Questions tagged [green-fluorescent-protein]

Green fluorescent protein is a protein of the jellyfish Aequorea victoria which emits bright green light when it excited with blue to ultraviolet light. The protein is widely used in cell biology to tag proteins and to follow up their localization in the cell.

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CRISPR/Cas9 edited E.coli on AFM?

I am doing CRISPR/Cas9 experiment on E. coli. I am introducing recombinant plasmid BPK764 (which carries Cas9 + sgRNA designed and added later in that plasmid) into compentent E.coli cells which ...
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Why do I see so many kinetochores?

I am analysing RPE-1 cells from humans and I do not understand why I see so many kinetochores by immunofluorescence (more than 100 in many cells). They are in prometaphase.
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GFP brightness calculation

So this question is part math, part biology, but I'm asking about the biology part here (or maybe the chemistry part). Basically I'm asking about my attempt to calculate the possible brightness per ...
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Clarification of the brightness of bioluminescent proteins

One basic thing I'm not too clear on, which I would like to be clear on, is the brightness per mass unit of GFP or luciferase. I was having trouble finding a resource that would state the brightness ...