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Questions tagged [gene-annotation]

Gene annotation refers to the characterization of a gene's exon-intron-UTR structure.

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Overlapping annotated regions among repetitive families of human genome

I am studying the repetitive elements in the human genome and I have come to know that some genomic locations are not uniquely described by a single repeat family. What's behind this uncertainty? Does ...
AVO's user avatar
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2 votes
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Why is SF3B3 gene annotation missing in chm13_v2 gff3 file, and is it temporary?

In the annotation gff3 provided for CHM13 v2, I noticed that for the SF3B3 gene there's no entry with type "gene" (third column). However, transcripts and other annotations are still ...
gc5's user avatar
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DNase annotations of Drosophila melanogaster

I am familiar that the RedFly database provides annotations for 1,458 sites in the genome of Drosophila melanogaster subject to cleavage by DNase I. It seems the vast majority of those annotations ...
arara's user avatar
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Can a gene be split between different genomic locations several kb appart? [closed]

Can you have exons and introns of the same gene separated by hundred of kb in a genome? If so, how is the full mRNA assembled in such distance? I'm working on plant mitochondria and I've seen an ...
LauraR's user avatar
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1 vote
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Where do I get an annotation file for yeast genome with ENSEMBL ids?

Even the annotation files hosted on the Ensembl site have systematic names (starting with Y..) instead of ENSEMBL ids (starting with EN..). I bet there would be a place where I can get an annotation ...
user345394's user avatar
5 votes
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How to categorize non-coding sequences in the genome?

I'm trying to wrap my head around the diversity of functions in a genome. Obviously, you've got coding and non-coding regions. But within "non-coding" regions, it seems to be a "wild west" to me. ...
CephBirk's user avatar
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Reason for name "Motile sperm domain-containing protein 2" if it has nothing to do with sperm

I am interested in a gene with the name "motile sperm domain-containing protein 2". The entry for the human gene in uniprot suggests that it does not have any relation to sperm or spermatogenesis. ...
Farbod Emami's user avatar
2 votes
2 answers

Get protein annotation from Uniprot out of protein mappings

Having a file of for about 10k mapping identifiers how can I get the annotations of the proteins from Uniprot ? As it is not possible to do It manually My mappings are contained in a .csv file They ...
A M's user avatar
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Use of pipe character in VCF info field

While annotating my VCF file with ClinVar, I noted the following value for the CLNSIG field (i.e., clinical significance): ...
Eleanore's user avatar
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Blastx: from gi to description

I have done a blastx and use the outfmt 6 option, because I want to work in the tables format. When I do this I get subject ids of the type sp|Q7XJS0|ASHR1_ARATH, sp|Q39547|CUCM1_CUCME, et cetera....
user30748's user avatar
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How to annotate the SwitchGear gene models?

According to this page(, the SwitchGear gene models can be annotated by the NCBI annotation associated with Refseq ...
Pugly's user avatar
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How valid are GO (Gene Ontology) terms?

I'm looking for papers quantifying the validity of ontologies such as GO. I'm relatively new to using annotation terms, and I often hear things like "annotation is messy" and "GO is good but it's ...
R Greg Stacey's user avatar
2 votes
1 answer

Is there a free software for manual sequence annotation?

I'm looking for a software that would allow me to make a quick graphical annotation of the structural features of a sequence. Something that would look like what you get in jalview, with your sequence ...
cachemoi's user avatar
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How many genes does D. melanogaster have?

Obviously there is no 100% exact number, but I came across this on flybase, the gold standard for annotation. I am confused now. "Genes located to the genome", is that what I am looking for? If so, ...
Dahlai's user avatar
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How to annotate a .broadpeak file with 9 columns?

I have this peak file I retrieved from an ENCODE chip-seq matrix. Its .broadpeak file looks like this: ...
Xanthoula Atsalaki's user avatar
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No genes' ID in peak annotations during ChiP-Seq with ChIPpeakAnno R package

I am trying to perfom Chip-Seq Analysis which is greatly described in that conversation: introduction to Chip Seq . The same as autor of mentioned question I am a student of Applied Mathematics and I ...
Marcin's user avatar
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3 votes
1 answer

In this illustration about CRISPR function, what do these objects mean (image provided)?

In the paper on CRISPRs, the following figure is shown: I added the light-green boxes. What do the red arrows and the line with a filled circle on the far left mean? What does the arrow in the ...
TMOTTM's user avatar
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What is the convention for indicating genomic primer sites?

What is the most common notation for indicating genomic binding sites of primers relative to an ORF? For instance, if I want to indicate a primer like so: ...
Superbest's user avatar
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How Scientists sequence, assemble and annotate plant genomes? [closed]

I previously read this question and this paper and learned good things about this topic. my current question is that scientists use which tools, algorithms, soft wares... to sequence, assemble and ...
MySky's user avatar
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Drosophila biarmipes gene annotation, transposable element?

When doing an annotation of a contig31 of Drosophila biarmipes genome, the gene predictions(in orange, red, green, tan, blue, brown, and gray) picked up numerous possible genes in the area circled in ...
Ro Siv's user avatar
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1 vote
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RNA-Polymerase III transcribed genes

I have a list of differentially regulated genes from a high-throughput experiment of the human genome. I would like to find out which of these genes are transcribed by a RNA polymerase III and which ...
Assa Yeroslaviz's user avatar
1 vote
1 answer

Is there a rule for the annotation of the basepair or gene locus origin in bacterial genomes?

Since all bacteria have one circular chromosome, there is no designated origin to start the systematic gene locus numbering. Therefore, what is the rule to start the numbering at a particular point (i....
user152314's user avatar
3 votes
1 answer

Understanding these SNP annotations

I am looking at the PharmaPGKB database for SNPs and trying to understand what the following annotation means: rs1801131 at 1:11854475 in CLCN6, MTHFR (VIP) Ok, ...
Aslan986's user avatar
  • 247
4 votes
2 answers

Combining gene expression data from two species

I currently have two sets of gene expression data. The first is a dataframe of genes identified by Annotation id CG numbers (for example "CG10005") in one column and a numerical variable of interest ...
rg255's user avatar
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6 votes
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Question: Annotation search in scientific literature

In a scientific paper I have been studying ("Predicting Novel Human Gene Ontology Annotations Using Semantic Analys" S.Draghici et al., 2010), I have read that authors checked the accuracy of their ...'s user avatar
10 votes
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Overlapping genetic information in eukaryotes

In my research, I look at a lot of gene predictions / annotations. Frequently, I see loci where multiple gene models overlap. I haven't taken a systematic approach to analyzing these cases, but I do ...
Daniel Standage's user avatar
9 votes
3 answers

What are the limiting factors for gene length and number of exons?

I recently downloaded gene annotations for Homo sapiens from Ensembl for some bioinformatic analysis. The vast majority of the gene annotations have 20 exons or less, although there are some that have ...
Daniel Standage's user avatar