Questions tagged [gene-editing]

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Limits of gene editing

I was reading some articles about CRISPR and the world of gene editing, but then a lot of questions for which I couldn't find any answer online came into my mind. Those are all about how far can we ...
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Gene knockdown vs gene knockout vs knocksideways?

How are the techniques: Knock-sideways, knockout & knock-down different?
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Can base editors be anything else than SpCas9 and SaCas9 variants?

I found out that only variants of Streptococcus pyogenes Cas9 (SpCas9) and Staphylococcus aureus Cas9 (SaCas9) are used with adenine or cytidine base editors, and I was wondering why. Can Neisseria ...
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4answers
61 views

Can the activity of a specific gene in a cell artficially be increased?

While there are many gene regulation mechanisms from the cell itself, I was wondering whether it is possible to increase the gene activity in a living cell permanently (so that the protein that it ...
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Is it possible to reduce maturity time of plants with biotechnology?

I've heard of speed breeding which makes use of optimal circumstances in glasshouses and growth chambers. Is it also possible to shorten a plant's maturity time (i.e. the time it needs from being ...
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How to design a permanent vaccine to cholera using CRISPR?

I know that Vibrio Cholerae infects the body through the GM1 ganglioside. So, would it be possible to engineer a CRISPR gene editing tool to prevent Vibrio Cholerae from getting into our cells? ...
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Is it possible to assemble chromosomes from different parent cells?

Assuming that one or two parents are carrying a genetic defect/disease which is localised on a specific chromosome, would it be possible to assemble a new haploid cells taking a (half) chromosome from ...
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2answers
98 views

DNA described somewhere?

This is not purely biological question but how do the companies making DNA analysis know what your DNA means? Is there some database describing human DNA? I watched some documents about DNA, gene ...
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Is it possible to detect whether an organism has been gene edited (by humans)?

Is it possible to detect whether an organism has been gene edited? For example, if someone gene-edits a human embryo (e.g., as He Jiankui did last year), can one later detect whether the human has ...