Questions tagged [experiment]
The experiment tag has no usage guidance.
51
questions
11
votes
2answers
187 views
What are the potential dangers (if any) facing the twin girls recently born in China with their CCR5 gene modified?
According to this Nature news article, a Chinese researcher claims to have made the world's first genome-edited baby using the popular CRISPR–Cas9 genome-editing tool. A gene called CCR5 was modified ...
8
votes
1answer
2k views
How should I feed and keep an ant queen?
I have captured wandering ant queen. I guess she was just fertilised and was looking for a place to start a hive.
I'd like to create my own ant hive in artificial environment.
I unfortunately only ...
asked Jul 10 '14 at 19:12
Tomáš Zato - Reinstate Monica
9251 gold badge7 silver badges23 bronze badges
4
votes
2answers
248 views
Short, concise, practical manual for doing experimental biology
I am am physical scientist working in biology, and have recently started doing experiments. I would like a manual akin to "Numerical Recipes", but for the lab: straight forward, easy instructions on ...
4
votes
2answers
548 views
Transmembrane Protein Problem
Problem
A transmembrane protein has 1000 aa. The 5th aa is found on the external side of the cell membrane. It interacts with the aqueous environment outside the cell. Amino acid 90 is inside the ...
4
votes
1answer
44 views
Has there ever been a published experiment to take a viral “census” of a human subject's blood?
Has there ever been a published experiment to take a viral "census" of a human subject's blood?
By a "census", I mean to identify every active virus in the subject's blood with some probability.
So,...
4
votes
2answers
3k views
Definition of a Katal (unit of enzyme activity)
I am very confused about what one 'Katal' actually is. From Wikipedia,
"The katal is not used to express the rate of a reaction; that is expressed in units of concentration per second (or moles per ...
4
votes
1answer
261 views
What microscope/magnification would I need to observe P. Acnes bacteria?
I am currently attempting to grow a culture of P. Acnes bacteria. Right now, my only hope in identifying colonies of the bacteria in the culture is to use a black light to find colonies that glow ...
4
votes
2answers
1k views
Measuring the Rate of Photosynthesis
Update: I have just received a grant to pursue this research. However, I must still perform the preliminary experiment described below before dipping into my very limited funds. Thank you for any ...
3
votes
1answer
669 views
Appropriate statistical test for a student lab?
I am a HS Bio teacher and doing a microevolution lab involving candy. Essentially students use four candy types and push them together until one cracks (Nat Sel). They also do simulations of migration,...
3
votes
0answers
54k views
Experiment to show that carbon dioxide is needed for photosynthesis
In my school test, the question is as follows:
Will the experiment work satisfactorily?
What alterations can you make to obtain expected result?
What I wrote:
Yes, it will work satisfactorily. Lime ...
3
votes
0answers
41 views
How to quantify the Abundance of a particular gene in Bacteria and Archaea?
For 16s rDNA metagenomic analysis, do I need to do the following steps?
DNA extraction-> Purification-> PCR amplification-> Clone -> Pick every single
colony-> PCR amplify -> Sequence -> ...
3
votes
0answers
258 views
What is this red liquid produced by a fungal colony?
In a jar, where I had some orchid plants, a fungi start growing few days ago. I removed the plants and let the fungi to expand and grow more. After some days, red drops started to appear on the ...
2
votes
2answers
41 views
Monitoring fish growth
I am new to experimental design and would like to perform the following experiment but I am not sure if my setup is correct or not so far. My idea is below.
Let's say you want to optimise the growth ...
2
votes
1answer
924 views
Which wavelength to use to determine algae using a colorimeter?
I am doing a Biology project involving the measurements of the density of algae (Nannochloropsis) using absorbance colorimetry. What wavelength should I set my colorimeter to?
The issue is that there ...
2
votes
1answer
116 views
Suggestions for self-studying experimental techniques
I would appreciate any suggestion regarding self-studying experimental techniques used in biology for a theoretical scientist (candidate).
Maybe I should be more specific. I'm a biology major, but ...
2
votes
1answer
113 views
What advantages could there be for using the unseen variant chosen by He Jiankui instead of the naturally occurring CCR5-∆ 32 mutation?
Jennifer Doudna mentioned in https://youtu.be/9Yblg9wDHZA?t=2566 on 2019-02-21 that He Jiankui introduced an unseen variant of the CCR5 gene when gene editing the twin humans Lulu and Nana. What ...
2
votes
1answer
32 views
Fluorescent protein tags and colocalisation
We want study if 2 proteins A and B are co-located, for that we use 2 FTP(Fluorescent tag proteins) for each protein?and after the expirement these 2 FTP are co-located. Does that mean necessarily ...
2
votes
1answer
754 views
How does blue, yellow and orange coloured light affect photosynthesis? [closed]
I've been conducting an experiment with different colored light and photosynthesis, and so far my hypothesis states that red light will grow the plant, green will slow or kill it, and white will grow ...
2
votes
1answer
5k views
Why does the pH decrease, when adding pancreatin to different types of milk (cow milk, soy milk etc.)?
I'm experimentally observing how pancreatin affects the pH in different types of milk.
Why does the milk's pH decrease when I add an enzyme solution to different types of milk (e.g., cow milk, soy ...
2
votes
1answer
2k views
How do detergents interfere with protein assays?
This has been getting me stuck. I've tried to understand what a detergent would do in an assay, but I can't figure out whether it would affect the protein or the reagent (say, in a Bradford assay).
2
votes
1answer
2k views
What causes skewed ladder bands on an agarose gel?
I frequently ran into an issue where the bands of my ladder become skewed. What causes this?
Here's an example: 0.7% agarose gel, 100V, 60 minutes. Same problem occurs with 1%. I loaded 10 ul of a 20 ...
2
votes
0answers
21 views
Can we see the cell cycle of the cells administered using IVIS-like device?
My question;
Can we measure the cell cycle of the administered cells from outside the body, while the recipient is still alive ? Preferably, by using a device such as IVIS that can read fluorescent ...
2
votes
0answers
44 views
Veritasium video about mosquitoes
In the recent veritasium video about mosquitoes , the host says that the researchers used identical female twins and fraternal female twins to understand the effect of genes on attractiveness to ...
2
votes
0answers
56 views
plausible science for why an enzyme breaks down a substrate better at 4 degrees rather than 20 degrees [closed]
An enzyme was found to have an optimal temperature of 20 degrees. The same enzyme in sheep liver extract was found to have an optimal temperature of 4 degrees. What are some of the factors that can ...
2
votes
0answers
105 views
How would you identify the independent variable in an experiment where it isn't specified? [closed]
I know that the above question is broad, but I specifically want an answer in terms of the following investigation - all of question 3 (ONLY) of this paper.
The independent variable, according to ...
1
vote
2answers
101 views
Can one conduct “novel” research without an advanced laboratory? Has it been done by school pupils? [closed]
I want to conduct an experiment but as a secondary school pupil I will be using my school’s laboratory and to make novel or interesting discoveries, wouldn’t one need advanced equipment, a prospect ...
1
vote
1answer
53 views
Did Mendel believe that proteins are hereditary substances?
Scientists first thought that proteins, which are found in chromosomes along with DNA, would turn out to be the sought-after genetic material. Proteins were known to have diverse amino acid sequences, ...
1
vote
1answer
187 views
Was the Okazaki fragment experiment ever replicated?
Prof said that the experiment "proving" the existence of Okazaki fragments was never replicated.
Is this true? I tried looking on Google Scholar and I cannot find any experiments talking ...
1
vote
2answers
314 views
Can we directly stimulate M cones in retina with current technology?
I wonder if we could try to see imaginary "supergreen" color by directly stimulating M cones. For this I suppose we'd need:
Non-destructively scan specific human's retina to locate exact positions of ...
1
vote
1answer
77 views
Why didn't these scientists restore electrical activity in this pig's brain?
This experiment was published in Nature Magazine: Pig brains kept alive outside body for hours after death. The researchers used a system called BrainEx to revive certain metabolic and physiological ...
1
vote
1answer
58 views
How are fMRI responses to auditory stimuli measured?
How can fMRI experiments be conducted which measure the response to auditory stimuli (e.g. music) when the apparatus makes such loud, nasty, and distracting noises? Are there quieter MRI tomographs in ...
1
vote
1answer
3k views
Importance of fate maps
I did some normal google.book search and found the two importance of fate maps-
1. They helped establish the idea that communication between different parts of an embryo leads to the formation of new ...
1
vote
0answers
31 views
DNA replication in $ E.coli $
$ E.coli $ has circular DNA which I guess implies one strand forms the outer circle and the other the inner one. So, is there a way to know if the replicated DNA forms the outer or inner circle?
In ...
1
vote
0answers
377 views
The effect of sugar on rate of yeast Respiration
In my experiment, I am investigating the effect of different types of sugar on the rate of respiration of immobilized yeast (yeast trapped in calcium alginate balls). I am looking at glucose, fructose,...
1
vote
0answers
653 views
Is the human eye supposed to see the lense effect?
Just as the GoPro cameras, we need lenses to be able to focus. Nevertheless, we can see the "fisheye effect" on the shots taken by GoPros. I am talking about this:
Is it possible to notice the "fish ...
1
vote
0answers
410 views
How can I test a germinating pea seed for the presence of maltose?
I need to do a simple experiment to test whether germinating pea seeds produce maltose for the breakdown of starch into simple sugars.
1
vote
0answers
591 views
Wet weight measurements: Setting up cultures for measuring growth rate via wet weight
I have some isolates which I am trying to grow in some toxic leachate in order to measure their growth rate I also want to measure the growth rates of certain combinations of these bacteria.
I've ...
1
vote
0answers
22 views
experimental procedure regarding AceCS1 overexpression
I'm new to biology and was just as to curious on how one can experimentally prove/disprove that AceCS1 overexpression in mammalian cells would yield enhanced rates of fatty acid synthesis through ...
1
vote
0answers
28 views
Unsure why Synechococcus moves at angle in cuvette
I'd really appreciate your help. I'm working with Synechococcus. In my experiment, I have Synechococcus in a solution of milli-Q water. I illuminated it with a focused white light through a pinhole ...
0
votes
1answer
33 views
Can I imbibe seeds before sterilization?
For an experiment I want to germinate lettuce seeds. For logistical reasons it would be convenient to imbibe the seeds by placing them in the cold for 2 days, afterwards I would sterilize the seeds ...
0
votes
1answer
168 views
RNA interference and the former Petunias experiment >
I'm having problems trying to understand how RNA interference works.
From what I understood, in simple terms there is this dsRNA which is fragmented into siRNA by the enzyme called Dicer, and those ...
0
votes
0answers
10 views
How much mice red blood cells should I dilute before counting?
I am wondering how much red blood cells I should dilute with how much PBS before counting on hemocytometer with trypan blue
0
votes
0answers
25 views
Microarray experiment validation meaning
I have a quick question: what does microarray experiment validation mean ?
I was reading a paper in which they say that the data of the experiment show that the 3 genes radB, dp1 and dp2 are co-...
0
votes
0answers
45 views
question regarding indirect ELISA
what are the plausible reasons as to why the HRPO-conjugated donkey anti-human immunoglobulin and the chromogen/substrate were added from the right to the left of the microtiter plate?
0
votes
0answers
33 views
Ideas for microbiology lab. Antimicrobial properties of cutting boards
I am an undergraduate student in Biology and I am trying to design a microbiology lab that will takes 3 weeks to carry out (1-2 day each week). I am going to examine the antimicrobial properties of ...
0
votes
0answers
40 views
This 5X binding buffers where experiment participates in?
I saw 5X bingind buffer maded by;
100mM HEPES, pH 7.6
5mM EDTA
50mM ammonium sulfate
5mM DTT
1% Tween-20
150mM potassium chloride
which reaction helps this buffer? I don't consider to this buffer ...
0
votes
3answers
210 views
Biology Intuition and Origin of the “facts” [closed]
I am a graduate physics student but also taking biology classes. I am new to the field of bio. Most of what we do in class seem like memorizations and stating-facts. I am wondering:
How can I develop ...
-1
votes
1answer
59 views
What data would Meselson and Stahl have expected if DNA replication was dispersive rather than semiconservative? [closed]
What data would Meselson and Stahl have expected if DNA replication was conservative rather than semiconservative?
Answer:
In the first generation, there would be two bands, one of light density and ...
-1
votes
1answer
42 views
Am I reading this respirometer correctly?
I want to double check that I'm reading these respirometer vials correctly. The volume O2 of the following vials should be:
Squirrels in Torpor:
E1: 0.4 E2: 5.0 E3: 0.3 E4: 5.1 E5: 0.4 E6: 0
Squirrles ...
-1
votes
1answer
132 views
bacterium ability to metabolize a compound toxic to most other organisms, do not know how the bacterium takes up the compound from the environment [closed]
A new bacterium is discovered that has a unique ability to metabolize a compound toxic to most other organisms. The microbiologists researching this bacterium do not know how the bacterium takes up ...
