All Questions
Tagged with genetics molecular-genetics
253 questions
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Layman definition of genetic polymorphism?
I am reading an article about Genetic Polymorphism and there are lines in the article about genetic polymophism that I don't quite understand like.
In this area, there are six different chemotypes ...
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2
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2k
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How does the size of insert affects the rate of Homologous Recombination in yeast?
When performing genetic knockouts in yeast using homologous recombination to replace a target gene sequence via a vector DNA, does the region between the flanking regions in the vector have to be the ...
- 420
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2k
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Can DNA & RNA be considered as nature's programming language?
The final frontier of Biological Sciences could be considered understanding the effects of variation in the DNA (and RNA).
If after fertilization the DNA of the zygote could be genetically ...
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Why is mRNA needed in the Protein translation?
The original question was to predict the basic requirements for information storage. Then the discussion moved to why is it necessary to include mRNA in the protein translation process. Why can't ...
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G>T transversion VS. T>G transversion?
So I'm reading about how mutations in DNA can be caused by oxidative damage. An example of a product of oxidative damage is given: 8-oxo-7-hydrodeoxyguanosine
My textbook says that this product ...
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RNAs arising from intergenic regions
Which type of RNA molecule is coded for in intergenic regions? I think it must be a non-coding RNA but I'm unsure which type.
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Centriole genes Knock-out Experiment in Common experimental animals?
Anyone know of any experiments that have knocked out the genes for producing centrioles in a worm, mouse, fish, fly or whatever animal? Are the genes for centrioles even identified? It has been shown ...
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Why is the DNA codon table "equal" to the RNA codon table
Before anything else please pay attention of the double quotes on the "equal" in the title - I know they are not equal, but you will understand in a bit.
If I look at the DNA codon table ...
- 261
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Examples of genes involved in plastic responses
Adaptive plasticity involves sensing the environment and responding adaptively to it. Intuitively, I would think that this process may ask for a more or less complex genetic machinery of regulation of ...
- 68.3k
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Ways to measure effectiveness of a gene therapy trial?
I understand that Fluorescence in situ hybridisation can be used to measure the effectiveness of a gene therapy experiment/trial.
But what are some other genetics techniques to measure the ...
- 173
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2
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2k
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Redundancy of the genetic code
One particular codon codes only for one amino acid, but an amino acid can be coded for by several different codons. Now according to the genetic code, the codon UUU ...
user9950
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At what rate do chromosomal rearrangements occur?
How often do chromosomal rearrangements occur?
I am interested about these kind of chromosomal rearrangements that are passed on to the descendants, i.e. germ line chromosomal rearrangements. The ...
- 68.3k
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338
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Why aren't introns found on the ends of pre-RNA?
We recently learned in genetics class that exons always cap the ends of nascent RNA. I have been trying to figure out the reason why introns can't instead be found on the ends instead of exons. The ...
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1k
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What is the point of DNA sequencing?
This is a very very basic question. I've looked at methods such as chromosome sequencing and shotgun sequencing. Wikipedia says that:
...
- 63
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806
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Why do we use DNA sequencing methods such as shotgun?
I am learning about DNA cloning for the first time. What I understand is that, in order to clone DNA, we break-up the original gene into shreds. Then try to piece it back together.
Why exactly do we ...
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Influence of temperature on protein binding and decay rates
For computer modeling purposes, I am looking for some referenced quantitative measurements of the effect(s) of temperature on the dynamic of biochemical reactions.
Question
In particular, my ...
- 68.3k
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Influence of temperature on transcription, protein binding and decay rates
I am the kind of biologist who doesn't know much about molecular genetics and about the dynamic of biochemical reactions.
Question
My question concerns the influence of temperature on the dynamic of ...
- 68.3k
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Why do oncogenes show genetic dominance?
As we know that tumor suppressor gene causes cancer only when both the alleles are recessive in nature.But in case of oncogenes if only one allele is dominant it can cause cancer.Why in case of ...
- 422
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What is wrong with this analogy: Individuals are to alleles what governments are to individuals
I'm an amateur when it comes to genetics, I'm hoping to learn something from the corrections. I am taking epigenetics into account (kind of); all 3 entities are susceptible to external physical ...
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Heterochromatin production limitations
Currently playing with some ideas for a project and needed some guidance. I am wondering, both in Drosophila melanogaster and in general, is the amount of heterochromatin a cell/nucleus can produce ...
- 16.1k
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2
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159
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Heterochromatin binding sites position information
I am working on a project and I'm trying to find data on the position of genes and heterochromatin binding sites such as HP1 in Drosophila melanogaster. Is this information available for the DGRP ...
- 16.1k
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What is the difference between "cistron" and "gene"?
I'm asking after reading the cognate wikipedia.en article on "cistron". I am still not sure about the difference between the two terms.
To me it seems valid to picture a "cistron" as the genome wide ...
- 150
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What is the close and related genome used for in Gene models?
all
I am a little bit confused about using the related genome or reference genome.
When we have a reference genome, we can do alignment. Also we can do the assembly.
Can you give some more reason ...
- 149
2
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1
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205
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Why does the stability of YAC increase with size?
From Lehninger Principles of Biochemistry :
The stability of YAC increases with size (up to a limit). Those with
inserts of less than 100000 are gradually lost during mitosis.
Why does this ...
- 8,621
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Book Recommendation: Complex Traits and Complex Genetic Architecture
I am looking for a book (or any good source of information) that offers an in-depth discussion and models about the evolution and analysis of complex traits and complex genetic architecture. Do you ...
- 68.3k
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396
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What is the current molecular explanation of Mendel's pea cross?
With reference to the simplest Punnet square for Mendel's pea plant experiments it occurred to me that I could not connect the little I know about genetics to the little I know about molecules.
The ...
- 2,809
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2k
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How does GC-content evolve?
Background
GC-content refers to the frequency of base pairs that are either C or G in the genome, or in other words the number of GC base pairs divided by the addition of the number of GC base pairs ...
- 68.3k
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Property of E.coli harboring F element
From Genetics by Ursula Goodenough
E.coli harboring an F element are endowed with a number of phenotypic
traits :
1.They are sensitive to infection by ssRNA phages and certain ssDNA phages.
...
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2
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1
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2k
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Understanding DNA supercoiling
While trying to understand DNA supercoiling, I came across these lines in the book Genetics by Ursula Goodenough :
All natural DNA is superhelical. The axis of the duplex itself follows
a helical ...
- 8,621
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0
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42
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Is it reasonable to assume that RNA polymerase would bind preferentially to the promoter of the long allele of the SERT gene?
I'm trying to come up with an idea for a school project (a hypothetical research study).
I'm looking at depression and the serotonin transporter gene, which is highly expressed in the human ...
- 21
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1k
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Sexual Differentiation in Monoecious plants with unisexual flowers
In monoecious plants having unisexual flowers (eg Zea Mays, Ricinus Communis etc), there must be some mechanism as to produce two sexually distinct flowers from the same genotype. Since both the type ...
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Gene Complementation Question
Hi everyone! Here is a diagram of a genetics complementation problem. My main concern is regarding number 3d. I'm assuming that in this case, it would be complementary interaction and thus exhibiting ...
- 103
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368
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Pedigree Diagram help
I am asked to fill the genotypes in the spaces provided but looks like I am aving a little bit of trouble. can anyone help? thanks in advance!
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1
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398
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PedigreeAnalysis Genetics [duplicate]
Hello! I need help with this exercise question. I am not really sure which pedigree diagram is autosomal and which is not. But, is question b 1/2. Can someone give an explanation? Thanks very much in ...
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Activation Of Embryonic Genome
Embryonic gene activation is a process by which the embryo begins to transcribe its newly formed genome.As the embryonic gene activation occurs during early stages the paternal genome may not have any ...
user3800
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Difference between mutation and DNA damage
What is the strict difference between mutation and DNA damage? As far as I understand it, a mutation is an alteration in the genetic sequence, having "tricked" the repairing machinery and thus ...
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3k
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what is knob heterochromatin?
I am reading a paper which discusses Maize Genome Structure. Descriptions of the structure is given in the papers introduction.
I know about heterochromatin "heterochromatin stains intensely, ...
- 158
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Smallest unit on which selection can act
Traditionally, the individual was considered to be the smallest unit on which Natural Selection (NS) acts. Today, we usually consider the gene as being the unit of NS. Of course, we should also ...
- 68.3k
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Is there variation of AT/CG ratio along species?
Chargaff's rules say that the number of Adenine of the number of Thymine in a genome are equal (nA=nT) and similarly nC=nG. This makes obvious sense knowing that C binds to G and A to T.
But what ...
- 68.3k
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119
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Is there are practical lower limit to gene length in E. coli?
Question is rather self-explanatory.
Putting aside other post-transcriptional factors like rate of degradation of transcript, what is the smallest gene ever reported to have successfully been ...
- 6,695
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215
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Prenatal Marketing
This is for a short story idea.
Is it possible to modify the DNA of a child to make their metabolism more susceptible (physical response, addiction, etc) to a certain type of chemical i.e. a chemical ...
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Knockdown of long noncoding RNA (lncRNA) - how is it done?
I don't work at the wet lab and don't know all the details about the knockdown techniques.
My question is:
How lncRNA knockdown is done?
For example - you have lncRNA that is functional in the nucleus....
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What is solid-phase bridge amplification?
During Illumina sequencing there is a step called bridge amplification by which DNA is amplified by isothermal enzymes. What is this stage, and how does it work?
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What's the difference between shotgun sequencing and clone based sequencing?
In a lecture during my undergraduate degree we were introduced to the race to complete the human genome. Celera were competing with Sanger and collaborators to sequence the human genome. Celera ...
- 11.3k
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How is the exogenous DNA protected from degradation during bacterial transformation?
During transformation, a bacterium can take up DNA from its environment. A small fraction of bacterial species are known to be naturally competent, meaning that they can engage in this sort of ...
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Gene & Protein nomenclature: N-Myc, c-Myc, et. al
Can someone explain (or point me to an explanation of) exactly what is meant by all the different symbols I see used for writing genes and proteins?
I think I know that for genes, we use an italic ...
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1
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68
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methods for targetted deletion of genomic regions?
I would like to know what are currently used methods for targeted deletion of genomic regions in mammalian organisms or cell lines. I have heard of Zinc-Finger nucleases as a recent genetic ...
- 1,790
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1
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305
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Paralogous genes in genome-wide association studies?
Has anybody tested if paralogous genes are over-represented among the genes identified by genome-wide association studies (GWAS)?
For example, if a GWAS study finds 200 genes associated to the ...
- 744
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1
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4k
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Co-transformation of plasmids from the same incompatibility group
Can two plasmids with the same origin of replication (for example pBR322 ori) and thus from the same incompatibility group be successfully co-transformed in E. coli? What are the mechanisms that would ...
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Bicoid regulation of hunchback
I'm learning about development via the example of Drosophila embryogenesis. I understand that bicoid regulates hunchback, among other genes. My question whether the regulation is direct or indirect? ...
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