Questions tagged [gene-regulation]
The processes by which gene products (largely RNA and proteins) are increased or decreased.
82
questions
1
vote
1
answer
40
views
What is the best way to learn about gene regulation?
For those of you who already have a decent knowledge of how gene regulation works, how should someone new to the topic acquire a detailed overview?
Is there a particularly good and up-to-date resource?...
- 150
1
vote
0
answers
17
views
Are there limitations in using DNase I hypersensitive sites (DHSs) to identify candidate enhancers?
Candidate enhancer regions are often defined in studies by DHSs and/or certain chromatin marks. I was wondering if DHSs are exhaustive for identifying possible enhancer regions, and if there is any ...
- 11
1
vote
1
answer
160
views
What's the term for "Amino acid regulating the expression of components used to synthesize it"?
In the tryptophan operon wikipedia page, it says under the Attenuation title:
A similar attenuation mechanism regulates the synthesis of histidine, phenylalanine and threonine.
If my reading is ...
- 197
2
votes
1
answer
201
views
Are all genes capable of being switched on or off?
Are all genes capable of being switched on or off or only some genes? Are there some genes that permanently do not have the functionality that enables them to be switched on or off?
Everything I have ...
- 25
1
vote
2
answers
54
views
Is there a resource/paper containing information about which co-activators are often associated with a given transcription factor?
Given a list of human transcription factors, I would be interested in which co-activators they are often associated with.
I imagine that one could combine co-expression, pull-down, and other kinds of ...
- 11
-1
votes
1
answer
84
views
what are the nodes and edges in gene regulatory networks
I am trying to find out how one can using gene expression data can infer gene regulatory network applying graph theory concepts. But I could not find a proper reference that
1)explain how one can get ...
- 13
2
votes
1
answer
85
views
qPCR - different results total RNA vs mRNA
I have performed qPCR on a tissue, where I have extracted total RNA and also purified mRNA. I ran the qPCR samples together, and have therefore been exposed to the same conditions except the ...
- 53
-1
votes
1
answer
58
views
If the AraC protein was a gene repressor when it binds arabinose, would there be high or low transcription levels when arabinose is present? [closed]
If the AraC protein were to function as a gene repressor when it binds arabinose, would there be high or low transcription levels when arabinose is present?
- 1
0
votes
0
answers
54
views
Do transcription factors need to undergo extensive post-translational modification?
Some proteins need additional processing to become fully functional, for example NiFe-hydrogenases need to be cleaved by endopeptidases for some of their subunits to be active.
Is it known whether ...
3
votes
0
answers
45
views
What is the "anatomy" of transcriptional regulation in Archaea?
I want to know what are the DNA elements that are recognized by transcription factors in Archaea and so what is the "anatomy" of transcriptional regulation in this case.
I found in this ...
- 443
2
votes
0
answers
49
views
How does the phosphorylation state of 4E-BPs affect translation of nuclear-encoded mitochondrial transcripts?
A 2020 review paper about mTOR (ref. 1) says:
because biomass accumulation demands vast reserves of energetic currency, mTORC1 enhances translation of nuclear-encoded mitochondrial transcripts ...
- 351
6
votes
1
answer
114
views
Which genetic oscillator should I use to generate oscillations in range of 2-20 mins?
I'm looking to phase-separate the expression of two enzymes and hence am looking for a multi-component genetic oscillator. However, repressilators and metabolators have a large period of around 45 ...
-1
votes
1
answer
56
views
Gene regulation and epigenetics in specialized cells
Gene regulation is aided by epigenetics. Epigenetics determines which genes are turned off and which are switched on, and it varies throughout our lifetimes. Is it feasible that epigenetic ...
- 1
3
votes
0
answers
46
views
Conservation Law in Gene Regulatory Network modelling
I was going through the GRN modelling from Chemical and enzyme kinetics
by D. Gonze & M. Kaufman (PDF). The gene has 2 sites for activator/repressor. It say the DNA $D_0$ combines with activator/...
- 31
3
votes
1
answer
55
views
What are the relative roles of coding DNA versus regulatory DNA thought to be in evolution?
Intuitively, once you have the idea that some DNA is responsible for turning on and off the DNA that codes for proteins, it's possible to imagine that the regulatory DNA is actually the most important ...
- 189
3
votes
0
answers
40
views
Review paper on gene expression regulatory mechanisms
I'm a junior grad student in bioinformatics with CS background. I am involved in a project around discovering gene regulatory networks in some data, which got me curious about the gene expression ...
0
votes
3
answers
245
views
Difference between viral and native RNA
I am a data scientist with really next to no training in microbiology. I hope you'll all forgive my ignorance to entertain these questions. The attention COVID-19 has gotten recently got me thinking:...
- 19
0
votes
1
answer
193
views
How to biologically interpret path weight summation in weighted and directed gene regulation network?
I've considered asking this question on Bioinformatics SE, Mathematics SE, and Stats SE, however I've judged that my question belongs on Biology SE because I am interested in a biological (domain-...
- 1,045
5
votes
1
answer
331
views
How do enhancers induce transcription?
DNA response elements are DNA sequences that are could be found upstream, downstream of genes that regulate gene expression at the transcriptional level.
One type -Enhancers- bind specific ...
- 75
0
votes
0
answers
88
views
Is there a difference between "genetic cross regulation" and "crosstalk"?
What is the difference between genetic cross regulation and crosstalk? I'm a physics major and learning about bioinformatics now. So it might seem trivial to many but from the article "Wanner BL. ...
- 257
2
votes
1
answer
66
views
Question about alternative polyadenylation
I know that alternative polyadenylation creates different transcript isoforms. My question is whether alternative polyadenylation ever results in differences in the terminal/last exon? The only case I ...
- 21
2
votes
2
answers
91
views
Can gene-gene interactions result in gene expression?
I am building a project on Inferring Gene Regulatory Networks using ARACNE and PCA-CMI algorithms, and the input to these algorithms is taken from the DREAM3 challenge.
The format of the input data ...
- 123
3
votes
2
answers
3k
views
Why are tumor suppressor genes recessive?
In my Intro. to Biochemistry course, we have been studying cancer. The professor has pointed out that tumor suppressor genes are "recessive" while proto-oncogenes are "dominant". Since only one ...
- 275
0
votes
1
answer
39
views
what happens when you put enhancer and caat box in prokaryotes
what happens when you put enhancer and caat box in prokaryotes
- 1
1
vote
2
answers
119
views
Finding targets of transcription factors
I'm trying to find an easy way to get a list of putative targets of a particular transcription factor (for example, STAT1). I'm interested in targets determined using both experimental and ...
- 11
1
vote
0
answers
32
views
response elements in transcription regulation
I'm studying eukaryotic gene regulation and I want to know whether there are some instances where transcription factors don't need to bind to response elements to initiate transcription. RNA ...
- 56
2
votes
2
answers
3k
views
What is the difference between a response element and a enhancer?
I have been confused as to the difference between a response element and an enhancer.
Wikipedia has the definition of response element as the following:
Response elements are short sequences of ...
- 596
1
vote
1
answer
29
views
What are some online databases that I can search to find the pathways for sythesis of Lignin, Cellulose, GAX?
I am trying to obtain the pathway information for synthesis of Lignin,GAX and Cellulose in Sorghum. I am quite new to biology and genetics (EE background). I would appreciate it if y'all can provide ...
- 31
4
votes
1
answer
67
views
Can the same target gene be regulated differently by the same transcription factor under different conditions?
I'm very new in biology and doing more computational analysis. I'm confused with the type of interactions between Transcription Factors (TF) and target genes.
Is it possible that the same ...
- 43
3
votes
0
answers
4k
views
What is the difference between "dysregulation" and "deregulation" of miRNA?
I've started to study the role of miRNA in cancer. Wikipedia says:
Just as miRNA is involved in the normal functioning of eukaryotic
cells, so has dysregulation of miRNA been associated with ...
user30574
1
vote
0
answers
401
views
How is the breakdown of lactose regulated at the gene level?
How is the breakdown of lactose regulated at the gene level?
This is my answer: Without lactose in the cell, the repressor protein binds to the operator and prevents the read through of RNA ...
- 11
2
votes
0
answers
183
views
Consensus sequence for selection of initiation codon in eukaryotes
In bacteria the AUG (or other) codon at which translation of mRNA is initiated is preceded at a precise distance by a sequence known as the Shine and Dalgarno sequence, to which the 30S subunit ...
- 245
1
vote
0
answers
103
views
Length of upstream and downstream of tss important for gene regulation o
I want to find SNPs in the upstream and downstream regions of transcription start sites of some 42 genes implicated in a disease (on which I am working).
Are there any suggestions on what length ...
- 11
11
votes
2
answers
1k
views
Books on machine learning applications in Biology
I have recently engaged with a collaboration, which requires me to construct, then train an unsupervised artificial neural network (ANN).
However, I have only a very coarse understanding of what ...
- 3,577
2
votes
1
answer
97
views
Finding Regulatory Elements: How Have People Been Doing This?
First up, I am NOT asking about how to search for and match regulatory elements using a database. I'm asking about how people found what to search for and match in a genome, how they built those ...
- 183
0
votes
1
answer
51
views
On enhancers, strands, and zygosity
I came across a passage on enhancers that sounds out-of-whack to me:
...an enhancer sits on just one of DNA's two strands (usually the same strand as the protein-coding DNA gene itself). This is ...
- 179
1
vote
1
answer
34
views
How do messenger RNAs regulate each other's expression levels?
I know that researchers learn gene regulatory networks from microarray experiments that measure the mRNA expression levels. However, I do not understand how mRNAs regulate each other's levels. Do they ...
- 393
1
vote
1
answer
441
views
Can a miRNA be upregulated and downregulated in the same disease
I am computational guy and hence apologies if the question is silly.
Can the same miRNA show upregulation and downregulation in the same disease? (Not in the same experiment, though). For e.g. can ...
- 111
2
votes
1
answer
596
views
Can the brain influence gene expression?
A neuroscientist told me (according to my hazy memory) that the brain/nervous system can have an epigenetic function, ie directly regulate gene expression.
I'm not a biologist, but she talked me ...
- 23
1
vote
1
answer
394
views
How are constitutive enzymes regulated?
I found that inducible enzymes can be regulated by many ways, but
I cannot find how constitutive enzymes are regulated...
- 41
0
votes
1
answer
84
views
Are the subordinate genes of a repressed operon really "turned off"?
Operons are often described using all or nothing language. A repressor binding to the operator is usually presented as "turning off" the regulated genes. Case in point, Scitable at Nature.com says:
...
- 839
4
votes
1
answer
77
views
How much time do the different mechanisms of gene regulation need to take effect?
I am thinking of the major regulatory mechanisms, like general transcription factors, activators, repressors, and RNA interference.
If non-active regulator genes using each of the different ...
- 839
0
votes
2
answers
175
views
Does a gene that regulates itself necessarily be a transcription factor?
I am not a biologist, so please pardon the overly noob question. Some genes are transcription factors (TF) and regulate other genes. My question is: if a gene is NOT a TF gene, can it regulate itself? ...
- 101
1
vote
1
answer
109
views
Transcription of Genes: Are Specific Transcription Factors + Enhancers Necessary?
I learned about transcription in my AP Biology class and we discussed how transcription occurs, but I was wondering whether transcription always requires the enhancers, activators (specific ...
4
votes
1
answer
154
views
Can genes be expressed sequentially?
As I understand it, any gene on an exposed/unpacked region of a chromosome is continuously being expressed. Regulatory genes may increase or decrease the amount of protein synthesised due to its ...
- 41
1
vote
1
answer
62
views
Ways to identify that proteins are regulating different genes experimentally
As part of my study I have been given this hypothesis:
HIF 1a and HIF 2a regulate different genes in multiple myeloma
What ways do we have to identify that these proteins are regulating different ...
- 11
23
votes
3
answers
2k
views
120-year-old gene regulation problem independently solved by a computer. How?
My Background:
I'm a mathematics graduate student with a physics background. I have a very little biology knowledge and a little knowledge of machine learning and statistics.
Topic:
I recently ...
- 335
1
vote
1
answer
922
views
Why is the gene regulation in eukaryotic cells needs multiple level of control than in prokaryotic cells?
That "eukaryotic cells are more complex" and "compartmentalized" are used to justify the need of more level of control of gene expression. I get the basic idea but can't convince myself why complexity ...
- 427
3
votes
1
answer
72
views
Is RNA polymerase affected by proteins bound to the coding sequence of a gene?
I am designing a synthetic gene construct to express genes in E. coli driven by either Ptet or PLacO. The construct would look like:
-Ptet-(Gene1)-PLacO-(Gene2)-
I want to express each gene using ...
3
votes
1
answer
571
views
Regulation of V. cholerae virulence factors
So I know that several different environmental signals, such as pH, bile, and temperature, regulate virulence gene expression in V. cholerae. Specifically, they control expression of the genes ...
- 189