Questions tagged [growth-media]
Nutrient solution for the growth of eukariotic cells or bacteria. It can contain everything the cell needs for its growth (complete media) or can be incomplete (selective media) to select for cells that are able to synthesize specific nutrients.
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Carbon dioxide concentration in alkaline media
I am running a batch experiment using penicillin bottles where I have bacteria growing for 21 days in a highly alkaline media (pH 10). I am measuring the CO2 and O2 content in the headspace over time, ...
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Can I reuse agar in Petri dishes?
Can I simply re-melt agar in a contaminated Petri dish to use it again? I've tried searching for the answer on the Internet, but I was only able to find information about reusing Petri dishes ...
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Resazurin not turning pink
I want to add resazurin to my media as an anaerobic indicator.
To my understanding, the resazurin is blue when first added to the media, then turns pink after autoclaving, and finally it turns ...
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Why isn't bacterial colony a monolayer?
I am an engineer entering the field of microbiology. I was watching bacterial colonies (lab strain E. coli to be specific) being grown on agar plates. I was surprised that the bacterial colony was not ...
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Is there any alternative to peptone and beef or yeast extract?
I am trying to start a small microbiology project and I need a growth culture for bacteria. I do not want to use the costly nutrient agars you can buy online. I would like to create my own nutrient ...
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How to create long term stock for Bacillus subtilis?
I am trying to create a long-term stock for Bacillus subtilis (Bacterial glycerol stocks). I have looked online for guides and step-by-step tutorials and I found that they don't say where the sample ...
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How pricey does the media need to be for a typical bacterial culture experiment?
I'm considering various lab supplies for a protocol we're going to be running, and have been struck by the remarkable difference in price for different quality levels of the same basic substance.
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Medium preparation, using calcium hydroxide instead of potassium hydroxide
As the question states, I am interested in growing alkaliphile bacteria. I have seen many media use KOH, mainly to neutralize, and was wondering if it is possible to change this to Ca(OH)2?
Is there a ...
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Cell culture media L-Glut dilution?
Cross-posted on r/labrats.
I haven't done cell or tissue culture in about 4 years.
Protocol:
Volume: 200ml media (academia so using sparingly, not the whole 500ml bottle!)
1% L-Glut (250uM)
My L-Glut ...
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Would BMP7 introduced to a Petri dish with cartilage cause chondrocytes to replicate?
Chondrocytes are the cells that make up cartilage. As I understand it, the best growth factor that signals replication of chondrocytes is Bone morphogenetic protein 7.
If supplied with a sufficient ...
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Growing Mouth Bacteria in a bowl of sugar water
I want to teach my nephew why brushing in necessary and how sugar in mouth reduced by bacteria to acid.
I plan to put sugar mixed water in bowl and take swab from his mouth and dip in bowl, and test ...
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A question about microbes growing media
I was told in my microbiology course that blood agar can be put in GasPak system (anearobic) to grow strict anaerobes, but why would someone use blood-dependent bacteria in this experiment as these ...
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What’s this fungus growing in supersaturated salt solution
As a hobby, I’ve been trying to “grow” crystals of from supersaturated solutions of aluminium potassium sulphate.
I noticed that my solutions are also rather successful at growing a type of fungus.
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Autoclaving media Question
1.When I autoclave media should I close the cap tightly or slightly?
I put all tools in plastic bag when I autoclave, should I close slightly the plastic and when it finish I close tightly the ...
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Is it safe to autoclave seawater agar?
For an experiment I'm going to prepare an artificial seawater agar. Before use, I have to sterilize the medium of course. I imagine, that autoclaving will cause the salts in the seawater to ...
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Growth mediums required for human cell cultures
So i would like to try a culture some of my keratinocytes just to see if i can do it. Ive read about a few different types of growth medium such as DMEM and RPMI but i am not sure about when to use ...
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Major effects on cells grown with 5% FBS supplemented media instead of 10% FBS?
I maintain a few cell lines in the laboratory (industrial R&D) that I use regularly for experiments. I realized today that the media i prepared for the week on monday was only supplemented with 50 ...
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What is the practical difference between SC, SD and CSM?
Is there some logic to how SC, SD and CSM yeast dropout media differ from each other? I have encountered these three formulations in Sunrise Science Products' catalog, and it appears that the ...
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What culture medium should we use for tap/drinking water bacteria?
We are a group of high school students from Manila who is performing an investigatory project on a topic that we chose which, at first glance, seemed simple, but turned out a bit complicated. We're ...
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Difference between Peptone, Peptide and Proteose
In my school textbook, it is given that Pepsin converts proteins to peptones, proteose and peptides.
What is the difference between the three products? On googling the terms, the definition was ...
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How do absorbance readings indicate diauxic growth of bacteria?
How is growth rate an indicator of carbohydrate use? How do absorbance readings from a spectrophotometer show growth of bacteria if the absorbance readings are taken several times over the course of a ...
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Is casein hydrolysate the same thing as casamino acids?
Probably a dumb question, but these seem identical. If a protocol calls for one, can I use the other instead?
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Prevent clumping of bacteria in my media so that I can measure turbidity?
Forgive my naivety, I'm an undergraduate Chemical Engineering student doing research this summer into growth rates of various combinations of bacterial isolates.
I have about 9 isolates from a toxic ...
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Earliest to use media after autoclaving?
I am autoclaving media to use later to inoculate yeast. How much time do I have to wait before using the media and adding the culture? Can I use it a few hours after autoclaving?
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Can you grow more Biomass in a brickhouse only powered by solar cells than in a greenhouse? Will we ever be able to?
So I asked following question some physicists but they couldn't really help me though:
Right now I read that 1m² of solar cells generates roughly 100Watts of
energy. There are really efficient ...
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Is it essential to add cholesterol to agar or liquid culture for C. elegans?
In preparing agar plates with NGM, and also liquid cultures for growing c elegans, how essential is the addition of cholesterol and heavy metals? I have prepared both. I can't notice any obvious ...
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Can botulinum toxin be grown or kept from denaturing in an UNWRAPPED 50 pound hay bale?
Botulinum toxin is the neurotoxin protein created when botulism spores grow. The requirements for growth and/or for keeping the toxin from denaturing would seem to be very difficult to create in bale ...
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What is "bacto" peptone?
Standard recipes for yeast medium often include "bacto-peptone". Is this the same as bacteriological peptone? Is there an authoritative source that spells it out?
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Does yeast grow on LB agar plates?
I would like to grow E coli cells on cellophane on top of LB plate, and then grow yeast cells on the same LB plate. Does (or how well) yeast grow on LB plates? Is there a composition media that can be ...
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Generalism - specialism index in microbial ecology
I am doing research in Lipid metabolism in different yeast species and I would like to look into ecology of them.
I would like to classify them to a Generalist/specialist based on different ...
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Can someone explain the color-changing unit (CCU) to me?
I've been physically carrying out serial tenfold dilutions on samples of Ureaplasma to work out the color-changing units (CCU).
As a definition, the CCU is the highest dilution at which there is a ...
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How useful are vitamin and trace element additions in E. coli culture medium?
In protocols for minimal media for growing bacteria I often see that a vitamin mix or a mix of various trace metals is added. But at least for E. coli I know that those are not strictly necessary, ...
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Under what circumstances and why is terrific broth preferred to LB broth for E.coli growth?
Terrific Broth will result in a greater density of cells compared to LB but I have certainly seen it isn't as effective for the protein production of certain proteins compared to LB or defined media. ...
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Why is SOC medium recommended for transformations?
In pretty much every transformation protocol I've seen SOC medium is used to grow the bacteria for a short while after the tranformation and before plating.
I've usually substituted LB medium for ...
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