Questions tagged [gel-electrophoresis]

A method for analyzing or purifying samples. Molecules are separated by their relative weight, charge, or stereochemistry, which affect their speed of movement through a gel.

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Agarose gel electrophoresis - wrong running conditions?

Please have a look at the electrophoregram. The DNA ladder used is GeneRuler 50bp (loaded according to the instructions), size of the gel is 6*7cm, lane width is 4mm, agarose percentage is 1%, TBE ...
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Can't resolve protein with native PAGE

This is a native gel. Let's call the left 2 lanes protein A and the right 2 lanes protein B. B is the same as A except it has a FLAG tag. They are both homotetramers of about 65 kDa. After ...
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968 views

Which DNA fragments do not have expected sizes on this gel electrophoresis?

The problem is such: After performing a PCR, the vector carrying the PCR fragment with two restriction enzymes (Nhe1 and Asc1). The DNA samples were then separated using agrose gel electrophoresis ...
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196 views

Why do I get such a strong background in a detection of DIG-labeled DNA (Southern Blot)?

I have done a Southern Blot Analysis of DNAmt transferred to nylon membranes. The DNA was firstly loaded on a 2% agarose gel. An immunoassay was done to detect the bands using Anti-Digoxigenin ...
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81 views

Number of DNA strands per chromosome

As I was reading Griffith's Introduction to genetic analysis this evidence was provided for single DNA makes single chromosome. Eventually geneticists demonstrated directly that certain chromosomes ...
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17 views

How do you interpret the resulting bands of gel zymography?

I would like to clear up some things for gel zymography. I understand that bright bands show proteolytic activity. But which molecular weight do these bands correspond to? Is it the weight of the ...
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53 views

What is 'exhausted buffer' in gel electrophoresis?

For gel electrophoresis of DNA, we use TAE or TBE. TAE buffer is cheaper than TBE but it has low buffering capacity; it exhausts faster than TBE buffer and there is a need to change the buffer during ...
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34 views

What word/expression is used to describe a band's share in the stained portion of the electrophoretic lane?

I was translating the following sentence from Russian, and realized I was not sure how to put it right: The study shows that the electropherograms of the studied samples of DRUG NAME obtained in ...
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Which fractions are enriched for siRNA cleavage activity by comparing electrophoresis?

Size exclusion column chromatography was used to separate the proteins in a Drosophila cell lysate to attempt to identify the protein complex responsible for processing long dsRNA into siRNAs. SDS-...
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808 views

An unknown band appearing in my gel electrophoresis

I am having trouble understanding what is the source of band "2" in the following gel-electrophoresis: In this experiment, we took an E.coli transformant colony and ran its nucleic acids in the gel, ...
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Protein A-antibody SDS-PAGE

I wanted to know whether the boiling of Protein A- antibody complex for SDS-PAGE analysis will disrupt the interaction? and result in dissociation of protein A and antibody? Thank you
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38 views

Why is the capirally electrophoresis rarely carried out?

I'm a student at the university in Japan. I search details and advantages with each electrophoresis. I think the capirally electrophoresis has much nice method at the points of resolution, rapidity ...
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56 views

What are the advantages of iTRAQ/TMT technology over electrophoresis?

I'm currently working on the paper regarding proteomics research. I've listed a lot of questions that freshmen may have during their study. Can anyone explain (based on your experience in the lab) the ...
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3answers
221 views

What causes DNA gel electrophoresis bands to swirl?

I recently ran a gel on a new system and came out with something I've never seen before. I loaded the first three wells with a ladder in the first and an uncut plasmid in the next two. There are no ...
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117 views

Gel electrophoresis bands

Is there any way to calculate the number of bands formed due to sodium dodecyl sulphate-polyacrylamide gel electrophoresis for a particular compound of group of proteins? For example, how could I ...