stimuli (e.g. music) when the apparatus makes such loud, nasty, and distracting noises? Are there quieter MRI tomographs in ...
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bacterium ability to metabolize a compound toxic to most other organisms, do not know how the bacterium takes up the compound from the environment [closed]

A new bacterium is discovered that has a unique ability to metabolize a compound toxic to most other organisms. The microbiologists researching this bacterium do not know how the bacterium takes up ...
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Measuring the Rate of Photosynthesis

Update: I have just received a grant to pursue this research. However, I must still perform the preliminary experiment described below before dipping into my very limited funds. Thank you for any ...
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Definition of a Katal (unit of enzyme activity)

I am very confused about what one 'Katal' actually is. From Wikipedia, "The katal is not used to express the rate of a reaction; that is expressed in units of concentration per second (or moles per ...
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How does blue, yellow and orange coloured light affect photosynthesis? [closed]

I've been conducting an experiment with different colored light and photosynthesis, and so far my hypothesis states that red light will grow the plant, green will slow or kill it, and white will grow ...
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plausible science for why an enzyme breaks down a substrate better at 4 degrees rather than 20 degrees [closed]

An enzyme was found to have an optimal temperature of 20 degrees. The same enzyme in sheep liver extract was found to have an optimal temperature of 4 degrees. What are some of the factors that can ...
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How would you identify the independent variable in an experiment where it isn't specified? [closed]

I know that the above question is broad, but I specifically want an answer in terms of the following investigation - all of question 3 (ONLY) of this paper. The independent variable, according to ...
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Is the human eye supposed to see the lense effect?

Just as the GoPro cameras, we need lenses to be able to focus. Nevertheless, we can see the "fisheye effect" on the shots taken by GoPros. I am talking about this: Is it possible to notice the "fish ...
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Why does the pH decrease, when adding pancreatin to different types of milk (cow milk, soy milk etc.)?

I'm experimentally observing how pancreatin affects the pH in different types of milk. Why does the milk's pH decrease when I add an enzyme solution to different types of milk (e.g., cow milk, soy ...
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How do detergents interfere with protein assays?

This has been getting me stuck. I've tried to understand what a detergent would do in an assay, but I can't figure out whether it would affect the protein or the reagent (say, in a Bradford assay).
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Importance of fate maps

I did some normal google.book search and found the two importance of fate maps- 1. They helped establish the idea that communication between different parts of an embryo leads to the formation of new ...
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How can I test a germinating pea seed for the presence of maltose?

I need to do a simple experiment to test whether germinating pea seeds produce maltose for the breakdown of starch into simple sugars.
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Wet weight measurements: Setting up cultures for measuring growth rate via wet weight

I have some isolates which I am trying to grow in some toxic leachate in order to measure their growth rate I also want to measure the growth rates of certain combinations of these bacteria. I've ...
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RNA interference and the former Petunias experiment >

I'm having problems trying to understand how RNA interference works. From what I understood, in simple terms there is this dsRNA which is fragmented into siRNA by the enzyme called Dicer, and those ...